FastQCFastQC Report
Mon 15 Aug 2016
SRR2921603_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921603_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455863
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.10152054142457086No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1204.0927262E-1044.7218742
GTATCAA37850.043.9659771
GGTATCA23650.037.3257871
TCAACGC45950.035.942494
CAACGCA47500.034.7337575
AACGCAG49600.033.9941946
TATCAAC48850.033.812242
ATCAACG49300.033.3815083
GTACATG80700.032.8161621
TACATGG83100.031.5726032
ACATGGG84700.030.5527343
CATGGGG71300.029.6024464
ATGGGGG41250.029.022695
ACGCAGA58750.028.3945667
CGCAGAG62800.026.3718348
GCAGAGT69450.023.846679
TGGGGGG27700.023.4448136
AGAGTAC64050.021.71815510-11
GAGTACT24200.020.78300312-13
TATACCG2055.891334E-520.338075
Produced by FastQC (version 0.11.5)