FastQCFastQC Report
Mon 15 Aug 2016
SRR2921612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508601
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28500.1889167513477719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23880.15829235165560673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.1029430578396806No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA15150.1004241678217103No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG46150.036.79835
CATGGGG79450.035.7296144
GTACATG109500.034.4320261
TACATGG112550.033.3332862
GGTATCA45300.033.054841
ACATGGG114800.032.160423
TGGGGGG34250.031.8442966
GTATCAA60500.031.6525881
CGTTATA1107.6430064E-427.1112982
TCAACGC69900.026.875014
CAACGCA71000.026.52065
ATCAACG73350.025.6956713
AACGCAG74500.025.5996996
TATCAAC74600.025.2651122
TACCGTA1200.001275591224.833047
GAGTACT34350.023.22588712-13
GTACCGT2153.3743345E-622.1764836
ACGCAGA86300.021.961277
ATGGGGA24200.021.6681545
GTACTTT37200.021.35972814-15
Produced by FastQC (version 0.11.5)