##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921608_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	845473
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.79482017758107	31.0	30.0	33.0	23.0	34.0
2	29.027530151761205	31.0	30.0	34.0	22.0	34.0
3	29.36121673903247	31.0	30.0	34.0	23.0	34.0
4	32.88293653375093	37.0	35.0	37.0	25.0	37.0
5	32.954446800784886	37.0	35.0	37.0	26.0	37.0
6	32.795779403954946	37.0	35.0	37.0	25.0	37.0
7	32.74699369465376	37.0	35.0	37.0	23.0	37.0
8	32.48980511500663	37.0	35.0	37.0	22.0	37.0
9	34.253358770770916	39.0	35.0	39.0	23.0	39.0
10-11	34.29543107822485	39.0	35.0	39.0	23.0	39.0
12-13	34.22905817217108	39.0	35.0	39.0	22.0	39.0
14-15	35.42328081440803	40.0	36.0	41.0	21.5	41.0
16-17	35.37831012935954	40.0	36.0	41.0	20.0	41.0
18-19	35.32528123310857	40.0	36.0	41.0	19.0	41.0
20-21	35.3713122713558	40.0	36.5	41.0	19.0	41.0
22-23	35.29067930022603	40.0	36.0	41.0	19.0	41.0
24-25	35.26676251045273	40.0	36.0	41.0	19.0	41.0
26-27	35.22056706719198	40.0	36.0	41.0	19.0	41.0
28-29	35.154627054914826	40.0	36.0	41.0	18.0	41.0
30-31	35.04952316632228	40.0	36.0	41.0	18.0	41.0
32-33	34.94584983790139	40.0	35.5	41.0	16.5	41.0
34-35	34.81211049909341	40.0	35.0	41.0	16.0	41.0
36-37	34.711063511194325	40.0	35.0	41.0	15.0	41.0
38-39	34.587325083119154	40.0	35.0	41.0	11.0	41.0
40-41	34.47083762580236	40.0	35.0	41.0	9.0	41.0
42-43	34.227628794769316	40.0	35.0	41.0	8.5	41.0
44-45	33.88112866998709	39.0	34.0	41.0	6.5	41.0
46-47	33.61809425020078	39.0	34.0	40.5	2.0	41.0
48-49	33.42355285148077	38.0	33.0	40.0	2.0	41.0
50-51	33.21552255364749	38.0	33.0	40.0	2.0	41.0
52-53	33.00353648194561	38.0	33.0	40.0	2.0	41.0
54-55	32.75830038333572	38.0	33.0	40.0	2.0	41.0
56-57	32.50711968330154	37.5	32.0	40.0	2.0	41.0
58-59	32.52303976590619	37.0	33.0	40.0	2.0	41.0
60-61	32.31163088590647	37.0	32.0	40.0	2.0	41.0
62-63	32.00999381411352	36.5	32.0	40.0	2.0	41.0
64-65	31.718119916307202	36.0	32.0	39.0	2.0	41.0
66-67	31.40568711242109	35.5	31.0	39.0	2.0	41.0
68-69	31.07481847439244	35.0	31.0	38.5	2.0	40.0
70-71	30.71855931531817	35.0	31.0	37.5	2.0	40.0
72-73	30.38024218396093	35.0	31.0	37.0	2.0	39.0
74-75	30.021110076844558	35.0	30.5	36.5	2.0	39.0
76-77	29.73191456143484	35.0	30.0	36.0	2.0	38.5
78-79	29.467919141119822	35.0	30.0	35.5	2.0	37.0
80-81	29.218545122079593	34.5	30.0	35.0	2.0	37.0
82-83	29.0051012864988	34.0	30.0	35.0	2.0	36.5
84-85	28.800459032991	34.0	29.5	35.0	2.0	36.0
86-87	28.640521341308357	34.0	29.0	35.0	2.0	36.0
88-89	28.49182587734913	34.0	29.0	35.0	2.0	35.0
90-91	28.31260667105869	34.0	29.0	35.0	2.0	35.0
92-93	28.243237808895138	34.0	29.0	35.0	2.0	35.0
94-95	28.12534699511398	34.0	29.0	35.0	2.0	35.0
96-97	28.04060922111055	34.0	29.0	35.0	2.0	35.0
98-99	27.958679342805745	34.0	29.0	35.0	2.0	35.0
100-101	27.864734296659975	34.0	28.5	35.0	2.0	35.0
102-103	27.015205098211297	32.5	26.0	34.0	2.0	35.0
104-105	27.926928476722498	34.0	29.0	35.0	2.0	35.0
106-107	28.212991426101127	34.0	29.0	35.0	2.0	35.0
108-109	28.22266530096171	34.0	29.0	35.0	2.0	35.0
110-111	28.176246905578296	34.0	29.0	35.0	2.0	35.0
112-113	28.10049877405902	34.0	29.0	35.0	2.0	35.0
114-115	27.99265973011557	34.0	29.0	35.0	2.0	35.0
116-117	27.886421565206696	34.0	29.0	35.0	2.0	35.0
118-119	27.61727104236327	34.0	29.0	35.0	2.0	35.0
120-121	27.49545934642502	34.0	29.0	35.0	2.0	35.0
122-123	27.244709174627694	34.0	27.5	35.0	2.0	35.0
124-125	26.470896764296434	33.5	25.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	52286.0
3	7831.0
4	3917.0
5	3229.0
6	3374.0
7	3603.0
8	3817.0
9	3790.0
10	4210.0
11	4188.0
12	4040.0
13	4095.0
14	4115.0
15	3683.0
16	3314.0
17	2976.0
18	2745.0
19	2583.0
20	2493.0
21	2644.0
22	3012.0
23	3912.0
24	5415.0
25	6782.0
26	7924.0
27	8991.0
28	10582.0
29	12962.0
30	15525.0
31	19469.0
32	25476.0
33	34580.0
34	53459.0
35	84983.0
36	160085.0
37	220282.0
38	49079.0
39	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.57689782252483	17.635715088524627	12.149613425693143	25.637773663257395
2	17.149491584769784	18.98193931192422	38.45928183095726	25.40928727234874
3	20.037615997444952	23.373729129331604	27.958740692110695	28.62991418111275
4	13.462782788703237	14.705308295135294	34.38734289516487	37.444566020996604
5	14.47735610006542	37.207258256272425	33.13345013231566	15.181935511346497
6	34.44772655967892	33.420667854542444	16.895145297728632	15.23646028805001
7	30.33348166312411	31.002030855752434	20.094583911267225	18.56990356985623
8	27.95254205998985	34.19482795550049	19.507528019284226	18.34510196522544
9	28.28029833370404	15.248430009417577	17.885115022218855	38.586156634659524
10-11	26.84965436117911	24.906704440495865	26.61392294588532	21.62971825243971
12-13	26.393810400027917	22.378093897996518	26.855274154540083	24.372821547435482
14-15	24.079131919857694	23.768858222371296	23.84416392626075	28.30784593151026
16-17	24.826543361793156	26.73131259110615	24.272038221986637	24.17010582511406
18-19	24.7756028150598	26.191179124302227	25.572019796532352	23.46119826410562
20-21	25.398364422401166	25.290580047940974	25.221602780860525	24.089452748797335
22-23	25.300186240892064	24.65313521278081	25.33657065913184	24.71010788719529
24-25	24.89308623322198	24.986970393065235	25.255312263921564	24.864631109791226
26-27	24.631628149147407	25.304767652383823	25.28039097352882	24.783213224939946
28-29	24.7485403506945	25.790115801606028	25.160875008579215	24.300468839120256
30-31	25.353920633756328	25.330377191965415	25.19485451572678	24.120847658551476
32-33	24.88902192586002	25.338258330454373	25.214739024055504	24.557980719630105
34-35	24.759698728530267	25.737470195305796	25.28449800907601	24.218333067087926
36-37	24.943413523384248	25.622628676198783	25.183540092561103	24.250417707855863
38-39	24.920485351381693	25.71483328985685	25.097467184125584	24.267214174635875
40-41	24.74531165783045	25.455818028958653	25.31719562679749	24.481674686413406
42-43	24.703880198659355	25.500966559639217	25.12930840705766	24.665844834643767
44-45	24.547302149124413	25.5090078206977	25.448025069766395	24.49566496041149
46-47	24.481003807697384	25.430354984818308	25.34233488689595	24.746306320588364
48-49	24.458702495047117	25.685964923696886	25.247082249535474	24.608250331720527
50-51	24.54759007528778	25.623873448857022	25.380210086444592	24.448326389410614
52-53	24.726444793844255	25.511411295236137	25.251786730457766	24.51035718046185
54-55	24.452723134218882	25.252875983702367	25.518528859212847	24.775872022865904
56-57	24.618245877221106	25.417542647499005	25.201687562038373	24.76252391324152
58-59	24.498202436736303	25.577391043059894	25.416385212069876	24.50802130813393
60-61	24.886175759888083	25.546081293310735	25.40086185224292	24.166881094558263
62-63	24.749325510976085	25.56717118031586	25.366880030570133	24.316623278137918
64-65	24.500008576943166	25.294884178734033	25.39904967469725	24.806057569625555
66-67	24.564969452029512	25.501876416777485	25.24437193278027	24.68878219841274
68-69	24.411103237201974	25.417767403672446	25.43101765477654	24.74011170434904
70-71	24.295450686133492	25.38371467638746	25.64925748007689	24.67157715740215
72-73	24.437317294485503	25.683352636578842	25.235099333830995	24.644230735104657
74-75	24.392722188477173	25.617598473606606	25.34707292420139	24.64260641371483
76-77	24.31845107652524	25.226729505622323	25.401157291986436	25.053662125866
78-79	24.31274145662787	25.243874521523246	25.6879388070305	24.75544521481839
80-81	24.469376975252082	25.638247091133536	25.19047892265914	24.701897010955236
82-83	24.5845802383765	25.55079238979272	25.37516585143379	24.48946152039699
84-85	24.44052637275609	25.721503175235	25.14867741422252	24.689293037786392
86-87	24.18112420082087	25.7324292975268	25.349970809888084	24.736475691764248
88-89	24.132768468523892	25.468545185241272	25.42086845006838	24.97781789616646
90-91	24.431419244053334	25.33200360352047	25.54489050727891	24.691686645147286
92-93	24.458395680176956	25.46944955376417	25.522087047036628	24.550067719022245
94-95	24.425223451924126	25.332634627122737	25.44625324709122	24.79588867386192
96-97	24.19404160079116	25.318435265149297	25.57330177223605	24.914221361823493
98-99	24.06850812260917	25.445105261539265	25.39388799386812	25.092498621983445
100-101	24.07042243524836	25.477593614262865	25.551228790703394	24.900755159785376
102-103	23.884748360906844	25.38119339465862	25.691388004073634	25.042670240360902
104-105	23.984464682702324	25.490446560531137	25.59820256828342	24.92688618848312
106-107	23.9520097555956	25.28269996280147	25.721808215257074	25.043482066345856
108-109	23.896253377307332	25.325250665997906	25.733070877319165	25.045425079375594
110-111	23.760266551857914	25.623208084554754	25.720965055874643	24.89556030771269
112-113	23.793147701204145	25.653155377557724	25.647241391750107	24.90645552948802
114-115	23.734284090297464	25.75537211003813	25.69841422870571	24.811929570958696
116-117	23.73415943502404	25.54374086618844	25.744766041353586	24.977333657433938
118-119	23.744152245318386	25.431484091794555	25.745657287922715	25.078706374964344
120-121	23.538289580290137	25.594791032153903	25.92117946928613	24.945739918269826
122-123	23.560846022647972	25.55478543092416	25.752547128079083	25.131821418348782
124-125	23.489432836954194	25.488435056156312	25.89614893391096	25.12598317297853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	219.0
1	177.5
2	114.5
3	91.0
4	77.5
5	67.0
6	69.5
7	81.0
8	92.0
9	100.0
10	116.0
11	130.5
12	132.5
13	145.5
14	178.5
15	205.5
16	228.5
17	239.0
18	258.0
19	273.5
20	298.0
21	355.0
22	439.5
23	525.0
24	620.0
25	775.5
26	1006.5
27	1342.0
28	1750.0
29	2346.0
30	3200.0
31	4159.5
32	5348.5
33	6616.0
34	7771.0
35	9222.5
36	11083.5
37	12886.0
38	14690.5
39	16698.0
40	18705.0
41	20533.5
42	22219.5
43	24668.5
44	27352.0
45	29576.5
46	31737.5
47	33802.0
48	36253.0
49	38502.0
50	39804.5
51	40351.5
52	39716.5
53	38507.5
54	37905.0
55	36656.0
56	34089.5
57	31221.5
58	28309.0
59	25260.5
60	21732.5
61	18289.5
62	15311.0
63	11996.5
64	8875.0
65	6718.5
66	5216.5
67	4133.5
68	3213.5
69	2446.5
70	1960.5
71	1524.5
72	1170.0
73	937.5
74	726.0
75	562.5
76	405.5
77	249.0
78	163.5
79	138.5
80	104.5
81	72.0
82	54.0
83	43.0
84	32.5
85	26.5
86	25.5
87	26.0
88	17.5
89	14.0
90	16.0
91	12.0
92	14.0
93	12.0
94	6.5
95	5.5
96	7.0
97	7.0
98	6.0
99	5.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02696715329762157
2	0.011945975802893765
3	0.010408374956976746
4	0.011591144838451375
5	0.017268440269529603
6	0.09793334618609939
7	0.060202986967058676
8	0.0508591049034091
9	0.02897786209612844
10-11	0.05245584424339984
12-13	0.014134100083621832
14-15	0.029746662519086947
16-17	0.036074481384976216
18-19	0.044413009049372364
20-21	0.031579955835372625
22-23	0.03914968307681026
24-25	0.0334132491516583
26-27	0.0476656262234276
28-29	0.048138734176017446
30-31	0.026848876309474103
32-33	0.03087029390648785
34-35	0.045891471401215654
36-37	0.010112682486608088
38-39	0.02229521226579678
40-41	0.04393990109678251
42-43	0.024483336546524848
44-45	0.017978102198414378
46-47	0.02501558299318843
48-49	0.03057460143611919
50-51	0.029510108542792027
52-53	0.02531127546355709
54-55	0.023300566665050215
56-57	0.027203707273916494
58-59	0.01916087207988901
60-61	0.023714536123566332
62-63	0.024069367088008723
64-65	0.021703827325059464
66-67	0.02897786209612844
68-69	0.024365059558377384
70-71	0.04867098062268103
72-73	0.0227091817243129
74-75	0.009639574534018236
76-77	0.016263085870276167
78-79	0.02513385998133589
80-81	0.020047949490994983
82-83	0.025252136969483353
84-85	0.01212339128511496
86-87	0.018037240692488108
88-89	0.023300566665050215
90-91	0.02229521226579678
92-93	0.008042835194027485
94-95	0.011945975802893765
96-97	0.015198592976948998
98-99	0.006505234348110465
100-101	0.01052665194512421
102-103	0.004257971573308669
104-105	0.005263325972562105
106-107	0.0011236313874008986
108-109	0.014784623518432878
110-111	0.0010644928933271672
112-113	0.0023064012688755286
114-115	0.013187884178442126
116-117	0.006682649830331661
118-119	0.2729241501502709
120-121	9.46215905179704E-4
122-123	0.0013010468696220932
124-125	0.3078158616537725
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	845473.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.55633748430306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.89436015152138	62.321801831520574
2	8.037701593547519	11.663723788391085
3	2.518200436579114	5.481342021886604
4	1.1927316305458604	3.4616095485635405
5	0.6407899408195683	2.324668560132559
6	0.3919122147073748	1.7061428948717354
7	0.2857891982457778	1.4515072262102333
8	0.20412547746702692	1.1848477625793683
9	0.14213119829686277	0.9281267271608017
>10	0.6693084380119227	8.024568337286746
>50	0.018393089522454584	0.8997746955260036
>100	0.004556630735167261	0.5518866058706902
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	2.3655397629492603E-4
10-11	0.0	0.0	0.0	0.0	2.3655397629492603E-4
12-13	0.0	0.0	0.0	0.0	3.5483096444238906E-4
14-15	0.0	0.0	0.0	5.913849407373151E-5	4.1396945851612056E-4
16-17	0.0	0.0	0.0	1.1827698814746301E-4	5.913849407373151E-4
18-19	0.0	0.0	0.0	1.1827698814746301E-4	8.279389170322411E-4
20-21	0.0	0.0	0.0	1.7741548222119453E-4	8.279389170322411E-4
22-23	0.0	0.0	0.0	2.3655397629492603E-4	9.462159051797042E-4
24-25	0.0	0.0	0.0	5.322464466635836E-4	0.0010644928933271672
26-27	0.0	0.0	0.0	8.870774111059726E-4	0.0010644928933271672
28-29	0.0	0.0	0.0	0.0013601853636958246	0.0010644928933271672
30-31	0.0	0.0	0.0	0.005559018442930762	0.0010644928933271672
32-33	0.0	0.0	0.0	0.01567170092953885	0.0013010468696220932
34-35	0.0	0.0	0.0	0.026908014803547837	0.0013010468696220932
36-37	0.0	0.0	0.0	0.046246302365658035	0.0013010468696220932
38-39	0.0	0.0	0.0	0.08078318290471724	0.0013601853636958246
40-41	0.0	0.0	0.0	0.12584671538890066	0.0014193238577695562
42-43	0.0	0.0	0.0	0.16582433738274316	0.0014193238577695562
44-45	0.0	0.0	0.0	0.20846319160990356	0.0014193238577695562
46-47	0.0	0.0	0.0	0.25358586258816074	0.0014193238577695562
48-49	0.0	0.0	0.0	0.3032621976100952	0.0014193238577695562
50-51	0.0	0.0	0.0	0.36068567535568846	0.0014193238577695562
52-53	0.0	0.0	0.0	0.4203564158760835	0.0015376008459170192
54-55	0.0	0.0	0.0	0.48848396104902225	0.0016558778340644822
56-57	0.0	0.0	0.0	0.559036184478984	0.0016558778340644822
58-59	0.0	0.0	0.0	0.6344377644229917	0.0016558778340644822
60-61	0.0	0.0	0.0	0.716935963655847	0.0017741548222119452
62-63	0.0	0.0	0.0	0.809251152904942	0.0017741548222119452
64-65	0.0	0.0	0.0	0.8932869529837144	0.0017741548222119452
66-67	0.0	0.0	0.0	0.994946024296459	0.0017741548222119452
68-69	0.0	0.0	0.0	1.106599501107664	0.0017741548222119452
70-71	0.0	0.0	0.0	1.2335107093898918	0.0017741548222119452
72-73	0.0	0.0	0.0	1.3462878175884978	0.0018332933162856766
74-75	0.0	0.0	0.0	1.4619627119967165	0.0018924318103594082
76-77	0.0	0.0	0.0	1.6072068534418014	0.0018924318103594082
78-79	0.0	0.0	0.0	1.7680635573223509	0.0018924318103594082
80-81	0.0	0.0	0.0	1.9262590289695827	0.0019515703044331398
82-83	0.0	0.0	0.0	2.088771610684197	0.0020107087985068712
84-85	0.0	0.0	0.0	2.2707407569490687	0.0020107087985068712
86-87	0.0	0.0	0.0	2.4621720622657377	0.0020107087985068712
88-89	0.0	0.0	0.0	2.688258525109613	0.0020107087985068712
90-91	0.0	0.0	0.0	2.923097485076401	0.0020107087985068712
92-93	0.0	0.0	0.0	3.146404438698811	0.0022472627748017972
94-95	0.0	0.0	0.0	3.381657368124115	0.0022472627748017972
96-97	0.0	0.0	1.1827698814746301E-4	3.651388039594405	0.002306401268875529
98-99	0.0	0.0	1.1827698814746301E-4	3.959203901248177	0.0023655397629492605
100-101	0.0	0.0	1.1827698814746301E-4	4.28588494251147	0.0023655397629492605
102-103	0.0	0.0	1.1827698814746301E-4	4.620313126498422	0.002424678257022992
104-105	0.0	0.0	1.1827698814746301E-4	4.954149925544636	0.0024838167510967232
106-107	0.0	0.0	1.1827698814746301E-4	5.334410442438729	0.0024838167510967232
108-109	0.0	0.0	1.1827698814746301E-4	5.742466051547477	0.0024838167510967232
110-111	0.0	0.0	1.1827698814746301E-4	6.173230842380537	0.0026020937392441865
112-113	0.0	0.0	1.1827698814746301E-4	6.608726712739497	0.0026020937392441865
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	125	0.0	52.375996	2
GTACATG	4505	0.0	37.40207	1
TACATGG	4680	0.0	35.609165	2
GTATCAA	1525	0.0	34.357124	1
ACATGGG	4755	0.0	34.17132	3
GGTATCA	1100	0.0	33.558533	1
CATGGGG	3925	0.0	33.512146	4
GTATCAC	300	0.0	31.754313	1
ATGGGGG	2570	0.0	31.496002	5
CAACGCA	1785	0.0	29.342295	5
TCAACGC	1805	0.0	28.687433	4
GTAAGTA	420	0.0	28.34199	2
AACGCAG	1900	0.0	28.191153	6
ATCAACG	1925	0.0	26.589941	3
TATCAAC	1960	0.0	26.418783	2
TACCGTA	145	1.3417337E-4	24.615093	7
GTACTAC	220	1.5278965E-7	24.356998	1
CTAGTAC	155	2.1012705E-4	23.039295	3
GAGTACA	3260	0.0	22.8295	1
AGTACAT	3250	0.0	22.342209	2
>>END_MODULE