FastQCFastQC Report
Mon 15 Aug 2016
SRR2921606_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921606_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013515
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG701.8189894E-1276.553171
CGTAGCG1900.059.5266533
GCGTAGC3050.052.6957242
GGCGTAG3000.035.7248151
GTACATG63700.034.77141
TACATGG63850.034.028552
CATGGGG53050.033.3259474
ATGGGGG31150.033.2475555
ACATGGG65850.032.543043
GTATCAA24550.030.0738411
GTAGCGT1602.59226E-729.7633254
TATAGGG1602.59226E-729.7633252
GTACCGT2154.0272425E-927.678616
GGTATCA18950.027.3356081
TACCGTC1755.7114266E-727.1933787
TCAACGC28850.025.172454
TAGCGGA1200.001285139724.800325
CAACGCA29200.024.6644275
ACCGTGC3202.0008883E-1124.1659898
TGGGGGG25150.024.1348676
Produced by FastQC (version 0.11.5)