FastQCFastQC Report
Mon 15 Aug 2016
SRR2921611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566902
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28960.18482330101052907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.13944713836602415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59350.040.1994821
GGTATCA40600.037.903111
TCAACGC72600.032.198334
CAACGCA74400.031.6183195
TATCAAC76350.031.0093862
ATCAACG75800.030.8410073
AACGCAG77000.030.6436986
GTACATG101350.029.2491861
TACATGG104150.028.171992
CATGGGG75100.027.0768644
ACATGGG105450.027.032923
ACGCAGA88700.026.600787
CGCAGAG91300.025.9721158
GAGTACT38100.025.46812212-13
GTACTTT39450.024.43632514-15
ATGGGGG39900.024.3292835
GCAGAGT100100.023.5095449
TATCACG2254.9776554E-621.2040672
AGAGTAC91800.021.092210-11
GTACTAG3451.2460077E-920.7464281
Produced by FastQC (version 0.11.5)