##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921607_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1187800
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.70377841387439	31.0	30.0	34.0	16.0	34.0
2	28.643034180838526	31.0	30.0	34.0	16.0	34.0
3	28.76445697928944	31.0	30.0	34.0	16.0	34.0
4	32.3281208957737	37.0	33.0	37.0	19.0	37.0
5	32.16581916147499	35.0	33.0	37.0	19.0	37.0
6	32.35495874726385	37.0	35.0	37.0	18.0	37.0
7	32.03892827075265	36.0	33.0	37.0	17.0	37.0
8	32.07794999158107	36.0	33.0	37.0	17.0	37.0
9	33.72280686984341	39.0	35.0	39.0	17.0	39.0
10-11	33.813961104563056	39.0	35.0	39.0	17.0	39.0
12-13	33.75340040410843	39.0	35.0	39.0	16.5	39.0
14-15	34.88253241286412	40.0	36.0	41.0	16.0	41.0
16-17	34.803336841219064	40.0	36.0	41.0	14.5	41.0
18-19	34.701634955379696	40.0	36.0	41.0	12.5	41.0
20-21	34.80283170567436	40.0	36.0	41.0	13.0	41.0
22-23	34.69752357299208	40.0	36.0	41.0	12.5	41.0
24-25	34.69591934669136	40.0	35.5	41.0	12.5	41.0
26-27	34.6899536959084	40.0	35.5	41.0	10.5	41.0
28-29	34.62832000336757	40.0	35.0	41.0	10.0	41.0
30-31	34.511807964303756	40.0	35.0	41.0	9.0	41.0
32-33	34.428505219733964	40.0	35.0	41.0	8.0	41.0
34-35	34.269959168210136	40.0	35.0	41.0	7.5	41.0
36-37	34.14707358141101	40.0	35.0	41.0	2.0	41.0
38-39	34.004173261491836	40.0	35.0	41.0	2.0	41.0
40-41	33.883885755177644	40.0	34.0	41.0	2.0	41.0
42-43	33.54899856878262	39.0	33.5	41.0	2.0	41.0
44-45	33.0403072907897	38.0	33.0	40.0	2.0	41.0
46-47	32.71154697760566	38.0	32.5	40.0	2.0	41.0
48-49	32.47496211483415	38.0	32.0	40.0	2.0	41.0
50-51	32.238632766459006	38.0	32.0	40.0	2.0	41.0
52-53	32.015353173935004	37.5	31.0	40.0	2.0	41.0
54-55	31.769970533759892	37.0	31.0	40.0	2.0	41.0
56-57	31.504108435763598	37.0	31.0	40.0	2.0	41.0
58-59	31.51412022225964	36.5	31.0	40.0	2.0	41.0
60-61	31.309080653308637	36.0	31.0	39.5	2.0	41.0
62-63	31.010858730425998	36.0	30.5	39.0	2.0	41.0
64-65	30.716264101700624	35.0	30.0	39.0	2.0	40.5
66-67	30.405645731604647	35.0	30.0	38.5	2.0	40.0
68-69	30.086564236403433	35.0	30.0	37.5	2.0	40.0
70-71	29.743626873210978	35.0	29.5	37.0	2.0	39.5
72-73	29.418622663748103	35.0	29.0	36.5	2.0	39.0
74-75	29.073450496716617	34.5	29.0	36.0	2.0	39.0
76-77	28.79097617444014	34.0	29.0	35.5	2.0	37.5
78-79	28.5353864286917	34.0	29.0	35.0	2.0	37.0
80-81	28.293368412190603	34.0	29.0	35.0	2.0	37.0
82-83	28.08215061458158	34.0	28.0	35.0	2.0	36.0
84-85	27.883274541168547	34.0	28.0	35.0	2.0	36.0
86-87	27.720711820171744	34.0	27.0	35.0	2.0	35.5
88-89	27.5597225963967	34.0	27.0	35.0	2.0	35.0
90-91	27.38596522983667	34.0	27.0	35.0	2.0	35.0
92-93	27.30879988213504	34.0	27.0	35.0	2.0	35.0
94-95	27.186458157939047	34.0	26.0	35.0	2.0	35.0
96-97	27.104816888365043	34.0	26.0	35.0	2.0	35.0
98-99	27.009363529213672	34.0	25.0	35.0	2.0	35.0
100-101	26.898655076612222	34.0	25.0	35.0	2.0	35.0
102-103	25.9806373126789	31.5	24.5	34.0	2.0	35.0
104-105	26.941980131335242	33.5	26.0	35.0	2.0	35.0
106-107	27.256594544536117	34.0	27.0	35.0	2.0	35.0
108-109	27.267764354268394	34.0	27.0	35.0	2.0	35.0
110-111	27.205095554807208	34.0	27.0	35.0	2.0	35.0
112-113	27.12427639333221	34.0	27.0	35.0	2.0	35.0
114-115	27.010774962114834	34.0	27.0	35.0	2.0	35.0
116-117	26.896839114329012	34.0	25.5	35.0	2.0	35.0
118-119	26.602369085704662	34.0	25.0	35.0	2.0	35.0
120-121	26.456196750294662	34.0	25.0	35.0	2.0	35.0
122-123	26.176536874894765	34.0	24.5	35.0	2.0	35.0
124-125	25.34804259976427	33.0	21.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83922.0
3	11580.0
4	6100.0
5	5104.0
6	5124.0
7	5474.0
8	5991.0
9	6165.0
10	6380.0
11	6588.0
12	6593.0
13	6678.0
14	6806.0
15	6133.0
16	5315.0
17	4786.0
18	4427.0
19	4063.0
20	4056.0
21	4259.0
22	4859.0
23	6176.0
24	8594.0
25	10701.0
26	12163.0
27	14225.0
28	17079.0
29	20754.0
30	24981.0
31	31512.0
32	41524.0
33	57099.0
34	85155.0
35	132441.0
36	225108.0
37	252645.0
38	47223.0
39	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.681144897392066	17.5478514227009	11.859995115912861	25.91100856399417
2	16.585612695611466	19.933046276541106	37.7022209573911	25.779120070456337
3	21.035920777774127	23.067583735582392	27.55307119576794	28.343424290875536
4	13.743493628765949	14.515417426550204	33.521178227842135	38.21991071684172
5	14.803815406793161	37.17226083398029	32.0375612787406	15.986362480485953
6	34.909005720218296	33.21493458996114	16.966834868724867	14.909224821095707
7	30.293697433685573	31.077558901229242	18.722790193012724	19.90595347207246
8	29.107345318056772	34.14500605637908	18.070163025258218	18.677485600305936
9	28.38309203479011	15.699254882674337	17.622260548530313	38.29539253400524
10-11	27.02376464890021	25.154054310172363	25.785096816990094	22.037084223937327
12-13	26.45986868833583	22.625391272131743	26.254375827539246	24.660364211993187
14-15	24.390923786468885	23.941593360807154	23.26736613039804	28.400116722325926
16-17	25.26321153018521	26.925438348999837	23.82214893127823	23.989201189536725
18-19	25.07875196248206	26.24078559925611	25.102672344669124	23.577790093592707
20-21	25.45796160293614	25.134441938052486	25.210321468980556	24.197274990030813
22-23	25.781908653024168	24.6524312337291	24.948097352101666	24.617562761145066
24-25	25.199849418137532	25.164688384815825	24.93864715503026	24.696815042016382
26-27	24.753943847395913	25.412072986761608	25.216870544772746	24.617112621069733
28-29	24.982206465643948	25.524351241420145	25.186968984079734	24.30647330885617
30-31	25.43285376953316	25.178573233131335	25.131961450750673	24.256611546584832
32-33	24.75744651106581	25.358666721126543	25.251838336694266	24.632048431113375
34-35	24.76995909401218	25.81158145252686	25.184746568601234	24.23371288485973
36-37	25.136347236997704	25.624818712376896	24.99102244745974	24.247811603165655
38-39	25.122376164920883	25.708678623728133	24.989031828328955	24.17991338302203
40-41	24.90073816775376	25.526294067705248	25.112569740175733	24.46039802436526
42-43	24.998210536864203	25.340019048308772	25.041536597257952	24.620233817569076
44-45	24.690063246212258	25.384163269705397	25.37136414274058	24.55440934134177
46-47	24.660955182378302	25.395950536025342	25.360539726423077	24.58255455517328
48-49	24.67294693883051	25.57199546580744	25.213614031068985	24.541443564293065
50-51	24.622664032981977	25.601916397213664	25.26627739672816	24.5091421730762
52-53	24.830881789465618	25.539397195580904	25.10696995241195	24.522751062541527
54-55	24.669665661481304	25.242840264789574	25.203935296490382	24.88355877723874
56-57	24.756901783015	25.465796141923715	25.00048421745827	24.776817857603014
58-59	24.644240675566948	25.577915491819187	25.19844168595332	24.57940214666054
60-61	24.85339021444944	25.484250184841173	25.335029886164783	24.3273297145446
62-63	24.68943524034243	25.525930641659805	25.30336484454078	24.481269273456988
64-65	24.640454519043974	25.2843901810548	25.256264836329862	24.818890463571364
66-67	24.624707195608703	25.4386434003585	25.128059155855944	24.808590248176856
68-69	24.535180904501456	25.426124521580963	25.30014610464798	24.738548469269602
70-71	24.382987719979482	25.403276412909896	25.492982303933665	24.720753563176952
72-73	24.530932240855904	25.581276868081666	25.17959773139708	24.708193159665353
74-75	24.419074594543623	25.643021569892078	25.198034140681703	24.739869694882604
76-77	24.500734678067857	25.31519583696462	25.32631073724628	24.857758747721235
78-79	24.400030990047817	25.360596154202437	25.387586127911423	24.851786727838327
80-81	24.464918843656307	25.570005898681618	25.286397623349178	24.678677634312894
82-83	24.55918223499249	25.510524172817995	25.308794544415676	24.62149904777384
84-85	24.546373089714983	25.568896560434474	25.079400496779353	24.80532985307119
86-87	24.411849653791453	25.66166124527295	25.15184253005484	24.77464657088076
88-89	24.32376558245443	25.450110672426042	25.261354923544026	24.964768821575504
90-91	24.544736858725376	25.384717406544272	25.27065689005097	24.799888844679387
92-93	24.489484906720776	25.497106796974816	25.360582134760744	24.652826161543658
94-95	24.45088856443755	25.397045921090967	25.3605030455203	24.791562468951188
96-97	24.35206590762979	25.367305447155463	25.3443600399629	24.936268605251847
98-99	24.26221005885649	25.393748271372157	25.24000826794542	25.104033401825937
100-101	24.281230726483944	25.459585870684048	25.410035173837908	24.849148228994103
102-103	24.012212946093307	25.404754848638945	25.57183461923156	25.01119758603619
104-105	24.04148358194127	25.549759334269	25.437993107959862	24.97076397582987
106-107	24.079392855383887	25.401596831761857	25.523503954193906	24.995506358660354
108-109	24.077580958748275	25.441790283181952	25.46490391213958	25.015724845930194
110-111	23.88269737936028	25.683742604390957	25.51317265576821	24.920387360480557
112-113	23.832423856292102	25.754133479489518	25.59366476941463	24.819777894803753
114-115	23.858019753626973	25.73524582572013	25.495019492602918	24.91171492804998
116-117	23.672015565945202	25.72220243643078	25.578523912602368	25.027258085021653
118-119	23.809616269400745	25.491682530570554	25.608815124667068	25.089886075361633
120-121	23.619480436930374	25.73000282458169	25.651327069300756	24.99918966918718
122-123	23.630125165117434	25.698628799174266	25.59044478363618	25.08080125207212
124-125	23.470305482883198	25.581974702700144	25.689899489764034	25.257820324652624
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	381.0
1	282.5
2	171.5
3	146.5
4	125.5
5	115.5
6	121.0
7	157.0
8	197.5
9	218.0
10	213.5
11	224.5
12	254.0
13	272.0
14	304.0
15	345.5
16	377.0
17	411.0
18	443.5
19	482.5
20	545.0
21	593.5
22	693.0
23	851.5
24	1017.0
25	1294.0
26	1719.0
27	2370.0
28	3035.5
29	3808.0
30	4925.5
31	6173.0
32	7765.5
33	9482.0
34	11316.5
35	13135.0
36	15256.0
37	17633.5
38	19868.5
39	22253.0
40	24787.0
41	27150.5
42	29570.5
43	33038.0
44	36420.5
45	39637.0
46	42906.5
47	45654.0
48	49055.0
49	52276.0
50	54005.5
51	54914.5
52	54425.5
53	53426.5
54	53261.0
55	51755.5
56	48699.5
57	45131.0
58	41388.0
59	37389.0
60	32322.0
61	27474.0
62	23179.0
63	18385.5
64	13918.5
65	10762.5
66	8315.0
67	6381.0
68	5103.5
69	4090.0
70	3274.0
71	2585.5
72	1986.0
73	1492.0
74	1172.5
75	973.0
76	720.5
77	469.0
78	319.5
79	238.5
80	175.0
81	136.0
82	113.5
83	95.0
84	71.0
85	52.0
86	49.5
87	44.0
88	37.0
89	30.5
90	29.5
91	24.0
92	21.5
93	18.5
94	14.0
95	12.5
96	7.0
97	9.0
98	8.0
99	3.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022731099511702307
2	0.009597575349385419
3	0.008671493517427176
4	0.009934332379188416
5	0.016332715945445362
6	0.09530223943424819
7	0.06128977942414548
8	0.05236571813436605
9	0.028287590503451757
10-11	0.05367065162485266
12-13	0.014101700623000505
14-15	0.030855362855699616
16-17	0.03763259808048493
18-19	0.04445192793399562
20-21	0.03296009429196835
22-23	0.0404950328338104
24-25	0.034180838525004205
26-27	0.04807206600437784
28-29	0.04807206600437784
30-31	0.027950833473648764
32-33	0.032118201717460854
34-35	0.047609025088398725
36-37	0.009723859235561542
38-39	0.022983667284054553
40-41	0.04512544199360162
42-43	0.02466745243306954
44-45	0.018437447381714094
46-47	0.026056575181006906
48-49	0.03182353931638323
50-51	0.030602795083347366
52-53	0.026687994611887523
54-55	0.024499073918168045
56-57	0.026687994611887523
58-59	0.019742380872200708
60-61	0.024751641690520288
62-63	0.024078127630914298
64-65	0.021805017679744063
66-67	0.03035022731099512
68-69	0.024835830947971038
70-71	0.04941909412358983
72-73	0.023993938373463544
74-75	0.01060784643879441
76-77	0.017258797777403603
78-79	0.02710894089914127
80-81	0.0207526519616097
82-83	0.0268984677555144
84-85	0.012628388617612393
86-87	0.01860582589661559
88-89	0.02412022225963967
90-91	0.02294157265532918
92-93	0.008629398888701801
94-95	0.012965145647415391
96-97	0.016711567603973732
98-99	0.0067772352247853175
100-101	0.010734130324970533
102-103	0.0037043273278329686
104-105	0.004462030644889712
106-107	8.839872032328674E-4
108-109	0.015911769658191615
110-111	0.0014733120053881125
112-113	0.002483583094797104
114-115	0.014312173766627379
116-117	0.006650951338609193
118-119	0.2731941404276814
120-121	0.0011365549755851154
122-123	7.577033170567436E-4
124-125	0.3066172756356289
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1187800.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.20834208902771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.65436770741897	58.58788439625392
2	8.534638990296028	11.81336429693523
3	2.743259698473936	5.695693669530816
4	1.2760169583538203	3.5324407266060733
5	0.7302161801234642	2.52685255964639
6	0.45786162302735406	1.901270630154874
7	0.3135805916914374	1.5191675003582508
8	0.23327409659126383	1.2915610781917666
9	0.16864093287852386	1.0504223435582676
>10	0.8570735307422248	10.164968377791732
>50	0.023924267864805584	1.0897697244424869
>100	0.007024314871319105	0.7828524269689522
>500	1.21107666828687E-4	0.04375226956122524
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	8.418925745074928E-5
9	0.0	0.0	0.0	0.0	1.6837851490149856E-4
10-11	0.0	0.0	0.0	0.0	1.6837851490149856E-4
12-13	0.0	0.0	0.0	0.0	1.6837851490149856E-4
14-15	0.0	0.0	0.0	0.0	2.946624010776225E-4
16-17	0.0	0.0	0.0	0.0	4.209462872537464E-4
18-19	0.0	0.0	0.0	0.0	4.209462872537464E-4
20-21	0.0	0.0	0.0	0.0	4.209462872537464E-4
22-23	0.0	0.0	0.0	8.418925745074928E-5	5.051355447044957E-4
24-25	0.0	0.0	0.0	2.5256777235224784E-4	5.89324802155245E-4
26-27	0.0	0.0	0.0	6.314194308806196E-4	6.314194308806196E-4
28-29	0.0	0.0	0.0	0.0024414884660717293	7.997979457821182E-4
30-31	0.0	0.0	0.0	0.010523657181343661	8.418925745074928E-4
32-33	0.0	0.0	0.0	0.02576191277992928	9.260818319582421E-4
34-35	0.0	0.0	0.0	0.042810237413706015	9.260818319582421E-4
36-37	0.0	0.0	0.0	0.06726721670314867	9.260818319582421E-4
38-39	0.0	0.0	0.0	0.10641522141774709	9.260818319582421E-4
40-41	0.0	0.0	0.0	0.15364539484761744	9.260818319582421E-4
42-43	0.0	0.0	0.0	0.19700286243475332	9.260818319582421E-4
44-45	0.0	0.0	0.0	0.24717965987539992	9.681764606836168E-4
46-47	0.0	0.0	0.0	0.30249200202054216	0.0010102710894089914
48-49	0.0	0.0	0.0	0.3631924566425324	0.0010102710894089914
50-51	0.0	0.0	0.0	0.42401919515069875	0.0010944603468597406
52-53	0.0	0.0	0.0	0.4893500589324802	0.00117864960431049
54-55	0.0	0.0	0.0	0.5595638996464051	0.00117864960431049
56-57	0.0	0.0	0.0	0.6437952517258798	0.00117864960431049
58-59	0.0	0.0	0.0	0.7275635628893753	0.00117864960431049
60-61	0.0	0.0	0.0	0.8140259302912949	0.00117864960431049
62-63	0.0	0.0	0.0	0.9113066172756357	0.0012628388617612393
64-65	0.0	0.0	0.0	1.0087977774036032	0.0012628388617612393
66-67	0.0	0.0	0.0	1.1208115844418252	0.0012628388617612393
68-69	0.0	0.0	0.0	1.2361929617780771	0.0012628388617612393
70-71	0.0	0.0	0.0	1.3590250883987203	0.0012628388617612393
72-73	0.0	0.0	0.0	1.4754167368243811	0.0013470281192119885
74-75	0.0	0.0	0.0	1.6013638659707021	0.0013470281192119885
76-77	0.0	0.0	0.0	1.7527782454958747	0.0013470281192119885
78-79	4.209462872537464E-5	0.0	0.0	1.9205253409664926	0.0013470281192119885
80-81	8.418925745074928E-5	0.0	0.0	2.087851490149857	0.001389122747937363
82-83	8.418925745074928E-5	0.0	0.0	2.2622074423303586	0.0015995958915642364
84-85	8.418925745074928E-5	0.0	0.0	2.4396783970365385	0.0015995958915642364
86-87	8.418925745074928E-5	0.0	0.0	2.645436942246169	0.0015995958915642364
88-89	8.418925745074928E-5	0.0	0.0	2.879062131671999	0.0015995958915642364
90-91	8.418925745074928E-5	0.0	0.0	3.120432732783297	0.0015995958915642364
92-93	8.418925745074928E-5	0.0	0.0	3.352458326317562	0.0015995958915642364
94-95	8.418925745074928E-5	0.0	0.0	3.595091766290621	0.0016837851490149856
96-97	8.418925745074928E-5	0.0	0.0	3.870643205926924	0.0016837851490149856
98-99	8.418925745074928E-5	0.0	0.0	4.187699949486445	0.0016837851490149856
100-101	8.418925745074928E-5	0.0	0.0	4.524625357804345	0.0016837851490149856
102-103	8.418925745074928E-5	0.0	0.0	4.867444014143795	0.001767974406465735
104-105	8.418925745074928E-5	0.0	0.0	5.220702138407139	0.0019363529213672335
106-107	8.418925745074928E-5	4.209462872537464E-5	0.0	5.603510692035696	0.0020205421788179827
108-109	8.418925745074928E-5	8.418925745074928E-5	0.0	6.019531907728574	0.0020626368075433573
110-111	8.418925745074928E-5	8.418925745074928E-5	0.0	6.460557332884324	0.002104731436268732
112-113	8.418925745074928E-5	8.418925745074928E-5	0.0	6.907223438289274	0.002104731436268732
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2430	0.0	43.101303	1
AGCCTCG	855	0.0	42.45148	2
TCAACGC	2925	0.0	36.209557	4
CAACGCA	2940	0.0	36.02482	5
GTACATG	6405	0.0	35.77055	1
GGTATCA	1565	0.0	35.363285	1
TACATGG	6675	0.0	33.784466	2
ATCAACG	3135	0.0	33.78404	3
AACGCAG	3200	0.0	33.280937	6
ACATGGG	6845	0.0	32.77155	3
TATCAAC	3250	0.0	32.40552	2
CATGGGG	5775	0.0	31.219053	4
ATGGGGG	3495	0.0	30.304136	5
TACGGTC	85	0.007209478	28.000784	5
ACGCAGA	3840	0.0	27.263556	7
CCTCGGT	770	0.0	27.046211	4
CGGTCTC	540	0.0	26.437386	7
GTATAGG	230	8.265488E-9	25.873558	1
TCGGTCT	465	0.0	25.589954	6
CGCAGAG	4175	0.0	25.360893	8
>>END_MODULE