##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921610_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1519178
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75245428777931	33.0	31.0	34.0	30.0	34.0
2	31.90600772259735	33.0	31.0	34.0	30.0	34.0
3	31.979538934871357	34.0	31.0	34.0	30.0	34.0
4	35.38336916411375	37.0	35.0	37.0	33.0	37.0
5	35.24992792154705	37.0	35.0	37.0	33.0	37.0
6	35.28593950149357	37.0	35.0	37.0	33.0	37.0
7	35.172045013816685	37.0	35.0	37.0	33.0	37.0
8	35.20818626915345	37.0	35.0	37.0	33.0	37.0
9	36.93437372052518	39.0	38.0	39.0	34.0	39.0
10-11	36.88716628334534	39.0	38.0	39.0	34.0	39.0
12-13	36.820943299600174	39.0	38.0	39.0	33.0	39.0
14-15	38.23114243360554	41.0	39.0	41.0	34.0	41.0
16-17	38.23153244715235	41.0	39.0	41.0	34.0	41.0
18-19	38.251472835967874	41.0	39.0	41.0	34.0	41.0
20-21	38.192664717366895	41.0	39.0	41.0	34.0	41.0
22-23	38.19564527659037	41.0	39.0	41.0	34.0	41.0
24-25	38.15206216783024	41.0	39.0	41.0	34.0	41.0
26-27	38.07174439071656	41.0	39.0	41.0	33.5	41.0
28-29	37.999607024325	41.0	38.5	41.0	33.0	41.0
30-31	37.876634930205675	41.0	38.0	41.0	33.0	41.0
32-33	37.74353531975845	41.0	38.0	41.0	33.0	41.0
34-35	37.604698396106315	41.0	38.0	41.0	33.0	41.0
36-37	37.51896749426334	40.0	38.0	41.0	32.5	41.0
38-39	37.427107620041895	40.0	38.0	41.0	32.0	41.0
40-41	37.298992613110514	40.0	38.0	41.0	32.0	41.0
42-43	37.12709307270116	40.0	37.0	41.0	31.5	41.0
44-45	36.94637297275237	40.0	37.0	41.0	31.0	41.0
46-47	36.714597631087344	40.0	37.0	41.0	30.5	41.0
48-49	36.49499466158673	40.0	36.0	41.0	30.0	41.0
50-51	36.229559340643426	40.0	36.0	41.0	29.5	41.0
52-53	36.090436077931614	40.0	35.0	41.0	29.0	41.0
54-55	35.94448971746563	39.5	35.0	41.0	29.0	41.0
56-57	35.73584925532097	39.0	35.0	41.0	29.0	41.0
58-59	35.52738191311354	39.0	35.0	41.0	28.5	41.0
60-61	35.260613634478645	38.5	35.0	41.0	28.0	41.0
62-63	34.89865440389474	37.0	35.0	40.5	28.0	41.0
64-65	34.59934253918895	37.0	35.0	40.0	28.0	41.0
66-67	34.28811962785137	36.0	35.0	39.0	28.0	41.0
68-69	33.91614215055773	36.0	35.0	39.0	27.0	41.0
70-71	33.54614765353369	35.0	34.0	38.5	26.0	40.5
72-73	33.23495600910493	35.0	34.0	37.0	26.0	39.5
74-75	32.92184227259742	35.0	34.0	37.0	26.0	39.0
76-77	32.609711633528136	35.0	34.0	36.0	26.0	39.0
78-79	32.360705262977746	35.0	34.0	36.0	26.0	37.5
80-81	32.12324526816475	35.0	34.0	35.5	25.5	37.0
82-83	31.91542037865214	35.0	34.0	35.0	25.0	37.0
84-85	31.722997239296514	35.0	34.0	35.0	24.5	36.0
86-87	31.603582990275005	35.0	33.0	35.0	24.0	36.0
88-89	31.507460613568654	35.0	33.0	35.0	24.0	36.0
90-91	31.411385301788204	35.0	33.0	35.0	24.0	35.0
92-93	31.335888882013826	35.0	33.0	35.0	24.0	35.0
94-95	31.27414990211812	35.0	33.0	35.0	23.0	35.0
96-97	31.20448788752865	35.0	33.0	35.0	23.0	35.0
98-99	31.127100642584345	35.0	33.0	35.0	21.5	35.0
100-101	31.057339890388093	35.0	33.0	35.0	20.0	35.0
102-103	30.354662521442517	34.0	31.5	35.0	19.5	35.0
104-105	30.877648307176646	35.0	33.0	35.0	20.0	35.0
106-107	30.97050576035198	35.0	33.0	35.0	20.0	35.0
108-109	30.945657125103182	35.0	33.0	35.0	19.5	35.0
110-111	30.862748144062117	35.0	33.0	35.0	19.0	35.0
112-113	30.813644944832006	35.0	33.0	35.0	18.5	35.0
114-115	30.673275942647933	35.0	33.0	35.0	17.0	35.0
116-117	30.847875298352136	35.0	33.0	35.0	18.0	35.0
118-119	30.812749394738468	35.0	33.0	35.0	13.0	35.0
120-121	30.702705015475473	35.0	33.0	35.0	2.0	35.0
122-123	30.54120353243662	35.0	33.0	35.0	2.0	35.0
124-125	29.885882694457134	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23872.0
3	6321.0
4	3416.0
5	2827.0
6	3133.0
7	3496.0
8	3763.0
9	4046.0
10	4367.0
11	4853.0
12	5444.0
13	6901.0
14	9618.0
15	8760.0
16	4724.0
17	4057.0
18	3882.0
19	3542.0
20	3500.0
21	3552.0
22	3865.0
23	4541.0
24	5817.0
25	7556.0
26	9194.0
27	11089.0
28	13158.0
29	16005.0
30	19579.0
31	24693.0
32	32347.0
33	43773.0
34	67296.0
35	120831.0
36	283623.0
37	560999.0
38	180545.0
39	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.35757647585562	17.466914233792913	10.99955227221849	27.175957018132973
2	16.677593858654795	19.64353656593902	36.37155349123784	27.30731608416834
3	19.29626673830821	22.831526491566315	28.60051413340076	29.271692636724715
4	14.050663042697034	14.901465083355916	33.058766201867506	37.989105672079546
5	15.06081052609401	36.80189041990603	32.26717390388302	15.870125150116934
6	35.34738644972168	32.975198445374	16.04433319060637	15.633081914297946
7	30.54087394185356	31.526358738068378	18.89037955291934	19.042387767158715
8	28.542583443423375	33.43690358746462	18.501581213523753	19.51893175558825
9	28.154837944976357	14.753065282954262	18.45148885172064	38.640607920348735
10-11	27.152944942613495	24.55511419830645	25.81669845382979	22.475242405250267
12-13	27.025868172761854	22.129258533533122	26.39963116036291	24.445242133342116
14-15	24.637660158507728	23.577275399356814	23.882138487606394	27.902925954529064
16-17	24.549397803019975	26.453758898598945	24.781655434130446	24.215187864250634
18-19	24.54751120047861	25.489941711744606	26.71447908184885	23.248068005927934
20-21	25.168465641700273	24.398934277247914	26.774358260223742	23.658241820828078
22-23	25.28916278889125	23.95431718348762	26.428435542255517	24.328084485365608
24-25	24.700076657407873	24.39417114054308	26.566251716069388	24.33950048597966
26-27	24.187975871565158	24.633101850709014	26.871765603847752	24.307156673878076
28-29	24.287029911730805	24.895941881491908	26.729063137917574	24.087965068859717
30-31	24.868634599630514	24.53647309119861	26.444130743667227	24.15076156550365
32-33	24.497943585616454	24.608145672148794	26.377238068435233	24.51667267379952
34-35	24.57625696089916	24.71113852481436	26.5834183885899	24.129186125696584
36-37	24.602347290662284	24.592538660977223	26.594782796453487	24.210331251907004
38-39	24.55241123267112	24.827369432705346	26.562989586213252	24.057229748410286
40-41	24.64240928165885	24.580037850736446	26.50078169999177	24.276771167612935
42-43	24.549548734115344	24.51159901929774	26.239841539369458	24.699010707217457
44-45	24.49813869442003	24.572426345949395	26.277882555073912	24.65155240455666
46-47	24.518341293641395	24.605169049163667	26.175836408345866	24.700653248849072
48-49	24.432305334205314	24.983568097523055	25.971160818050375	24.612965750221257
50-51	24.42560135443806	25.026231705278068	26.044956600844944	24.503210339438926
52-53	24.733455198685064	24.871820864515527	25.685064736305453	24.709659200493956
54-55	24.35100275262846	24.684721513191914	25.915488414636755	25.04878731954287
56-57	24.37487925122098	24.92880141840937	25.695406000593092	25.00091332977656
58-59	24.361154619984053	24.88157157230626	25.9487686175281	24.80850519018159
60-61	24.618436369967544	24.81551682770238	26.053588901618742	24.51245790071134
62-63	24.480428499819475	24.800868637645596	26.020489605936962	24.69821325659797
64-65	24.27378490209837	24.62331603011629	26.04862629658934	25.054272771196
66-67	24.39066937173085	24.869596070372044	25.76659210625057	24.973142451646538
68-69	24.177084622412735	24.938848267345634	25.887753328787	24.996313781454635
70-71	24.115508518422462	24.776754271059744	26.00353612282432	25.10420108769348
72-73	24.301867665475754	24.967934212886547	25.771380894155747	24.95881722748195
74-75	24.11216491316419	25.057789056367163	25.74938162500555	25.080664405463093
76-77	24.138128513271067	24.812123617771906	25.662039409525256	25.387708459431767
78-79	23.961188157655254	24.90156667218501	25.7679576849416	25.369287485218134
80-81	24.13051663465374	25.093405776018347	25.564515810523847	25.211561778804064
82-83	24.107934568592846	24.953837403651008	25.722094916222705	25.21613311153344
84-85	24.10992078786241	24.898619193581144	25.50043659326261	25.49102342529384
86-87	23.828807420855224	25.11535843726015	25.633697960344342	25.422136181540278
88-89	23.755306799582268	24.902454284754167	25.710927654403637	25.631311261259924
90-91	23.821524441700248	24.76737236088749	25.78230423798321	25.628798959429055
92-93	23.905855666682903	24.816512614058393	25.788617265389576	25.48901445386913
94-95	23.873075493862085	24.64997085490416	25.752975317674963	25.723978333558794
96-97	23.680821351073014	24.553411418383437	25.823228760979372	25.942538469564173
98-99	23.48502027915892	24.686235021019694	25.753198867408233	26.075545832413148
100-101	23.457619844415863	24.809732631725844	25.864776872756188	25.86787065110211
102-103	23.334023406991292	24.71458761955197	25.906626780628656	26.04476219282808
104-105	23.37365996611325	24.789491422334972	25.822418439445542	26.01443017210623
106-107	23.42004082495101	24.74053248848548	25.688814081909257	26.150612604654256
108-109	23.300357341395813	24.797425904404182	25.706791790462404	26.1954249637376
110-111	23.07492806960611	25.10016936801966	25.747230243743818	26.07767231863042
112-113	22.967192358213197	25.20982626408373	25.68110225724711	26.141879120455958
114-115	22.92506210595467	25.249279544595826	25.640280467463327	26.185377881986177
116-117	22.780413935015698	25.229478429241585	25.628615710809623	26.361491924933095
118-119	22.885985275236045	25.147394103840888	25.54248567365186	26.424134947271206
120-121	22.687079240801804	25.33270709729062	25.596312097261	26.383901564646585
122-123	22.521309487683645	25.42646951372437	25.482882305275485	26.569338693316503
124-125	22.298397539603513	25.39458536164257	25.522560773493407	26.784456325260514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	32.0
2	43.0
3	108.5
4	119.0
5	120.5
6	302.0
7	720.5
8	1203.0
9	1299.0
10	966.5
11	701.5
12	640.0
13	641.5
14	679.0
15	729.5
16	779.5
17	828.0
18	906.0
19	966.0
20	982.5
21	1036.0
22	1111.5
23	1228.5
24	1364.5
25	1548.0
26	2011.0
27	2721.5
28	3490.0
29	4525.5
30	5994.0
31	7703.0
32	9955.0
33	11952.0
34	13790.0
35	16106.5
36	19048.5
37	22392.5
38	25143.0
39	27824.0
40	30497.5
41	33264.5
42	36307.0
43	40512.5
44	45027.0
45	49153.0
46	53725.5
47	57719.0
48	61815.5
49	65800.5
50	68411.0
51	70051.5
52	70337.5
53	70334.5
54	70854.0
55	69360.0
56	65732.0
57	60470.5
58	55354.5
59	49940.5
60	42988.0
61	36609.5
62	30464.0
63	23695.0
64	17245.5
65	12733.0
66	9619.0
67	7543.5
68	5906.0
69	4293.5
70	3251.5
71	2435.5
72	1811.5
73	1415.5
74	1038.0
75	689.0
76	439.0
77	265.0
78	144.0
79	75.5
80	43.5
81	23.0
82	13.0
83	7.0
84	7.0
85	5.5
86	4.5
87	3.5
88	2.0
89	1.0
90	1.5
91	1.0
92	0.5
93	0.5
94	0.5
95	0.5
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026198378333546168
2	0.0021064022780740636
3	0.03330748602204613
4	0.17173761073422603
5	0.023828675770712845
6	0.07425068030211075
7	0.05522723472825436
8	0.04765735154142569
9	0.03673038972391649
10-11	0.0663187592237381
12-13	0.05944003928440248
14-15	0.06289585552186774
16-17	0.05404238344683769
18-19	0.03979125553424286
20-21	0.02609964072676145
22-23	0.0033570786306805394
24-25	0.005364743301969881
26-27	0.0037849415934143333
28-29	0.0049368803392360865
30-31	0.0024684401696180433
32-33	0.009906673214067081
34-35	0.004443192305312478
36-37	0.007076195152905058
38-39	0.002501352705212951
40-41	0.003488728773060168
42-43	0.004114066949363406
44-45	0.005463480908754603
46-47	0.005167268088400437
48-49	0.0019418396000995277
50-51	0.0015798017085555478
52-53	9.873760678472173E-4
54-55	0.005562218515539325
56-57	7.569883186828667E-4
58-59	7.569883186828667E-4
60-61	9.873760678472174E-5
62-63	0.0011519387458217537
64-65	0.0
66-67	0.003422903701870353
68-69	3.9495042713888696E-4
70-71	0.0
72-73	0.0023367900272384147
74-75	0.004969792874830995
76-77	0.0018101894577198985
78-79	1.6456267797453623E-4
80-81	0.0
82-83	0.0059900814782731186
84-85	0.0019418396000995277
86-87	0.0
88-89	0.001217763817011568
90-91	7.899008542777739E-4
92-93	0.0
94-95	0.0035874663798448896
96-97	0.0014810641017708262
98-99	4.936880339236087E-4
100-101	0.0
102-103	0.0010861136746319392
104-105	0.0
106-107	5.924256407083304E-4
108-109	0.002797565525567116
110-111	6.582507118981449E-5
112-113	2.962128203541652E-4
114-115	0.0
116-117	0.001744364386530084
118-119	0.002402615098428229
120-121	0.0032254284883009104
122-123	0.0012506763526064755
124-125	0.11183679595149483
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1519178.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.60242065803042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24365897629464	45.85267817152297
2	11.650203893536776	13.654602986417322
3	3.8595073105939814	6.785294128445166
4	1.8569258509169455	4.352813993848238
5	1.0420018635246082	3.053191576636035
6	0.7115267105362503	2.501831256016199
7	0.5080104572120309	2.083944975855246
8	0.3664628519149937	1.718048816277118
9	0.2614108694758813	1.3787378763846372
>10	1.4325799203374578	14.599363819866367
>50	0.053074394101257534	2.084266084845941
>100	0.013848818972870871	1.218061805461871
>500	4.5033290416499527E-4	0.1747717371492307
>1k	3.3774967812374643E-4	0.5423927712736223
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3614	0.23789180727998957	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2852	0.18773310303335092	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1755	0.11552299993812443	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.582507118981449E-5
2	0.0	0.0	0.0	0.0	6.582507118981449E-5
3	0.0	0.0	0.0	0.0	6.582507118981449E-5
4	0.0	0.0	0.0	0.0	6.582507118981449E-5
5	0.0	0.0	0.0	0.0	6.582507118981449E-5
6	0.0	0.0	0.0	0.0	1.9747521356944348E-4
7	0.0	0.0	0.0	0.0	1.9747521356944348E-4
8	0.0	0.0	0.0	0.0	1.9747521356944348E-4
9	0.0	0.0	0.0	0.0	1.9747521356944348E-4
10-11	0.0	0.0	0.0	0.0	2.6330028475925795E-4
12-13	0.0	0.0	0.0	0.0	4.6077549832870146E-4
14-15	0.0	0.0	0.0	0.0	6.911632474930522E-4
16-17	0.0	0.0	0.0	0.0	7.899008542777739E-4
18-19	0.0	0.0	0.0	0.0	7.899008542777739E-4
20-21	0.0	0.0	0.0	0.0	8.228133898726812E-4
22-23	0.0	0.0	0.0	9.873760678472174E-5	9.215509966574028E-4
24-25	0.0	0.0	0.0	7.240757830879594E-4	0.001086113674631939
26-27	0.0	0.0	0.0	0.002007664671289342	0.0011848512814166608
28-29	0.0	0.0	0.0	0.007866096007182832	0.0012506763526064753
30-31	0.0	0.0	0.0	0.026593328760685053	0.0013165014237962898
32-33	0.0	0.0	0.0	0.06230342988115942	0.0013165014237962898
34-35	0.0	0.0	0.0	0.12138143127401793	0.0013823264949861043
36-37	0.0	0.0	0.0	0.19408522240316803	0.0013823264949861043
38-39	0.0	0.0	0.0	0.29186836565563745	0.001481064101770826
40-41	0.0	0.0	0.0	0.40235574764774107	0.0015139766373657333
42-43	3.2912535594907244E-5	0.0	0.0	0.5207750507182174	0.0015139766373657333
44-45	6.582507118981449E-5	0.0	0.0	0.6647015688747467	0.0015798017085555478
46-47	6.582507118981449E-5	0.0	0.0	0.8261375559677668	0.0016456267797453623
48-49	6.582507118981449E-5	0.0	0.0	0.9739477533244953	0.0018431019933148058
50-51	6.582507118981449E-5	0.0	0.0	1.1107322512569298	0.0018431019933148058
52-53	6.582507118981449E-5	0.0	0.0	1.2607146759629222	0.0019418396000995277
54-55	6.582507118981449E-5	0.0	0.0	1.4427539103383542	0.002139314813668971
56-57	6.582507118981449E-5	0.0	0.0	1.653558700823735	0.0021722273492638783
58-59	6.582507118981449E-5	0.0	0.0	1.858603797580007	0.0021722273492638783
60-61	6.582507118981449E-5	0.0	0.0	2.0550258100104135	0.0021722273492638783
62-63	6.582507118981449E-5	0.0	0.0	2.2524022859730723	0.0023038774916435073
64-65	6.582507118981449E-5	0.0	0.0	2.4822305220323093	0.0023038774916435073
66-67	6.582507118981449E-5	0.0	0.0	2.7714658848403544	0.0023367900272384147
68-69	6.582507118981449E-5	0.0	0.0	3.0729776234252997	0.0023697025628333216
70-71	6.582507118981449E-5	0.0	0.0	3.3626079366604835	0.0024355276340231363
72-73	6.582507118981449E-5	0.0	0.0	3.6472684570208362	0.002665915383187487
74-75	6.582507118981449E-5	0.0	0.0	3.956745029219749	0.0027646529899722086
76-77	6.582507118981449E-5	0.0	0.0	4.335667051523917	0.0028304780611620233
78-79	6.582507118981449E-5	0.0	0.0	4.749805486914634	0.0028304780611620233
80-81	6.582507118981449E-5	0.0	0.0	5.1534448234505765	0.002929215667946745
82-83	6.582507118981449E-5	0.0	0.0	5.563699579641096	0.0030279532747314666
84-85	6.582507118981449E-5	0.0	0.0	5.996696897927695	0.0030279532747314666
86-87	6.582507118981449E-5	0.0	0.0	6.489331730712267	0.0030937783459212814
88-89	6.582507118981449E-5	0.0	0.0	7.041439515316836	0.0031596034171110956
90-91	6.582507118981449E-5	0.0	0.0	7.615006273129284	0.0031596034171110956
92-93	6.582507118981449E-5	0.0	0.0	8.162703777964136	0.00322542848830091
94-95	6.582507118981449E-5	0.0	0.0	8.725573961708239	0.0034229037018703536
96-97	6.582507118981449E-5	0.0	0.0	9.362563175612074	0.003620378915439797
98-99	6.582507118981449E-5	0.0	0.0	10.083676830496493	0.003752029057819426
100-101	6.582507118981449E-5	0.0	0.0	10.828487511009243	0.003752029057819426
102-103	6.582507118981449E-5	0.0	0.0	11.55295824452434	0.003982416806983777
104-105	6.582507118981449E-5	0.0	0.0	12.297801837572687	0.004048241878173591
106-107	6.582507118981449E-5	0.0	0.0	13.11103767958725	0.004146979484958313
108-109	6.582507118981449E-5	0.0	0.0	14.004744671131363	0.004212804556148127
110-111	6.582507118981449E-5	0.0	0.0	14.947293865498317	0.004245717091743035
112-113	6.582507118981449E-5	0.0	0.0	15.85591023566692	0.004311542162932849
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2530	0.0	56.11042	1
GTATCAA	4535	0.0	49.97967	1
TCAACGC	5835	0.0	38.624783	4
CAACGCA	5970	0.0	37.716515	5
TATCAAC	6185	0.0	36.63912	2
AACGCAG	6190	0.0	36.489098	6
ATCAACG	6295	0.0	35.99888	3
GTACATG	8050	0.0	33.342926	1
CATGGGG	6945	0.0	32.02224	4
TACATGG	8395	0.0	31.540121	2
ACGCAGA	7305	0.0	30.836979	7
ACATGGG	8365	0.0	30.797743	3
ATGGGGG	4115	0.0	28.951714	5
CGCAGAG	7830	0.0	28.84357	8
GCAGAGT	8345	0.0	26.849308	9
TAGGACG	115	9.929065E-4	25.92312	4
GAGTACT	3760	0.0	25.733187	12-13
AGAGTAC	7600	0.0	24.54157	10-11
GTACTTT	4160	0.0	23.393108	14-15
AGTACTT	4155	0.0	23.358482	12-13
>>END_MODULE