##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921602_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1487308
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.36176635908635	31.0	30.0	34.0	25.0	34.0
2	29.4127241970056	31.0	30.0	34.0	25.0	34.0
3	29.612557721736184	31.0	31.0	34.0	25.0	34.0
4	33.173688301279896	37.0	35.0	37.0	27.0	37.0
5	33.110925914470975	37.0	35.0	37.0	26.0	37.0
6	33.104430958483384	37.0	35.0	37.0	26.0	37.0
7	32.8633948045731	37.0	35.0	37.0	25.0	37.0
8	32.914291458124346	37.0	35.0	37.0	25.0	37.0
9	34.479478359559685	39.0	35.0	39.0	25.0	39.0
10-11	34.58217127857848	39.0	35.0	39.0	26.0	39.0
12-13	34.55027438835803	39.0	35.0	39.0	25.5	39.0
14-15	35.741721620538584	40.0	37.0	41.0	25.0	41.0
16-17	35.66231641327822	40.0	36.5	41.0	25.0	41.0
18-19	35.57274317088323	40.0	36.5	41.0	24.0	41.0
20-21	35.65353343086973	40.0	37.0	41.0	25.0	41.0
22-23	35.55103784824663	40.0	36.5	41.0	24.0	41.0
24-25	35.51828168745142	40.0	36.5	41.0	24.0	41.0
26-27	35.4808341648132	40.0	36.0	41.0	24.0	41.0
28-29	35.417464640814146	40.0	36.0	41.0	23.5	41.0
30-31	35.317874979493155	40.0	36.0	41.0	23.0	41.0
32-33	35.23117235972643	40.0	36.0	41.0	22.5	41.0
34-35	35.08155506458648	40.0	35.5	41.0	20.5	41.0
36-37	34.973012314866864	40.0	35.0	41.0	19.0	41.0
38-39	34.84696411234257	40.0	35.0	41.0	18.0	41.0
40-41	34.72817130009386	40.0	35.0	41.0	17.0	41.0
42-43	34.43417973950251	39.5	35.0	41.0	15.0	41.0
44-45	34.00105492608122	39.0	34.0	40.5	11.0	41.0
46-47	33.70923978086583	38.0	33.5	40.0	9.0	41.0
48-49	33.49516340932746	38.0	33.0	40.0	8.0	41.0
50-51	33.27047087758554	38.0	33.0	40.0	7.0	41.0
52-53	33.051731383143235	38.0	33.0	40.0	4.0	41.0
54-55	32.80135688102263	38.0	32.5	40.0	2.0	41.0
56-57	32.53825737506959	37.0	32.0	40.0	2.0	41.0
58-59	32.55115651902632	37.0	32.0	40.0	2.0	41.0
60-61	32.3367789321378	37.0	32.0	40.0	2.0	41.0
62-63	32.02760389912513	36.0	32.0	39.0	2.0	41.0
64-65	31.73712943116019	36.0	31.5	39.0	2.0	41.0
66-67	31.419188560809193	35.0	31.0	39.0	2.0	40.0
68-69	31.091181517210963	35.0	31.0	38.0	2.0	40.0
70-71	30.7421478940475	35.0	31.0	37.0	2.0	39.5
72-73	30.40775885021798	35.0	31.0	37.0	2.0	39.0
74-75	30.048331280407286	35.0	30.0	36.0	2.0	39.0
76-77	29.76591634012592	35.0	30.0	36.0	2.0	37.5
78-79	29.508394360818336	34.5	30.0	35.0	2.0	37.0
80-81	29.2642159525801	34.0	30.0	35.0	2.0	37.0
82-83	29.054636968267502	34.0	29.5	35.0	2.0	36.0
84-85	28.85125273312589	34.0	29.0	35.0	2.0	36.0
86-87	28.68770053008523	34.0	29.0	35.0	2.0	35.5
88-89	28.528128000387277	34.0	29.0	35.0	2.0	35.0
90-91	28.353508822651392	34.0	29.0	35.0	2.0	35.0
92-93	28.28107493538662	34.0	29.0	35.0	2.0	35.0
94-95	28.15314178367897	34.0	29.0	35.0	2.0	35.0
96-97	28.06732600107039	34.0	29.0	35.0	2.0	35.0
98-99	27.97472783041576	34.0	29.0	35.0	2.0	35.0
100-101	27.867540213593955	34.0	28.0	35.0	2.0	35.0
102-103	26.966760415462026	32.5	26.0	34.0	2.0	35.0
104-105	27.93437606736466	34.0	29.0	35.0	2.0	35.0
106-107	28.240144946440147	34.0	29.0	35.0	2.0	35.0
108-109	28.250055133166768	34.0	29.0	35.0	2.0	35.0
110-111	28.197980512442616	34.0	29.0	35.0	2.0	35.0
112-113	28.121843626202505	34.0	29.0	35.0	2.0	35.0
114-115	27.997534471676346	34.0	29.0	35.0	2.0	35.0
116-117	27.877380139150734	34.0	29.0	35.0	2.0	35.0
118-119	27.586690517364257	34.0	29.0	35.0	2.0	35.0
120-121	27.44736900494047	34.0	29.0	35.0	2.0	35.0
122-123	27.162843203963135	34.0	27.0	35.0	2.0	35.0
124-125	26.332474510995702	33.5	25.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82791.0
3	13153.0
4	6605.0
5	5625.0
6	5622.0
7	6164.0
8	6488.0
9	6840.0
10	7074.0
11	7137.0
12	7041.0
13	6840.0
14	6768.0
15	6333.0
16	5679.0
17	5252.0
18	4977.0
19	4894.0
20	4763.0
21	4961.0
22	5553.0
23	7335.0
24	9564.0
25	11930.0
26	14703.0
27	16719.0
28	19970.0
29	24450.0
30	29872.0
31	37726.0
32	49446.0
33	68449.0
34	104200.0
35	164745.0
36	293084.0
37	354648.0
38	69895.0
39	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.99333691101131	16.84945104162884	11.733102532753911	25.424109514605934
2	16.721727056023077	18.61056850160205	38.46875409758902	26.19895034478585
3	20.478770616144338	22.595052125359068	27.968899611614972	28.957277646881625
4	13.809464251852868	14.08105683742235	33.75090945524135	38.358569455483426
5	14.900573406757015	37.69495459885399	32.24184087306845	15.16263112132055
6	36.24838407282961	32.485301035202816	16.39164219923566	14.874672692731911
7	31.996641733059487	30.682396882301223	18.6540561231526	18.666905261486693
8	28.117905442902163	35.258019056716094	18.14195336385009	18.48212213653165
9	28.435972741279556	15.761259555717558	17.625965323345913	38.17680237965697
10-11	27.491298093400406	24.906439158630146	25.682438343346682	21.919824404622766
12-13	26.960546157247155	22.076466721694825	26.22018771213438	24.742799408923638
14-15	24.421708888107997	23.527702724287572	23.267026587150134	28.7835618004543
16-17	25.334640823557425	26.624375007566492	23.85345751800153	24.187526650874553
18-19	25.29880645574631	26.037284427020396	25.021414784962598	23.642494332270694
20-21	25.753120072474662	25.007053433584563	25.04138742972208	24.198439064218697
22-23	25.844180127632555	24.470764772984083	24.834607685933516	24.850447413449846
24-25	25.361313153086744	24.943680647496844	24.768072551499547	24.926933647916865
26-27	24.911377930245855	25.212524804089732	25.012847677664546	24.863249587999867
28-29	25.105673546097478	25.533648044534907	24.7935632635435	24.56711514582411
30-31	25.681851578979582	25.083603717872887	24.835642404410983	24.398902298736555
32-33	25.164159480258323	25.135441869536635	24.906339900160905	24.794058750044133
34-35	25.072275136545862	25.591452094600047	24.949720181881776	24.386552586972314
36-37	25.22347995303794	25.432670906521025	24.91799795314284	24.42585118729819
38-39	25.30337098297612	25.451017300274813	24.828253136601592	24.41735858014747
40-41	25.237589804540388	25.27283562733865	24.841746928266005	24.647827639854956
42-43	25.12408643766592	25.22980495538739	24.83406664906933	24.812041957877355
44-45	24.881687175343725	25.240416703232267	25.081377368063755	24.796518753360253
46-47	24.815412602179606	25.156947204540792	25.09736274895509	24.930277444324513
48-49	24.803024097139133	25.378129840327617	25.04380044617606	24.77504561635719
50-51	24.8249943421815	25.37386158470704	25.110390512216686	24.690753560894773
52-53	24.949804547938296	25.318313648018158	25.00528771384137	24.726594090202177
54-55	24.741133089569935	24.994703963318475	25.204527892796115	25.059635054315482
56-57	24.86672248618803	25.26092263616096	24.863595247942914	25.008759629708088
58-59	24.808242705623805	25.32766523473541	25.097543031910458	24.76654902773033
60-61	25.129408235992738	25.19494458832478	25.14817133737872	24.52747583830376
62-63	24.974864672743085	25.20389037701982	25.178132971429456	24.643111978807642
64-65	24.81974364811351	24.942038541889783	25.152296988626944	25.08592082136976
66-67	24.793293384177716	25.222276025798816	25.053803576592752	24.930627013430716
68-69	24.629629505089927	25.20567171747401	25.17086909708889	24.99382968034718
70-71	24.64878143444419	24.988035143549688	25.38298471289894	24.98019870910718
72-73	24.766567417118782	25.32746466441492	24.994510732233014	24.911457186233285
74-75	24.62517609007743	25.285123036112335	25.10390575356467	24.98579512024557
76-77	24.671338321048975	24.980742118558613	25.15111258606365	25.196806974328766
78-79	24.58804160541037	25.006254531172452	25.30926302791942	25.09644083549776
80-81	24.729687063411447	25.204930779646496	25.06182640322819	25.00355575371387
82-83	24.791402003159487	25.242761866396407	25.108998211776985	24.856837918667125
84-85	24.798765679903195	25.286414914051146	24.95648510608825	24.958334299957404
86-87	24.460751200955183	25.428004724804993	25.09993809873336	25.011305975506463
88-89	24.48011938323147	25.082381961780147	25.217084875613327	25.220413779375058
90-91	24.744368026652303	24.959196395967453	25.221872749244955	25.07456282813529
92-93	24.719014994160084	25.10951943242878	25.27318500328474	24.898280570126392
94-95	24.663843579213154	25.03610111337683	25.245899434654046	25.054155872755974
96-97	24.51305245695384	25.032521448689742	25.244074775291836	25.21035131906458
98-99	24.397741144765025	25.017826962974716	25.204114943819434	25.38031694844082
100-101	24.38253154836632	25.044194945940625	25.341405537127454	25.23186796856561
102-103	24.18676002045392	24.96161528140445	25.505708371617192	25.34591632652444
104-105	24.25210356378737	25.080921816145096	25.347725111918873	25.319249508148662
106-107	24.23680186970431	24.942613901488205	25.472166229407232	25.34841799940025
108-109	24.237799650193764	24.907925377359696	25.512655763632658	25.34161920881388
110-111	24.112798521336472	25.234436615882995	25.428581802191776	25.22418306058876
112-113	24.023346099435745	25.280076542824716	25.508985578966055	25.187591778773488
114-115	24.02781494895482	25.40961889942533	25.38396518357032	25.178600968049526
116-117	23.91657283907119	25.207779515180572	25.50134866312066	25.37429898262758
118-119	23.93631725004652	25.04716166979954	25.600696064444616	25.41582501570932
120-121	23.845240724660044	25.15510587877755	25.688357452696696	25.31129594386571
122-123	23.859691587748227	25.161316349480096	25.59115710064244	25.387834962129237
124-125	23.726018583184757	25.130734151466477	25.61721660946749	25.526030655881275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	261.0
1	225.0
2	164.0
3	126.0
4	98.0
5	74.5
6	85.0
7	115.0
8	132.5
9	143.0
10	149.0
11	164.5
12	173.5
13	178.0
14	197.5
15	232.0
16	273.0
17	287.0
18	310.0
19	359.5
20	406.0
21	458.0
22	525.0
23	672.0
24	813.0
25	993.0
26	1357.0
27	1816.5
28	2439.0
29	3333.0
30	4579.0
31	6093.5
32	8031.0
33	10110.5
34	12396.5
35	14919.0
36	17786.0
37	20988.5
38	24053.5
39	27361.0
40	30711.5
41	33990.0
42	37082.0
43	41375.0
44	46285.5
45	50610.0
46	54286.5
47	57912.0
48	62555.5
49	66183.0
50	68060.0
51	69492.0
52	69906.5
53	69367.5
54	68974.0
55	66817.0
56	63022.0
57	58307.0
58	53424.0
59	48384.5
60	41739.5
61	35445.0
62	29576.5
63	23097.0
64	17403.5
65	13379.0
66	10464.5
67	8241.5
68	6582.0
69	5184.0
70	4004.5
71	3050.0
72	2262.0
73	1803.0
74	1463.0
75	1090.5
76	816.0
77	588.0
78	411.5
79	281.5
80	195.0
81	141.0
82	108.0
83	81.0
84	65.5
85	53.0
86	46.0
87	40.5
88	36.0
89	27.0
90	22.0
91	22.0
92	19.5
93	15.5
94	10.5
95	12.5
96	12.5
97	7.5
98	5.5
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.020843026461230624
2	0.01028704209215576
3	0.008337210584492251
4	0.010354277661385537
5	0.015531416492078304
6	0.08868371581407482
7	0.05560381575302493
8	0.04659424947623492
9	0.024944396184246972
10-11	0.04763640079929645
12-13	0.01280837593827237
14-15	0.027264023322674254
16-17	0.03294542892259034
18-19	0.040442194891710394
20-21	0.029818974953405752
22-23	0.03630720738407915
24-25	0.03190327759952881
26-27	0.04424100455319275
28-29	0.04397206227627364
30-31	0.025717605230389403
32-33	0.027936379014972014
34-35	0.04222393747629946
36-37	0.010085335384466432
38-39	0.02074217310738596
40-41	0.04081199052247416
42-43	0.0228937113227388
44-45	0.017111452368978045
46-47	0.02306180024581324
48-49	0.030323241722629075
50-51	0.02874320584572933
52-53	0.024910778399632086
54-55	0.023330742522732345
56-57	0.02507886732270653
58-59	0.01828807483049913
60-61	0.023330742522732345
62-63	0.02400309821503011
64-65	0.022086884491981488
66-67	0.028104467938046458
68-69	0.023599684799651452
70-71	0.04454356461472674
72-73	0.021212822091994394
74-75	0.009614686399857998
76-77	0.017111452368978045
78-79	0.02595292972269362
80-81	0.018926812738182006
82-83	0.02400309821503011
84-85	0.011665371261366173
86-87	0.017649336922816256
88-89	0.022322208984285705
90-91	0.020641319753541297
92-93	0.008673388430641131
94-95	0.011127486707527964
96-97	0.014287558461327444
98-99	0.006353761292213852
100-101	0.009883628676777103
102-103	0.004202223076861013
104-105	0.004706489846084335
106-107	9.41297969216867E-4
108-109	0.013379878276725465
110-111	0.0012774758153657481
112-113	0.0024204804922719437
114-115	0.013043700430576585
116-117	0.006353761292213852
118-119	0.27647266067283977
120-121	0.0011766224615210837
122-123	8.740623999870909E-4
124-125	0.3108636543338703
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1487308.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.64335138002532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.42130295273442	55.59475205258623
2	9.176939998920956	12.231640738829967
3	2.9639088027128166	5.92574447392621
4	1.3583434508588805	3.620982395613781
5	0.7780469927329299	2.5925829563436324
6	0.5234094230898761	2.092905485915697
7	0.3492393268783839	1.629213542381576
8	0.2396749848741176	1.2778195387174456
9	0.18839843867158193	1.1299953013054542
>10	0.9482105423839916	10.900582109219807
>50	0.0418504066870291	1.8245100007880346
>100	0.010473288767239792	1.0891470366233456
>500	2.0139068780596514E-4	0.09012436774877179
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.723556922977622E-5
2	0.0	0.0	0.0	0.0	6.723556922977622E-5
3	0.0	0.0	0.0	0.0	6.723556922977622E-5
4	0.0	0.0	0.0	0.0	6.723556922977622E-5
5	0.0	0.0	0.0	0.0	6.723556922977622E-5
6	0.0	0.0	0.0	6.723556922977622E-5	1.3447113845955244E-4
7	0.0	0.0	0.0	6.723556922977622E-5	1.3447113845955244E-4
8	0.0	0.0	0.0	6.723556922977622E-5	1.3447113845955244E-4
9	0.0	0.0	0.0	6.723556922977622E-5	1.3447113845955244E-4
10-11	0.0	0.0	0.0	6.723556922977622E-5	1.3447113845955244E-4
12-13	0.0	0.0	0.0	6.723556922977622E-5	4.034134153786573E-4
14-15	0.0	0.0	0.0	6.723556922977622E-5	5.042667692233216E-4
16-17	0.0	0.0	0.0	1.0085335384466432E-4	6.051201230679859E-4
18-19	0.0	0.0	0.0	1.3447113845955244E-4	7.395912615275384E-4
20-21	0.0	0.0	0.0	2.3532449230421676E-4	0.0010085335384466432
22-23	0.0	0.0	0.0	3.6979563076376914E-4	0.0011093868922913076
24-25	0.0	0.0	0.0	8.740623999870908E-4	0.0011430046769061956
26-27	0.0	0.0	0.0	0.0013447113845955244	0.0011430046769061956
28-29	0.0	0.0	0.0	0.0030592183999548174	0.0011430046769061956
30-31	0.0	0.0	0.0	0.009749157538317552	0.0011430046769061956
32-33	0.0	0.0	0.0	0.026524432061146713	0.0013447113845955242
34-35	0.0	0.0	0.0	0.0460563649223967	0.0014791825230550768
36-37	0.0	0.0	0.0	0.07762346467577663	0.0014791825230550768
38-39	0.0	0.0	0.0	0.1289242039980959	0.001512800307669965
40-41	0.0	0.0	0.0	0.19037751427411134	0.0015464180922848529
42-43	0.0	0.0	0.0	0.24678815685789357	0.0016136536615146291
44-45	0.0	0.0	0.0	0.30938447181081524	0.0017145070153592934
46-47	0.0	0.0	0.0	0.3732918803637175	0.0017481247999741815
48-49	0.0	0.0	0.0	0.44341857907037413	0.0018153603692039578
50-51	0.0	0.0	0.0	0.5163355539000665	0.0020843026461230627
52-53	0.0	0.0	0.0	0.5898912666374416	0.0020843026461230627
54-55	0.0	6.723556922977622E-5	0.0	0.6739357281746619	0.002117920430737951
56-57	0.0	6.723556922977622E-5	0.0	0.7661829291579149	0.0022523915691975032
58-59	0.0	6.723556922977622E-5	0.0	0.869187821217932	0.0023532449230421674
60-61	0.0	6.723556922977622E-5	0.0	0.9785464745701631	0.0024204804922719437
62-63	0.0	6.723556922977622E-5	0.0	1.0908298751838892	0.0025549516307314963
64-65	0.0	6.723556922977622E-5	0.0	1.2015332399207157	0.0026894227691910484
66-67	0.0	6.723556922977622E-5	0.0	1.3297850882265139	0.0026894227691910484
68-69	0.0	6.723556922977622E-5	0.0	1.4727279084090181	0.0026894227691910484
70-71	0.0	6.723556922977622E-5	0.0	1.629016989083633	0.0027230405538059364
72-73	0.0	6.723556922977622E-5	0.0	1.7776748326506682	0.003059218399954818
74-75	0.0	6.723556922977622E-5	0.0	1.9260637339407842	0.00319368953841437
76-77	0.0	6.723556922977622E-5	0.0	2.1011787739997363	0.0032273073230292583
78-79	0.0	6.723556922977622E-5	0.0	2.2938759154122748	0.0032273073230292583
80-81	0.0	1.0085335384466432E-4	0.0	2.48771606150172	0.003361778461488811
82-83	0.0	1.3447113845955244E-4	0.0	2.688313382298757	0.003496249599948363
84-85	0.0	1.3447113845955244E-4	0.0	2.8974496203879765	0.003496249599948363
86-87	0.0	1.3447113845955244E-4	0.0	3.1292442453076297	0.003496249599948363
88-89	0.0	1.3447113845955244E-4	0.0	3.3997329403190193	0.003496249599948363
90-91	0.0	1.3447113845955244E-4	0.0	3.6874339410532317	0.003496249599948363
92-93	0.0	1.3447113845955244E-4	0.0	3.9623601836337867	0.003496249599948363
94-95	0.0	1.3447113845955244E-4	0.0	4.241824827137352	0.0035634851691781393
96-97	0.0	1.6808892307444055E-4	0.0	4.555108961963494	0.003866045230712132
98-99	0.0	2.0170670768932864E-4	0.0	4.905910544419852	0.003966898584556796
100-101	0.0	2.0170670768932864E-4	0.0	5.2817573764143	0.003966898584556796
102-103	0.0	2.0170670768932864E-4	0.0	5.670042788716257	0.00406775193840146
104-105	0.0	2.0170670768932864E-4	0.0	6.069321216587284	0.004101369723016349
106-107	0.0	2.689422769191049E-4	0.0	6.501914869011664	0.0041686052922461254
108-109	0.0	3.0256006153399297E-4	0.0	6.9709165821739685	0.004202223076861014
110-111	0.0	3.3617784614888105E-4	0.0	7.468022763274318	0.004235840861475901
112-113	0.0	3.3617784614888105E-4	0.0	7.972188679143796	0.004235840861475901
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2430	0.0	42.86554	1
GGTATCA	1645	0.0	41.611027	1
GTACATG	7640	0.0	38.954094	1
TACATGG	7900	0.0	37.590393	2
TCAACGC	2705	0.0	36.741142	4
CAACGCA	2730	0.0	36.402233	5
ACATGGG	8220	0.0	35.475433	3
CATGGGG	7140	0.0	34.256817	4
ATCAACG	3095	0.0	32.495968	3
AACGCAG	3075	0.0	32.314816	6
TATCAAC	3175	0.0	32.239487	2
ATGGGGG	4480	0.0	31.34788	5
ACGCAGA	3810	0.0	25.916834	7
GTATATA	415	0.0	24.382448	1
CGCAGAG	4110	0.0	24.315372	8
GAGTACA	5130	0.0	24.249647	1
AGTACAT	5120	0.0	23.595516	2
TGGGGGG	3615	0.0	23.537378	6
GTACCGT	285	3.1996024E-9	22.96562	6
ATGGGGA	2575	0.0	22.185448	5
>>END_MODULE