##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921611_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1566902
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72190092296774	33.0	31.0	34.0	30.0	34.0
2	31.906415334207246	33.0	31.0	34.0	30.0	34.0
3	31.96805033116302	34.0	31.0	34.0	30.0	34.0
4	35.371880309042936	37.0	35.0	37.0	33.0	37.0
5	35.24060470916496	37.0	35.0	37.0	33.0	37.0
6	35.2634344713326	37.0	35.0	37.0	33.0	37.0
7	35.153246980347205	37.0	35.0	37.0	33.0	37.0
8	35.19047840898793	37.0	35.0	37.0	33.0	37.0
9	36.90570820638432	39.0	38.0	39.0	34.0	39.0
10-11	36.85556148374308	39.0	38.0	39.0	33.5	39.0
12-13	36.797600615737295	39.0	38.0	39.0	33.0	39.0
14-15	38.21060538565909	41.0	39.0	41.0	34.0	41.0
16-17	38.21608498808477	41.0	39.0	41.0	34.0	41.0
18-19	38.2322685145593	41.0	39.0	41.0	34.0	41.0
20-21	38.171822168840166	41.0	39.0	41.0	34.0	41.0
22-23	38.17158986330989	41.0	39.0	41.0	34.0	41.0
24-25	38.131259325726816	41.0	39.0	41.0	34.0	41.0
26-27	38.053367409065785	41.0	39.0	41.0	33.0	41.0
28-29	37.9833904736863	41.0	39.0	41.0	33.0	41.0
30-31	37.863349781926374	41.0	38.0	41.0	33.0	41.0
32-33	37.73432543962545	41.0	38.0	41.0	33.0	41.0
34-35	37.602837318479395	40.5	38.0	41.0	33.0	41.0
36-37	37.51585612884533	40.0	38.0	41.0	32.5	41.0
38-39	37.42970141080936	40.0	38.0	41.0	32.0	41.0
40-41	37.30646013598809	40.0	38.0	41.0	32.0	41.0
42-43	37.13220960851413	40.0	37.5	41.0	31.5	41.0
44-45	36.958951485159886	40.0	37.0	41.0	31.0	41.0
46-47	36.730158618726634	40.0	37.0	41.0	30.5	41.0
48-49	36.51974341726541	40.0	36.5	41.0	30.0	41.0
50-51	36.270968126915406	40.0	36.0	41.0	29.5	41.0
52-53	36.14614219651261	40.0	36.0	41.0	29.0	41.0
54-55	36.01931263091119	40.0	35.0	41.0	29.0	41.0
56-57	35.823162201592694	39.0	35.0	41.0	29.0	41.0
58-59	35.63191795019726	39.0	35.0	41.0	28.5	41.0
60-61	35.38204846250755	39.0	35.0	41.0	28.0	41.0
62-63	35.03798578341211	38.0	35.0	41.0	28.0	41.0
64-65	34.75340416950135	37.0	35.0	40.0	28.0	41.0
66-67	34.45096279154663	37.0	35.0	39.5	28.0	41.0
68-69	34.08711106374234	36.0	35.0	39.0	27.5	41.0
70-71	33.71610445324596	35.5	34.5	39.0	26.0	41.0
72-73	33.39677656930682	35.0	34.0	37.5	26.0	40.0
74-75	33.07796882000278	35.0	34.0	37.0	26.0	39.0
76-77	32.75289616070437	35.0	34.0	37.0	26.0	39.0
78-79	32.48612165917205	35.0	34.0	36.0	26.0	38.5
80-81	32.23042506806425	35.0	34.0	36.0	26.0	37.0
82-83	32.0063899975876	35.0	34.0	35.0	25.0	37.0
84-85	31.794171556357703	35.0	34.0	35.0	24.5	36.5
86-87	31.664783119812213	35.0	33.5	35.0	24.5	36.0
88-89	31.565499948305636	35.0	33.0	35.0	24.0	36.0
90-91	31.457425863263943	35.0	33.0	35.0	24.0	36.0
92-93	31.380367757524084	35.0	33.0	35.0	24.0	35.0
94-95	31.309329173107187	35.0	33.0	35.0	24.0	35.0
96-97	31.24813900294977	35.0	33.0	35.0	23.0	35.0
98-99	31.168435549893996	35.0	33.0	35.0	23.0	35.0
100-101	31.09757725754387	35.0	33.0	35.0	21.5	35.0
102-103	30.400954877841755	34.0	31.5	35.0	20.0	35.0
104-105	30.924762365482973	35.0	33.0	35.0	20.0	35.0
106-107	31.023777492146927	35.0	33.0	35.0	20.0	35.0
108-109	31.0130866512392	35.0	33.0	35.0	20.0	35.0
110-111	30.944494295112264	35.0	33.0	35.0	19.0	35.0
112-113	30.910798505586182	35.0	33.0	35.0	19.0	35.0
114-115	30.791894451599397	35.0	33.0	35.0	18.0	35.0
116-117	30.976443006646235	35.0	33.0	35.0	19.0	35.0
118-119	30.959925062320426	35.0	33.0	35.0	18.5	35.0
120-121	30.881334952664556	35.0	33.0	35.0	16.5	35.0
122-123	30.748698067907245	35.0	33.0	35.0	2.0	35.0
124-125	30.10050532834855	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24657.0
3	6958.0
4	3655.0
5	2918.0
6	3057.0
7	3438.0
8	3865.0
9	4185.0
10	4508.0
11	4943.0
12	5582.0
13	7426.0
14	10203.0
15	9157.0
16	5021.0
17	4109.0
18	3666.0
19	3426.0
20	3265.0
21	3423.0
22	3767.0
23	4465.0
24	5986.0
25	7659.0
26	9288.0
27	11104.0
28	13343.0
29	16357.0
30	19325.0
31	24542.0
32	31881.0
33	43326.0
34	66813.0
35	120365.0
36	280603.0
37	577553.0
38	212798.0
39	265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.283678318232134	18.2072999000346	11.625542117606805	26.883479664126465
2	15.584320267740729	19.81544321263344	37.787488456425436	26.81274806320039
3	18.57629667837882	23.986698704853733	28.354561611374407	29.08244300539304
4	13.171157671871084	15.421220319220208	34.385595712364285	37.022026296544425
5	14.531320516724215	37.35207246956869	32.773253004511155	15.34335400919594
6	34.234732453944474	34.31545095074128	16.58318092182567	14.86663567348857
7	30.284037761027733	31.504726513489956	19.475853438175417	18.735382287306894
8	28.092484515790034	34.27761316194559	18.592224982490283	19.0376773397741
9	27.861411459626755	15.39439269519497	18.56767752730588	38.1765183178724
10-11	26.76377150775381	25.15212275822874	26.144265184338494	21.93984054967896
12-13	26.847889004856484	22.622195133554122	26.741857941362685	23.78805792022671
14-15	24.240424471541676	23.807339112700205	24.356158671748116	27.596077744009996
16-17	24.22280814161925	26.87310907710033	24.98331151299691	23.92077126828351
18-19	24.136845737480062	25.784358508074128	27.0115102131236	23.067285541322207
20-21	24.984480390714342	24.831853611065984	26.84006711492937	23.343598883290305
22-23	24.75101460948305	24.44645625420028	26.63216664677549	24.170362489541176
24-25	24.236204385990945	24.918888749541672	26.69384256551207	24.151064298955312
26-27	23.977472026713436	25.09496748895237	26.872608261660965	24.054952222673233
28-29	24.008590566332767	25.131140164726983	26.875005983399657	23.985263285540594
30-31	24.455186340120978	24.950027634781787	26.65508728889388	23.93969873620335
32-33	24.03200842494934	25.018589140112653	26.692473392797307	24.256929042140705
34-35	24.18332898805138	25.169266549381287	26.759990771195536	23.8874136913718
36-37	24.367194946941677	25.00970919640404	26.623430293394847	23.99966556325944
38-39	24.300809482371015	25.15467983691006	26.678933194967914	23.86557748575101
40-41	24.312593958389776	25.008671854781294	26.660903513130318	24.017830673698608
42-43	24.28826718660748	24.91296203517259	26.494994718686275	24.30377605953365
44-45	24.29630849363347	25.027244560900648	26.37854286993487	24.29790407553102
46-47	24.294588866916065	25.086535942372983	26.311138796088546	24.307736394622406
48-49	24.297539965861844	25.368082899107357	26.063642226517413	24.270734908513383
50-51	24.297752315988564	25.448957625650216	25.97966212462413	24.27362793373709
52-53	24.454380623005203	25.380323685979032	25.78389407290497	24.381401618110793
54-55	24.3010701903979	25.288066815417686	25.83382051845006	24.577042475734352
56-57	24.28714796962778	25.426441188530802	25.807194813939056	24.47921602790236
58-59	24.32792932508133	25.280004850364655	25.972298673967742	24.41976715058627
60-61	24.401493394600806	25.30560342076712	26.08625311123875	24.206650073393323
62-63	24.22472846629287	25.3726418601475	26.076394174051536	24.3262354995081
64-65	24.172571098894508	25.13316084860444	26.092378464000937	24.60188958850011
66-67	24.25488121849765	25.294165513874194	25.913530193589356	24.537423074038795
68-69	24.243079853914676	25.31092592503953	25.94734482423012	24.498649396815672
70-71	24.106644831648694	25.194045320000868	26.112035085793494	24.587274762556945
72-73	24.362341363167854	25.281501597138234	25.849323330344347	24.50683370934956
74-75	24.135942604876238	25.3966785016559	25.89654351512583	24.570835378342036
76-77	24.133993925496387	25.166621247232552	25.93949620806228	24.759888619208784
78-79	24.066883655625755	25.25962090752441	25.992086285021383	24.68140915182845
80-81	24.028241715180656	25.519304972487113	25.806495875300435	24.6459574370318
82-83	24.154023287814617	25.34640049833166	25.83777542908347	24.66180078477025
84-85	24.035141295617485	25.389653096020133	25.687156182578757	24.888049425783624
86-87	23.891028283836512	25.460143646507564	25.82985406872925	24.81897400092667
88-89	23.92341367636627	25.252100424695996	25.77785677383574	25.046629125101994
90-91	23.89525765168957	25.292673236996155	25.829819212230575	24.982249899083698
92-93	23.89469794537246	25.32650414639843	25.96036637900775	24.818431529221353
94-95	23.85081211968039	25.16174901785837	26.00231737877719	24.98512148368405
96-97	23.914482228685177	25.099481197973805	25.906052732853013	25.079983840488
98-99	23.785434499924214	25.17360052971257	25.774664741406134	25.26630022895709
100-101	23.665104773623366	25.224040814294703	26.00976321429164	25.101091197790286
102-103	23.72664147860717	25.0184442969468	25.9965345144491	25.258379709996937
104-105	23.687633304444056	25.164368926710157	25.893067977448492	25.25492979139729
106-107	23.670460579387527	25.085583216754653	25.92613038772197	25.31782581613585
108-109	23.67931220556226	25.05521380771203	25.83524346568798	25.43023052103773
110-111	23.552197475206626	25.295671295018412	25.797458102795932	25.354673126979026
112-113	23.42819069415242	25.273781644160927	25.868013618002212	25.43001404368444
114-115	23.314093670184864	25.347564812604745	25.88145270093471	25.45688881627568
116-117	23.253862948688248	25.27693693107227	25.87708129503795	25.59211882520153
118-119	23.3255364231648	25.089542141608057	25.866350535465838	25.718570899761307
120-121	23.143673517319375	25.188976920690965	25.97609355793569	25.691256004053976
122-123	22.99948400956162	25.263459867463535	25.86158755930141	25.875468563673444
124-125	22.92977476588288	25.234021294931676	25.883493074068465	25.95271086511698
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	43.5
2	36.0
3	63.5
4	67.5
5	63.5
6	138.0
7	390.5
8	705.5
9	776.5
10	625.5
11	496.5
12	499.5
13	553.5
14	584.5
15	660.0
16	754.0
17	836.5
18	895.0
19	948.5
20	1010.0
21	1069.0
22	1208.0
23	1371.0
24	1562.0
25	1879.0
26	2408.0
27	3139.5
28	3916.5
29	5071.5
30	6760.0
31	8688.5
32	11604.0
33	14703.0
34	17453.0
35	20532.0
36	24067.5
37	27661.0
38	30559.0
39	33532.0
40	36472.0
41	39357.0
42	42324.0
43	46107.0
44	50333.5
45	53988.0
46	57713.0
47	61308.5
48	64969.0
49	68693.5
50	71545.0
51	71629.5
52	69124.0
53	67247.0
54	66744.5
55	64678.0
56	60351.0
57	54960.5
58	50258.0
59	45828.0
60	40005.0
61	34314.5
62	28933.0
63	22468.5
64	16469.0
65	12509.5
66	9494.5
67	7242.5
68	5624.5
69	4324.0
70	3467.0
71	2791.5
72	2164.5
73	1776.5
74	1452.5
75	1071.5
76	722.5
77	438.5
78	261.5
79	154.0
80	94.5
81	51.5
82	25.0
83	15.5
84	10.5
85	7.5
86	5.5
87	4.5
88	2.5
89	2.5
90	2.0
91	1.0
92	2.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022975272225065766
2	8.296626081273748E-4
3	0.027953247873830016
4	0.16529431961922317
5	0.021762688413187296
6	0.06165031380392647
7	0.04703548786075964
8	0.04014290619323991
9	0.030697516500712868
10-11	0.057246719960788874
12-13	0.05434290083234306
14-15	0.05191773320858611
16-17	0.04483369093919084
18-19	0.03258021241915576
20-21	0.021411677309748793
22-23	0.0027442686268828553
24-25	0.0046269645453257445
26-27	0.003191010031259134
28-29	0.004275953441887239
30-31	0.0022975272225065767
32-33	0.008105165479398201
34-35	0.004371683742825014
36-37	0.005648087755328668
38-39	0.002329437322819168
40-41	0.003286740332196908
42-43	0.0036377514356354134
44-45	0.004818425147201293
46-47	0.004307863542199831
48-49	0.0021379767209436197
50-51	0.0014359545140666104
52-53	8.296626081273748E-4
54-55	0.004977975648764249
56-57	6.062919059392354E-4
58-59	6.062919059392354E-4
60-61	1.2764040125036536E-4
62-63	9.892131096903315E-4
64-65	0.0
66-67	0.0034782009340724564
68-69	6.062919059392354E-4
70-71	0.0
72-73	0.0023613474231317594
74-75	0.005041795849389432
76-77	0.001595505015629567
78-79	1.2764040125036536E-4
80-81	0.0
82-83	0.004722694846263519
84-85	0.001755055517192524
86-87	0.0
88-89	0.0010530333103155143
90-91	6.062919059392354E-4
92-93	0.0
94-95	0.002903819128445812
96-97	0.0014678646143792017
98-99	9.253929090651489E-4
100-101	0.0
102-103	0.001404044413754019
104-105	0.0
106-107	3.8292120375109606E-4
108-109	0.0024570777240695333
110-111	9.573030093777402E-5
112-113	3.5101110343850475E-4
114-115	0.0
116-117	0.0018507858181302979
118-119	0.0026804484262576726
120-121	0.002808088827508038
122-123	7.977525078147835E-4
124-125	0.09911277157090871
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1566902.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.05243554962654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.19729338188957	49.76437378834193
2	11.142662530730162	13.828586970787438
3	3.456523738773713	6.434571495780299
4	1.623158148320267	4.028836655419781
5	0.8928922786665662	2.7703070287358136
6	0.5684012201642316	2.1162408048342036
7	0.40138457353267	1.7434823265825234
8	0.3000441844568921	1.4894777934441246
9	0.21193058325341918	1.18357279724948
>10	1.1394164164858485	12.430094721044268
>50	0.05091790661973545	2.118862587463087
>100	0.014652789874619081	1.4273381040637887
>500	2.063563521335948E-4	0.08444840941956602
>1k	5.158908803339871E-4	0.5798065168336541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2896	0.18482330101052907	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2185	0.13944713836602415	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2764040125036536E-4
2	0.0	0.0	0.0	0.0	1.2764040125036536E-4
3	0.0	0.0	0.0	0.0	1.2764040125036536E-4
4	0.0	0.0	0.0	0.0	1.2764040125036536E-4
5	0.0	0.0	0.0	0.0	1.2764040125036536E-4
6	0.0	0.0	0.0	0.0	1.9146060187554806E-4
7	0.0	0.0	0.0	0.0	1.9146060187554806E-4
8	0.0	0.0	0.0	0.0	1.9146060187554806E-4
9	0.0	0.0	0.0	0.0	1.9146060187554806E-4
10-11	0.0	0.0	0.0	0.0	1.9146060187554806E-4
12-13	0.0	0.0	0.0	0.0	3.5101110343850475E-4
14-15	0.0	0.0	0.0	0.0	4.7865150468887014E-4
16-17	0.0	0.0	0.0	0.0	7.658424075021922E-4
18-19	0.0	0.0	0.0	0.0	7.658424075021922E-4
20-21	0.0	0.0	0.0	6.382020062518268E-5	8.615727084399663E-4
22-23	0.0	0.0	0.0	2.871909028133221E-4	9.892131096903315E-4
24-25	0.0	0.0	0.0	5.743818056266442E-4	0.001021123210002923
26-27	0.0	0.0	0.0	9.573030093777402E-4	0.001021123210002923
28-29	0.0	0.0	0.0	0.0031910100312591344	0.001021123210002923
30-31	0.0	0.0	0.0	0.010913254306906239	0.0010530333103155143
32-33	0.0	0.0	0.0	0.02645347315913822	0.0010849434106281056
34-35	0.0	0.0	0.0	0.04441885963512715	0.001212583811878471
36-37	0.0	0.0	0.0	0.07205300650583124	0.001212583811878471
38-39	0.0	0.0	0.0	0.11586557423501917	0.001212583811878471
40-41	0.0	0.0	0.0	0.1662197125282883	0.0012444939121910624
42-43	0.0	0.0	0.0	0.21590373871499302	0.0012764040125036538
44-45	3.191010031259134E-5	0.0	0.0	0.27388439098297146	0.0012764040125036538
46-47	6.382020062518268E-5	0.0	0.0	0.3410870622412889	0.0012764040125036538
48-49	1.2764040125036536E-4	0.0	0.0	0.4059602961767871	0.0012764040125036538
50-51	1.9146060187554806E-4	0.0	0.0	0.47029105840697116	0.0012764040125036538
52-53	1.9146060187554806E-4	0.0	0.0	0.5416420427059254	0.0013083141128162452
54-55	1.9146060187554806E-4	0.0	0.0	0.6178752723527061	0.0013402242131288363
56-57	1.9146060187554806E-4	0.0	0.0	0.7057875987138953	0.001404044413754019
58-59	1.9146060187554806E-4	0.0	0.0	0.7997947542347894	0.0014678646143792017
60-61	1.9146060187554806E-4	0.0	0.0	0.8958760662760019	0.0014678646143792017
62-63	1.9146060187554806E-4	0.0	0.0	0.9912553561103374	0.0015955050156295672
64-65	2.552808025007307E-4	0.0	0.0	1.0986009335618947	0.0015955050156295672
66-67	2.552808025007307E-4	0.0	0.0	1.2167640350194204	0.0015955050156295672
68-69	2.552808025007307E-4	0.0	0.0	1.3491271311160493	0.0015955050156295672
70-71	2.552808025007307E-4	0.0	0.0	1.4897230330933269	0.0015955050156295672
72-73	2.552808025007307E-4	0.0	0.0	1.6309252269765435	0.0016274151159421585
74-75	2.552808025007307E-4	0.0	0.0	1.782211012558539	0.00165932521625475
76-77	2.871909028133221E-4	0.0	0.0	1.954876565349971	0.00165932521625475
78-79	3.1910100312591345E-4	0.0	0.0	2.1483155934448996	0.00165932521625475
80-81	3.1910100312591345E-4	0.0	0.0	2.337542488298566	0.00165932521625475
82-83	3.1910100312591345E-4	0.0	0.0	2.536757244550074	0.0017231454168799324
84-85	3.1910100312591345E-4	0.0	0.0	2.7476510975159902	0.0017231454168799324
86-87	3.1910100312591345E-4	0.0	0.0	2.9847750529388564	0.0017550555171925238
88-89	3.1910100312591345E-4	0.0	0.0	3.2505861885427425	0.0017869656175051151
90-91	3.1910100312591345E-4	0.0	0.0	3.534330800522304	0.0017869656175051151
92-93	3.1910100312591345E-4	0.0	0.0	3.8094915955177795	0.0018507858181302979
94-95	3.1910100312591345E-4	0.0	0.0	4.0986928346507945	0.0018507858181302979
96-97	3.1910100312591345E-4	0.0	0.0	4.419389342792337	0.0018826959184428892
98-99	3.1910100312591345E-4	0.0	0.0	4.77751639860055	0.001978426219380663
100-101	3.1910100312591345E-4	0.0	0.0	5.1634371517810305	0.001978426219380663
102-103	3.829212037510961E-4	0.0	0.0	5.5590266653562255	0.0021060666206310285
104-105	3.829212037510961E-4	0.0	0.0	5.959849435382685	0.0021698868212562113
106-107	3.829212037510961E-4	0.0	0.0	6.399251516687068	0.0021698868212562113
108-109	3.829212037510961E-4	0.0	0.0	6.877903021375938	0.002265617122193985
110-111	3.829212037510961E-4	0.0	0.0	7.386199009255206	0.0023294373228191683
112-113	3.829212037510961E-4	0.0	0.0	7.905823082745443	0.0023613474231317594
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5935	0.0	40.199482	1
GGTATCA	4060	0.0	37.90311	1
TCAACGC	7260	0.0	32.19833	4
CAACGCA	7440	0.0	31.618319	5
TATCAAC	7635	0.0	31.009386	2
ATCAACG	7580	0.0	30.841007	3
AACGCAG	7700	0.0	30.643698	6
GTACATG	10135	0.0	29.249186	1
TACATGG	10415	0.0	28.17199	2
CATGGGG	7510	0.0	27.076864	4
ACATGGG	10545	0.0	27.03292	3
ACGCAGA	8870	0.0	26.60078	7
CGCAGAG	9130	0.0	25.972115	8
GAGTACT	3810	0.0	25.468122	12-13
GTACTTT	3945	0.0	24.436325	14-15
ATGGGGG	3990	0.0	24.329283	5
GCAGAGT	10010	0.0	23.509544	9
TATCACG	225	4.9776554E-6	21.204067	2
AGAGTAC	9180	0.0	21.0922	10-11
GTACTAG	345	1.2460077E-9	20.746428	1
>>END_MODULE