FastQCFastQC Report
Mon 15 Aug 2016
SRR2921605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225257
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29410.24003127507127076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24810.20248813106148342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16270.13278846805200867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.044.6106531
GTATCAA45000.044.541491
TCAACGC57200.034.5142824
CAACGCA58600.033.7597165
TATCAAC59250.033.5241932
ATCAACG59450.033.2107433
CATGGGG59950.033.030554
GTACATG76100.032.8448561
AACGCAG60450.032.834516
TACATGG78000.031.430382
ACATGGG82000.029.9699363
ACGCAGA70400.028.1045767
ATGGGGG35950.027.0176185
CGCAGAG73600.026.9614058
ATGGGGA18050.026.7402925
AGTACTT36400.024.77924312-13
GAGTACT35000.024.15444812-13
GCAGAGT80700.024.0724419
GTACTTT36700.023.10960614-15
AGAGTAC73650.022.00757810-11
Produced by FastQC (version 0.11.5)