FastQCFastQC Report
Mon 15 Aug 2016
SRR2921605_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921605_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225257
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14080.11491466688213167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34750.036.497571
GTACATG77600.032.91811
TACATGG79850.031.6831952
CATGGGG62000.031.299754
ATGGGGG36850.030.8539525
TCAACGC41050.030.1623464
CAACGCA41650.029.7278355
ACATGGG84350.029.428353
GGTATCA24400.029.2840141
AACGCAG44150.028.310686
ATCAACG44150.028.0444933
TATCAAC45250.027.6258492
ACGCAGA51350.024.3291917
GTACACT9550.023.6861884
TACACTG12900.023.072535
CGCAGAG54350.022.7673598
GTCTCGT4750.022.5438339
GGTCTCG5050.022.3826278
AGTACAC9950.022.1357173
GTACCGT1903.297364E-521.928366
Produced by FastQC (version 0.11.5)