##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921611_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1566902
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.476285689851693	31.0	30.0	33.0	26.0	34.0
2	29.648747018001124	31.0	31.0	34.0	26.0	34.0
3	29.753216219010508	31.0	31.0	34.0	26.0	34.0
4	33.28286261680692	37.0	35.0	37.0	28.0	37.0
5	33.34480714173573	37.0	35.0	37.0	28.0	37.0
6	33.65393240930192	37.0	35.0	37.0	29.0	37.0
7	33.673107188579756	37.0	35.0	37.0	29.0	37.0
8	33.58221509705138	37.0	35.0	37.0	28.0	37.0
9	35.30138706824039	39.0	37.0	39.0	29.0	39.0
10-11	35.3476015092201	39.0	37.0	39.0	29.0	39.0
12-13	35.30251317568042	39.0	37.0	39.0	29.0	39.0
14-15	36.57844651420446	40.0	38.0	41.0	28.5	41.0
16-17	36.53423985673642	40.0	38.0	41.0	28.0	41.0
18-19	36.487927132647734	40.0	38.0	41.0	27.0	41.0
20-21	36.556527785400746	40.0	38.0	41.0	28.0	41.0
22-23	36.476370251617524	40.0	38.0	41.0	27.5	41.0
24-25	36.476218040439036	40.0	38.0	41.0	28.0	41.0
26-27	36.45149441381784	40.0	38.0	41.0	28.5	41.0
28-29	36.40437276868624	40.0	38.0	41.0	28.5	41.0
30-31	36.318062967562746	40.0	38.0	41.0	28.0	41.0
32-33	36.22834421042286	40.0	37.0	41.0	28.0	41.0
34-35	36.094810332745766	40.0	37.0	41.0	27.0	41.0
36-37	35.97927598535199	40.0	37.0	41.0	27.0	41.0
38-39	35.85339510703285	40.0	37.0	41.0	26.0	41.0
40-41	35.74760897618358	40.0	36.0	41.0	26.0	41.0
42-43	35.47807552737822	40.0	35.5	41.0	24.5	41.0
44-45	35.10235228495464	40.0	35.0	41.0	24.0	41.0
46-47	34.84107302179716	39.0	35.0	41.0	23.0	41.0
48-49	34.63892030260986	39.0	35.0	41.0	23.0	41.0
50-51	34.43648549813581	39.0	34.5	40.0	22.0	41.0
52-53	34.23301393450261	38.5	34.0	40.0	21.5	41.0
54-55	33.99194748618612	38.0	34.0	40.0	20.0	41.0
56-57	33.73699822962763	38.0	34.0	40.0	19.0	41.0
58-59	33.7485292634766	38.0	34.0	40.0	19.5	41.0
60-61	33.53852985062244	37.0	34.0	40.0	18.0	41.0
62-63	33.23025881644162	37.0	33.0	40.0	16.5	41.0
64-65	32.93282796243798	36.0	33.0	39.5	14.0	41.0
66-67	32.612906550633035	36.0	33.0	39.0	12.5	41.0
68-69	32.28469904307991	35.0	33.0	39.0	9.5	40.5
70-71	31.91612302492434	35.0	32.0	38.0	7.5	40.0
72-73	31.574214596701005	35.0	32.0	37.0	7.0	39.5
74-75	31.20619923900793	35.0	32.0	37.0	3.5	39.0
76-77	30.90993693287774	35.0	32.0	36.0	2.0	39.0
78-79	30.63351154060688	35.0	31.5	36.0	2.0	37.5
80-81	30.3751632201631	35.0	31.0	35.0	2.0	37.0
82-83	30.15334302974915	35.0	31.0	35.0	2.0	36.5
84-85	29.94019919561019	35.0	31.0	35.0	2.0	36.0
86-87	29.769652473479518	34.5	31.0	35.0	2.0	36.0
88-89	29.605327263606785	34.0	31.0	35.0	2.0	35.5
90-91	29.420810618660262	34.0	30.5	35.0	2.0	35.0
92-93	29.351830554814534	34.0	30.0	35.0	2.0	35.0
94-95	29.233049354713952	34.0	30.0	35.0	2.0	35.0
96-97	29.147641333025295	34.0	30.0	35.0	2.0	35.0
98-99	29.05877712837178	34.0	30.0	35.0	2.0	35.0
100-101	28.950783775883878	34.0	30.0	35.0	2.0	35.0
102-103	28.060143518867164	33.0	28.0	34.5	2.0	35.0
104-105	29.002128722791852	34.0	30.5	35.0	2.0	35.0
106-107	29.298830431003342	34.5	31.0	35.0	2.0	35.0
108-109	29.310933612950905	35.0	31.0	35.0	2.0	35.0
110-111	29.256689952530536	35.0	31.0	35.0	2.0	35.0
112-113	29.18894161855687	35.0	31.0	35.0	2.0	35.0
114-115	29.084044184001296	34.5	31.0	35.0	2.0	35.0
116-117	28.973870414359034	34.0	31.0	35.0	2.0	35.0
118-119	28.695812820457185	34.0	30.0	35.0	2.0	35.0
120-121	28.58731401198033	34.0	30.0	35.0	2.0	35.0
122-123	28.346609743302388	34.0	30.0	35.0	2.0	35.0
124-125	27.56811753383428	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65999.0
3	10347.0
4	5671.0
5	4994.0
6	5379.0
7	5870.0
8	5811.0
9	6048.0
10	6738.0
11	7026.0
12	6919.0
13	6884.0
14	6831.0
15	6196.0
16	5544.0
17	5028.0
18	4520.0
19	4309.0
20	4055.0
21	4343.0
22	4960.0
23	6681.0
24	9679.0
25	11926.0
26	13080.0
27	14975.0
28	17756.0
29	21264.0
30	25936.0
31	32981.0
32	43451.0
33	61658.0
34	95127.0
35	160896.0
36	317661.0
37	444365.0
38	105941.0
39	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.70710872398909	18.014572111781657	12.361591110169199	24.91672805406006
2	16.730407875656134	19.461982496272544	38.75884632666817	25.04876330140316
3	20.28702270587672	23.8904118332243	27.924877535064063	27.897687925834912
4	13.501698738344512	16.068411636565095	34.51646113517719	35.9134284899132
5	15.241675789016798	37.40167808689158	32.52143885259453	14.835207271497097
6	34.18939738473252	34.04037874878	17.159562997899812	14.610660868587663
7	30.004833752748116	31.535479553608997	19.62420605451477	18.83548063912811
8	27.226146458322827	34.614571767336464	18.82730927797532	19.331972496365392
9	27.703732264132995	16.304517822523554	18.35618746923932	37.63556244410413
10-11	26.359578705948795	25.66508393541077	26.256397355467094	21.718940003173337
12-13	26.328936257398404	23.305587522491212	26.44344277833414	23.922033441776243
14-15	24.101471682840668	24.46434932263266	23.707090812291813	27.72708818223486
16-17	24.525284535613874	27.38561119296839	24.375419749082617	23.71368452233512
18-19	24.492912538765164	26.668262761979094	25.672522740367963	23.16630195888778
20-21	25.24803149983291	25.553843654373853	25.54225720075749	23.655867645035748
22-23	25.3551047039363	25.121028314528697	25.181773898742886	24.34209308279211
24-25	24.737099808550315	25.627190834928747	25.262102218428833	24.373607138092105
26-27	24.43768806779475	25.81680401109141	25.454411376932395	24.291096544181446
28-29	24.5085548596717	25.82377854187024	25.494972621320606	24.172693977137456
30-31	24.98002755766155	25.498816335558733	25.388477169495577	24.132678937284137
32-33	24.407480678127964	25.720129538094426	25.391432688004834	24.480957095772776
34-35	24.44823265576492	26.004708661543003	25.43312503491592	24.113933647776157
36-37	24.722103435730837	25.728792356248842	25.35515742578681	24.193946782233514
38-39	24.710949912453554	25.8808742771753	25.34665903228463	24.061516778086514
40-41	24.63118754832214	25.777830839469303	25.406480577611983	24.18450103459658
42-43	24.670079599639983	25.5433457381957	25.387274270868577	24.399300391295746
44-45	24.538271374835876	25.647664755816006	25.462335008013824	24.351728861334283
46-47	24.491178101149348	25.651728330007096	25.550488977772982	24.306604591070574
48-49	24.450406782766287	25.83955500783754	25.446130049834043	24.26390815956213
50-51	24.33456046870741	25.89508854333903	25.466240158925412	24.304110829028147
52-53	24.523984078597167	25.77119893423245	25.348141050785777	24.356675936384605
54-55	24.39800739057915	25.62743004068074	25.42977130568388	24.544791263056233
56-57	24.396890487875254	25.777218444631124	25.302480358086697	24.523410709406924
58-59	24.45085299462486	25.66748816109716	25.46536989270785	24.416288951570124
60-61	24.554659016884226	25.77930084026962	25.4671240960806	24.19891604676555
62-63	24.37060830133181	25.801261592041836	25.514779853337693	24.313350253288668
64-65	24.30932649399377	25.630895244101488	25.45264559501552	24.607132666889225
66-67	24.37100986327009	25.744469106343427	25.318652375106844	24.565868655279637
68-69	24.383004283845796	25.782642860288924	25.40503758817758	24.429315267687702
70-71	24.307480727705496	25.647347338016317	25.555694600692867	24.489477333585317
72-73	24.447238161623456	25.835617723741798	25.29301316440813	24.42413095022662
74-75	24.344544905646888	25.820731005237512	25.3334558781292	24.501268210986396
76-77	24.343957773300527	25.616196986442407	25.44456086141037	24.595284378846692
78-79	24.32814312775183	25.60676253413104	25.52128931153841	24.543805026578724
80-81	24.299772029562433	25.95531038990509	25.304878146083	24.440039434449485
82-83	24.39880004174767	25.809411673835797	25.40195696989795	24.38983131451858
84-85	24.326999884153953	25.850678592958793	25.230375663521805	24.591945859365453
86-87	24.279188551145133	25.805625937307813	25.361973555752083	24.553211955794975
88-89	24.292983970218003	25.676599531209305	25.408436445157108	24.621980053415584
90-91	24.39222424924072	25.625297616387954	25.41200321202287	24.570474922348453
92-93	24.320772581796575	25.77842651751326	25.487674388250948	24.41312651243922
94-95	24.28010987078662	25.613762761692282	25.5501278283478	24.5559995391733
96-97	24.34166077047224	25.578600979071464	25.503793510962776	24.575944739493526
98-99	24.192864329979024	25.661503564133177	25.350038821399544	24.795593284488255
100-101	24.143732609374847	25.741109100233505	25.495155118233114	24.620003172158533
102-103	24.03249472345276	25.63004239746394	25.58961067474067	24.747852204342628
104-105	24.016775229539313	25.728603030059343	25.519391333095914	24.735230407305433
106-107	23.971186225959137	25.73340366375548	25.563321472254426	24.73208863803095
108-109	23.995389037218974	25.709723808180375	25.50247303091017	24.79241412369048
110-111	23.832089140259075	26.017324885967586	25.519424282283442	24.631161691489897
112-113	23.763780316536824	26.004945548862423	25.584966399434283	24.646307735166463
114-115	23.68064373958214	26.12243217600076	25.54546263995397	24.651461444463134
116-117	23.708427353356686	26.02094496465932	25.561000765563126	24.709626916420866
118-119	23.74964601076127	25.843310363364953	25.54072756188212	24.866316063991654
120-121	23.669160571247318	26.004619350342427	25.627023518607277	24.69919655980297
122-123	23.577555135544106	26.10508022746992	25.55433906113932	24.763025575846655
124-125	23.475679437333504	26.060809569325304	25.65347687662733	24.81003411671386
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	756.0
1	567.0
2	321.5
3	222.5
4	165.5
5	143.5
6	145.0
7	178.5
8	204.5
9	212.0
10	217.5
11	214.5
12	233.0
13	252.0
14	266.0
15	321.5
16	368.0
17	399.5
18	460.5
19	538.0
20	596.0
21	682.5
22	817.5
23	1006.5
24	1242.5
25	1577.5
26	2130.0
27	2879.5
28	3776.0
29	4931.5
30	6675.0
31	9002.5
32	11888.5
33	14917.5
34	17957.0
35	21141.5
36	24711.0
37	28375.5
38	31082.5
39	33834.0
40	36831.0
41	39740.0
42	42604.5
43	46313.0
44	50725.5
45	54297.5
46	57576.0
47	60951.0
48	64864.5
49	68261.5
50	70682.0
51	70843.0
52	68406.5
53	66624.0
54	65854.0
55	64050.0
56	60234.5
57	54961.5
58	50186.5
59	45709.5
60	40009.0
61	34451.5
62	29069.5
63	22679.5
64	16809.5
65	12997.5
66	10134.0
67	7767.0
68	6097.5
69	4860.5
70	3936.0
71	3045.5
72	2407.0
73	2010.0
74	1575.0
75	1212.0
76	901.0
77	590.0
78	396.0
79	285.0
80	191.5
81	136.5
82	111.0
83	92.0
84	59.0
85	46.5
86	45.0
87	36.0
88	31.5
89	29.5
90	25.0
91	25.0
92	22.5
93	13.5
94	11.5
95	10.0
96	9.0
97	7.0
98	4.5
99	4.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022209429817563575
2	0.009573030093777402
3	0.008105165479398201
4	0.009636850294402586
5	0.015125387548168296
6	0.08462558602899224
7	0.053034586719526815
8	0.04524852224325452
9	0.023804934833193143
10-11	0.0464291959548204
12-13	0.0114238159119077
14-15	0.025272799447572346
16-17	0.031271898306339516
18-19	0.03698380626229337
20-21	0.02664493376101377
22-23	0.034016166933222375
24-25	0.027634146870704104
26-27	0.04116402940324283
28-29	0.04011099609292732
30-31	0.02351774393037982
32-33	0.026357742858200448
34-35	0.04081301829980433
36-37	0.008424266482524114
38-39	0.01946516119068072
40-41	0.03771773856948297
42-43	0.019911902595056995
44-45	0.014997747146917931
46-47	0.0202629136984955
48-49	0.02568763075163603
50-51	0.024315496438194605
52-53	0.02115639650724806
54-55	0.01997572279568218
56-57	0.022592351021314672
58-59	0.015635949153169758
60-61	0.020518194500996234
62-63	0.019784262193806634
64-65	0.01780583597442597
66-67	0.02444313683944497
68-69	0.020358643999433275
70-71	0.0423766132151213
72-73	0.018603588482240755
74-75	0.008168985680023383
76-77	0.01375325323472687
78-79	0.021028756105997692
80-81	0.016050780457233446
82-83	0.022177519717250984
84-85	0.0106898836047181
86-87	0.015795499654732714
88-89	0.020294823798808096
90-91	0.018380217780052614
92-93	0.006605390764706408
94-95	0.00973258059534036
96-97	0.013625612833476504
98-99	0.007115952369707869
100-101	0.00928583919096408
102-103	0.004020672639386509
104-105	0.0043397736425124225
106-107	7.977525078147835E-4
108-109	0.01282786032566172
110-111	0.0014678646143792017
112-113	0.0024570777240695333
114-115	0.011583366413470658
116-117	0.005201346350952389
118-119	0.277905063622358
120-121	9.573030093777403E-4
122-123	8.615727084399662E-4
124-125	0.31342100527027217
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1566902.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.56121995372247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.62247816556071	54.16830464133958
2	9.798027663026538	12.847412934566806
3	3.057342054165088	6.013292246606491
4	1.3579008165204942	3.561025364289579
5	0.8253854799662638	2.705663949933851
6	0.48468791670249783	1.9066038669506389
7	0.3517636766095613	1.614343904175069
8	0.26483417230431083	1.3890281137363967
9	0.19020618651698465	1.1223134667718915
>10	0.9941494264321756	11.440831964265694
>50	0.041845285667940435	1.840244842550802
>100	0.010990169643590716	1.143497138526237
>500	1.9449344195147285E-4	0.09971459769977586
>1k	1.9449344195147285E-4	0.1477229685871999
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.382020062518268E-5	6.382020062518268E-5
10-11	0.0	0.0	0.0	6.382020062518268E-5	6.382020062518268E-5
12-13	6.382020062518268E-5	0.0	0.0	6.382020062518268E-5	1.595505015629567E-4
14-15	6.382020062518268E-5	0.0	0.0	6.382020062518268E-5	4.4674140437627884E-4
16-17	6.382020062518268E-5	0.0	0.0	6.382020062518268E-5	7.020222068770095E-4
18-19	6.382020062518268E-5	0.0	0.0	6.382020062518268E-5	7.020222068770095E-4
20-21	6.382020062518268E-5	0.0	0.0	1.2764040125036536E-4	7.658424075021922E-4
22-23	6.382020062518268E-5	0.0	0.0	3.5101110343850475E-4	8.29662608127375E-4
24-25	6.382020062518268E-5	0.0	0.0	6.382020062518268E-4	8.615727084399663E-4
26-27	6.382020062518268E-5	0.0	0.0	0.001021123210002923	8.934828087525576E-4
28-29	6.382020062518268E-5	0.0	0.0	0.0031910100312591344	8.934828087525576E-4
30-31	6.382020062518268E-5	0.0	0.0	0.01094516440721883	8.934828087525576E-4
32-33	6.382020062518268E-5	0.0	0.0	0.026198192356637492	9.573030093777403E-4
34-35	6.382020062518268E-5	0.0	0.0	0.0445784101366901	0.001021123210002923
36-37	6.382020062518268E-5	0.0	0.0	0.0722444671077068	0.0010530333103155143
38-39	9.573030093777403E-5	0.0	0.0	0.1161208550375199	0.0011487636112532883
40-41	1.2764040125036536E-4	0.0	0.0	0.1661878024279757	0.0011487636112532883
42-43	1.2764040125036536E-4	0.0	0.0	0.2159675589156182	0.0012444939121910624
44-45	1.2764040125036536E-4	0.0	0.0	0.27346955967890785	0.0013083141128162452
46-47	1.2764040125036536E-4	0.0	0.0	0.34044886023503707	0.0013402242131288363
48-49	1.2764040125036536E-4	0.0	0.0	0.40573692547459894	0.0013402242131288363
50-51	1.2764040125036536E-4	0.0	0.0	0.46978049680196976	0.0013402242131288363
52-53	1.2764040125036536E-4	0.0	0.0	0.5410995710006115	0.001499774714691793
54-55	1.2764040125036536E-4	0.0	0.0	0.6180348228542691	0.0015316848150043845
56-57	1.2764040125036536E-4	0.0	0.0	0.7065215310210848	0.0015316848150043845
58-59	1.2764040125036536E-4	0.0	0.0	0.7995394734322887	0.0015316848150043845
60-61	1.2764040125036536E-4	0.0	0.0	0.8950144935675619	0.0015955050156295672
62-63	1.2764040125036536E-4	0.0	0.0	0.9896917611950204	0.00165932521625475
64-65	1.2764040125036536E-4	0.0	0.0	1.0962076760384503	0.00165932521625475
66-67	1.2764040125036536E-4	0.0	0.0	1.2131901037844104	0.00165932521625475
68-69	1.2764040125036536E-4	0.0	0.0	1.3436705039625962	0.00165932521625475
70-71	1.2764040125036536E-4	0.0	0.0	1.4818093282158042	0.00165932521625475
72-73	1.2764040125036536E-4	0.0	0.0	1.6214160170833911	0.0018507858181302979
74-75	1.2764040125036536E-4	0.0	0.0	1.7709148370478818	0.0019146060187554806
76-77	1.2764040125036536E-4	0.0	0.0	1.9427188171308734	0.0019146060187554806
78-79	1.2764040125036536E-4	0.0	0.0	2.133828407902983	0.0019146060187554806
80-81	1.2764040125036536E-4	0.0	0.0	2.3198962028257033	0.001978426219380663
82-83	1.595505015629567E-4	0.0	0.0	2.515824218745014	0.002042246420005846
84-85	1.9146060187554806E-4	0.0	0.0	2.7233994212784207	0.002042246420005846
86-87	1.9146060187554806E-4	0.0	0.0	2.957651467673154	0.0021060666206310285
88-89	1.9146060187554806E-4	0.0	0.0	3.2195057508382785	0.0021698868212562113
90-91	2.233707021881394E-4	0.0	0.0	3.4984957578712645	0.0021698868212562113
92-93	2.552808025007307E-4	0.0	0.0	3.7697316105282908	0.002233707021881394
94-95	2.552808025007307E-4	0.0	0.0	4.056794872940363	0.0023294373228191683
96-97	2.552808025007307E-4	0.0	0.0	4.374779022555336	0.002425167623756942
98-99	2.552808025007307E-4	0.0	0.0	4.729587427931039	0.002488987824382125
100-101	2.552808025007307E-4	0.0	0.0	5.1118066094752574	0.002488987824382125
102-103	2.552808025007307E-4	0.0	0.0	5.506119719037949	0.0025528080250073076
104-105	2.552808025007307E-4	0.0	0.0	5.9052831638481535	0.0026166282256324903
106-107	2.552808025007307E-4	0.0	0.0	6.341494235121278	0.0026485383259450814
108-109	2.552808025007307E-4	0.0	0.0	6.813540349045441	0.0026804484262576726
110-111	2.552808025007307E-4	0.0	0.0	7.315518137062815	0.002839998927820629
112-113	2.552808025007307E-4	0.0	0.0	7.827324235976468	0.0028719090281332208
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4120	0.0	36.982773	1
GTACATG	9710	0.0	31.138756	1
TCAACGC	4820	0.0	30.617117	4
CAACGCA	4940	0.0	30.109488	5
CATGGGG	7420	0.0	29.993513	4
TACATGG	10090	0.0	29.546509	2
ATCAACG	5000	0.0	29.276875	3
ATGGGGG	4095	0.0	28.622171	5
ACATGGG	10490	0.0	28.363129	3
AACGCAG	5255	0.0	28.301924	6
TATCAAC	5270	0.0	28.228577	2
GGTATCA	2775	0.0	27.882849	1
ACGCAGA	6030	0.0	24.659723	7
CGCAGAG	6355	0.0	23.211414	8
GAGTCCG	585	0.0	21.361074	3
TATAACG	195	4.0371193E-5	21.361074	2
GTATATA	425	1.8189894E-12	21.00676	1
GCAGAGT	7155	0.0	20.201143	9
GAGTACA	7615	0.0	19.22746	1
AGTACAT	7490	0.0	18.987846	2
>>END_MODULE