##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921608_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	845473
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76135725209439	33.0	31.0	34.0	30.0	34.0
2	31.93192922778137	33.0	31.0	34.0	30.0	34.0
3	32.0003702069729	34.0	31.0	34.0	30.0	34.0
4	35.40839742960449	37.0	35.0	37.0	33.0	37.0
5	35.28611203432871	37.0	35.0	37.0	33.0	37.0
6	35.31877540737552	37.0	35.0	37.0	33.0	37.0
7	35.20404436333271	37.0	35.0	37.0	33.0	37.0
8	35.238315120648444	37.0	36.0	37.0	33.0	37.0
9	36.950068186683666	39.0	38.0	39.0	34.0	39.0
10-11	36.90583909835087	39.0	38.0	39.0	34.0	39.0
12-13	36.84050643840785	39.0	38.0	39.0	33.5	39.0
14-15	38.27475330377197	41.0	39.0	41.0	34.0	41.0
16-17	38.2868565879691	41.0	39.0	41.0	34.0	41.0
18-19	38.28806597017291	41.0	39.0	41.0	34.0	41.0
20-21	38.22840114350192	41.0	39.0	41.0	34.0	41.0
22-23	38.24402730779103	41.0	39.0	41.0	34.0	41.0
24-25	38.216355223644044	41.0	39.0	41.0	34.0	41.0
26-27	38.14935249262839	41.0	39.0	41.0	34.0	41.0
28-29	38.07639333248962	41.0	39.0	41.0	33.5	41.0
30-31	37.97413163992227	41.0	38.0	41.0	33.0	41.0
32-33	37.86606313862181	41.0	38.0	41.0	33.0	41.0
34-35	37.747470942300936	41.0	38.0	41.0	33.0	41.0
36-37	37.68008026276416	40.5	38.0	41.0	33.0	41.0
38-39	37.59872757616151	40.0	38.0	41.0	33.0	41.0
40-41	37.48566896873112	40.0	38.0	41.0	32.5	41.0
42-43	37.340888473079566	40.0	38.0	41.0	32.0	41.0
44-45	37.20260020130743	40.0	37.5	41.0	32.0	41.0
46-47	37.02581336127824	40.0	37.0	41.0	31.0	41.0
48-49	36.85510477566995	40.0	37.0	41.0	31.0	41.0
50-51	36.621359877843524	40.0	36.5	41.0	30.0	41.0
52-53	36.495526764308266	40.0	36.0	41.0	30.0	41.0
54-55	36.37503858786738	40.0	35.5	41.0	30.0	41.0
56-57	36.18890786577454	39.0	35.0	41.0	30.0	41.0
58-59	35.996863294274334	39.0	35.0	41.0	30.0	41.0
60-61	35.74401607147715	39.0	35.0	41.0	30.0	41.0
62-63	35.388795384358815	38.0	35.0	41.0	29.0	41.0
64-65	35.09876069371819	37.0	35.0	40.0	29.0	41.0
66-67	34.79280355493316	37.0	35.0	39.5	29.0	41.0
68-69	34.41769695779759	36.0	35.0	39.0	28.5	41.0
70-71	34.043049275376035	36.0	35.0	39.0	28.0	41.0
72-73	33.716480597251476	35.0	34.5	37.5	28.0	40.0
74-75	33.3893453723537	35.0	34.0	37.0	28.0	39.0
76-77	33.05806158209665	35.0	34.0	37.0	27.0	39.0
78-79	32.79406202208705	35.0	34.0	36.0	27.0	38.0
80-81	32.54156844748442	35.0	34.0	36.0	27.0	37.0
82-83	32.31839100716404	35.0	34.0	35.0	26.0	37.0
84-85	32.1097734640846	35.0	34.0	35.0	26.0	36.0
86-87	31.983205259068	35.0	34.0	35.0	26.0	36.0
88-89	31.88861205502719	35.0	34.0	35.0	26.0	36.0
90-91	31.786449123744934	35.0	34.0	35.0	25.5	36.0
92-93	31.70571029471077	35.0	34.0	35.0	25.0	35.0
94-95	31.640717681108683	35.0	34.0	35.0	25.0	35.0
96-97	31.574068006902642	35.0	33.0	35.0	25.0	35.0
98-99	31.508330839660168	35.0	33.0	35.0	25.0	35.0
100-101	31.44163976850828	35.0	33.0	35.0	24.5	35.0
102-103	30.76299657115011	34.0	31.5	35.0	23.5	35.0
104-105	31.272691144483623	35.0	33.0	35.0	24.0	35.0
106-107	31.372787185397996	35.0	33.0	35.0	24.0	35.0
108-109	31.365333960989883	35.0	33.0	35.0	24.0	35.0
110-111	31.3064893852317	35.0	33.0	35.0	24.0	35.0
112-113	31.270439742014233	35.0	33.0	35.0	24.0	35.0
114-115	31.14911416449727	35.0	33.0	35.0	23.0	35.0
116-117	31.33758854511025	35.0	33.5	35.0	24.0	35.0
118-119	31.32459818350202	35.0	34.0	35.0	24.0	35.0
120-121	31.249026284695077	35.0	34.0	35.0	23.5	35.0
122-123	31.124349920103896	35.0	34.0	35.0	21.5	35.0
124-125	30.483250204323497	34.5	32.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12701.0
3	3353.0
4	1802.0
5	1357.0
6	1511.0
7	1768.0
8	1884.0
9	2056.0
10	2214.0
11	2378.0
12	2549.0
13	3032.0
14	4108.0
15	3559.0
16	2243.0
17	2036.0
18	1878.0
19	1723.0
20	1633.0
21	1666.0
22	1796.0
23	2348.0
24	3134.0
25	4086.0
26	4976.0
27	6010.0
28	7118.0
29	8586.0
30	10392.0
31	13082.0
32	17130.0
33	23478.0
34	36377.0
35	64460.0
36	148753.0
37	321118.0
38	117013.0
39	165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.21509798261347	17.95878625545991	10.949988879056187	26.876126882870434
2	16.361092285000893	19.674803094657683	37.598600520192036	26.365504100149384
3	18.792756253564253	23.87203945111086	28.41760155608508	28.9176027392398
4	13.173756974801861	15.008470460011136	34.39622797976567	37.42154458542133
5	14.345069581070257	37.77111063526222	32.72552181366257	15.158297970004957
6	34.73445749935199	34.05650182922571	16.705902404209763	14.503138267212535
7	30.945463797357508	31.420592046483282	19.213287023614406	18.420657132544807
8	28.618084357379942	35.25414302534035	17.918695531271634	18.209077086008083
9	28.404109435065084	15.293837858815372	17.749654806034712	38.552397900084834
10-11	27.143005086295407	25.052445948864833	26.260729597860145	21.543819366979616
12-13	27.033980493479582	22.13936221264036	26.91616977288152	23.910487520998533
14-15	24.289458283504235	23.638252887819295	24.077971860589383	27.99431696808709
16-17	24.635759473448783	26.731444205404202	24.622564925459677	24.010231395687335
18-19	24.537882539192353	26.061975357285057	26.26714397358602	23.132998129936574
20-21	25.41414110344167	25.088092788902056	25.979875152835145	23.517890954821134
22-23	25.169998829021807	24.422346995139847	26.031022642460144	24.3766315333782
24-25	24.588981103687534	24.88344681608579	26.052497576675528	24.475074503551152
26-27	24.351968489966822	25.09767045756968	26.161370639130872	24.38899041333262
28-29	24.48652021302706	25.612630221809546	25.9358967155864	23.964952849576992
30-31	25.12341024480973	25.166759812053307	25.903584175338057	23.8062457677989
32-33	24.725141813162082	25.043042239059936	25.973677253293005	24.258138694484973
34-35	24.615582965475802	25.33544821843404	26.031302192005075	24.017666624085088
36-37	24.679108251248643	25.276268825757686	26.08060514479532	23.964017778198347
38-39	24.812230617468526	25.408716284307094	25.90111561626207	23.87793748196231
40-41	24.774305114055387	25.084792674149515	26.06569680209641	24.075205409698686
42-43	24.618438102312464	25.142662485015187	25.868320559898134	24.37057885277421
44-45	24.37661678654692	25.251322704384883	26.05227890758161	24.319781601486586
46-47	24.4160987137967	25.217225708925827	25.88853772145431	24.478137855823164
48-49	24.45073824328496	25.474627211684147	25.727745953944215	24.34688859108667
50-51	24.489478496296094	25.536897708818522	25.76541263384741	24.20821116103797
52-53	24.750492037508398	25.456494895110847	25.516107131705194	24.276905935675558
54-55	24.38715988401148	25.148194081462833	25.899529055384342	24.565116979141347
56-57	24.48457357785361	25.365094947750645	25.623414334123822	24.526917140271923
58-59	24.47883708964889	25.363202498033626	25.849088962878415	24.308871449439064
60-61	24.797302332072892	25.314765379297455	25.889415485569582	23.99851680306007
62-63	24.562532341765603	25.50456111300028	25.774590830462618	24.1583157147715
64-65	24.47814683505377	25.067462277010783	25.844779102809373	24.609611785126067
66-67	24.50960562640607	25.441662467679627	25.616068281443315	24.432663624470987
68-69	24.332693255049413	25.33852492609136	25.805898379539414	24.52288343931981
70-71	24.256244729281715	25.234572836743457	25.986873619855395	24.522308814119434
72-73	24.433171013272236	25.576646255421675	25.560323567713723	24.42985916359236
74-75	24.340764073172274	25.592790410081502	25.56327878315105	24.503166733595172
76-77	24.31829525911522	25.14347321084293	25.650301494818166	24.88793003522368
78-79	24.144546392697574	25.303957254671204	25.9037402162696	24.64775613636162
80-81	24.352403920645603	25.56415166421636	25.515835514558123	24.567608900579913
82-83	24.43284476278513	25.535248227076192	25.575878655186056	24.456028354952625
84-85	24.369128175649383	25.637850298714188	25.424297155266324	24.568724370370106
86-87	24.131758199256513	25.70164866293779	25.592360725889527	24.57423241191617
88-89	23.96559269510089	25.366132046459917	25.621022875121234	25.047252383317957
90-91	24.205482068753838	25.371644378156176	25.747827675996245	24.675045877093744
92-93	24.24258373715068	25.594430573182116	25.552915350342353	24.610070339324853
94-95	24.200699633623813	25.325258666319712	25.57696117713505	24.897080522921428
96-97	23.94117062623749	25.347029198237177	25.713692631957443	24.998107543567897
98-99	23.952752711077256	25.441989718102377	25.447844474027058	25.157413096793306
100-101	23.94724609774647	25.54368974526685	25.603064793316875	24.905999363669803
102-103	23.784462768470874	25.328770922794053	25.743515804127814	25.143250504607256
104-105	23.963213491146377	25.4701805971332	25.530915830546924	25.035690081173495
106-107	23.998379594721264	25.296714539589043	25.641967313941695	25.062938551747997
108-109	23.887912746902977	25.312836914371484	25.64082850699954	25.158421831726002
110-111	23.77893794361263	25.73849194474572	25.491766147470113	24.990803964171533
112-113	23.77770720563284	25.634365717077056	25.577178624209836	25.010748453080268
114-115	23.74540641747282	25.74671219542197	25.456637881990318	25.05124350511489
116-117	23.659762507000657	25.54726351976713	25.573521523579274	25.21945244965294
118-119	23.675470251972026	25.46167666724624	25.548553375653942	25.314299705127794
120-121	23.494285856222632	25.60376416707868	25.73328160521192	25.168668371486763
122-123	23.503317418948114	25.66746090744522	25.482473181806625	25.346748491800042
124-125	23.30301833641891	25.684958879081233	25.44819758881213	25.563825195687734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	6.0
2	15.0
3	30.0
4	26.5
5	23.5
6	58.0
7	183.5
8	290.0
9	310.5
10	287.0
11	221.5
12	204.5
13	236.5
14	250.0
15	272.5
16	322.5
17	350.0
18	354.5
19	382.5
20	415.0
21	460.0
22	509.5
23	548.0
24	629.0
25	767.5
26	989.5
27	1277.0
28	1638.5
29	2254.5
30	3035.0
31	3995.5
32	5290.5
33	6501.0
34	7763.0
35	9277.0
36	11115.0
37	12975.0
38	14704.5
39	16824.0
40	18924.0
41	20819.5
42	22561.5
43	24906.0
44	27738.0
45	30291.5
46	32487.0
47	34611.0
48	37167.5
49	39198.5
50	40490.0
51	41293.0
52	40597.0
53	39390.5
54	38754.0
55	37194.0
56	34393.0
57	30941.0
58	27874.5
59	24817.0
60	21006.5
61	17577.0
62	14460.0
63	11095.5
64	8010.0
65	5839.5
66	4419.0
67	3417.5
68	2616.0
69	1933.5
70	1503.0
71	1176.0
72	904.5
73	717.5
74	533.0
75	392.5
76	276.0
77	152.5
78	73.5
79	47.5
80	28.0
81	15.5
82	9.5
83	5.5
84	4.0
85	5.0
86	4.0
87	1.5
88	0.5
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02613921438058933
2	0.001892431810359408
3	0.032526171740552334
4	0.1612115348449921
5	0.023655397629492602
6	0.06860065312552854
7	0.0518053208085888
8	0.04470870151974102
9	0.035009988491649054
10-11	0.0657028669159157
12-13	0.056004153887823746
14-15	0.0593159095559527
16-17	0.050504273938966705
18-19	0.03719811277237712
20-21	0.023596259135418872
22-23	0.0030752016918340385
24-25	0.005145048984414641
26-27	0.003311755668128964
28-29	0.004790218019972253
30-31	0.002424678257022992
32-33	0.0097578515221657
34-35	0.004376248561456132
36-37	0.006564372842184198
38-39	0.0024838167510967232
40-41	0.0033708941622026963
42-43	0.0039622791029400115
44-45	0.0054407414547832985
46-47	0.004612802537751057
48-49	0.0023655397629492605
50-51	0.001655877834064482
52-53	0.0010644928933271672
54-55	0.005263325972562105
56-57	9.46215905179704E-4
58-59	9.46215905179704E-4
60-61	2.36553976294926E-4
62-63	0.0012419083755483616
64-65	5.91384940737315E-5
66-67	0.0036665866325713533
68-69	4.139694585161205E-4
70-71	0.0
72-73	0.0028386477155391125
74-75	0.005145048984414641
76-77	0.0022472627748017972
78-79	5.91384940737315E-5
80-81	0.0
82-83	0.005618156937004494
84-85	0.001892431810359408
86-87	0.0
88-89	0.0015376008459170192
90-91	8.870774111059726E-4
92-93	0.0
94-95	0.003607448138497622
96-97	0.0013010468696220932
98-99	7.688004229585096E-4
100-101	0.0
102-103	0.0015967393399907506
104-105	0.0
106-107	6.505234348110466E-4
108-109	0.002897786209612844
110-111	0.0
112-113	2.9569247036865756E-4
114-115	0.0
116-117	0.0019515703044331398
118-119	0.0026020937392441865
120-121	0.0031934786799815013
122-123	0.0013601853636958246
124-125	0.11254055422231107
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	845473.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.54864576691473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.3674156386712	57.1472344311842
2	9.499196296934254	13.023140840578687
3	2.8729439057156196	5.908092423033494
4	1.3867985403653442	3.802526475743135
5	0.7901457232779648	2.708170964461192
6	0.4775681329701521	1.964198926592267
7	0.34315933337243276	1.6466175309470243
8	0.23239261201813563	1.2744159072063383
9	0.17801733315849713	1.0982562399947395
>10	0.8239641322123484	9.4982303107294
>50	0.021819049349516662	1.0034932295560939
>100	0.00605988341741768	0.6443397652593762
>500	5.194185371167386E-4	0.2812829547141164
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	914	0.1081051671667812	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	5.913849407373151E-5
14-15	5.913849407373151E-5	0.0	0.0	5.913849407373151E-5	1.1827698814746301E-4
16-17	1.1827698814746301E-4	0.0	0.0	1.1827698814746301E-4	1.1827698814746301E-4
18-19	1.1827698814746301E-4	0.0	0.0	1.1827698814746301E-4	2.3655397629492603E-4
20-21	1.1827698814746301E-4	0.0	0.0	1.7741548222119453E-4	2.9569247036865756E-4
22-23	1.1827698814746301E-4	0.0	0.0	2.3655397629492603E-4	4.1396945851612056E-4
24-25	1.1827698814746301E-4	0.0	0.0	5.322464466635836E-4	4.7310795258985206E-4
26-27	1.1827698814746301E-4	0.0	0.0	8.870774111059726E-4	4.7310795258985206E-4
28-29	1.1827698814746301E-4	0.0	0.0	0.0014784623518432878	4.7310795258985206E-4
30-31	1.1827698814746301E-4	0.0	0.0	0.005795572419225688	4.7310795258985206E-4
32-33	1.1827698814746301E-4	0.0	0.0	0.015967393399907506	4.7310795258985206E-4
34-35	1.1827698814746301E-4	0.0	0.0	0.027381122756137688	4.7310795258985206E-4
36-37	1.1827698814746301E-4	0.0	0.0	0.04742907224713267	4.7310795258985206E-4
38-39	1.1827698814746301E-4	0.0	0.0	0.08326699965581397	4.7310795258985206E-4
40-41	1.1827698814746301E-4	0.0	0.0	0.1288036400925872	4.7310795258985206E-4
42-43	1.1827698814746301E-4	0.0	0.0	0.16842643112198735	5.322464466635836E-4
44-45	1.1827698814746301E-4	0.0	0.0	0.21153839330173763	6.505234348110466E-4
46-47	1.1827698814746301E-4	0.0	0.0	0.2564836487977736	8.279389170322411E-4
48-49	1.1827698814746301E-4	0.0	0.0	0.3068105072545191	9.462159051797041E-4
50-51	1.1827698814746301E-4	0.0	0.0	0.36500278542307085	9.462159051797041E-4
52-53	1.1827698814746301E-4	0.0	0.0	0.42597457281308804	9.462159051797041E-4
54-55	1.1827698814746301E-4	0.0	0.0	0.49433867196232173	9.462159051797041E-4
56-57	1.1827698814746301E-4	0.0	0.0	0.5657188343093156	9.462159051797041E-4
58-59	1.1827698814746301E-4	0.0	0.0	0.6421257686525768	9.462159051797041E-4
60-61	1.1827698814746301E-4	0.0	0.0	0.725097075838022	9.462159051797041E-4
62-63	1.1827698814746301E-4	0.0	0.0	0.8190681429211815	0.0010644928933271672
64-65	1.1827698814746301E-4	0.0	0.0	0.9061200061977142	0.0010644928933271672
66-67	1.1827698814746301E-4	0.0	0.0	1.0089027088978595	0.0010644928933271672
68-69	1.1827698814746301E-4	0.0	0.0	1.1236313874008987	0.0010644928933271672
70-71	1.1827698814746301E-4	0.0	0.0	1.253736074363108	0.0013010468696220932
72-73	1.1827698814746301E-4	0.0	0.0	1.368464752866147	0.0014784623518432878
74-75	1.1827698814746301E-4	0.0	0.0	1.4891072807765595	0.0015376008459170192
76-77	1.1827698814746301E-4	0.0	0.0	1.6386093937949526	0.0015376008459170192
78-79	1.1827698814746301E-4	0.0	0.0	1.8033100997902949	0.0015376008459170192
80-81	1.1827698814746301E-4	0.0	0.0	1.9648173271056557	0.0015376008459170192
82-83	1.1827698814746301E-4	0.0	0.0	2.1309373569587677	0.0015376008459170192
84-85	1.1827698814746301E-4	0.0	0.0	2.3208310614295193	0.0015376008459170192
86-87	1.1827698814746301E-4	0.0	0.0	2.519536401517257	0.0015376008459170192
88-89	1.1827698814746301E-4	0.0	0.0	2.7517141292507272	0.0016558778340644822
90-91	1.1827698814746301E-4	0.0	0.0	2.993827123988584	0.0016558778340644822
92-93	1.1827698814746301E-4	0.0	0.0	3.223757588947252	0.0017741548222119452
94-95	1.1827698814746301E-4	0.0	0.0	3.464096428862897	0.0017741548222119452
96-97	1.1827698814746301E-4	0.0	0.0	3.740332334681297	0.0017741548222119452
98-99	1.1827698814746301E-4	0.0	0.0	4.057078108940202	0.0017741548222119452
100-101	1.1827698814746301E-4	0.0	0.0	4.3890224761760575	0.0017741548222119452
102-103	1.1827698814746301E-4	0.0	0.0	4.729423648064456	0.0018924318103594082
104-105	1.1827698814746301E-4	0.0	0.0	5.069351712000265	0.0018924318103594082
106-107	1.1827698814746301E-4	0.0	0.0	5.4548164163728465	0.0020107087985068712
108-109	1.1827698814746301E-4	0.0	0.0	5.870027783264516	0.0021289857866543345
110-111	1.1827698814746301E-4	0.0	0.0	6.307711777904203	0.0022472627748017972
112-113	1.1827698814746301E-4	0.0	0.0	6.754621377619392	0.0023655397629492605
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2165	0.0	44.65292	1
GGTATCA	1385	0.0	38.34714	1
GTACATG	4740	0.0	37.013634	1
ATGGGGG	2540	0.0	36.833305	5
ACATGGG	4840	0.0	35.494373	3
TCAACGC	2680	0.0	34.9423	4
TACATGG	5010	0.0	34.530148	2
CAACGCA	2755	0.0	33.95883	5
ATCAACG	2815	0.0	33.480434	3
CATGGGG	4060	0.0	33.34928	4
TATCAAC	2930	0.0	32.371857	2
AACGCAG	2935	0.0	32.297554	6
TGGGGGG	1735	0.0	31.269613	6
TATCACG	120	3.6303347E-5	29.826902	2
CCGTTAT	85	0.0071066506	28.082375	1
ACGCAGA	3405	0.0	27.83285	7
CGCAGAG	3705	0.0	25.576145	8
GTACCGC	170	1.3768127E-5	24.54877	6
GTATAGT	200	1.7788425E-6	23.870016	1
GCAGAGT	4050	0.0	23.104498	9
>>END_MODULE