FastQCFastQC Report
Mon 15 Aug 2016
SRR2921612_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921612_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508601
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG106750.037.647941
CATGGGG79150.037.2960744
ATGGGGG48750.037.2342955
TACATGG110000.036.1423262
ACATGGG110400.035.4183773
GTATCAA44600.030.1702481
GTATACG800.005345349729.7697581
GGTATCA32100.028.1931691
TGGGGGG39050.028.1900986
TCAACGC49950.026.213174
CAACGCA50800.025.773715
ATCAACG51450.025.333263
TATCAAC53100.025.1064822
AACGCAG53150.024.8539626
ATGGGGA24550.023.0297955
GTGTAGC6800.021.0139481
ACGCAGA62600.021.0021137
AGTACAT75150.020.8285542
GAGTACA77600.020.5626161
CGCAGAG64800.020.2884068
Produced by FastQC (version 0.11.5)