FastQCFastQC Report
Mon 15 Aug 2016
SRR2921611_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921611_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566902
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41200.036.9827731
GTACATG97100.031.1387561
TCAACGC48200.030.6171174
CAACGCA49400.030.1094885
CATGGGG74200.029.9935134
TACATGG100900.029.5465092
ATCAACG50000.029.2768753
ATGGGGG40950.028.6221715
ACATGGG104900.028.3631293
AACGCAG52550.028.3019246
TATCAAC52700.028.2285772
GGTATCA27750.027.8828491
ACGCAGA60300.024.6597237
CGCAGAG63550.023.2114148
GAGTCCG5850.021.3610743
TATAACG1954.0371193E-521.3610742
GTATATA4251.8189894E-1221.006761
GCAGAGT71550.020.2011439
GAGTACA76150.019.227461
AGTACAT74900.018.9878462
Produced by FastQC (version 0.11.5)