FastQCFastQC Report
Mon 15 Aug 2016
SRR2921607_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921607_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187800
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24300.043.1013031
AGCCTCG8550.042.451482
TCAACGC29250.036.2095574
CAACGCA29400.036.024825
GTACATG64050.035.770551
GGTATCA15650.035.3632851
TACATGG66750.033.7844662
ATCAACG31350.033.784043
AACGCAG32000.033.2809376
ACATGGG68450.032.771553
TATCAAC32500.032.405522
CATGGGG57750.031.2190534
ATGGGGG34950.030.3041365
TACGGTC850.00720947828.0007845
ACGCAGA38400.027.2635567
CCTCGGT7700.027.0462114
CGGTCTC5400.026.4373867
GTATAGG2308.265488E-925.8735581
TCGGTCT4650.025.5899546
CGCAGAG41750.025.3608938
Produced by FastQC (version 0.11.5)