FastQCFastQC Report
Mon 15 Aug 2016
SRR2921606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013515
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.16566109036373414No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15410.1520451103338382No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33850.042.6361851
GGTATCA22850.037.5835651
TCAACGC42850.033.2553334
TATCAAC43950.032.8348582
CAACGCA43750.032.4109045
GTACATG63950.031.9871251
ATCAACG44700.031.8837263
AACGCAG45350.031.4003376
CATGGGG51550.030.650074
ACATGGG64550.030.5780943
TACATGG65800.030.3597072
ATGGGGG28950.029.6350845
ACGCAGA51750.027.6307777
CGCAGAG53950.026.5027268
TGGGGGG19750.025.9444856
GCAGAGT58900.024.174279
GGGTTAG2853.1141099E-923.01811
GTAAGTA5600.022.3619652
GAGTACT24850.022.27620712-13
AGAGTAC54600.022.15002310-11
Produced by FastQC (version 0.11.5)