FastQCFastQC Report
Mon 15 Aug 2016
SRR2921604_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921604_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506369
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.049.270651
GTATCAA31250.040.007771
GTACATG86150.037.3866921
TACATGG87200.036.0393522
ACATGGG90200.034.4424973
CATGGGG75750.034.2558364
ATGGGGG45050.033.1563225
TCAACGC37750.031.846754
CAACGCA38850.030.4824225
TATCAAC40550.030.2368072
AACGCAG41850.029.0063616
ATCAACG41850.028.8689693
TAGAGAG10450.028.478252
GTATAGG4850.024.5506671
ACGCAGA49750.024.0351127
TATCACG1300.002049330622.8906084
TATAGGG3404.3655746E-1122.7574712
CGCAGAG53500.022.0160888
GAGTACA60750.021.7561341
GTACTAG2204.157375E-621.6492231
Produced by FastQC (version 0.11.5)