FastQCFastQC Report
Mon 15 Aug 2016
SRR2921604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506369
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.1690820774989395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20820.1382131469779317No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27150.049.8727651
GTATCAA41750.047.00531
TATACCG2150.038.7963875
TCAACGC56450.034.754364
CAACGCA57700.034.075315
TATCAAC59000.033.5621762
AACGCAG60200.032.7722746
ATCAACG60050.032.576953
GTACATG88000.032.2650031
TACATGG89850.031.0664632
CATGGGG74000.029.6546944
ACATGGG93000.029.56433
ATGGGGG42850.029.338195
ACGCAGA72750.027.0359447
CGCAGAG77100.025.5088718
ACTGCGT2851.1459633E-1025.0938938
TGGGGGG30700.023.8804116
GACCGCG2552.4350811E-823.373327
GCAGAGT84800.023.1223379
GTGGTAT18150.022.67671
Produced by FastQC (version 0.11.5)