FastQCFastQC Report
Mon 15 Aug 2016
SRR2921603_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921603_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455863
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33000.035.3485831
GTACATG86450.034.6284331
TATAACG1408.059578E-834.0041662
ACATGGG89850.033.1147963
TACATGG89850.032.717422
CATGGGG75250.032.2644164
ATGGGGG46200.031.1694135
GGTATCA22050.031.0398161
TCAACGC36900.030.8018764
CAACGCA37750.030.2649175
TATCAAC39000.029.4484792
AACGCAG39700.028.7743996
ATCAACG40700.028.3646553
GAGTCCG4900.024.2886893
ACGCAGA47150.024.2195367
TATACTG6000.023.8020975
TGGGGGG34600.023.5580846
CGCAGAG50700.022.5229178
AGTCCGT3401.0677468E-921.0025734
TAGGCGT2556.3802327E-721.0025734
Produced by FastQC (version 0.11.5)