FastQCFastQC Report
Mon 15 Aug 2016
SRR2921610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519178
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36140.23789180727998957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28520.18773310303335092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17550.11552299993812443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.056.110421
GTATCAA45350.049.979671
TCAACGC58350.038.6247834
CAACGCA59700.037.7165155
TATCAAC61850.036.639122
AACGCAG61900.036.4890986
ATCAACG62950.035.998883
GTACATG80500.033.3429261
CATGGGG69450.032.022244
TACATGG83950.031.5401212
ACGCAGA73050.030.8369797
ACATGGG83650.030.7977433
ATGGGGG41150.028.9517145
CGCAGAG78300.028.843578
GCAGAGT83450.026.8493089
TAGGACG1159.929065E-425.923124
GAGTACT37600.025.73318712-13
AGAGTAC76000.024.5415710-11
GTACTTT41600.023.39310814-15
AGTACTT41550.023.35848212-13
Produced by FastQC (version 0.11.5)