##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921607_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1187800
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.689739013301903	33.0	31.0	34.0	30.0	34.0
2	31.853826401751135	33.0	31.0	34.0	30.0	34.0
3	31.926397541673683	34.0	31.0	34.0	30.0	34.0
4	35.33800303081327	37.0	35.0	37.0	33.0	37.0
5	35.2058519952854	37.0	35.0	37.0	33.0	37.0
6	35.23718892069372	37.0	35.0	37.0	33.0	37.0
7	35.11424987371611	37.0	35.0	37.0	33.0	37.0
8	35.150030308132685	37.0	35.0	37.0	33.0	37.0
9	36.852637649435934	39.0	38.0	39.0	33.0	39.0
10-11	36.80593534265027	39.0	38.0	39.0	33.0	39.0
12-13	36.747908317898634	39.0	38.0	39.0	33.0	39.0
14-15	38.16648383566257	41.0	39.0	41.0	33.0	41.0
16-17	38.17239981478363	41.0	39.0	41.0	33.0	41.0
18-19	38.18730804849301	41.0	39.0	41.0	33.5	41.0
20-21	38.12558974574844	41.0	39.0	41.0	33.0	41.0
22-23	38.13930038727058	41.0	39.0	41.0	34.0	41.0
24-25	38.10706263680754	41.0	39.0	41.0	33.5	41.0
26-27	38.036830274456975	41.0	39.0	41.0	33.0	41.0
28-29	37.964970954706175	41.0	38.5	41.0	33.0	41.0
30-31	37.84335830947971	41.0	38.0	41.0	33.0	41.0
32-33	37.722650698770835	41.0	38.0	41.0	33.0	41.0
34-35	37.594384576528036	40.5	38.0	41.0	32.0	41.0
36-37	37.520897878430716	40.0	38.0	41.0	32.0	41.0
38-39	37.4337582084526	40.0	38.0	41.0	32.0	41.0
40-41	37.30702475164169	40.0	38.0	41.0	32.0	41.0
42-43	37.143589829937696	40.0	37.5	41.0	31.0	41.0
44-45	36.9865751810069	40.0	37.0	41.0	31.0	41.0
46-47	36.7750858730426	40.0	37.0	41.0	30.5	41.0
48-49	36.57009134534434	40.0	36.5	41.0	30.0	41.0
50-51	36.31192372453275	40.0	36.0	41.0	30.0	41.0
52-53	36.18573665600269	40.0	36.0	41.0	29.0	41.0
54-55	36.051869843407985	40.0	35.0	41.0	29.0	41.0
56-57	35.85519868664758	39.0	35.0	41.0	29.0	41.0
58-59	35.65725458831453	39.0	35.0	41.0	29.0	41.0
60-61	35.39101069203569	38.5	35.0	41.0	28.0	41.0
62-63	35.034308806196336	37.5	35.0	40.5	28.0	41.0
64-65	34.74641774709547	37.0	35.0	40.0	28.0	41.0
66-67	34.43774162316888	36.5	35.0	39.5	28.0	41.0
68-69	34.06578801144974	36.0	35.0	39.0	27.5	41.0
70-71	33.69592818656339	35.5	34.0	39.0	27.0	41.0
72-73	33.374124431722514	35.0	34.0	37.5	26.0	40.0
74-75	33.05206263680754	35.0	34.0	37.0	26.0	39.0
76-77	32.73378430712241	35.0	34.0	36.5	26.0	39.0
78-79	32.47854563057754	35.0	34.0	36.0	26.0	38.0
80-81	32.232774456979286	35.0	34.0	36.0	26.0	37.0
82-83	32.01647836336083	35.0	34.0	35.0	25.5	37.0
84-85	31.808958157939045	35.0	34.0	35.0	25.0	36.0
86-87	31.688697171240946	35.0	33.5	35.0	25.0	36.0
88-89	31.59564025930291	35.0	33.0	35.0	25.0	36.0
90-91	31.495735393163834	35.0	33.0	35.0	24.5	35.5
92-93	31.417060953022393	35.0	33.0	35.0	24.0	35.0
94-95	31.35143795251726	35.0	33.0	35.0	24.0	35.0
96-97	31.287668799461187	35.0	33.0	35.0	24.0	35.0
98-99	31.212151035527867	35.0	33.0	35.0	23.0	35.0
100-101	31.143104478868494	35.0	33.0	35.0	23.0	35.0
102-103	30.44984172419599	34.0	31.5	35.0	20.0	35.0
104-105	30.967305943761573	35.0	33.0	35.0	20.0	35.0
106-107	31.067477689846775	35.0	33.0	35.0	23.0	35.0
108-109	31.056740191951505	35.0	33.0	35.0	21.5	35.0
110-111	30.98513512375821	35.0	33.0	35.0	20.0	35.0
112-113	30.94907391816804	35.0	33.0	35.0	19.5	35.0
114-115	30.829926755346015	35.0	33.0	35.0	19.0	35.0
116-117	31.015721501936355	35.0	33.0	35.0	20.0	35.0
118-119	31.00054891395858	35.0	33.0	35.0	19.0	35.0
120-121	30.925902087893583	35.0	33.0	35.0	18.0	35.0
122-123	30.794874978952684	35.0	33.0	35.0	6.0	35.0
124-125	30.154009513386093	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19176.0
3	5030.0
4	2651.0
5	2145.0
6	2287.0
7	2602.0
8	2871.0
9	2989.0
10	3368.0
11	3632.0
12	4008.0
13	5158.0
14	7024.0
15	6450.0
16	3770.0
17	3207.0
18	2828.0
19	2635.0
20	2556.0
21	2604.0
22	2848.0
23	3479.0
24	4759.0
25	6128.0
26	7441.0
27	8567.0
28	10369.0
29	12761.0
30	15002.0
31	18934.0
32	24554.0
33	33481.0
34	51371.0
35	92119.0
36	214637.0
37	440786.0
38	153323.0
39	250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90107932904252	17.934529518811416	11.273965323701871	26.890425828444197
2	16.29389716579557	19.409720351312032	37.18158979470877	27.11479268818363
3	19.143143234186688	23.186066076991292	28.237719517648575	29.43307117117345
4	13.49463811831135	14.730775944432404	33.73891426639154	38.03567167086471
5	14.760347712442517	37.26094348535999	32.58890489780074	15.389803904396762
6	35.42810010935665	33.578810085075766	16.011987106362398	14.981102699205184
7	30.92554155729944	31.405521879622484	18.697583756482008	18.971352806596066
8	28.64573024102018	34.6544310567743	17.877438944783933	18.822399757421593
9	28.18291379584783	15.171145859914988	17.865567775748097	38.780372568489085
10-11	27.288951377197485	24.93647154561265	25.71890454325853	22.055672533931332
12-13	27.072160615059854	22.12458297761041	26.599131607864805	24.204124799464932
14-15	24.503556166197594	23.39669259274547	23.83932851090858	28.260422730148356
16-17	24.647264772636817	26.758474763653396	24.483305697967424	24.110954765742367
18-19	24.641453481492274	25.650693973908652	26.4305118591864	23.277340685412675
20-21	25.26558096019254	24.649576291525577	26.37139986745163	23.713442880830254
22-23	25.108018454906716	24.208804809052335	26.280940594059405	24.402236141981547
24-25	24.605163510318135	24.663299202644353	26.261925499594398	24.46961178744311
26-27	24.35539240964591	24.92246944346805	26.37853265502428	24.343605491861766
28-29	24.400107936580103	25.16884034268601	26.333873437203348	24.09717828353054
30-31	24.922112478799566	24.85770609726374	26.255156193894358	23.965025230042336
32-33	24.58438053861083	24.805946021040935	26.219788917095023	24.38988452325322
34-35	24.56966918094648	24.95270465392941	26.38078415557496	24.096842009549153
36-37	24.65570444088018	25.00178914975394	26.267370539228686	24.075135870137196
38-39	24.729461853563585	25.066647920075535	26.24599933573082	23.957890890630058
40-41	24.70138104557362	24.873217179066977	26.25518886609621	24.170212909263196
42-43	24.540244546205393	24.865438968268602	26.14053787367891	24.453778611847095
44-45	24.503205040503445	24.884181156037293	26.221975628473775	24.390638174985487
46-47	24.52907313274187	24.99394857870531	25.979183110746273	24.497795177806545
48-49	24.475521237406596	25.234503687358163	25.83663818342623	24.45333689180901
50-51	24.5591776254862	25.38630891242486	25.71027462998198	24.344238832106956
52-53	24.704282245653264	25.2895718764628	25.608147245131285	24.397998632752646
54-55	24.483827594248876	25.000989279577148	25.800958632958753	24.714224493215227
56-57	24.545931749691338	25.233511493408557	25.5573483348691	24.66320842203101
58-59	24.462920408035263	25.27569068425003	25.79964715970296	24.461741748011747
60-61	24.770499697760055	25.18180700758883	25.883188892387427	24.164504402263685
62-63	24.62245009639751	25.167327557901647	25.892161072243407	24.318061273457428
64-65	24.47853173935006	24.89669978110793	25.89198518269069	24.73278329685132
66-67	24.461338991041963	25.277286657237152	25.72211557890483	24.53925877281606
68-69	24.468153174579786	25.178629308929573	25.754865107194426	24.59835240929622
70-71	24.32345512712578	25.093786832800134	25.95748442498737	24.625273615086716
72-73	24.515932948552102	25.408779191673165	25.538643909260557	24.536643950514176
74-75	24.346872142697176	25.392676548689703	25.645803058903166	24.614648249709955
76-77	24.404761654194527	25.029540390669823	25.65995989999836	24.90573805513729
78-79	24.27231914530886	25.119054384270044	25.79299080861847	24.81563566180262
80-81	24.411054049503285	25.323749789526858	25.54655665937026	24.718639501599597
82-83	24.426541924353927	25.272021341577645	25.560344809440878	24.741091924627558
84-85	24.41803097885295	25.369513494946027	25.42402704882562	24.7884284773754
86-87	24.24381208957737	25.510018521636642	25.51039737329517	24.735772015490824
88-89	24.137692979594412	25.241004809361943	25.512728497311176	25.10857371373246
90-91	24.2690104020869	25.12728465162838	25.67978107250395	24.92392387378077
92-93	24.28485435258461	25.334399730594377	25.59917494527698	24.78157097154403
94-95	24.226665033342133	25.121836328252765	25.622526071100975	25.028972567304127
96-97	24.096581817936897	25.10068148113225	25.634365945559807	25.168370755371043
98-99	24.12379440171343	25.11273017498013	25.47474642024866	25.288729003057774
100-101	23.97790031991918	25.25336757029803	25.55733288432396	25.21139922545883
102-103	23.961839943457854	25.068309835142934	25.73656966129856	25.233280560100656
104-105	24.07816972554302	25.13314531065836	25.55602795083347	25.23265701296515
106-107	24.120615495486998	24.97101762852219	25.640958720852407	25.267408155138405
108-109	24.0591511575679	24.977594577403632	25.65066231229013	25.312591952738345
110-111	23.908811132527948	25.30795416899415	25.57390836913837	25.209326329339532
112-113	23.857537886329855	25.297220525569823	25.5765192101509	25.26872237794942
114-115	23.83801986866476	25.34054554638828	25.49814783633608	25.32328674861088
116-117	23.7015970597162	25.127328247359664	25.596313784621938	25.574760908302192
118-119	23.776518740815234	25.036023433859377	25.629573439605448	25.55788438571994
120-121	23.62584118212135	25.187305885096606	25.645562288099562	25.541290644682483
122-123	23.58583175546375	25.27494500258044	25.49678687100253	25.64243637095328
124-125	23.49431200454433	25.18174784709077	25.49623525326708	25.827704895097824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	19.0
2	18.5
3	33.0
4	37.5
5	47.0
6	90.5
7	264.5
8	449.0
9	494.5
10	459.5
11	388.0
12	375.5
13	425.0
14	490.0
15	529.0
16	564.0
17	614.5
18	647.5
19	696.5
20	791.5
21	847.5
22	921.0
23	1081.0
24	1219.0
25	1424.5
26	1820.5
27	2401.5
28	3010.5
29	3815.5
30	4924.5
31	6115.5
32	7807.5
33	9629.0
34	11304.5
35	13064.5
36	15271.5
37	17651.0
38	19724.5
39	22182.0
40	24639.5
41	27003.0
42	29569.0
43	32884.0
44	36620.0
45	40209.5
46	43341.0
47	46174.5
48	50333.5
49	53317.0
50	54550.5
51	55410.0
52	55032.0
53	54682.0
54	54780.5
55	53045.0
56	49606.5
57	45506.0
58	41623.0
59	37552.0
60	31898.5
61	26827.5
62	22421.5
63	17186.5
64	12596.0
65	9473.5
66	7188.0
67	5619.0
68	4288.0
69	3216.0
70	2483.5
71	1925.0
72	1480.0
73	1147.0
74	845.0
75	620.0
76	445.0
77	256.0
78	139.5
79	76.0
80	45.5
81	25.5
82	17.5
83	9.5
84	7.5
85	5.5
86	4.0
87	3.5
88	1.5
89	1.5
90	1.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025509345007577035
2	0.002020542178817983
3	0.03089745748442499
4	0.16240107762249537
5	0.02247853173935006
6	0.07223438289274288
7	0.05623842397710052
8	0.046725037885165854
9	0.035443677386765446
10-11	0.0617528203401246
12-13	0.05585957231857215
14-15	0.059521805017679744
16-17	0.05165010944603468
18-19	0.03969523488802829
20-21	0.02601448055228153
22-23	0.0033675702980299716
24-25	0.005177639333221081
26-27	0.0035780434416568447
28-29	0.005514396363024078
30-31	0.002483583094797104
32-33	0.009681764606836169
34-35	0.004293652129988214
36-37	0.007156086883313689
38-39	0.0023993938373463547
40-41	0.003409664926755346
42-43	0.004125273615086715
44-45	0.005177639333221081
46-47	0.00526182859067183
48-49	0.002146826064994107
50-51	0.0016837851490149858
52-53	0.0010102710894089916
54-55	0.0056406802492002025
56-57	8.839872032328674E-4
58-59	8.839872032328674E-4
60-61	1.6837851490149856E-4
62-63	8.418925745074929E-4
64-65	0.0
66-67	0.0033675702980299716
68-69	4.2094628725374646E-4
70-71	0.0
72-73	0.002146826064994107
74-75	0.004546219902340462
76-77	0.001641690520289611
78-79	2.1047314362687323E-4
80-81	0.0
82-83	0.0060195319077285744
84-85	0.0018521636639164843
86-87	0.0
88-89	0.001052365718134366
90-91	7.997979457821182E-4
92-93	0.0
94-95	0.003241286411853848
96-97	0.001641690520289611
98-99	6.735140596059942E-4
100-101	0.0
102-103	0.0011365549755851154
104-105	0.0
106-107	7.577033170567436E-4
108-109	0.002483583094797104
110-111	1.2628388617612395E-4
112-113	2.9466240107762245E-4
114-115	0.0
116-117	0.001978447550092608
118-119	0.0026519616096986024
120-121	0.003115002525677724
122-123	0.001431217376662738
124-125	0.1065835999326486
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1187800.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.703093763074826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.87056139098875	51.51704954962655
2	10.628024969992143	13.5407614235942
3	3.4051879559438323	6.50763022915149
4	1.5694587045555672	3.9991750005430897
5	0.9039634536804387	2.8792634324098962
6	0.580878794151328	2.2202265773282335
7	0.39641949874091725	1.7677203948462636
8	0.28168649142379626	1.4355440779969333
9	0.22342463890713476	1.2809556649154106
>10	1.0942055018684078	11.959327932835311
>50	0.03638279387951569	1.548907994285935
>100	0.009275765291064773	0.916814109925054
>500	1.3251014426186812E-4	0.04592070542592962
>1k	3.975304327856044E-4	0.38070290711570326
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1818	0.1530560700454622	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1593	0.1341134871190436	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	1.6837851490149856E-4
14-15	0.0	0.0	0.0	0.0	2.5256777235224784E-4
16-17	0.0	0.0	0.0	0.0	3.367570298029971E-4
18-19	0.0	0.0	0.0	0.0	3.367570298029971E-4
20-21	0.0	0.0	0.0	4.209462872537464E-5	3.367570298029971E-4
22-23	0.0	0.0	0.0	1.6837851490149856E-4	4.209462872537464E-4
24-25	0.0	0.0	0.0	3.367570298029971E-4	5.051355447044957E-4
26-27	0.0	0.0	0.0	6.735140596059942E-4	5.051355447044957E-4
28-29	0.0	0.0	0.0	0.0025256777235224785	5.051355447044957E-4
30-31	0.0	0.0	0.0	0.010439467923892911	5.051355447044957E-4
32-33	0.0	0.0	0.0	0.025509345007577032	5.89324802155245E-4
34-35	0.0	0.0	0.0	0.043231183700959755	6.735140596059942E-4
36-37	0.0	0.0	0.0	0.0684037716787338	6.735140596059942E-4
38-39	0.0	0.0	0.0	0.1083936689678397	7.577033170567436E-4
40-41	0.0	0.0	0.0	0.15705505977437278	7.577033170567436E-4
42-43	0.0	0.0	0.0	0.20276982657012965	7.997979457821182E-4
44-45	0.0	0.0	0.0	0.2537885165852837	8.418925745074928E-4
46-47	0.0	0.0	0.0	0.31120559016669475	8.418925745074928E-4
48-49	0.0	0.0	0.0	0.37279003199191785	8.418925745074928E-4
50-51	0.0	0.0	0.0	0.4341219060447887	8.418925745074928E-4
52-53	0.0	0.0	0.0	0.5009260818319582	9.260818319582421E-4
54-55	0.0	4.209462872537464E-5	0.0	0.5730762754672504	0.0010102710894089914
56-57	0.0	8.418925745074928E-5	0.0	0.6588230341808385	0.0010102710894089914
58-59	0.0	8.418925745074928E-5	0.0	0.7445697928944266	0.0010102710894089914
60-61	0.0	8.418925745074928E-5	0.0	0.8333473648762417	0.0010102710894089914
62-63	0.0	8.418925745074928E-5	0.0	0.9338272436437111	0.0010102710894089914
64-65	0.0	8.418925745074928E-5	0.0	1.0339703653813772	0.0010102710894089914
66-67	0.0	8.418925745074928E-5	0.0	1.150572486950665	0.0010102710894089914
68-69	0.0	8.418925745074928E-5	0.0	1.2683532581242634	0.0010102710894089914
70-71	0.0	8.418925745074928E-5	0.0	1.395731604647247	0.0010102710894089914
72-73	0.0	8.418925745074928E-5	0.0	1.5162485266879946	0.0012628388617612393
74-75	0.0	8.418925745074928E-5	0.0	1.64661559185048	0.0013049334904866139
76-77	0.0	8.418925745074928E-5	0.0	1.803249705337599	0.0013470281192119885
78-79	0.0	8.418925745074928E-5	0.0	1.9772689004882977	0.0013470281192119885
80-81	0.0	8.418925745074928E-5	0.0	2.1520037043273277	0.001389122747937363
82-83	0.0	8.418925745074928E-5	0.0	2.3353258124263343	0.0015995958915642364
84-85	0.0	8.418925745074928E-5	0.0	2.5209210304765115	0.0015995958915642364
86-87	0.0	8.418925745074928E-5	0.0	2.735393163832295	0.001641690520289611
88-89	0.0	8.418925745074928E-5	0.0	2.9796261996969187	0.0017258797777403604
90-91	0.0	8.418925745074928E-5	0.0	3.2333305270247514	0.001767974406465735
92-93	0.0	8.418925745074928E-5	0.0	3.477605657518101	0.0018521636639164843
94-95	0.0	8.418925745074928E-5	0.0	3.730173429870349	0.0018521636639164843
96-97	0.0	8.418925745074928E-5	0.0	4.016585283717797	0.0018521636639164843
98-99	0.0	8.418925745074928E-5	0.0	4.3449233877757205	0.0018521636639164843
100-101	0.0	8.418925745074928E-5	0.0	4.692035696245159	0.0018521636639164843
102-103	0.0	8.418925745074928E-5	0.0	5.04432564404782	0.0018942582926418589
104-105	0.0	8.418925745074928E-5	0.0	5.405118706853006	0.0019363529213672335
106-107	0.0	8.418925745074928E-5	0.0	5.798072066004378	0.001978447550092608
108-109	0.0	8.418925745074928E-5	0.0	6.227353089745748	0.0020626368075433573
110-111	0.0	8.418925745074928E-5	0.0	6.686815962283212	0.0021889206937194816
112-113	0.0	8.418925745074928E-5	0.0	7.151919515069877	0.002273109951170231
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2150	0.0	51.317554	1
GTATCAA	3390	0.0	42.398453	1
GTACATG	6315	0.0	35.793037	1
TCAACGC	4105	0.0	34.711296	4
CAACGCA	4140	0.0	34.380127	5
CATGGGG	5380	0.0	33.57735	4
TACATGG	6610	0.0	33.46816	2
ATCAACG	4275	0.0	33.336586	3
TATCAAC	4275	0.0	33.197105	2
ACATGGG	6725	0.0	32.718502	3
AACGCAG	4390	0.0	32.56478	6
ATGGGGG	3355	0.0	29.111277	5
ACGCAGA	5195	0.0	27.51865	7
CGCAGAG	5370	0.0	26.732786	8
GTATAGG	250	6.82121E-10	26.2413	1
TATACCG	160	8.680476E-6	26.054813	5
ATGGGGA	1980	0.0	24.964499	5
GCAGAGT	5960	0.0	23.88653	9
GAGTACT	2410	0.0	23.714203	12-13
GCTACAC	745	0.0	23.211388	3
>>END_MODULE