##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921605_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225257
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.20395149752256	31.0	30.0	34.0	23.0	34.0
2	29.234445508166857	31.0	30.0	34.0	22.0	34.0
3	29.390228335769557	31.0	31.0	34.0	23.0	34.0
4	32.77415023950078	37.0	35.0	37.0	24.0	37.0
5	32.85596001491932	37.0	35.0	37.0	25.0	37.0
6	32.64714994486871	37.0	35.0	37.0	23.0	37.0
7	32.54842453460784	37.0	35.0	37.0	22.0	37.0
8	32.593537519067425	37.0	35.0	37.0	22.0	37.0
9	34.17189536562533	39.0	35.0	39.0	19.0	39.0
10-11	34.22633129212892	39.0	35.0	39.0	21.5	39.0
12-13	34.14845130450183	39.0	35.0	39.0	18.5	39.0
14-15	35.314912708109404	40.0	36.0	41.0	18.0	41.0
16-17	35.2583262123783	40.0	36.0	41.0	17.5	41.0
18-19	35.193361882445885	40.0	36.0	41.0	17.5	41.0
20-21	35.244854344843574	40.0	36.0	41.0	18.0	41.0
22-23	35.166342244933105	40.0	36.0	41.0	18.0	41.0
24-25	35.162062326515986	40.0	36.0	41.0	18.0	41.0
26-27	35.12259713676396	40.0	36.0	41.0	18.0	41.0
28-29	35.05065875975407	40.0	36.0	41.0	18.0	41.0
30-31	34.93580530451979	40.0	36.0	41.0	16.5	41.0
32-33	34.83238536894709	40.0	35.0	41.0	16.0	41.0
34-35	34.675857391551325	40.0	35.0	41.0	13.5	41.0
36-37	34.556990084529204	40.0	35.0	41.0	9.0	41.0
38-39	34.42913649952622	40.0	35.0	41.0	9.0	41.0
40-41	34.305940712846365	40.0	35.0	41.0	7.5	41.0
42-43	34.00659698332676	39.5	34.5	41.0	2.0	41.0
44-45	33.559570767602224	39.0	33.5	40.5	2.0	41.0
46-47	33.25789936315402	38.0	33.0	40.0	2.0	41.0
48-49	33.02998677012251	38.0	33.0	40.0	2.0	41.0
50-51	32.808483036620075	38.0	33.0	40.0	2.0	41.0
52-53	32.60084700597507	38.0	32.0	40.0	2.0	41.0
54-55	32.3560644827983	38.0	32.0	40.0	2.0	41.0
56-57	32.10462213233632	37.0	32.0	40.0	2.0	41.0
58-59	32.10947091100071	37.0	32.0	40.0	2.0	41.0
60-61	31.895398271546295	37.0	32.0	40.0	2.0	41.0
62-63	31.593908869731003	36.0	31.0	39.5	2.0	41.0
64-65	31.302361463758217	36.0	31.0	39.0	2.0	41.0
66-67	30.988565664183106	35.0	31.0	39.0	2.0	40.5
68-69	30.661943983996828	35.0	31.0	38.0	2.0	40.0
70-71	30.30416067812712	35.0	30.0	37.5	2.0	40.0
72-73	29.970725325380716	35.0	30.0	37.0	2.0	39.0
74-75	29.615376202706862	35.0	30.0	36.0	2.0	39.0
76-77	29.32601323640673	35.0	30.0	36.0	2.0	38.5
78-79	29.06269092933156	34.5	29.0	35.5	2.0	37.0
80-81	28.804775651149107	34.0	29.0	35.0	2.0	37.0
82-83	28.581055647917132	34.0	29.0	35.0	2.0	36.5
84-85	28.37235453459968	34.0	29.0	35.0	2.0	36.0
86-87	28.201285934297864	34.0	29.0	35.0	2.0	36.0
88-89	28.0372232111304	34.0	29.0	35.0	2.0	35.0
90-91	27.852356689249685	34.0	28.0	35.0	2.0	35.0
92-93	27.75767492044526	34.0	28.0	35.0	2.0	35.0
94-95	27.627547934841424	34.0	27.0	35.0	2.0	35.0
96-97	27.5383058411419	34.0	27.0	35.0	2.0	35.0
98-99	27.43318911869102	34.0	27.0	35.0	2.0	35.0
100-101	27.318684569849427	34.0	27.0	35.0	2.0	35.0
102-103	26.431158932370924	32.5	25.0	34.0	2.0	35.0
104-105	27.367310694817498	34.0	27.0	35.0	2.0	35.0
106-107	27.6589682817564	34.0	29.0	35.0	2.0	35.0
108-109	27.667216347264286	34.0	29.0	35.0	2.0	35.0
110-111	27.602822509889762	34.0	29.0	35.0	2.0	35.0
112-113	27.517676291586174	34.0	28.5	35.0	2.0	35.0
114-115	27.389898609026513	34.0	27.0	35.0	2.0	35.0
116-117	27.25775898444163	34.0	27.0	35.0	2.0	35.0
118-119	26.970318063883738	34.0	27.0	35.0	2.0	35.0
120-121	26.817914527319573	34.0	25.0	35.0	2.0	35.0
122-123	26.528573189135017	34.0	25.0	35.0	2.0	35.0
124-125	25.710777820489906	33.0	22.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75887.0
3	12212.0
4	6394.0
5	5422.0
6	5764.0
7	5806.0
8	5865.0
9	6152.0
10	6541.0
11	6648.0
12	6674.0
13	6881.0
14	7112.0
15	6333.0
16	5195.0
17	4802.0
18	4447.0
19	4133.0
20	4062.0
21	4197.0
22	4558.0
23	6185.0
24	8754.0
25	10427.0
26	11763.0
27	13515.0
28	15971.0
29	19213.0
30	23464.0
31	29735.0
32	38953.0
33	52825.0
34	81201.0
35	128303.0
36	231196.0
37	293560.0
38	65072.0
39	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.40745433290447	18.056707528131717	12.123495451250335	25.41234268771348
2	16.32849460785754	19.911828757440112	37.83356025062117	25.926116384081183
3	19.955336117752015	23.730544234955527	28.825834529784494	27.488285117507967
4	13.56861483910885	15.393179736742866	33.92663866383047	37.111566760317814
5	14.3783503160225	38.026040939667986	32.99800582986619	14.597602914443325
6	33.86583662165143	33.80121402253213	16.54003578343668	15.792913572379764
7	29.41083275895298	32.34866574983585	19.96589650700526	18.274604984205904
8	28.375998164333023	34.57455886423822	18.73221391399639	18.317229057432368
9	28.18529225197748	16.305812874157393	18.07115409263796	37.43774078122717
10-11	26.56954059876171	25.92389804294321	26.020297339363086	21.48626401893199
12-13	26.080337672737098	23.625827132095846	26.36794884300479	23.92588635216227
14-15	24.074584321682952	24.833261286370963	23.715002626754693	27.3771517651914
16-17	24.451875958308772	27.663139056215886	24.285605814433865	23.59937917104148
18-19	24.317867178789367	27.05342055423075	25.691624891351523	22.937087375628366
20-21	25.026553860045443	25.689971743917106	25.858438739163127	23.42503565687433
22-23	25.33415307540035	25.185676885274077	25.448378090515362	24.03179194881021
24-25	24.794444385480357	25.621606219858577	25.421336962618312	24.162612432042753
26-27	24.26527266076141	25.900281871390867	25.802745551461943	24.031699916385776
28-29	24.52717182509532	26.006090578057833	25.73177368591022	23.734963910936628
30-31	25.018113302123346	25.609576395683504	25.552226109523946	23.820084192669206
32-33	24.37355724779518	25.93710773978516	25.60205245852119	24.08728255389847
34-35	24.455899572297135	26.146460877190975	25.763259360389064	23.634380190122826
36-37	24.67113855114861	26.055760340076528	25.570304031552414	23.70279707722245
38-39	24.696996289750487	26.212157699890206	25.459903590656214	23.6309424197031
40-41	24.546058766181275	26.05967251236611	25.493583012579222	23.90068570887339
42-43	24.39969354564182	25.83008599742771	25.476610405815286	24.293610051115184
44-45	24.302759737609424	25.8860498071906	25.739728532199656	24.071461923000317
46-47	24.24093006017374	25.868461892007282	25.712047727545468	24.17856032027351
48-49	24.176138624975763	26.13117531201845	25.71130001734853	23.981386045657256
50-51	24.190758818595025	26.08520304760194	25.68953728781388	24.034500845989154
52-53	24.256875101790243	26.02719876108774	25.501869266836767	24.21405687028525
54-55	24.03579192616303	25.781305486098017	25.674364004313578	24.508538583425377
56-57	24.12032426078012	25.972904795336916	25.38675363691283	24.52001730697014
58-59	24.1215641217682	26.129102697275382	25.558015807743878	24.19131737321255
60-61	24.494227557189717	26.013789860550318	25.566590854543588	23.92539172771638
62-63	24.28258476428616	25.940543057975685	25.703598576455516	24.073273601282644
64-65	24.095272186714155	25.72752308069703	25.598257299720693	24.57894743286812
66-67	24.211084473874667	25.97793195898256	25.42690446148992	24.38407910565286
68-69	24.00125554812676	25.87049568027769	25.555547392043675	24.572701379551873
70-71	23.929560915224705	25.831556651187555	25.76096568764744	24.477916745940306
72-73	24.141292102728208	26.137283874022433	25.396945256249083	24.324478767000276
74-75	23.823846782974652	26.06077845924296	25.627317085911955	24.488057671870433
76-77	23.803805879260636	25.73466269186941	25.724867189311144	24.736664239558813
78-79	23.900319649483585	25.753804996263067	25.739885896032895	24.605989458220453
80-81	24.09854238638516	26.025374362457303	25.48538141542641	24.390701835731125
82-83	24.051398645642422	25.948887083804443	25.72095662218812	24.278757648365016
84-85	24.07185051983554	25.919537931220255	25.387499295975264	24.621112252968942
86-87	23.821642064568163	26.091380544655607	25.583556661015113	24.503420729761125
88-89	23.827224944967973	25.789193762941657	25.751070333645316	24.632510958445053
90-91	24.05454253058599	25.63806695407061	25.71472070361448	24.59266981172892
92-93	24.228167513840113	25.60538381956532	25.82225478052732	24.344193886067245
94-95	24.08675174780937	25.582052150631984	25.749588810754997	24.581607290803646
96-97	23.77041017482366	25.571990991483702	25.809203281790406	24.84839555190223
98-99	23.68469918170306	25.672673877387275	25.6536968648751	24.988930076034563
100-101	23.66783198803063	25.895641839580712	25.76904271965274	24.66748345273592
102-103	23.40277055111073	25.856150896888895	25.820320231470994	24.92075832052938
104-105	23.37109290830618	25.935514622823224	25.744934737611903	24.948457731258692
106-107	23.387137582391752	25.85733943606335	25.936670573371508	24.81885240817339
108-109	23.249965206027255	25.849248429781678	25.96540519979087	24.935381164400194
110-111	23.23094935239245	26.32303617126597	25.804811381928687	24.64120309441289
112-113	23.078238371571054	26.415326102037984	25.83029308782841	24.676142438562557
114-115	23.162082211639714	26.514470051800615	25.69525958262386	24.628188153935817
116-117	22.989334619675983	26.336886283555298	25.82296137952087	24.850817717247846
118-119	22.94913038798704	26.021245223671517	25.941689198673572	25.087935189667864
120-121	22.895831471453974	26.364089335930373	25.95478193872958	24.785297253886075
122-123	22.84037422658452	26.22352880684466	25.956847884548996	24.97924908202183
124-125	22.652485341274133	26.157602334752365	25.992009802095435	25.197902521878067
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1163.0
1	881.5
2	475.0
3	297.5
4	216.0
5	187.0
6	204.0
7	248.5
8	305.0
9	355.5
10	358.0
11	348.0
12	383.0
13	416.5
14	459.0
15	496.5
16	542.0
17	620.5
18	707.0
19	769.5
20	846.0
21	971.0
22	1077.0
23	1213.5
24	1463.5
25	1804.0
26	2377.0
27	3156.5
28	3834.5
29	4842.0
30	6251.0
31	7859.0
32	9831.0
33	11930.5
34	14280.5
35	16493.0
36	18999.0
37	21820.0
38	24089.5
39	26157.0
40	28108.0
41	30089.0
42	32050.0
43	34993.0
44	38117.5
45	40661.5
46	43669.5
47	46848.5
48	50010.5
49	52566.5
50	54733.0
51	55288.5
52	53837.5
53	52947.0
54	52761.0
55	51362.5
56	48252.0
57	44485.5
58	41114.0
59	36834.5
60	31295.5
61	26205.5
62	21448.0
63	16486.0
64	12227.5
65	9366.0
66	7204.5
67	5499.0
68	4228.5
69	3309.5
70	2637.5
71	1990.5
72	1477.0
73	1208.5
74	965.0
75	729.0
76	516.0
77	339.0
78	244.5
79	176.5
80	134.5
81	108.5
82	91.5
83	67.0
84	55.0
85	50.5
86	42.5
87	39.0
88	36.5
89	32.5
90	24.0
91	23.0
92	23.5
93	20.5
94	20.0
95	16.5
96	11.5
97	8.0
98	8.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024892736789098122
2	0.011507789794304378
3	0.008651246228342298
4	0.011671020855216497
5	0.015180488664827052
6	0.10014225586958492
7	0.06113003231158851
8	0.05174424630914168
9	0.027586049294148082
10-11	0.0541110966923674
12-13	0.013507370290477834
14-15	0.029504014259865482
16-17	0.036155679992034326
18-19	0.044929349516060715
20-21	0.03166682581695106
22-23	0.03929787791459261
24-25	0.033135905365160125
26-27	0.047745085316794764
28-29	0.04721458436883038
30-31	0.026810701754815522
32-33	0.03138117146035485
34-35	0.04692893001223417
36-37	0.009875479185183191
38-39	0.022199424284048162
40-41	0.043990770915816026
42-43	0.02272992523201255
44-45	0.017384107987140657
46-47	0.024811121258642065
48-49	0.030156938503513957
50-51	0.029340783198953363
52-53	0.02542323773706251
54-55	0.023505272771345113
56-57	0.02587212315457084
58-59	0.01832268658738534
60-61	0.02440304360636177
62-63	0.023382849475661025
64-65	0.021342461214259537
66-67	0.0296672453207776
68-69	0.02399496595408147
70-71	0.048642856151811416
72-73	0.02260750193632846
74-75	0.009712248124271072
76-77	0.01673118374349218
78-79	0.0262393930416231
80-81	0.01966934283991032
82-83	0.02628020080685113
84-85	0.012895253812057389
86-87	0.01844510988306943
88-89	0.023546080536573144
90-91	0.021872962162223925
92-93	0.00852882293265821
94-95	0.012405560629321032
96-97	0.01583341290847553
98-99	0.007386205506273378
100-101	0.010610018959287724
102-103	0.004039968757574941
104-105	0.003999160992346911
106-107	0.0010610018959287724
108-109	0.015384527490967201
110-111	0.0013466562525249804
112-113	0.002203619322313604
114-115	0.0142827178298104
116-117	0.006366011375572635
118-119	0.2837363916304906
120-121	0.0013466562525249804
122-123	8.161553045605942E-4
124-125	0.31711714358701887
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1225257.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.96397815206662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.13002857881628	57.1781142426341
2	9.038863959674956	12.286343053497022
3	2.7803057419417607	5.668819161041856
4	1.2428534658058348	3.3787706318499215
5	0.7116672768329358	2.4183869627107195
6	0.44779685148075776	1.8260433258361464
7	0.30332645333227554	1.443069071305304
8	0.23277236881693592	1.2656108950943221
9	0.18245118879205702	1.1160097747993751
>10	0.8872920635946638	10.548375939559538
>50	0.03317293311623222	1.523550848840679
>100	0.008749950633600732	0.9563751773112006
>500	5.993059680967582E-4	0.2758318064142725
>1k	1.1986119361935163E-4	0.11469910910552147
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1408	0.11491466688213167	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	8.161553045605942E-5	0.0
12-13	0.0	0.0	0.0	8.161553045605942E-5	0.0
14-15	0.0	0.0	0.0	8.161553045605942E-5	2.0403882614014856E-4
16-17	0.0	0.0	0.0	8.161553045605942E-5	2.448465913681783E-4
18-19	0.0	0.0	0.0	1.6323106091211884E-4	2.448465913681783E-4
20-21	0.0	0.0	0.0	2.8565435659620795E-4	2.448465913681783E-4
22-23	0.0	0.0	0.0	4.080776522802971E-4	2.8565435659620795E-4
24-25	0.0	0.0	0.0	9.793863654727131E-4	3.264621218242377E-4
26-27	0.0	0.0	0.0	0.0024484659136817825	3.264621218242377E-4
28-29	0.0	0.0	0.0	0.006529242436484754	3.264621218242377E-4
30-31	0.0	0.0	0.0	0.02256669417110043	3.264621218242377E-4
32-33	0.0	0.0	0.0	0.052397170552790154	3.264621218242377E-4
34-35	0.0	0.0	0.0	0.09087889316282216	3.264621218242377E-4
36-37	0.0	0.0	0.0	0.14870349649094028	4.080776522802971E-4
38-39	0.0	0.0	0.0	0.23297153168682164	4.080776522802971E-4
40-41	0.0	0.0	0.0	0.32340153943213545	4.080776522802971E-4
42-43	0.0	0.0	0.0	0.40979157841987435	4.4888541750832684E-4
44-45	0.0	0.0	0.0	0.5127903778554214	4.896931827363566E-4
46-47	0.0	0.0	0.0	0.6266032350763961	4.896931827363566E-4
48-49	0.0	0.0	0.0	0.7413954786628438	4.896931827363566E-4
50-51	0.0	0.0	0.0	0.8497400953432628	4.896931827363566E-4
52-53	0.0	0.0	0.0	0.9626143739639929	5.305009479643863E-4
54-55	0.0	0.0	0.0	1.0927503372761795	6.121164784204456E-4
56-57	0.0	0.0	0.0	1.238842136792526	6.529242436484753E-4
58-59	0.0	0.0	0.0	1.3930138738240223	6.937320088765051E-4
60-61	0.0	0.0	0.0	1.5420438324367867	7.345397741045348E-4
62-63	0.0	0.0	0.0	1.690461674571131	7.345397741045348E-4
64-65	0.0	0.0	0.0	1.843409178645786	7.345397741045348E-4
66-67	0.0	0.0	0.0	2.0249629261452906	8.161553045605942E-4
68-69	0.0	0.0	0.0	2.2283896358070185	8.977708350166537E-4
70-71	0.0	0.0	0.0	2.449404492282027	8.977708350166537E-4
72-73	0.0	0.0	0.0	2.653810588309228	0.0010610018959287726
74-75	0.0	0.0	0.0	2.8642154258249493	0.0010610018959287726
76-77	0.0	0.0	0.0	3.1100822113238284	0.0010610018959287726
78-79	0.0	0.0	0.0	3.3825964675166107	0.0010610018959287726
80-81	0.0	0.0	0.0	3.657273535266479	0.0010610018959287726
82-83	0.0	0.0	0.0	3.9375004590873592	0.001142617426384832
84-85	0.0	0.0	0.0	4.221563312839674	0.001142617426384832
86-87	0.0	0.0	0.0	4.54929047538598	0.001142617426384832
88-89	0.0	0.0	0.0	4.913254933454777	0.001142617426384832
90-91	0.0	0.0	0.0	5.289053643439702	0.001142617426384832
92-93	0.0	0.0	0.0	5.671095941504517	0.001142617426384832
94-95	0.0	8.161553045605942E-5	0.0	6.055382666656873	0.0012242329568408913
96-97	0.0	8.161553045605942E-5	0.0	6.474355992253054	0.0012242329568408913
98-99	0.0	1.2242329568408911E-4	0.0	6.945971334993393	0.0012242329568408913
100-101	0.0	1.6323106091211884E-4	0.0	7.451212276281629	0.0012242329568408913
102-103	0.0	1.6323106091211884E-4	0.0	7.964614770615471	0.0013058484872969507
104-105	0.0	1.6323106091211884E-4	0.0	8.500706382416098	0.0013058484872969507
106-107	0.0	1.6323106091211884E-4	0.0	9.061690731005822	0.0013058484872969507
108-109	0.0	1.6323106091211884E-4	0.0	9.665604848615434	0.0013466562525249804
110-111	0.0	1.6323106091211884E-4	0.0	10.309102498496234	0.0014690795482090696
112-113	0.0	1.6323106091211884E-4	0.0	10.952110455194298	0.0014690795482090696
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3475	0.0	36.49757	1
GTACATG	7760	0.0	32.9181	1
TACATGG	7985	0.0	31.683195	2
CATGGGG	6200	0.0	31.29975	4
ATGGGGG	3685	0.0	30.853952	5
TCAACGC	4105	0.0	30.162346	4
CAACGCA	4165	0.0	29.727835	5
ACATGGG	8435	0.0	29.42835	3
GGTATCA	2440	0.0	29.284014	1
AACGCAG	4415	0.0	28.31068	6
ATCAACG	4415	0.0	28.044493	3
TATCAAC	4525	0.0	27.625849	2
ACGCAGA	5135	0.0	24.329191	7
GTACACT	955	0.0	23.686188	4
TACACTG	1290	0.0	23.07253	5
CGCAGAG	5435	0.0	22.767359	8
GTCTCGT	475	0.0	22.543833	9
GGTCTCG	505	0.0	22.382627	8
AGTACAC	995	0.0	22.135717	3
GTACCGT	190	3.297364E-5	21.92836	6
>>END_MODULE