FastQCFastQC Report
Mon 15 Aug 2016
SRR2921609_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921609_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117163
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19450.045.929681
GGTATCA12900.041.088671
GTACATG55700.038.705721
TACATGG58500.035.924012
TCAACGC24400.035.378894
ATGGGGG31500.035.1487585
ACATGGG60400.034.892523
CAACGCA24600.034.844565
CATGGGG51700.033.970154
TATCAAC26900.033.1974682
ATCAACG26650.033.0621073
AACGCAG26050.032.896196
GTATAGG2751.8189894E-1228.1535021
ACGCAGA32600.026.4632997
TGGGGGG28050.025.6710346
CGCAGAG34450.025.2126398
GAGTACA39050.023.0291371
AGTACAT39150.022.2017422
CCCTAGA4600.022.0096361
TACGTCT2255.067768E-621.1591576
Produced by FastQC (version 0.11.5)