FastQCFastQC Report
Mon 15 Aug 2016
SRR2921607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187800
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18180.1530560700454622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.1341134871190436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21500.051.3175541
GTATCAA33900.042.3984531
GTACATG63150.035.7930371
TCAACGC41050.034.7112964
CAACGCA41400.034.3801275
CATGGGG53800.033.577354
TACATGG66100.033.468162
ATCAACG42750.033.3365863
TATCAAC42750.033.1971052
ACATGGG67250.032.7185023
AACGCAG43900.032.564786
ATGGGGG33550.029.1112775
ACGCAGA51950.027.518657
CGCAGAG53700.026.7327868
GTATAGG2506.82121E-1026.24131
TATACCG1608.680476E-626.0548135
ATGGGGA19800.024.9644995
GCAGAGT59600.023.886539
GAGTACT24100.023.71420312-13
GCTACAC7450.023.2113883
Produced by FastQC (version 0.11.5)