FastQCFastQC Report
Mon 15 Aug 2016
SRR2921608_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921608_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845473
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1250.052.3759962
GTACATG45050.037.402071
TACATGG46800.035.6091652
GTATCAA15250.034.3571241
ACATGGG47550.034.171323
GGTATCA11000.033.5585331
CATGGGG39250.033.5121464
GTATCAC3000.031.7543131
ATGGGGG25700.031.4960025
CAACGCA17850.029.3422955
TCAACGC18050.028.6874334
GTAAGTA4200.028.341992
AACGCAG19000.028.1911536
ATCAACG19250.026.5899413
TATCAAC19600.026.4187832
TACCGTA1451.3417337E-424.6150937
GTACTAC2201.5278965E-724.3569981
CTAGTAC1552.1012705E-423.0392953
GAGTACA32600.022.82951
AGTACAT32500.022.3422092
Produced by FastQC (version 0.11.5)