FastQCFastQC Report
Mon 15 Aug 2016
SRR2921609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117163
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25810.23103163996659395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21540.19280982273849026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.13140428030645482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.059.4790151
GTATCAA33150.049.4727971
TATCAAC43250.037.9111062
TCAACGC43500.037.141634
ATCAACG44500.036.3086133
CAACGCA44650.036.2973675
AACGCAG46900.034.433616
GTACATG56600.030.8722631
TACATGG58500.029.2513472
ACGCAGA55800.029.0483077
ACATGGG58600.028.6914063
CGCAGAG58000.027.9389558
CATGGGG50800.026.523184
GCAGAGT63150.025.6616339
GTATAGG2901.4188117E-1024.6774651
ATGGGGG31250.024.0241585
GCGGTAT1501.6604143E-423.8548811
CGGTATC1501.6629425E-423.8495332
GAGTACT31600.023.54628812-13
AGAGTAC58700.023.34417210-11
Produced by FastQC (version 0.11.5)