FastQCFastQC Report
Mon 15 Aug 2016
SRR2921608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845473
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.1081051671667812No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21650.044.652921
GGTATCA13850.038.347141
GTACATG47400.037.0136341
ATGGGGG25400.036.8333055
ACATGGG48400.035.4943733
TCAACGC26800.034.94234
TACATGG50100.034.5301482
CAACGCA27550.033.958835
ATCAACG28150.033.4804343
CATGGGG40600.033.349284
TATCAAC29300.032.3718572
AACGCAG29350.032.2975546
TGGGGGG17350.031.2696136
TATCACG1203.6303347E-529.8269022
CCGTTAT850.007106650628.0823751
ACGCAGA34050.027.832857
CGCAGAG37050.025.5761458
GTACCGC1701.3768127E-524.548776
GTATAGT2001.7788425E-623.8700161
GCAGAGT40500.023.1044989
Produced by FastQC (version 0.11.5)