##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921606_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1013515 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87084650942512 33.0 31.0 34.0 30.0 34.0 2 32.03036166213623 33.0 31.0 34.0 30.0 34.0 3 32.101830757314886 34.0 31.0 34.0 30.0 34.0 4 35.51691489519149 37.0 35.0 37.0 35.0 37.0 5 35.401848023956234 37.0 35.0 37.0 33.0 37.0 6 35.44383457570929 37.0 36.0 37.0 33.0 37.0 7 35.33041444872548 37.0 36.0 37.0 33.0 37.0 8 35.365953143268726 37.0 36.0 37.0 33.0 37.0 9 37.09069328031652 39.0 38.0 39.0 34.0 39.0 10-11 37.04920400783412 39.0 38.0 39.0 34.0 39.0 12-13 36.99175394542755 39.0 38.0 39.0 34.0 39.0 14-15 38.427545226267 41.0 39.0 41.0 34.0 41.0 16-17 38.430943301283165 41.0 39.0 41.0 34.0 41.0 18-19 38.43922290247308 41.0 39.0 41.0 34.0 41.0 20-21 38.38135054735253 41.0 39.0 41.0 34.0 41.0 22-23 38.39632911205064 41.0 39.0 41.0 34.0 41.0 24-25 38.3659521566035 41.0 39.0 41.0 34.0 41.0 26-27 38.295210233691655 41.0 39.0 41.0 34.0 41.0 28-29 38.22029323690325 41.0 39.0 41.0 34.0 41.0 30-31 38.103695061247244 41.0 38.5 41.0 34.0 41.0 32-33 37.983730877194716 41.0 38.0 41.0 33.0 41.0 34-35 37.860933483964224 41.0 38.0 41.0 33.0 41.0 36-37 37.79212049155662 41.0 38.0 41.0 33.0 41.0 38-39 37.705604751779696 40.0 38.0 41.0 33.0 41.0 40-41 37.583818690399255 40.0 38.0 41.0 33.0 41.0 42-43 37.42142099524921 40.0 38.0 41.0 32.5 41.0 44-45 37.26575926355308 40.0 37.5 41.0 32.0 41.0 46-47 37.05330804181487 40.0 37.0 41.0 31.5 41.0 48-49 36.85052811255876 40.0 37.0 41.0 31.0 41.0 50-51 36.599477560766246 40.0 36.0 41.0 30.0 41.0 52-53 36.478330858448075 40.0 36.0 41.0 30.0 41.0 54-55 36.35289660241832 40.0 35.5 41.0 30.0 41.0 56-57 36.15266325609389 39.0 35.0 41.0 30.0 41.0 58-59 35.963153974040836 39.0 35.0 41.0 30.0 41.0 60-61 35.70556775183397 39.0 35.0 41.0 30.0 41.0 62-63 35.3429317770334 38.0 35.0 41.0 29.0 41.0 64-65 35.05483391957692 37.0 35.0 40.0 29.0 41.0 66-67 34.7502419796451 37.0 35.0 39.5 29.0 41.0 68-69 34.372175054143256 36.0 35.0 39.0 28.5 41.0 70-71 33.990123481152224 36.0 35.0 39.0 28.0 41.0 72-73 33.66390334627509 35.0 34.5 37.5 28.0 40.0 74-75 33.34139257929088 35.0 34.0 37.0 28.0 39.0 76-77 33.0149632713872 35.0 34.0 36.5 27.0 39.0 78-79 32.75039293942369 35.0 34.0 36.0 27.0 38.0 80-81 32.50300636892399 35.0 34.0 36.0 26.5 37.0 82-83 32.28575008756654 35.0 34.0 35.0 26.0 37.0 84-85 32.08314282472386 35.0 34.0 35.0 26.0 36.0 86-87 31.95915797990163 35.0 34.0 35.0 26.0 36.0 88-89 31.866777008727055 35.0 34.0 35.0 26.0 36.0 90-91 31.765007917988388 35.0 34.0 35.0 25.0 36.0 92-93 31.684894648821185 35.0 34.0 35.0 25.0 35.0 94-95 31.620309023546767 35.0 34.0 35.0 25.0 35.0 96-97 31.558719900544148 35.0 33.0 35.0 25.0 35.0 98-99 31.48123954751533 35.0 33.0 35.0 24.5 35.0 100-101 31.41461941855819 35.0 33.0 35.0 24.0 35.0 102-103 30.733510604184445 34.0 31.5 35.0 23.5 35.0 104-105 31.24037878077779 35.0 33.0 35.0 24.0 35.0 106-107 31.34142267258008 35.0 33.0 35.0 24.0 35.0 108-109 31.32484719022412 35.0 33.0 35.0 24.0 35.0 110-111 31.258901940277156 35.0 33.0 35.0 24.0 35.0 112-113 31.222380527175225 35.0 33.0 35.0 23.5 35.0 114-115 31.091284786115647 35.0 33.0 35.0 23.0 35.0 116-117 31.270984149223246 35.0 33.5 35.0 24.0 35.0 118-119 31.25360848137423 35.0 34.0 35.0 23.5 35.0 120-121 31.17075326956187 35.0 34.0 35.0 23.0 35.0 122-123 31.03595605393112 35.0 34.0 35.0 19.0 35.0 124-125 30.391939438488826 34.5 32.0 35.0 8.5 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13009.0 3 3701.0 4 2000.0 5 1620.0 6 1718.0 7 1966.0 8 2276.0 9 2377.0 10 2501.0 11 2809.0 12 3247.0 13 4231.0 14 5901.0 15 5577.0 16 3143.0 17 2671.0 18 2367.0 19 2078.0 20 2083.0 21 2119.0 22 2319.0 23 2837.0 24 3794.0 25 4814.0 26 5762.0 27 6987.0 28 8376.0 29 9977.0 30 12496.0 31 15875.0 32 20466.0 33 27742.0 34 43656.0 35 77494.0 36 180496.0 37 383553.0 38 139229.0 39 248.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.789286739981975 17.31351326278141 10.866233275335086 27.030966721901528 2 16.47742072983021 19.464704350091168 36.91578457142406 27.142090348654555 3 19.505767818651254 23.15812745260598 28.223523698983822 29.112581029758942 4 13.600030044394545 14.553050099027907 33.61355002194031 38.23336983463723 5 14.605225760099794 37.38206709247519 32.43974072524297 15.572966422182045 6 35.29311292970759 33.0715028300797 16.308920502059003 15.3264637381537 7 30.889852342792164 31.6493620079327 18.680936840463485 18.779848808811657 8 29.05986111865126 34.18119722237303 18.097907896100562 18.66103376287515 9 28.79735966300962 14.944473176181697 17.75604912732283 38.50211803348586 10-11 27.553228562712807 24.763239392542324 25.685157012783545 21.998375031961327 12-13 27.16042618681656 22.07401308807749 26.390916728118835 24.374643996987118 14-15 24.56849830301666 23.228977249169276 23.89370309603208 28.308821351781983 16-17 24.742398809138948 26.58006995716395 24.528045794451106 24.149485439246 18-19 24.6568825576271 25.573507400262642 26.54545346872969 23.224156573380572 20-21 25.27702871071921 24.502034200222543 26.427480577026984 23.793456512031263 22-23 25.03983778743642 24.163603802720317 26.275376547260198 24.521181862583067 24-25 24.523827261052727 24.533546330607226 26.292944596863354 24.649681811476693 26-27 24.319452187254868 24.765536737100206 26.435813060874114 24.479198014770816 28-29 24.462024223587164 25.326179728163 26.222896470065866 23.98889957818397 30-31 25.02252107805169 24.792031534442696 26.30308980310707 23.88235758439854 32-33 24.58693679759274 24.56976745907111 26.4221812065802 24.421114536755955 34-35 24.539900303509903 25.033547908386584 26.397221049141766 24.02933073896175 36-37 24.643497806519857 25.035077921334675 26.37667273828812 23.944751533857346 38-39 24.690773323229692 25.053182252322653 26.39364141545699 23.86240300899066 40-41 24.610205982531543 24.778536217641324 26.503033101657252 24.108224698169877 42-43 24.5100060977896 24.729644927092934 26.28660400480719 24.47374497031028 44-45 24.380988090422015 24.921704638519145 26.275469426820724 24.421837844238112 46-47 24.398047307647715 24.89559410837492 26.049942154325844 24.65641642965152 48-49 24.300467982701512 25.248224221237514 26.001851013173177 24.4494567828878 50-51 24.505130735076467 25.244893931919094 25.942673902318695 24.307301430685744 52-53 24.5267045911443 25.29300531669537 25.7344928073898 24.44579728477053 54-55 24.315457263841942 24.89352160427285 26.01190860890102 24.779112522984185 56-57 24.46169587383215 25.121928116924696 25.64644348781015 24.769932521433006 58-59 24.339275072323062 25.24592800451501 25.937733101268073 24.477063821893857 60-61 24.749337453651354 25.096643953610904 26.012960847112126 24.141057745625616 62-63 24.502196836121847 25.072446751514306 26.104214787721396 24.321141624642454 64-65 24.297617696827377 24.889172829213184 26.00430186035727 24.808907613602166 66-67 24.311507938465667 25.271958187754507 25.735804152610353 24.680729721169474 68-69 24.227117416584527 25.162134402093315 25.919797614431417 24.69095056689074 70-71 24.10518053762428 25.066143602286893 26.116745246367962 24.71193061372087 72-73 24.25112950952794 25.31181764452246 25.80121421657574 24.635838629373858 74-75 24.248806452679187 25.30853142740711 25.690782316801524 24.75187980311218 76-77 24.079548336107557 24.901899068275483 25.8220793944136 25.196473201203357 78-79 24.010150831809753 24.989121994488478 26.022606518120632 24.978120655581133 80-81 24.152429909769467 25.354977479366365 25.63287173845478 24.859720872409387 82-83 24.187799190006963 25.386261298485245 25.65366063274971 24.772278878758083 84-85 24.228760194889386 25.370202999907253 25.488900816775168 24.912135988428197 86-87 23.91661692229518 25.553889187629192 25.56656783570051 24.96292605437512 88-89 23.84370043487806 25.153267998845585 25.760321262157714 25.24271030411864 90-91 24.080038874897326 25.00050566966697 25.840065317720885 25.079390137714817 92-93 24.0289980908028 25.32754818626266 25.742391577825686 24.901062145108853 94-95 23.927412766259753 25.023878028661528 25.74628706521267 25.30242213986605 96-97 23.735398386385413 25.077084285067308 25.809199990922554 25.37831733762473 98-99 23.81722923306134 25.060630876853708 25.625154167201114 25.496985722883842 100-101 23.74735450388006 25.11186316926735 25.791971505108457 25.348810821744127 102-103 23.511950571677776 25.09037915832929 25.922390351136844 25.475279918856092 104-105 23.82357439209089 25.049604593913262 25.744907574135556 25.381913439860288 106-107 23.730092051522014 25.003145015557344 25.783602209552264 25.483160723368382 108-109 23.712447939946305 25.01743968631259 25.802052900217365 25.468059473523745 110-111 23.48856380446456 25.277339396722986 25.754490932295493 25.47960586651696 112-113 23.4261887636148 25.386108290881893 25.775151922376764 25.41255102312654 114-115 23.454660266498276 25.384034770082337 25.656255704158298 25.505049259261085 116-117 23.275291749609643 25.098631225040023 25.840912286414124 25.785164738936206 118-119 23.32001965477842 25.06373989387209 25.802372781683957 25.813867669665534 120-121 23.280762358076622 25.24023495121299 25.789773327136906 25.689229363573485 122-123 23.104542467602432 25.313862232141627 25.70034395590734 25.881251344348605 124-125 22.9675430076013 25.194057032694023 25.751228735699726 26.08717122400495 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 9.0 1 8.0 2 8.5 3 23.0 4 28.5 5 33.5 6 80.5 7 228.5 8 411.0 9 491.0 10 444.5 11 374.5 12 396.0 13 449.5 14 468.5 15 508.5 16 558.5 17 596.0 18 655.5 19 672.0 20 709.5 21 759.5 22 809.0 23 942.0 24 1070.5 25 1270.5 26 1571.5 27 1966.0 28 2466.5 29 3064.5 30 3889.5 31 4927.0 32 6170.0 33 7437.5 34 8693.5 35 10268.5 36 12237.0 37 14224.0 38 16288.5 39 18642.0 40 20799.0 41 22782.0 42 25105.0 43 28474.5 44 31958.0 45 35099.0 46 37672.0 47 40126.0 48 43562.5 49 46538.0 50 48746.5 51 49331.0 52 48396.0 53 47909.5 54 47399.0 55 45355.0 56 41871.5 57 38387.0 58 35431.0 59 31823.0 60 26945.5 61 22340.5 62 18261.0 63 13884.0 64 10123.0 65 7680.5 66 5879.5 67 4502.5 68 3542.5 69 2638.5 70 1988.5 71 1498.5 72 1090.5 73 849.0 74 629.0 75 437.5 76 271.5 77 143.0 78 78.0 79 40.0 80 25.0 81 18.0 82 10.0 83 5.5 84 5.0 85 5.5 86 3.0 87 1.0 88 1.0 89 0.0 90 2.0 91 2.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026245294840234232 2 0.0018746639171595882 3 0.03029062224042071 4 0.16566109036373414 5 0.02239730048395929 6 0.06373857318342599 7 0.04864259532419352 8 0.04143993922142248 9 0.030685288328243784 10-11 0.05643725055869918 12-13 0.052490589680468464 14-15 0.053378588378070385 16-17 0.04602793249236568 18-19 0.03369461724789471 20-21 0.021459968525379496 22-23 0.0029599956586730336 24-25 0.0042919937050759 26-27 0.0029599956586730336 28-29 0.003946660878230712 30-31 0.001973330439115356 32-33 0.007942655017439307 34-35 0.003847994356274944 36-37 0.005623991751478765 38-39 0.0018746639171595882 40-41 0.003058662180628802 42-43 0.003255995224540337 44-45 0.004439993488009551 46-47 0.004193327183120131 48-49 0.0015786643512922848 50-51 0.0014799978293365168 52-53 9.373319585797941E-4 54-55 0.004587993270943203 56-57 7.893321756461424E-4 58-59 6.906656536903746E-4 60-61 9.86665219557678E-5 62-63 0.0011839982634692136 64-65 0.0 66-67 0.0031573287025845696 68-69 3.946660878230712E-4 70-71 4.93332609778839E-5 72-73 0.0021706634830268912 74-75 0.004390660227031667 76-77 0.0017759973952038205 78-79 1.973330439115356E-4 80-81 0.0 82-83 0.004883992836810506 84-85 0.0016773308732480525 86-87 0.0 88-89 0.0012333315244470976 90-91 7.399989146682584E-4 92-93 0.0 94-95 0.003058662180628802 96-97 0.0013813313073807493 98-99 4.93332609778839E-4 100-101 0.0 102-103 6.906656536903746E-4 104-105 0.0 106-107 6.413323927124907E-4 108-109 0.0021706634830268912 110-111 4.93332609778839E-5 112-113 3.946660878230712E-4 114-115 0.0 116-117 0.0017266641342259363 118-119 0.0020719969610711237 120-121 0.0027626626147614986 122-123 0.0012826647854249813 124-125 0.09772918999718802 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1013515.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.27743253536765 #Duplication Level Percentage of deduplicated Percentage of total 1 82.41996838739757 54.62583894362877 2 10.08541893693995 13.36871346367914 3 3.080595694496204 6.125219199321486 4 1.4169399178853008 3.7564455925724958 5 0.7957455032281713 2.6369984452763653 6 0.5002437652097388 1.9892923439956054 7 0.33819683717447985 1.5690372641654415 8 0.2574706177136608 1.3651593196285272 9 0.18988975769093935 1.132686504406669 >10 0.8639034637945989 9.679053994140453 >50 0.037458710869136345 1.6845677776741514 >100 0.013124420319058274 1.4245814585881678 >500 7.457052007885611E-4 0.3241188286034073 >1k 2.982820803154244E-4 0.318286864319296 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1679 0.16566109036373414 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1541 0.1520451103338382 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 9.86665219557678E-5 0.0 0.0 0.0 0.0 12-13 9.86665219557678E-5 0.0 0.0 0.0 0.0 14-15 9.86665219557678E-5 0.0 0.0 0.0 9.86665219557678E-5 16-17 9.86665219557678E-5 0.0 0.0 0.0 2.959995658673034E-4 18-19 9.86665219557678E-5 0.0 0.0 0.0 2.959995658673034E-4 20-21 9.86665219557678E-5 0.0 0.0 0.0 2.959995658673034E-4 22-23 9.86665219557678E-5 0.0 0.0 0.0 4.4399934880095507E-4 24-25 9.86665219557678E-5 0.0 0.0 2.959995658673034E-4 5.919991317346068E-4 26-27 9.86665219557678E-5 0.0 0.0 9.373319585797941E-4 6.906656536903745E-4 28-29 9.86665219557678E-5 0.0 0.0 0.004390660227031667 6.906656536903745E-4 30-31 9.86665219557678E-5 0.0 0.0 0.017759973952038204 6.906656536903745E-4 32-33 9.86665219557678E-5 0.0 0.0 0.0404532740018648 6.906656536903745E-4 34-35 9.86665219557678E-5 0.0 0.0 0.06753723427872306 6.906656536903745E-4 36-37 9.86665219557678E-5 0.0 0.0 0.10281051587791004 6.906656536903745E-4 38-39 9.86665219557678E-5 0.0 0.0 0.16319442731483993 6.906656536903745E-4 40-41 9.86665219557678E-5 0.0 0.0 0.23975964835251576 6.906656536903745E-4 42-43 9.86665219557678E-5 0.0 0.0 0.30497821936527825 6.906656536903745E-4 44-45 9.86665219557678E-5 0.0 0.0 0.38055677518339637 6.906656536903745E-4 46-47 9.86665219557678E-5 0.0 0.0 0.4604766579675683 7.893321756461424E-4 48-49 9.86665219557678E-5 0.0 0.0 0.5457245329373517 0.0010853317415134458 50-51 9.86665219557678E-5 0.0 0.0 0.6334390709560292 0.0011839982634692136 52-53 9.86665219557678E-5 0.0 0.0 0.7280602655116106 0.0011839982634692136 54-55 9.86665219557678E-5 0.0 0.0 0.8342254431360168 0.0011839982634692136 56-57 9.86665219557678E-5 0.0 0.0 0.9433999496800738 0.0011839982634692136 58-59 9.86665219557678E-5 0.0 0.0 1.0649571047295798 0.0012333315244470976 60-61 9.86665219557678E-5 0.0 0.0 1.184195596513125 0.0012826647854249813 62-63 9.86665219557678E-5 0.0 0.0 1.3162607361509204 0.001381331307380749 64-65 9.86665219557678E-5 0.0 0.0 1.4432445499079933 0.001381331307380749 66-67 9.86665219557678E-5 0.0 0.0 1.5902083343611095 0.001381331307380749 68-69 9.86665219557678E-5 0.0 0.0 1.7454107733975324 0.001381331307380749 70-71 9.86665219557678E-5 0.0 0.0 1.9098385322368194 0.001381331307380749 72-73 9.86665219557678E-5 0.0 0.0 2.0632649738780384 0.001430664568358633 74-75 9.86665219557678E-5 0.0 0.0 2.226952733802657 0.0015786643512922848 76-77 9.86665219557678E-5 0.0 0.0 2.424631110541038 0.0016773308732480525 78-79 9.86665219557678E-5 0.0 0.0 2.6386387966630984 0.0016773308732480525 80-81 9.86665219557678E-5 0.0 4.93332609778839E-5 2.8454931599433655 0.0016773308732480525 82-83 9.86665219557678E-5 0.0 9.86665219557678E-5 3.0644835054241923 0.0017759973952038203 84-85 9.86665219557678E-5 0.0 9.86665219557678E-5 3.29136717266148 0.0017759973952038203 86-87 9.86665219557678E-5 0.0 9.86665219557678E-5 3.5394148088582806 0.0017759973952038203 88-89 9.86665219557678E-5 0.0 9.86665219557678E-5 3.829691716452149 0.0017759973952038203 90-91 9.86665219557678E-5 0.0 9.86665219557678E-5 4.1258886153633645 0.0017759973952038203 92-93 9.86665219557678E-5 0.0 9.86665219557678E-5 4.397862883134438 0.0017759973952038203 94-95 9.86665219557678E-5 0.0 9.86665219557678E-5 4.6838477970232315 0.0019733304391153558 96-97 9.86665219557678E-5 0.0 9.86665219557678E-5 5.008016654908906 0.0020719969610711237 98-99 9.86665219557678E-5 0.0 9.86665219557678E-5 5.389116095963059 0.0021706634830268917 100-101 9.86665219557678E-5 0.0 9.86665219557678E-5 5.788123510752184 0.0022199967440047754 102-103 9.86665219557678E-5 0.0 9.86665219557678E-5 6.188709589892602 0.002269330004982659 104-105 9.86665219557678E-5 0.0 9.86665219557678E-5 6.59274899730147 0.002269330004982659 106-107 9.86665219557678E-5 0.0 9.86665219557678E-5 7.037981677626873 0.002269330004982659 108-109 9.86665219557678E-5 0.0 9.86665219557678E-5 7.521447635210135 0.0024173297879163114 110-111 9.86665219557678E-5 0.0 9.86665219557678E-5 8.03515488177284 0.002466663048894195 112-113 9.86665219557678E-5 0.0 9.86665219557678E-5 8.54195547179864 0.002466663048894195 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3385 0.0 42.636185 1 GGTATCA 2285 0.0 37.583565 1 TCAACGC 4285 0.0 33.255333 4 TATCAAC 4395 0.0 32.834858 2 CAACGCA 4375 0.0 32.410904 5 GTACATG 6395 0.0 31.987125 1 ATCAACG 4470 0.0 31.883726 3 AACGCAG 4535 0.0 31.400337 6 CATGGGG 5155 0.0 30.65007 4 ACATGGG 6455 0.0 30.578094 3 TACATGG 6580 0.0 30.359707 2 ATGGGGG 2895 0.0 29.635084 5 ACGCAGA 5175 0.0 27.630777 7 CGCAGAG 5395 0.0 26.502726 8 TGGGGGG 1975 0.0 25.944485 6 GCAGAGT 5890 0.0 24.17427 9 GGGTTAG 285 3.1141099E-9 23.0181 1 GTAAGTA 560 0.0 22.361965 2 GAGTACT 2485 0.0 22.276207 12-13 AGAGTAC 5460 0.0 22.150023 10-11 >>END_MODULE