FastQCFastQC Report
Mon 15 Aug 2016
SRR2921610_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921610_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519178
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18690.12302705805376328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.046.197681
GTATCAA31150.037.8459631
GTACATG81550.033.1470071
TACATGG85250.031.4227872
ACATGGG86100.030.627593
TCAACGC36700.030.1688774
CATGGGG69800.029.9339454
CAACGCA37750.029.4854855
AACGCAG39150.028.7304296
ATGGGGG42950.028.4102025
TATCAAC41350.027.6409072
ATCAACG40550.027.5981043
GTATAGG4750.026.3231681
GTATATA3900.025.953551
GTATAGA4050.023.5221711
ACGCAGA47250.023.167817
CGCAGAG50550.021.5362638
GAGTACA60750.021.0719431
ATGGGGA25000.020.952045
AGTACAT60300.020.6326752
Produced by FastQC (version 0.11.5)