FastQCFastQC Report
Mon 15 Aug 2016
SRR2921602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487308
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.050.4149061
GTATCAA30700.044.4964331
GTACATG76200.035.5409431
TCAACGC38400.034.9396324
CAACGCA38650.034.6727335
TACATGG78300.034.3533942
ATCAACG40900.032.8094863
ACATGGG81500.032.78393
AACGCAG41300.032.7540366
TATCAAC41700.032.324162
GTATAGG4600.031.1230011
CATGGGG71300.030.44244
ATGGGGG42800.026.9968455
ACGCAGA51050.026.4974777
CGCAGAG54050.024.9148278
GCAGAGT62550.021.625119
GTATCGA1400.003129114221.2814759
AGAGTAC55300.020.95821410-11
ATGGGGA27400.020.6503855
TGGGGGG29800.020.5972866
Produced by FastQC (version 0.11.5)