##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921612_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1508601
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.966097728955503	31.0	31.0	34.0	26.0	34.0
2	30.079020231326904	31.0	31.0	34.0	26.0	34.0
3	30.373031040016546	33.0	31.0	34.0	26.0	34.0
4	33.746822387099044	37.0	35.0	37.0	30.0	37.0
5	33.82507501983626	37.0	35.0	37.0	30.0	37.0
6	33.840139970741106	37.0	35.0	37.0	30.0	37.0
7	33.75896940277781	37.0	35.0	37.0	29.0	37.0
8	33.76853190472497	37.0	35.0	37.0	29.0	37.0
9	35.39944027612338	39.0	37.0	39.0	29.0	39.0
10-11	35.41318347263458	39.0	37.0	39.0	30.0	39.0
12-13	35.340925798140134	39.0	37.0	39.0	29.0	39.0
14-15	36.62891911115	40.0	38.0	41.0	29.0	41.0
16-17	36.595723123609226	40.0	38.0	41.0	28.5	41.0
18-19	36.55869776037534	40.0	38.0	41.0	28.0	41.0
20-21	36.61342561750921	40.0	38.0	41.0	29.0	41.0
22-23	36.547060819925214	40.0	38.0	41.0	28.0	41.0
24-25	36.54110165643533	40.0	38.0	41.0	28.5	41.0
26-27	36.49153454094224	40.0	38.0	41.0	29.0	41.0
28-29	36.43579614490511	40.0	38.0	41.0	29.0	41.0
30-31	36.33842579979729	40.0	37.0	41.0	28.0	41.0
32-33	36.227866082549326	40.0	37.0	41.0	28.0	41.0
34-35	36.09929630167287	40.0	37.0	41.0	27.0	41.0
36-37	36.00114742068976	40.0	37.0	41.0	27.0	41.0
38-39	35.89181997095322	40.0	36.5	41.0	26.0	41.0
40-41	35.77588838930903	40.0	36.0	41.0	26.0	41.0
42-43	35.555612120103326	40.0	35.5	41.0	25.0	41.0
44-45	35.27650850025951	40.0	35.0	41.0	24.0	41.0
46-47	35.04963041917644	39.5	35.0	41.0	24.0	41.0
48-49	34.85893055884226	39.0	35.0	41.0	23.0	41.0
50-51	34.661114171341524	39.0	35.0	41.0	23.0	41.0
52-53	34.46752852477229	39.0	34.5	41.0	22.0	41.0
54-55	34.232110080796716	38.5	34.0	40.0	21.5	41.0
56-57	33.976845766375604	38.0	34.0	40.0	20.0	41.0
58-59	33.974467072473104	38.0	34.0	40.0	20.5	41.0
60-61	33.748710560313825	37.5	34.0	40.0	20.0	41.0
62-63	33.41890301013986	37.0	34.0	40.0	18.0	41.0
64-65	33.119218733117634	36.5	33.5	39.5	18.0	41.0
66-67	32.791242018267255	36.0	33.0	39.0	15.5	41.0
68-69	32.44987640867267	35.5	33.0	39.0	13.0	40.5
70-71	32.077549994995366	35.0	33.0	38.0	9.5	40.0
72-73	31.72564216780978	35.0	33.0	37.0	7.5	39.5
74-75	31.361085535539218	35.0	32.0	37.0	7.0	39.0
76-77	31.059260864867518	35.0	32.0	36.0	4.0	39.0
78-79	30.788199464271866	35.0	32.0	36.0	2.0	37.0
80-81	30.53269353526877	35.0	32.0	35.0	2.0	37.0
82-83	30.30855375278155	35.0	31.5	35.0	2.0	36.5
84-85	30.09340408762821	35.0	31.0	35.0	2.0	36.0
86-87	29.91312547187759	35.0	31.0	35.0	2.0	36.0
88-89	29.741283811955583	35.0	31.0	35.0	2.0	35.5
90-91	29.547231839300117	35.0	31.0	35.0	2.0	35.0
92-93	29.45824508932448	34.0	31.0	35.0	2.0	35.0
94-95	29.31912679363198	34.0	31.0	35.0	2.0	35.0
96-97	29.217829631559304	34.0	30.0	35.0	2.0	35.0
98-99	29.109950212150196	34.0	30.0	35.0	2.0	35.0
100-101	28.986549458736935	34.0	30.0	35.0	2.0	35.0
102-103	28.12977785378639	33.5	28.0	34.5	2.0	35.0
104-105	29.00735913604724	34.0	30.5	35.0	2.0	35.0
106-107	29.24647703402026	35.0	31.0	35.0	2.0	35.0
108-109	29.22155460588983	35.0	31.0	35.0	2.0	35.0
110-111	29.142278839799257	35.0	31.0	35.0	2.0	35.0
112-113	29.037046574939296	35.0	31.0	35.0	2.0	35.0
114-115	28.895639735092317	35.0	31.0	35.0	2.0	35.0
116-117	28.752224411888896	35.0	31.0	35.0	2.0	35.0
118-119	28.437640568977482	35.0	30.0	35.0	2.0	35.0
120-121	28.285485028844604	34.5	30.0	35.0	2.0	35.0
122-123	28.00004341770952	34.0	29.0	35.0	2.0	35.0
124-125	27.22663712936688	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	60656.0
3	10793.0
4	5496.0
5	4729.0
6	5021.0
7	5381.0
8	5535.0
9	5796.0
10	6460.0
11	6513.0
12	6486.0
13	6365.0
14	6447.0
15	5864.0
16	4963.0
17	4730.0
18	4273.0
19	4117.0
20	4301.0
21	4347.0
22	5073.0
23	6881.0
24	9859.0
25	12637.0
26	13804.0
27	15080.0
28	17947.0
29	21528.0
30	25639.0
31	32224.0
32	41579.0
33	57058.0
34	88358.0
35	142612.0
36	286291.0
37	448017.0
38	115654.0
39	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.747237344694916	16.726703491020334	11.148483564629261	25.377575599655493
2	16.830646086205377	19.489121405761388	38.08127836989806	25.598954138135177
3	20.247671450827006	23.910238987039676	28.11031190957605	27.73177765255726
4	13.925803650152806	14.639923629203874	34.405176242848526	37.029096477794795
5	14.865668514858573	38.09944965295849	32.04463771305976	14.990244119123183
6	34.43217610148023	32.98067653044095	17.068921016155862	15.518226351922959
7	30.372357712792418	32.08763074637359	18.92690141872666	18.61311012210733
8	28.464292796350787	33.992756492264064	19.422442671038503	18.120508040346646
9	28.895924571587706	15.709876440887557	17.799208982982854	37.594990004541884
10-11	27.173242260880926	25.332692669271108	25.973887302151034	21.520177767696936
12-13	26.616007897146165	22.76946392617839	26.311544802979064	24.302983373696378
14-15	24.510352898662436	24.011033366983778	23.58322517119456	27.89538856315923
16-17	24.88229101910564	26.964963322143483	24.242397115504165	23.910348543246716
18-19	24.722280892613732	26.44925722179099	25.609642472397194	23.218819413198087
20-21	25.524599908959832	25.466547711049248	25.347956784763603	23.66089559522732
22-23	25.460442465083027	24.956333064987803	25.135675852748594	24.447548617180566
24-25	24.95778624539445	25.041500936444137	25.23532116768235	24.765391650479064
26-27	24.518302147500165	25.300763759728547	25.4504783073071	24.73045578546419
28-29	24.68120409801575	25.926047507776538	25.199739637993357	24.19300875621435
30-31	25.472709882856464	25.373186778212993	25.16860414038659	23.985499198543952
32-33	24.9612850287192	25.361049639041532	25.202211336831027	24.475453995408245
34-35	24.92142235892416	25.714311293793006	25.347212205555593	24.01705414172724
36-37	24.95567447880844	25.707741594392893	25.300168882804613	24.036415043994054
38-39	25.049817124493583	25.78947420764169	25.188154745862217	23.97255392200251
40-41	25.00839723131761	25.501255854436938	25.24285655075227	24.24749036349318
42-43	24.827101976517298	25.41814767888009	25.056273491489193	24.698476853113423
44-45	24.397591659448473	25.54729814170067	25.493895115926996	24.56121508292386
46-47	24.2852733675835	25.431527900669433	25.49375371506677	24.789445016680297
48-49	24.433250372395925	25.731837141060225	25.43352256207068	24.401389924473165
50-51	24.461127871896032	25.6609753671717	25.601001226668433	24.276895534263833
52-53	24.473236044820478	25.606279561422614	25.32654110593826	24.593943287818647
54-55	24.172186637962042	25.287629153404755	25.664261317281024	24.87592289135218
56-57	24.427142057232917	25.545026854762163	25.185659381297842	24.842171706707077
58-59	24.371625167280982	25.750547547910234	25.46575965041797	24.412067634390816
60-61	24.904813826286933	25.60460832029099	25.47369314397653	24.016884709445552
62-63	24.61108411112091	25.607217992469323	25.47570630758816	24.305991588821605
64-65	24.345819193325905	25.297499568216637	25.469748809819396	24.88693242863806
66-67	24.50405051246815	25.437384066823927	25.309876221908738	24.748689198799184
68-69	24.200428842509776	25.444721882239367	25.525179579458523	24.82966969579233
70-71	24.129075257978087	25.349828898851367	25.689643738228508	24.831452104942038
72-73	24.256495532177144	25.812393210845304	25.225479685114134	24.705631571863425
74-75	24.087753828937974	25.655856652570492	25.354785418380626	24.90160410011091
76-77	24.08975535761227	25.18416714483992	25.48406547708431	25.242012020463495
78-79	23.962126850518086	25.34785844645163	25.761764496243018	24.928250206787265
80-81	24.23551001966108	25.532094043032945	25.373903698146833	24.858492239159137
82-83	24.31420847001646	25.540370076299435	25.497537631717226	24.64788382196688
84-85	24.300814506516186	25.68436844917588	25.086648218838793	24.92816882546914
86-87	23.947356033629447	25.80796793255547	25.271510507378736	24.97316552643635
88-89	23.879134416351622	25.328326661159196	25.637260037102504	25.15527888538668
90-91	24.352059013756595	25.06410539042213	25.616397984828197	24.967437610993077
92-93	24.30411256308057	25.2074014534426	25.662128456483728	24.8263575269931
94-95	24.212662762768936	25.167080237628177	25.527062656166404	25.093194343436487
96-97	23.990576448347316	25.065129179364053	25.594715014467813	25.349579357820822
98-99	23.880613340395566	25.16139446407956	25.458346356812278	25.4996458387126
100-101	23.73548955725437	25.42919354737131	25.548886780013387	25.286430115360943
102-103	23.58296357846918	25.26170093093044	25.702821494493534	25.452513996106845
104-105	23.711905719950654	25.298652261444442	25.598481167390442	25.390960851214462
106-107	23.681761385669354	25.217438858267847	25.810345456172506	25.290454299890296
108-109	23.648469467582096	25.174427392857428	25.842435910695293	25.334667228865182
110-111	23.405315917425796	25.774195312487052	25.68570164376161	25.13478712632554
112-113	23.28606987435079	25.8743242750561	25.647618763526932	25.191987087066188
114-115	23.381368295333967	25.896284127247732	25.566990557090868	25.15535702032743
116-117	23.222012707236708	25.75175976567614	25.59461800232746	25.43160952475969
118-119	23.225103556190888	25.46623135897164	25.798561729726565	25.510103355110903
120-121	23.24520642563679	25.658698035638768	25.862896415309212	25.233199123415233
122-123	23.072420652676108	25.8112050399413	25.647575317067805	25.468798990314788
124-125	22.901953456577125	25.664016640272795	25.722101991493005	25.711927911657067
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	742.0
1	575.5
2	364.5
3	297.0
4	249.0
5	211.5
6	206.0
7	230.0
8	251.0
9	271.5
10	288.0
11	307.5
12	339.0
13	366.5
14	420.5
15	477.5
16	524.0
17	559.5
18	618.0
19	696.5
20	752.0
21	812.5
22	883.5
23	1056.5
24	1334.5
25	1704.0
26	2249.0
27	2946.0
28	3779.0
29	4858.0
30	6406.0
31	8224.5
32	10245.0
33	12326.5
34	14531.0
35	17057.5
36	20201.0
37	23181.0
38	25634.0
39	28357.5
40	31293.0
41	34066.0
42	36948.0
43	41257.0
44	45573.5
45	49281.0
46	53800.5
47	58264.0
48	62910.5
49	68020.0
50	72633.0
51	73956.0
52	72135.5
53	70924.0
54	69908.0
55	67480.0
56	63217.5
57	57472.5
58	51753.5
59	45569.5
60	38952.0
61	32866.5
62	26917.0
63	20991.5
64	15856.0
65	12060.0
66	8963.5
67	6850.0
68	5508.5
69	4311.5
70	3400.5
71	2712.0
72	2132.5
73	1770.0
74	1363.5
75	999.0
76	728.0
77	432.0
78	275.0
79	190.5
80	128.5
81	87.0
82	69.0
83	65.0
84	51.5
85	41.5
86	36.5
87	35.5
88	32.0
89	20.5
90	19.5
91	22.0
92	16.0
93	14.5
94	12.5
95	12.5
96	10.5
97	5.5
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023863168591297498
2	0.011931584295648749
3	0.010009273492460896
4	0.011335005080866312
5	0.018560242237675835
6	0.09744127174779812
7	0.06038707385186673
8	0.050908092994768006
9	0.027575217038832665
10-11	0.052399541031724096
12-13	0.013787608519416333
14-15	0.02972953086999147
16-17	0.03569532301781585
18-19	0.04292056017462536
20-21	0.03151926851433878
22-23	0.03944051475506114
24-25	0.033309006158686094
26-27	0.048488632845928116
28-29	0.046433748883899714
30-31	0.02707806769318064
32-33	0.030723829561295532
34-35	0.046234889145638905
36-37	0.010506422838112928
38-39	0.022172860816080595
40-41	0.0439811454453497
42-43	0.022670010161732625
44-45	0.017201367359560283
46-47	0.025487189787094137
48-49	0.03059125640245499
50-51	0.029895247318542147
52-53	0.026945494534340093
54-55	0.02422774477810899
56-57	0.026216342160717113
58-59	0.018924818424487323
60-61	0.02469175083405089
62-63	0.02452603438550021
64-65	0.021311135283617073
66-67	0.030392396664194177
68-69	0.023531735694196147
70-71	0.04779262376201527
72-73	0.024658607544340752
74-75	0.010208133230721709
76-77	0.017002507621299467
78-79	0.02707806769318064
80-81	0.01932253790100895
82-83	0.02641520189897793
84-85	0.01345617562231498
86-87	0.018560242237675835
88-89	0.02300144305883398
90-91	0.022703153451442762
92-93	0.00752352676420074
94-95	0.012859596407532542
96-97	0.016737361303618387
98-99	0.007125807287679115
100-101	0.01070528257637374
102-103	0.0037451917372453023
104-105	0.0049052068771000415
106-107	0.0010605852707243333
108-109	0.015709919322604186
110-111	0.0012263017192750104
112-113	0.00238631685912975
114-115	0.014616190762169719
116-117	0.005998935437534511
118-119	0.28002765476093416
120-121	0.0012594450089851458
122-123	7.954389530432501E-4
124-125	0.31681670633918446
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1508601.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.61923816713366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.99163320647222	54.45847745254898
2	9.542492565819307	12.52342184769199
3	2.993626572741111	5.8931848518047705
4	1.3451932026052538	3.5308221257025365
5	0.7608951081347393	2.496467866045018
6	0.489621954966885	1.9277171804897768
7	0.3497714527777723	1.6066215384722287
8	0.24859473742462768	1.3050077825730158
9	0.21206085588007115	1.2523744627128532
>10	1.0127120737085524	11.526610328356778
>50	0.04117300048031387	1.7993573730531072
>100	0.011619109231460442	1.2744897987896262
>500	3.03079878841975E-4	0.16294795437283743
>1k	3.03079878841975E-4	0.2424994373864277
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.628657942027083E-5
2	0.0	0.0	0.0	0.0	6.628657942027083E-5
3	0.0	0.0	0.0	0.0	6.628657942027083E-5
4	0.0	0.0	0.0	6.628657942027083E-5	6.628657942027083E-5
5	0.0	0.0	0.0	6.628657942027083E-5	6.628657942027083E-5
6	0.0	0.0	0.0	6.628657942027083E-5	6.628657942027083E-5
7	0.0	0.0	0.0	6.628657942027083E-5	6.628657942027083E-5
8	0.0	0.0	0.0	6.628657942027083E-5	6.628657942027083E-5
9	0.0	0.0	0.0	6.628657942027083E-5	1.3257315884054166E-4
10-11	0.0	0.0	0.0	6.628657942027083E-5	1.3257315884054166E-4
12-13	0.0	0.0	0.0	6.628657942027083E-5	1.3257315884054166E-4
14-15	0.0	0.0	0.0	6.628657942027083E-5	1.3257315884054166E-4
16-17	0.0	0.0	0.0	1.3257315884054166E-4	1.988597382608125E-4
18-19	0.0	0.0	0.0	1.3257315884054166E-4	1.988597382608125E-4
20-21	0.0	0.0	0.0	1.3257315884054166E-4	2.6514631768108333E-4
22-23	0.0	0.0	0.0	2.982896073912188E-4	2.982896073912188E-4
24-25	0.0	0.0	0.0	0.001292588298695281	3.314328971013542E-4
26-27	0.0	0.0	0.0	0.0022537437002892085	3.314328971013542E-4
28-29	0.0	0.0	0.0	0.006065222016954782	3.314328971013542E-4
30-31	0.0	0.0	0.0	0.02025055001289274	3.314328971013542E-4
32-33	0.0	0.0	0.0	0.05074237654621733	3.645761868114896E-4
34-35	0.0	0.0	0.0	0.09107776012345212	3.97719476521625E-4
36-37	0.0	0.0	0.0	0.14841565132198642	4.6400605594189585E-4
38-39	0.0	0.0	0.0	0.24161458198688718	4.6400605594189585E-4
40-41	6.628657942027083E-5	0.0	0.0	0.3518823068525077	4.6400605594189585E-4
42-43	6.628657942027083E-5	0.0	0.0	0.4535659196832032	4.6400605594189585E-4
44-45	9.942986913040625E-5	0.0	0.0	0.5666176808844752	4.6400605594189585E-4
46-47	1.3257315884054166E-4	0.0	0.0	0.684077499617195	4.6400605594189585E-4
48-49	1.3257315884054166E-4	0.0	0.0	0.8086299823478839	5.302926353621667E-4
50-51	1.3257315884054166E-4	0.0	0.0	0.9362979343113256	5.302926353621667E-4
52-53	1.3257315884054166E-4	0.0	0.0	1.0760963303086766	5.302926353621667E-4
54-55	1.3257315884054166E-4	0.0	0.0	1.2326652308993564	5.302926353621667E-4
56-57	1.3257315884054166E-4	0.0	0.0	1.3907587228167024	5.965792147824375E-4
58-59	1.3257315884054166E-4	0.0	0.0	1.5675118868408546	6.297225044925729E-4
60-61	1.3257315884054166E-4	0.0	0.0	1.745524495873992	6.628657942027084E-4
62-63	1.3257315884054166E-4	0.0	0.0	1.9383521554075598	6.628657942027084E-4
64-65	1.3257315884054166E-4	0.0	0.0	2.131047241782287	6.628657942027084E-4
66-67	1.3257315884054166E-4	0.0	0.0	2.3524775603357018	6.960090839128437E-4
68-69	1.3257315884054166E-4	0.0	0.0	2.58941893847346	7.291523736229792E-4
70-71	1.3257315884054166E-4	0.0	0.0	2.837065599187592	7.622956633331146E-4
72-73	1.3257315884054166E-4	0.0	0.0	3.0668480267479605	7.9543895304325E-4
74-75	1.3257315884054166E-4	0.0	0.0	3.3143621143032522	7.9543895304325E-4
76-77	1.3257315884054166E-4	6.628657942027083E-5	0.0	3.6039681797904155	7.9543895304325E-4
78-79	1.3257315884054166E-4	6.628657942027083E-5	0.0	3.9190614350646724	8.285822427533853E-4
80-81	1.3257315884054166E-4	6.628657942027083E-5	0.0	4.221162520772557	8.617255324635208E-4
82-83	1.3257315884054166E-4	6.628657942027083E-5	0.0	4.533935745767105	8.617255324635208E-4
84-85	1.3257315884054166E-4	6.628657942027083E-5	0.0	4.863844051541793	8.617255324635208E-4
86-87	1.3257315884054166E-4	6.628657942027083E-5	0.0	5.238197508817772	8.617255324635208E-4
88-89	1.3257315884054166E-4	6.628657942027083E-5	0.0	5.659084144846782	8.617255324635208E-4
90-91	1.3257315884054166E-4	6.628657942027083E-5	0.0	6.085770856575065	8.617255324635208E-4
92-93	1.3257315884054166E-4	6.628657942027083E-5	0.0	6.49817281043828	8.617255324635208E-4
94-95	1.3257315884054166E-4	6.628657942027083E-5	0.0	6.9178994313274345	8.617255324635208E-4
96-97	1.3257315884054166E-4	6.628657942027083E-5	0.0	7.3912518949675885	9.61155401593927E-4
98-99	1.3257315884054166E-4	6.628657942027083E-5	0.0	7.930426931972073	0.0010605852707243333
100-101	1.3257315884054166E-4	6.628657942027083E-5	0.0	8.50755103569466	0.0010605852707243333
102-103	1.3257315884054166E-4	6.628657942027083E-5	0.0	9.0972695895071	0.0011600151398547397
104-105	1.3257315884054166E-4	6.628657942027083E-5	0.0	9.69189335019664	0.001193158429564875
106-107	1.3257315884054166E-4	6.628657942027083E-5	0.0	10.321682141268631	0.001193158429564875
108-109	1.3257315884054166E-4	6.628657942027083E-5	0.0	10.996048657000758	0.001193158429564875
110-111	1.3257315884054166E-4	6.628657942027083E-5	0.0	11.711943714739682	0.001193158429564875
112-113	1.3257315884054166E-4	6.628657942027083E-5	0.0	12.420712965190928	0.001193158429564875
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	10675	0.0	37.64794	1
CATGGGG	7915	0.0	37.296074	4
ATGGGGG	4875	0.0	37.234295	5
TACATGG	11000	0.0	36.142326	2
ACATGGG	11040	0.0	35.418377	3
GTATCAA	4460	0.0	30.170248	1
GTATACG	80	0.0053453497	29.769758	1
GGTATCA	3210	0.0	28.193169	1
TGGGGGG	3905	0.0	28.190098	6
TCAACGC	4995	0.0	26.21317	4
CAACGCA	5080	0.0	25.77371	5
ATCAACG	5145	0.0	25.33326	3
TATCAAC	5310	0.0	25.106482	2
AACGCAG	5315	0.0	24.853962	6
ATGGGGA	2455	0.0	23.029795	5
GTGTAGC	680	0.0	21.013948	1
ACGCAGA	6260	0.0	21.002113	7
AGTACAT	7515	0.0	20.828554	2
GAGTACA	7760	0.0	20.562616	1
CGCAGAG	6480	0.0	20.288406	8
>>END_MODULE