FastQCFastQC Report
Mon 15 Aug 2016
SRR2921602_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921602_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487308
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24300.042.865541
GGTATCA16450.041.6110271
GTACATG76400.038.9540941
TACATGG79000.037.5903932
TCAACGC27050.036.7411424
CAACGCA27300.036.4022335
ACATGGG82200.035.4754333
CATGGGG71400.034.2568174
ATCAACG30950.032.4959683
AACGCAG30750.032.3148166
TATCAAC31750.032.2394872
ATGGGGG44800.031.347885
ACGCAGA38100.025.9168347
GTATATA4150.024.3824481
CGCAGAG41100.024.3153728
GAGTACA51300.024.2496471
AGTACAT51200.023.5955162
TGGGGGG36150.023.5373786
GTACCGT2853.1996024E-922.965626
ATGGGGA25750.022.1854485
Produced by FastQC (version 0.11.5)