##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921606_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1013515
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.82462519054972	31.0	30.0	31.0	23.0	34.0
2	28.99568925965575	31.0	30.0	33.0	23.0	34.0
3	29.401188931589566	31.0	30.0	34.0	25.0	34.0
4	32.810582971144974	35.0	35.0	37.0	26.0	37.0
5	33.17993517609508	37.0	35.0	37.0	27.0	37.0
6	33.068757739155316	37.0	35.0	37.0	26.0	37.0
7	32.8764251145765	37.0	35.0	37.0	25.0	37.0
8	32.98401503677795	37.0	35.0	37.0	26.0	37.0
9	34.58768148473382	39.0	35.0	39.0	26.0	39.0
10-11	34.680642615057494	39.0	37.0	39.0	27.0	39.0
12-13	34.62673172079347	39.0	36.0	39.0	25.5	39.0
14-15	35.831616700295505	40.0	37.0	41.0	25.5	41.0
16-17	35.781885319901534	40.0	37.0	41.0	25.0	41.0
18-19	35.68901545611067	40.0	37.0	41.0	25.0	41.0
20-21	35.77921047049131	40.0	37.0	41.0	25.0	41.0
22-23	35.70396787418045	40.0	37.0	41.0	25.0	41.0
24-25	35.69819242931777	40.0	37.0	41.0	25.0	41.0
26-27	35.669288071710824	40.0	37.0	41.0	25.0	41.0
28-29	35.62773515932177	40.0	36.5	41.0	25.0	41.0
30-31	35.52647222784073	40.0	36.0	41.0	24.0	41.0
32-33	35.43216380615975	40.0	36.0	41.0	24.0	41.0
34-35	35.30079426550175	40.0	36.0	41.0	23.5	41.0
36-37	35.190734226923134	40.0	35.5	41.0	23.0	41.0
38-39	35.05903810007745	40.0	35.0	41.0	22.0	41.0
40-41	34.94750349032822	40.0	35.0	41.0	20.5	41.0
42-43	34.671770028070625	40.0	35.0	41.0	18.5	41.0
44-45	34.25987775217929	39.0	34.5	41.0	15.5	41.0
46-47	33.989630148542446	39.0	34.0	40.0	14.5	41.0
48-49	33.783663290627175	38.0	34.0	40.0	11.0	41.0
50-51	33.569631431207235	38.0	33.0	40.0	9.0	41.0
52-53	33.35421922714514	38.0	33.0	40.0	8.0	41.0
54-55	33.11171960947791	38.0	33.0	40.0	7.0	41.0
56-57	32.85670858349408	37.5	33.0	40.0	4.0	41.0
58-59	32.87064473638772	37.0	33.0	40.0	2.0	41.0
60-61	32.663768173140014	37.0	33.0	40.0	2.0	41.0
62-63	32.352525616295765	36.0	32.0	39.5	2.0	41.0
64-65	32.0626626147615	36.0	32.0	39.0	2.0	41.0
66-67	31.74508221387942	35.5	32.0	39.0	2.0	40.5
68-69	31.417251841363964	35.0	31.5	38.5	2.0	40.0
70-71	31.05933212631288	35.0	31.0	37.5	2.0	40.0
72-73	30.71155335638841	35.0	31.0	37.0	2.0	39.0
74-75	30.34973976704834	35.0	31.0	36.5	2.0	39.0
76-77	30.06165818956799	35.0	31.0	36.0	2.0	38.5
78-79	29.792850130486478	35.0	30.0	35.5	2.0	37.0
80-81	29.538654089974003	34.5	30.0	35.0	2.0	37.0
82-83	29.320823076126153	34.0	30.0	35.0	2.0	36.5
84-85	29.11566084369743	34.0	30.0	35.0	2.0	36.0
86-87	28.941614085632676	34.0	30.0	35.0	2.0	36.0
88-89	28.78944810880944	34.0	29.0	35.0	2.0	35.0
90-91	28.60717946947011	34.0	29.0	35.0	2.0	35.0
92-93	28.527630079475884	34.0	29.0	35.0	2.0	35.0
94-95	28.405603271781867	34.0	29.0	35.0	2.0	35.0
96-97	28.32092568930899	34.0	29.0	35.0	2.0	35.0
98-99	28.23259103219982	34.0	29.0	35.0	2.0	35.0
100-101	28.115581910479868	34.0	29.0	35.0	2.0	35.0
102-103	27.225196963044453	32.5	26.0	34.0	2.0	35.0
104-105	28.178813337740436	34.0	29.0	35.0	2.0	35.0
106-107	28.472421227115532	34.0	29.5	35.0	2.0	35.0
108-109	28.48604164713892	34.0	30.0	35.0	2.0	35.0
110-111	28.435226908333867	34.0	30.0	35.0	2.0	35.0
112-113	28.356990769746872	34.0	29.0	35.0	2.0	35.0
114-115	28.23680113269167	34.0	29.0	35.0	2.0	35.0
116-117	28.118664252625763	34.0	29.0	35.0	2.0	35.0
118-119	27.829123397285684	34.0	29.0	35.0	2.0	35.0
120-121	27.697443550416125	34.0	29.0	35.0	2.0	35.0
122-123	27.42651909443866	34.0	29.0	35.0	2.0	35.0
124-125	26.611863662599962	33.5	26.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	54134.0
3	7996.0
4	4327.0
5	3635.0
6	3739.0
7	4230.0
8	4261.0
9	4445.0
10	4611.0
11	4887.0
12	4905.0
13	4947.0
14	4706.0
15	4476.0
16	3862.0
17	3522.0
18	3461.0
19	3125.0
20	3010.0
21	3181.0
22	3654.0
23	4920.0
24	6876.0
25	8599.0
26	9351.0
27	10775.0
28	12958.0
29	15603.0
30	18977.0
31	23985.0
32	31865.0
33	44157.0
34	68503.0
35	108502.0
36	200553.0
37	254883.0
38	53868.0
39	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.912951482027566	17.29818390682086	11.374712071956845	25.414152539194728
2	17.429641916554097	19.310297871668592	37.65742439238107	25.602635819396248
3	20.562443570628606	23.208888581902144	27.646469679851197	28.58219816761805
4	14.44654185484977	14.552519737640601	33.50361499044324	37.49732341706638
5	14.725656660551618	37.52386892150468	32.696302451184046	15.054171966759657
6	34.749901003200485	33.705028049916116	16.56223862129305	14.982832325590351
7	31.414443158888172	31.412468755059407	18.804616945913182	18.368471140139235
8	28.400643205524695	34.349405903552736	18.429871743870745	18.82007914705183
9	28.650845423619753	15.873614276028617	17.430638550685448	38.04490174966618
10-11	27.192097073913484	25.31705234479304	25.80296483118921	21.687885750104265
12-13	26.57943556344977	22.732336688375764	26.20367081113085	24.484556937043617
14-15	24.48840116079681	23.938547444682783	23.272189679053795	28.300861715466613
16-17	25.102669252520464	27.05567974891557	23.847839282277526	23.993811716286437
18-19	24.98450334707962	26.349097147233998	25.28940246090797	23.376997044778417
20-21	25.550764050188924	25.23623207071492	25.16852923484446	24.044474644251697
22-23	25.630272628350486	24.781667846929384	24.93923908832724	24.64882043639289
24-25	25.153113568297456	25.163871366343983	24.918415919172418	24.76459914618614
26-27	24.75102150402309	25.409551673371407	25.133514857275692	24.705911965329815
28-29	24.9316701888558	25.89697374694132	24.912965242359846	24.258390821843033
30-31	25.571418420398157	25.343097778672576	25.06493839734404	24.02054540358523
32-33	24.927078671146642	25.30630412351548	25.201246089931452	24.565371115406425
34-35	24.87929464543496	25.74336879353869	25.13987903554164	24.23745752548471
36-37	25.071194006875675	25.81441404816129	24.935811917570856	24.178580027392176
38-39	25.11469598778681	25.75471390888071	25.09821568668086	24.032374416651617
40-41	24.867044902697007	25.521999281430507	25.23239998262799	24.3785558332445
42-43	24.89822926319467	25.40330817807436	25.073694349293945	24.624768209437022
44-45	24.682627751920467	25.508937836041568	25.326574304011256	24.48186010802671
46-47	24.589476364328963	25.398409863916203	25.283390711327584	24.728723060427242
48-49	24.54904108777877	25.64580079594772	25.293570489238913	24.51158762703459
50-51	24.546681023124624	25.636048000047374	25.40264041711366	24.414630559714347
52-53	24.587503367772612	25.596502845706176	25.308330068362082	24.50766371815913
54-55	24.48477390054012	25.210477127543562	25.445404241418817	24.859344730497497
56-57	24.56602974766836	25.43646213643781	25.07659459611493	24.9209135197789
58-59	24.530547356766245	25.742800443277368	25.2379458060849	24.488706393871485
60-61	24.871806005676458	25.45341594066167	25.38714783659065	24.287630217071214
62-63	24.62087083012191	25.556123551651076	25.43370284878551	24.389302769441503
64-65	24.52902247862961	25.26857314288308	25.339824221024397	24.86258015746291
66-67	24.544128031518365	25.571662683610814	25.103318478500626	24.78089080637019
68-69	24.338021775226178	25.550585582362707	25.396387572380547	24.715005070030568
70-71	24.292329795167387	25.404613878948233	25.61066552955768	24.692390796326706
72-73	24.402505047793674	25.779916195769527	25.239757863801348	24.57782089263545
74-75	24.392016435241832	25.61262127108676	25.291583451085025	24.70377884258638
76-77	24.279208380001283	25.23794486685449	25.43441899019603	25.0484277629482
78-79	24.31474876588656	25.254767201103746	25.595838456968373	24.83464557604132
80-81	24.382372184224053	25.590638208008464	25.363274199612572	24.663715408154907
82-83	24.393921824538204	25.654163757435498	25.34734369718666	24.604570720839636
84-85	24.507302151174265	25.673228734951646	25.130945332543913	24.688523781330176
86-87	24.204325438099616	25.78294455836756	25.32550322986834	24.687226773664484
88-89	24.1170198225528	25.39085802990283	25.40008516597249	25.09203698157188
90-91	24.517700673921347	25.25729703164736	25.43295671765074	24.792045576780552
92-93	24.31003800409199	25.57853496519539	25.52406720256235	24.587359828150262
94-95	24.289572569281354	25.34680147305517	25.483814944484134	24.879811013179346
96-97	24.249500802022546	25.338731792904014	25.48107784330685	24.93068956176659
98-99	24.16569556040807	25.37685665484894	25.348833537750792	25.1086142469922
100-101	24.074434971036236	25.550770335936228	25.441980459265963	24.93281423376157
102-103	23.753824689362702	25.461602141530033	25.72707332451225	25.057499844595014
104-105	24.001503738625757	25.490145775816647	25.61580267476028	24.892547810797318
106-107	23.981799775827227	25.410654521343773	25.73147219941905	24.876073503409952
108-109	23.9056682531052	25.45158237622375	25.70158706350326	24.94116230716779
110-111	23.64523027816902	25.806300428464656	25.614934348953263	24.933534944413065
112-113	23.602444024006097	25.957863526370446	25.600041440859204	24.83965100876425
114-115	23.66222352915636	26.001531481214684	25.591672768250973	24.74457222137798
116-117	23.497743865316703	25.797244881144678	25.660090837419176	25.04492041611945
118-119	23.633530232420078	25.604697873687755	25.7082430466918	25.053528847200372
120-121	23.554688155216425	25.789811751542423	25.77491290092462	24.880587192316533
122-123	23.401857908939768	25.895580189540258	25.720642720065516	24.981919181454458
124-125	23.325380458693303	25.76498785293665	25.748607009714185	25.161024678655856
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	324.0
1	242.5
2	135.5
3	115.0
4	118.5
5	109.5
6	105.0
7	117.5
8	127.0
9	133.5
10	160.0
11	165.0
12	153.5
13	176.0
14	203.5
15	233.5
16	276.0
17	305.0
18	326.0
19	354.5
20	418.5
21	483.5
22	559.5
23	703.0
24	891.5
25	1134.5
26	1434.0
27	1857.0
28	2430.5
29	3106.5
30	4017.0
31	5073.0
32	6319.0
33	7653.0
34	9059.0
35	10610.5
36	12467.5
37	14488.5
38	16526.5
39	18814.0
40	21057.5
41	23043.0
42	25298.0
43	28610.5
44	32074.5
45	35385.5
46	37955.5
47	40176.5
48	43424.5
49	46182.5
50	48263.5
51	48959.5
52	48191.5
53	47237.0
54	46301.0
55	44678.5
56	41447.5
57	37793.5
58	34650.0
59	31286.5
60	26993.0
61	22648.5
62	18688.5
63	14562.5
64	10863.0
65	8294.5
66	6459.0
67	5050.0
68	3877.5
69	2941.0
70	2280.0
71	1780.0
72	1368.5
73	1032.5
74	800.0
75	606.5
76	416.5
77	259.0
78	189.0
79	131.0
80	83.5
81	72.0
82	62.5
83	49.5
84	37.5
85	32.5
86	27.5
87	18.5
88	13.5
89	16.0
90	14.5
91	10.5
92	8.5
93	7.0
94	8.0
95	7.0
96	4.5
97	4.0
98	4.5
99	5.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02377863179134004
2	0.009077320019930637
3	0.007893321756461425
4	0.009471986107753709
5	0.01657597568856899
6	0.08445854279413724
7	0.054365253597628053
8	0.04578126618747626
9	0.0232852991815612
10-11	0.04661993162410028
12-13	0.011346650024913297
14-15	0.024222631140140994
16-17	0.030586621806288017
18-19	0.03877594312861674
20-21	0.025998628535344813
22-23	0.03181995333073512
24-25	0.029254623759885155
26-27	0.0419826050921792
28-29	0.04084794008968787
30-31	0.024370630923074645
32-33	0.02619596157925635
34-35	0.04020660769697538
36-37	0.0085839874101518
38-39	0.018154640039861274
40-41	0.038578610084705214
42-43	0.0205226365667997
44-45	0.015243977642166125
46-47	0.020275970261910282
48-49	0.025406629403610204
50-51	0.026245294840234232
52-53	0.023087966137649664
54-55	0.02190396787418045
56-57	0.022249300701025636
58-59	0.01519464438118824
60-61	0.020423970044843934
62-63	0.020325303522888166
64-65	0.019437304825286255
66-67	0.026097295057300584
68-69	0.020473303305821818
70-71	0.04139060596044459
72-73	0.01983197091310933
74-75	0.008189321322328727
76-77	0.014799978293365169
78-79	0.02338396570351697
80-81	0.01598397655683438
82-83	0.021459968525379496
84-85	0.011346650024913297
86-87	0.015885310034878614
88-89	0.019683971130175677
90-91	0.018943972215507417
92-93	0.007054656319837397
94-95	0.010951983937090225
96-97	0.013763979812829607
98-99	0.006511990449080674
100-101	0.00922531980286429
102-103	0.003354661746496105
104-105	0.00404532740018648
106-107	0.0010853317415134456
108-109	0.013221313942072885
110-111	0.0012333315244470976
112-113	0.0022199967440047754
114-115	0.011543983068824832
116-117	0.005525325229522997
118-119	0.2807062549641594
120-121	0.0013813313073807493
122-123	9.86665219557678E-4
124-125	0.3145982052559656
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1013515.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.41887431303377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.99666005726306	59.85369121600065
2	8.659433546957047	12.19575125130473
3	2.6449135805241544	5.587555109872996
4	1.17210628877373	3.3015362172269502
5	0.7041592364403979	2.4793050383629107
6	0.4236066011521427	1.7897940002822497
7	0.2771100027941532	1.365964212035214
8	0.20442457401179445	1.151627870706562
9	0.1616925170365262	1.0247584531097889
>10	0.7164119975630456	8.501895355188758
>50	0.028287627779668093	1.335646266588157
>100	0.010914120702462804	1.2903923021211083
>500	2.798490018348079E-4	0.12208270719993226
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.86665219557678E-5
7	0.0	0.0	0.0	0.0	9.86665219557678E-5
8	0.0	0.0	0.0	0.0	9.86665219557678E-5
9	0.0	0.0	0.0	0.0	9.86665219557678E-5
10-11	0.0	0.0	0.0	0.0	9.86665219557678E-5
12-13	0.0	0.0	0.0	0.0	1.973330439115356E-4
14-15	0.0	0.0	0.0	0.0	3.453328268451873E-4
16-17	0.0	0.0	0.0	4.93332609778839E-5	5.426658707567229E-4
18-19	0.0	0.0	0.0	9.86665219557678E-5	6.906656536903745E-4
20-21	0.0	0.0	0.0	9.86665219557678E-5	6.906656536903745E-4
22-23	0.0	0.0	0.0	1.479997829336517E-4	7.399989146682584E-4
24-25	0.0	0.0	0.0	4.933326097788389E-4	0.0010853317415134458
26-27	0.0	0.0	0.0	0.0010853317415134458	0.0010853317415134458
28-29	0.0	0.0	0.0	0.004489326748987435	0.0010853317415134458
30-31	0.0	0.0	0.0	0.017118641559325715	0.0010853317415134458
32-33	0.0	0.0	0.0	0.039219942477417696	0.0010853317415134458
34-35	0.0	0.0	0.0	0.06625456949329808	0.0010853317415134458
36-37	0.0	0.0	0.0	0.1013798513095514	0.0011839982634692136
38-39	0.0	0.0	0.0	0.16092509730985727	0.0011839982634692136
40-41	0.0	0.0	0.0	0.23660231964993117	0.0011839982634692136
42-43	0.0	0.0	0.0	0.30191955718464947	0.0011839982634692136
44-45	0.0	0.0	0.0	0.3774981130027676	0.0012333315244470976
46-47	0.0	0.0	0.0	0.45736866252596164	0.001381331307380749
48-49	0.0	0.0	0.0	0.5422712046688998	0.001381331307380749
50-51	0.0	0.0	0.0	0.6290977439899754	0.001381331307380749
52-53	0.0	0.0	0.0	0.7222882739771981	0.001381331307380749
54-55	0.0	0.0	0.0	0.8264307879015111	0.001479997829336517
56-57	0.0	0.0	0.0	0.9350626285748114	0.001479997829336517
58-59	0.0	0.0	0.0	1.055929117970627	0.001479997829336517
60-61	0.0	0.0	0.0	1.1739342782297253	0.001479997829336517
62-63	0.0	0.0	0.0	1.3042234204723167	0.001479997829336517
64-65	0.0	0.0	0.0	1.4291352372683188	0.001479997829336517
66-67	0.0	0.0	0.0	1.5740763580213417	0.001479997829336517
68-69	0.0	0.0	0.0	1.7267628007478923	0.001529331090314401
70-71	0.0	0.0	0.0	1.8880332308845946	0.0015786643512922848
72-73	0.0	0.0	0.0	2.0371676788207376	0.0016773308732480525
74-75	0.0	0.0	0.0	2.197648776781794	0.0016773308732480525
76-77	0.0	0.0	0.0	2.3930578235151923	0.0016773308732480525
78-79	0.0	0.0	0.0	2.603464181585867	0.0016773308732480525
80-81	0.0	0.0	0.0	2.8054345520293236	0.001825330656181704
82-83	0.0	0.0	0.0	3.0208235694587646	0.0021706634830268917
84-85	0.0	0.0	0.0	3.2427739105982645	0.0021706634830268917
86-87	0.0	0.0	0.0	3.4853455548265195	0.0022199967440047754
88-89	0.0	0.0	0.0	3.767975806968816	0.002269330004982659
90-91	0.0	0.0	0.0	4.056723383472371	0.002269330004982659
92-93	0.0	0.0	0.0	4.321938994489475	0.002367996526938427
94-95	0.0	0.0	0.0	4.601263918146254	0.002367996526938427
96-97	0.0	0.0	0.0	4.918180786668179	0.002466663048894195
98-99	0.0	0.0	0.0	5.2935575694488985	0.0025653295708499627
100-101	0.0	0.0	0.0	5.684572995959606	0.0025653295708499627
102-103	0.0	0.0	0.0	6.078301751824097	0.0028119958757393823
104-105	0.0	0.0	0.0	6.477013167047355	0.002861329136717266
106-107	0.0	0.0	0.0	6.913267193874782	0.00291066239769515
108-109	0.0	0.0	0.0	7.386373166652689	0.002959995658673034
110-111	0.0	0.0	0.0	7.89342042298338	0.0030586621806288016
112-113	0.0	0.0	0.0	8.389071696028179	0.0030586621806288016
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAG	70	1.8189894E-12	76.55317	1
CGTAGCG	190	0.0	59.526653	3
GCGTAGC	305	0.0	52.695724	2
GGCGTAG	300	0.0	35.724815	1
GTACATG	6370	0.0	34.7714	1
TACATGG	6385	0.0	34.02855	2
CATGGGG	5305	0.0	33.325947	4
ATGGGGG	3115	0.0	33.247555	5
ACATGGG	6585	0.0	32.54304	3
GTATCAA	2455	0.0	30.073841	1
GTAGCGT	160	2.59226E-7	29.763325	4
TATAGGG	160	2.59226E-7	29.763325	2
GTACCGT	215	4.0272425E-9	27.67861	6
GGTATCA	1895	0.0	27.335608	1
TACCGTC	175	5.7114266E-7	27.193378	7
TCAACGC	2885	0.0	25.17245	4
TAGCGGA	120	0.0012851397	24.80032	5
CAACGCA	2920	0.0	24.664427	5
ACCGTGC	320	2.0008883E-11	24.165989	8
TGGGGGG	2515	0.0	24.134867	6
>>END_MODULE