##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921609_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1117163
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.983123322200967	31.0	30.0	33.0	23.0	34.0
2	29.387218337879073	31.0	30.0	34.0	24.0	34.0
3	29.461094755196868	31.0	30.0	34.0	23.0	34.0
4	32.89337276655242	37.0	35.0	37.0	25.0	37.0
5	32.8939250583845	37.0	35.0	37.0	26.0	37.0
6	32.865706257726046	37.0	35.0	37.0	25.0	37.0
7	32.809653559954995	37.0	35.0	37.0	25.0	37.0
8	32.70608228163661	37.0	35.0	37.0	24.0	37.0
9	34.422311694891434	39.0	35.0	39.0	23.0	39.0
10-11	34.453855435598925	39.0	35.0	39.0	25.0	39.0
12-13	34.37718891513593	39.0	35.0	39.0	23.0	39.0
14-15	35.59012740307368	40.0	37.0	41.0	23.5	41.0
16-17	35.55255276087733	40.0	36.5	41.0	22.5	41.0
18-19	35.4950508564999	40.0	36.5	41.0	22.0	41.0
20-21	35.55040983276388	40.0	37.0	41.0	22.0	41.0
22-23	35.4835001696261	40.0	36.5	41.0	22.0	41.0
24-25	35.49040336996481	40.0	36.5	41.0	23.0	41.0
26-27	35.45994407261966	40.0	36.0	41.0	23.5	41.0
28-29	35.407762788420314	40.0	36.0	41.0	23.0	41.0
30-31	35.30546213936552	40.0	36.0	41.0	23.0	41.0
32-33	35.21411244375261	40.0	36.0	41.0	22.5	41.0
34-35	35.0742044804563	40.0	35.5	41.0	20.5	41.0
36-37	34.964339581600896	40.0	35.0	41.0	19.0	41.0
38-39	34.83813015647672	40.0	35.0	41.0	17.5	41.0
40-41	34.72146723441432	40.0	35.0	41.0	16.0	41.0
42-43	34.45959676430387	40.0	35.0	41.0	14.5	41.0
44-45	34.08129207644721	39.0	34.0	41.0	9.0	41.0
46-47	33.81396358454406	39.0	34.0	40.5	8.5	41.0
48-49	33.61628159901464	38.5	33.5	40.0	7.5	41.0
50-51	33.41344101084623	38.0	33.0	40.0	4.0	41.0
52-53	33.20513210695306	38.0	33.0	40.0	2.0	41.0
54-55	32.973855202866545	38.0	33.0	40.0	2.0	41.0
56-57	32.72359852590893	37.5	32.5	40.0	2.0	41.0
58-59	32.731831881292166	37.5	33.0	40.0	2.0	41.0
60-61	32.516831921572766	37.0	32.5	40.0	2.0	41.0
62-63	32.2194581274174	36.5	32.0	40.0	2.0	41.0
64-65	31.92422457600189	36.0	32.0	39.0	2.0	41.0
66-67	31.609607998116658	35.5	31.5	39.0	2.0	41.0
68-69	31.281549782798034	35.0	31.0	38.5	2.0	40.0
70-71	30.923198315733693	35.0	31.0	37.5	2.0	40.0
72-73	30.577059032567316	35.0	31.0	37.0	2.0	39.0
74-75	30.21282480712304	35.0	30.5	36.5	2.0	39.0
76-77	29.917919766408303	35.0	30.0	36.0	2.0	38.5
78-79	29.652772245410922	35.0	30.0	36.0	2.0	37.0
80-81	29.39548749824332	35.0	30.0	35.0	2.0	37.0
82-83	29.172992213311755	34.0	30.0	35.0	2.0	36.5
84-85	28.961312270456503	34.0	30.0	35.0	2.0	36.0
86-87	28.79233155770465	34.0	29.0	35.0	2.0	36.0
88-89	28.636126509739405	34.0	29.0	35.0	2.0	35.5
90-91	28.454221989092012	34.0	29.0	35.0	2.0	35.0
92-93	28.37769018487007	34.0	29.0	35.0	2.0	35.0
94-95	28.256409762944173	34.0	29.0	35.0	2.0	35.0
96-97	28.173934331874577	34.0	29.0	35.0	2.0	35.0
98-99	28.089088163499866	34.0	29.0	35.0	2.0	35.0
100-101	27.985977874311985	34.0	29.0	35.0	2.0	35.0
102-103	27.128028765721744	32.5	26.0	34.0	2.0	35.0
104-105	28.04117349035011	34.0	29.0	35.0	2.0	35.0
106-107	28.326808173919115	34.0	29.0	35.0	2.0	35.0
108-109	28.339475528638168	34.0	29.0	35.0	2.0	35.0
110-111	28.290466565756297	34.0	29.0	35.0	2.0	35.0
112-113	28.220191681965836	34.0	29.0	35.0	2.0	35.0
114-115	28.109781652274556	34.0	29.0	35.0	2.0	35.0
116-117	28.003312855867946	34.0	29.0	35.0	2.0	35.0
118-119	27.733825323609892	34.0	29.0	35.0	2.0	35.0
120-121	27.617545514844295	34.0	29.0	35.0	2.0	35.0
122-123	27.371323164121975	34.0	28.5	35.0	2.0	35.0
124-125	26.59322677174235	33.5	25.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62728.0
3	9827.0
4	4985.0
5	4207.0
6	4423.0
7	4840.0
8	4981.0
9	5117.0
10	5368.0
11	5821.0
12	5730.0
13	5895.0
14	5946.0
15	5296.0
16	4551.0
17	4106.0
18	3787.0
19	3430.0
20	3354.0
21	3539.0
22	3922.0
23	5339.0
24	7809.0
25	9769.0
26	10614.0
27	11677.0
28	13792.0
29	16803.0
30	20294.0
31	25179.0
32	32866.0
33	45388.0
34	70074.0
35	112464.0
36	211127.0
37	292127.0
38	69927.0
39	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80257339346454	18.201865714738503	12.944145847714772	25.051415044082187
2	16.139572816298834	19.313863372475577	38.847415081147716	25.699148730077876
3	19.70174961147596	23.578395914947254	28.049287049437442	28.670567424139335
4	13.492064202566276	15.274205547403957	35.1151762670016	36.118553983028164
5	14.58917692138966	37.59070337924644	32.42339733116078	15.396722368203122
6	34.14518978858222	33.97683328494988	17.193329534336126	14.684647392131774
7	29.310741689124857	32.97597264354659	19.329182933743176	18.384102733585365
8	27.011616233531875	35.183637866539144	18.6174971588543	19.187248741074683
9	27.456879261933416	16.414263509254486	18.12082760300269	38.00802962580941
10-11	26.289570147761985	25.95547348048931	26.190518837212846	21.56443753453586
12-13	25.864333309161815	23.402240073338184	26.586479883475363	24.146946734024638
14-15	23.71919881093084	24.664007377959244	23.704962214820384	27.91183159628953
16-17	24.322343469545917	27.568217849398913	24.399619796887727	23.70981888416744
18-19	24.414890925755365	26.91741288007855	25.55714705960051	23.110549134565574
20-21	25.01536727220957	25.79951227658135	25.536802434408724	23.648318016800356
22-23	25.058608406299083	25.221941841118834	25.482523143380607	24.236926609201475
24-25	24.68711860794755	25.531721521458238	25.37882891877396	24.40233095182025
26-27	24.280474065901394	25.774691529394484	25.56853698333748	24.376297421366637
28-29	24.428155644114092	26.177002641830477	25.38969238346841	24.005149330587024
30-31	24.98077207182605	25.670067555165577	25.435661427298733	23.913498945709645
32-33	24.511440645147715	25.767317213594374	25.43333606427839	24.28790607697952
34-35	24.508803037630074	26.062319775043434	25.5286747980585	23.900202389267996
36-37	24.651370147213445	25.987790177981946	25.402096501369904	23.95874317343471
38-39	24.642016979767977	26.10855322796894	25.320715272166478	23.928714520096605
40-41	24.58078171097389	25.87876777068689	25.462672546535902	24.077777971803314
42-43	24.488332334298644	25.825994270808383	25.336698565642845	24.348974829250125
44-45	24.347718654751837	25.82244948112483	25.624953278181817	24.20487858594151
46-47	24.25593446072232	25.8139696254099	25.553827041667134	24.376268872200647
48-49	24.23601401653887	26.045028601206905	25.468661789441217	24.25029559281301
50-51	24.294827398638922	26.024803093380278	25.496838615141964	24.183530892838835
52-53	24.40799402252477	25.88172460367665	25.43932338848351	24.27095798531507
54-55	24.151429076656886	25.62583238762907	25.68205982222998	24.540678713484066
56-57	24.251300509459472	25.85614261283766	25.43326439066319	24.459292487039676
58-59	24.284396934536602	26.021477940123187	25.54670713364847	24.147417991691736
60-61	24.628064691875554	25.806510820851926	25.58433227131121	23.98109221596131
62-63	24.429656390386718	25.913147415709208	25.54023775788628	24.11695843601779
64-65	24.26363644095908	25.669013438240455	25.574826230421127	24.492523890379342
66-67	24.290612731700044	25.892741621012753	25.445313126076428	24.37133252121077
68-69	24.106222342594812	25.890532074196273	25.575419519608918	24.4278260636
70-71	24.059334962322353	25.744429623241437	25.794400809210817	24.401834605225392
72-73	24.169179556418253	26.063471655677994	25.445784254388006	24.32156453351574
74-75	24.126342702297197	26.02593242779912	25.511142224613426	24.336582645290257
76-77	24.063192301683614	25.67863190779711	25.605219112573597	24.652956677945678
78-79	24.01683639002513	25.657856153801728	25.767179873333117	24.558127582840026
80-81	24.163410015712504	25.950292986673475	25.499062174055126	24.387234823558902
82-83	24.243718433241202	25.921653106558544	25.566466913604785	24.26816154659547
84-85	24.247475545501057	26.042334830019243	25.37883043642323	24.331359188056474
86-87	23.940166593850496	26.146496587189606	25.494194999319586	24.41914181964031
88-89	23.953906574907187	25.75759542772419	25.63847165225706	24.650026345111563
90-91	24.28142376289659	25.667565124783607	25.568721302052488	24.482289810267314
92-93	24.21006693835897	25.866450028982	25.57739090345524	24.34609212920379
94-95	24.223313899488602	25.746987008045835	25.561943981289797	24.467755111175766
96-97	23.92305688279389	25.76977585115593	25.68539661820568	24.621770647844503
98-99	23.912035420728575	25.77330662097636	25.534829010479047	24.779828947816014
100-101	23.81873604017743	25.846362501063076	25.677434660197218	24.65746679856228
102-103	23.572403341289853	25.8702450556725	25.817072302859113	24.740279300178543
104-105	23.761723916453878	25.905966972739865	25.668254845661675	24.664054265144582
106-107	23.696146444076444	25.81524414805532	25.796759611511433	24.691849796356802
108-109	23.647852288938637	25.842989852653307	25.82284645854512	24.686311399862934
110-111	23.514036443775677	26.13079495911678	25.770949263099567	24.58421933400797
112-113	23.520568239290686	26.18161636686703	25.79066093175847	24.507154462083815
114-115	23.53840376040784	26.292326857542907	25.65969848877334	24.50957089327591
116-117	23.37550789855546	26.173011799270075	25.733482051341728	24.717998250832736
118-119	23.338192315149254	26.013384939071237	25.845130725759546	24.803292020019963
120-121	23.243153004004384	26.125978549890995	26.01274403045964	24.61812441564498
122-123	23.303133093945377	26.143071526812093	25.79132966836727	24.762465710875265
124-125	23.18749163837557	26.07744862034319	25.893470435540745	24.8415893057405
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	526.0
1	408.5
2	259.5
3	210.5
4	165.5
5	141.5
6	154.5
7	180.0
8	196.5
9	216.5
10	252.5
11	268.0
12	286.0
13	321.5
14	354.5
15	388.5
16	419.0
17	465.0
18	530.0
19	577.0
20	622.5
21	736.0
22	909.0
23	1108.5
24	1376.0
25	1757.5
26	2297.5
27	2954.0
28	3697.0
29	4730.0
30	6035.0
31	7316.0
32	8915.5
33	10644.5
34	12355.5
35	14029.5
36	16081.5
37	18501.5
38	20653.5
39	22833.0
40	24936.5
41	27159.0
42	29622.0
43	32644.5
44	35830.5
45	38652.5
46	40915.0
47	43498.0
48	46784.5
49	49319.0
50	50410.5
51	50480.5
52	50325.0
53	49655.0
54	49053.0
55	47529.0
56	44273.0
57	40238.5
58	36400.5
59	32513.5
60	27866.5
61	23458.0
62	19369.0
63	15037.5
64	11161.0
65	8465.0
66	6505.0
67	5044.5
68	3827.5
69	2958.0
70	2363.5
71	1784.0
72	1357.5
73	1052.0
74	797.5
75	599.5
76	443.0
77	302.0
78	200.5
79	144.5
80	103.0
81	81.0
82	60.5
83	45.5
84	39.5
85	37.0
86	29.0
87	24.5
88	23.5
89	20.0
90	20.0
91	19.5
92	16.0
93	15.0
94	12.5
95	9.0
96	7.0
97	4.5
98	2.5
99	2.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024347387086754574
2	0.011278569018128957
3	0.010293932040355794
4	0.012173693543377287
5	0.017723465599916933
6	0.0965839362742948
7	0.05764601942599244
8	0.04797867455331048
9	0.026764223304925065
10-11	0.051022097939154805
12-13	0.013068818068625617
14-15	0.028912522165521058
16-17	0.03504412516347211
18-19	0.042026096460409085
20-21	0.030568502537230466
22-23	0.038400842133153354
24-25	0.032761557624088875
26-27	0.04699403757553732
28-29	0.045919888145239326
30-31	0.02667471085240023
32-33	0.0298524029170318
34-35	0.04502476361999099
36-37	0.009980638456518878
38-39	0.0219753070948465
40-41	0.04220512136545876
42-43	0.022512381809995496
44-45	0.01606748522820752
46-47	0.024660680670591486
48-49	0.030389477632180802
50-51	0.02922581574935797
52-53	0.027167029341286814
54-55	0.024213118407967325
56-57	0.026227148589776067
58-59	0.018171027862541097
60-61	0.02403409350291766
62-63	0.024213118407967325
64-65	0.02134871992717267
66-67	0.030120940274606305
68-69	0.023317993882718994
70-71	0.04650171908665074
72-73	0.022825675393832415
74-75	0.009488319967632297
76-77	0.017141634658505517
78-79	0.02654044217361298
80-81	0.019513714650413592
82-83	0.02542153651705257
84-85	0.012486987127214202
86-87	0.017454928242342432
88-89	0.023049456525144497
90-91	0.022333356904945832
92-93	0.008100876953497387
94-95	0.01123381279186654
96-97	0.016291266359519604
98-99	0.00689245884441214
100-101	0.010562469397930293
102-103	0.004430866399979233
104-105	0.004967941115128231
106-107	0.001163661882822829
108-109	0.015351385608008857
110-111	0.0015664679191845774
112-113	0.0025063486706953238
114-115	0.014008698820136363
116-117	0.00537074715148998
118-119	0.27525079151386145
120-121	0.0011189056565604123
122-123	7.608558464610804E-4
124-125	0.3082808864955248
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1117163.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.66993286514763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.18135829415323	60.19760872009895
2	8.486856221391555	11.995311188038036
3	2.5270514382161204	5.35759666456524
4	1.233118246874433	3.4857753488561896
5	0.6891244380098053	2.4350188884942767
6	0.43503352602311285	1.8446274046885105
7	0.2827288827847405	1.3986301815805064
8	0.2140906963197203	1.2103820108773877
9	0.15650160915226308	0.9953962390870206
>10	0.7627297685215739	9.149530294712454
>50	0.02533684136139034	1.1783559331059772
>100	0.005817120087120987	0.6435422670227375
>500	2.5291710493527804E-4	0.1082248588727839
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.951245252483299E-5	0.0
8	0.0	0.0	0.0	8.951245252483299E-5	0.0
9	0.0	0.0	0.0	8.951245252483299E-5	8.951245252483299E-5
10-11	0.0	0.0	0.0	8.951245252483299E-5	1.7902490504966597E-4
12-13	0.0	0.0	0.0	8.951245252483299E-5	2.2378113131208248E-4
14-15	0.0	0.0	0.0	8.951245252483299E-5	2.68537357574499E-4
16-17	0.0	0.0	0.0	8.951245252483299E-5	2.68537357574499E-4
18-19	0.0	0.0	0.0	8.951245252483299E-5	2.68537357574499E-4
20-21	0.0	0.0	0.0	1.7902490504966597E-4	2.68537357574499E-4
22-23	0.0	0.0	0.0	1.7902490504966597E-4	2.68537357574499E-4
24-25	0.0	0.0	0.0	2.68537357574499E-4	2.68537357574499E-4
26-27	0.0	0.0	0.0	6.265871676738309E-4	2.68537357574499E-4
28-29	0.0	0.0	0.0	0.00255110489695774	2.68537357574499E-4
30-31	0.0	0.0	0.0	0.009533076193894714	2.68537357574499E-4
32-33	0.0	0.0	0.0	0.022243844452421	3.5804981009933195E-4
34-35	0.0	0.0	0.0	0.039116941753352015	3.5804981009933195E-4
36-37	0.0	0.0	0.0	0.06301676657748242	3.5804981009933195E-4
38-39	0.0	0.0	0.0	0.10217846455709687	3.5804981009933195E-4
40-41	0.0	0.0	0.0	0.14684517836698852	3.5804981009933195E-4
42-43	0.0	0.0	0.0	0.1882446876597238	3.5804981009933195E-4
44-45	0.0	0.0	0.0	0.23720799919080743	3.5804981009933195E-4
46-47	0.0	0.0	0.0	0.28894619675016087	3.5804981009933195E-4
48-49	0.0	0.0	0.0	0.34457818599434464	3.5804981009933195E-4
50-51	0.0	0.0	0.0	0.4051781163536565	3.5804981009933195E-4
52-53	0.0	0.0	0.0	0.4715515999008202	3.5804981009933195E-4
54-55	0.0	0.0	0.0	0.5401181385348424	3.5804981009933195E-4
56-57	0.0	0.0	0.0	0.6114148069708718	4.028060363617485E-4
58-59	0.0	0.0	0.0	0.6973467613947115	4.4756226262416496E-4
60-61	0.0	0.0	0.0	0.7859193331680336	5.37074715148998E-4
62-63	0.0	0.0	0.0	0.8848305932079741	5.37074715148998E-4
64-65	0.0	0.0	0.0	0.9760437823307789	5.37074715148998E-4
66-67	0.0	0.0	0.0	1.0803705457484718	5.37074715148998E-4
68-69	0.0	8.951245252483299E-5	0.0	1.1988402766650883	5.37074715148998E-4
70-71	0.0	1.342686787872495E-4	0.0	1.3222331924705706	5.37074715148998E-4
72-73	0.0	1.7902490504966597E-4	0.0	1.4468792826114005	7.160996201986639E-4
74-75	0.0	1.7902490504966597E-4	0.0	1.5760457516047346	7.160996201986639E-4
76-77	0.0	1.7902490504966597E-4	0.0	1.7258895971313049	7.160996201986639E-4
78-79	0.0	1.7902490504966597E-4	0.0	1.8990514365405944	7.160996201986639E-4
80-81	0.0	1.7902490504966597E-4	0.0	2.068364240491316	7.608558464610804E-4
82-83	8.951245252483299E-5	1.7902490504966597E-4	0.0	2.240451930470307	9.846369777731629E-4
84-85	8.951245252483299E-5	1.7902490504966597E-4	0.0	2.423236358526016	9.846369777731629E-4
86-87	8.951245252483299E-5	2.68537357574499E-4	0.0	2.6310395170624163	0.001074149430297996
88-89	8.951245252483299E-5	2.68537357574499E-4	0.0	2.87003776530372	0.0012084181090852453
90-91	8.951245252483299E-5	2.68537357574499E-4	0.0	3.1169131093672098	0.0012531743353476619
92-93	8.951245252483299E-5	2.68537357574499E-4	0.0	3.357343556848911	0.0012531743353476619
94-95	8.951245252483299E-5	2.68537357574499E-4	0.0	3.6048007318538122	0.0014321992403973278
96-97	8.951245252483299E-5	2.68537357574499E-4	0.0	3.8911063112544904	0.0014321992403973278
98-99	8.951245252483299E-5	2.68537357574499E-4	0.0	4.199969028691426	0.0014321992403973278
100-101	8.951245252483299E-5	2.68537357574499E-4	0.0	4.525033500035358	0.0014321992403973278
102-103	8.951245252483299E-5	2.68537357574499E-4	0.0	4.877399269399362	0.0014321992403973278
104-105	8.951245252483299E-5	2.68537357574499E-4	0.0	5.245071668145114	0.0014321992403973278
106-107	8.951245252483299E-5	2.68537357574499E-4	0.0	5.630243751359471	0.0014321992403973278
108-109	8.951245252483299E-5	2.68537357574499E-4	0.0	6.0463423869211566	0.0014321992403973278
110-111	8.951245252483299E-5	2.68537357574499E-4	0.0	6.491622082005938	0.0015217116929221609
112-113	8.951245252483299E-5	2.68537357574499E-4	0.0	6.944599848007856	0.0015217116929221609
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1945	0.0	45.92968	1
GGTATCA	1290	0.0	41.08867	1
GTACATG	5570	0.0	38.70572	1
TACATGG	5850	0.0	35.92401	2
TCAACGC	2440	0.0	35.37889	4
ATGGGGG	3150	0.0	35.148758	5
ACATGGG	6040	0.0	34.89252	3
CAACGCA	2460	0.0	34.84456	5
CATGGGG	5170	0.0	33.97015	4
TATCAAC	2690	0.0	33.197468	2
ATCAACG	2665	0.0	33.062107	3
AACGCAG	2605	0.0	32.89619	6
GTATAGG	275	1.8189894E-12	28.153502	1
ACGCAGA	3260	0.0	26.463299	7
TGGGGGG	2805	0.0	25.671034	6
CGCAGAG	3445	0.0	25.212639	8
GAGTACA	3905	0.0	23.029137	1
AGTACAT	3915	0.0	22.201742	2
CCCTAGA	460	0.0	22.009636	1
TACGTCT	225	5.067768E-6	21.159157	6
>>END_MODULE