##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921602_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1487308
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83937556982145	33.0	31.0	34.0	30.0	34.0
2	32.00535396837777	33.0	31.0	34.0	30.0	34.0
3	32.08232592038771	34.0	31.0	34.0	30.0	34.0
4	35.503566174591946	37.0	35.0	37.0	35.0	37.0
5	35.382948925172194	37.0	35.0	37.0	33.0	37.0
6	35.420714472052865	37.0	36.0	37.0	33.0	37.0
7	35.30895281945636	37.0	35.0	37.0	33.0	37.0
8	35.34508319729336	37.0	36.0	37.0	33.0	37.0
9	37.068368488571295	39.0	38.0	39.0	34.0	39.0
10-11	37.02662427688145	39.0	38.0	39.0	34.0	39.0
12-13	36.971951001406566	39.0	38.0	39.0	34.0	39.0
14-15	38.413599940294816	41.0	39.0	41.0	34.0	41.0
16-17	38.418059339423976	41.0	39.0	41.0	34.0	41.0
18-19	38.41833567761351	41.0	39.0	41.0	34.0	41.0
20-21	38.349290799215765	41.0	39.0	41.0	34.0	41.0
22-23	38.36325125663279	41.0	39.0	41.0	34.0	41.0
24-25	38.327224757750244	41.0	39.0	41.0	34.0	41.0
26-27	38.26171647029398	41.0	39.0	41.0	34.0	41.0
28-29	38.19562760369742	41.0	39.0	41.0	34.0	41.0
30-31	38.09382690068231	41.0	38.5	41.0	34.0	41.0
32-33	37.996176649355746	41.0	38.0	41.0	33.0	41.0
34-35	37.8760213082966	41.0	38.0	41.0	33.0	41.0
36-37	37.81189605649939	40.5	38.0	41.0	33.0	41.0
38-39	37.735203804457456	40.0	38.0	41.0	33.0	41.0
40-41	37.62079475132252	40.0	38.0	41.0	33.0	41.0
42-43	37.4779413544471	40.0	38.0	41.0	32.5	41.0
44-45	37.3469963854158	40.0	37.5	41.0	32.0	41.0
46-47	37.179056052949356	40.0	37.0	41.0	32.0	41.0
48-49	37.01718473913944	40.0	37.0	41.0	31.0	41.0
50-51	36.78052427607463	40.0	36.5	41.0	31.0	41.0
52-53	36.65513666301801	40.0	36.0	41.0	31.0	41.0
54-55	36.529729215468485	40.0	35.5	41.0	31.0	41.0
56-57	36.328093777482536	39.0	35.0	41.0	31.0	41.0
58-59	36.12901329112732	39.0	35.0	41.0	30.5	41.0
60-61	35.86654579952505	39.0	35.0	41.0	30.0	41.0
62-63	35.50766619960358	37.5	35.0	40.5	29.5	41.0
64-65	35.216452476554956	37.0	35.0	40.0	29.0	41.0
66-67	34.90820663910905	37.0	35.0	39.5	29.0	41.0
68-69	34.532525878970596	36.0	35.0	39.0	29.0	41.0
70-71	34.16006805584317	35.5	35.0	39.0	29.0	41.0
72-73	33.83763248768916	35.0	34.5	37.5	29.0	40.0
74-75	33.514953862952396	35.0	34.0	37.0	29.0	39.0
76-77	33.191722561836556	35.0	34.0	36.5	28.0	39.0
78-79	32.93352083092405	35.0	34.0	36.0	28.0	38.0
80-81	32.68724668999293	35.0	34.0	36.0	27.5	37.0
82-83	32.47326713767424	35.0	34.0	35.0	27.0	37.0
84-85	32.26759285904467	35.0	34.0	35.0	26.5	36.0
86-87	32.14784395700151	35.0	34.0	35.0	27.0	36.0
88-89	32.060236010295114	35.0	34.0	35.0	27.0	36.0
90-91	31.962540374959325	35.0	34.0	35.0	26.0	35.5
92-93	31.88556338028169	35.0	34.0	35.0	26.0	35.0
94-95	31.820722405850034	35.0	34.0	35.0	26.0	35.0
96-97	31.757652416311885	35.0	33.5	35.0	25.0	35.0
98-99	31.685938285815716	35.0	33.0	35.0	25.0	35.0
100-101	31.62290157788434	35.0	33.0	35.0	25.0	35.0
102-103	30.93006290559857	34.0	31.5	35.0	24.0	35.0
104-105	31.449528611424128	35.0	33.0	35.0	25.0	35.0
106-107	31.550661329058947	35.0	33.0	35.0	25.0	35.0
108-109	31.541127997697856	35.0	33.0	35.0	25.0	35.0
110-111	31.473931425098232	35.0	33.0	35.0	25.0	35.0
112-113	31.435174825927113	35.0	33.0	35.0	25.0	35.0
114-115	31.312816847619995	35.0	33.0	35.0	24.0	35.0
116-117	31.502282983753197	35.0	33.5	35.0	25.0	35.0
118-119	31.486522293970047	35.0	34.0	35.0	25.0	35.0
120-121	31.41193014493299	35.0	34.0	35.0	25.0	35.0
122-123	31.277983443913435	35.0	34.0	35.0	24.0	35.0
124-125	30.628555080723025	34.5	32.5	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19576.0
3	5287.0
4	2867.0
5	2377.0
6	2517.0
7	2765.0
8	3037.0
9	3394.0
10	3527.0
11	3883.0
12	4077.0
13	4900.0
14	6079.0
15	5436.0
16	3721.0
17	3352.0
18	3084.0
19	2861.0
20	2794.0
21	2982.0
22	3374.0
23	4059.0
24	5277.0
25	6686.0
26	8752.0
27	10187.0
28	13065.0
29	15278.0
30	18701.0
31	23761.0
32	30936.0
33	42052.0
34	66321.0
35	117399.0
36	273583.0
37	564172.0
38	194941.0
39	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.10290691731184	17.391737102336617	11.04378399608322	27.461571984268318
2	16.095379228177	19.030797077088167	37.66852867374355	27.205295020991283
3	19.305223668290438	22.748235047250358	27.915274197355917	30.031267087103288
4	13.546721227015185	14.351185549409735	33.57865734938266	38.523435874192415
5	14.919502353732348	37.08850033624748	32.50665770006725	15.485339609952925
6	35.92812920918178	32.99998519848163	16.115758638367957	14.956126953968624
7	31.359577870206117	31.049667116699865	18.651487968507094	18.93926704458692
8	28.70959735412402	34.75523831112181	17.88495390097875	18.65021043377541
9	28.222816773336127	15.34722054079939	17.795170415567014	38.63479227029746
10-11	27.55198044197293	24.630789428628034	25.733114870780927	22.08411525861811
12-13	27.21705389331125	21.684232407294665	26.40801178060573	24.690701918788356
14-15	24.46605226981393	23.187578056755523	23.600595627913513	28.74577404551703
16-17	25.033784851874696	26.405829896550078	24.2579272480464	24.302458003528827
18-19	24.99933581988915	25.642766607781635	25.75653477562987	23.601362796699345
20-21	25.50363756925101	24.666741090342718	25.736592642009114	24.093028698397156
22-23	25.464675641475615	24.12480820615435	25.632902881378953	24.77761327099109
24-25	24.94755406973695	24.61657318291661	25.59078995673883	24.845082790607606
26-27	24.683689630398874	24.84065555208535	25.750950317882047	24.724704499633724
28-29	24.80563080644977	25.276265475741422	25.451925604825558	24.46617811298325
30-31	25.335907161974035	24.79740680713128	25.451924437823852	24.414761593070832
32-33	24.94489593097299	24.74102096173579	25.539172638795687	24.774910468495534
34-35	24.93855251200962	24.95297514768073	25.689168331657413	24.419304008652237
36-37	24.93049945250143	24.965195094063176	25.656216621834655	24.448088831600735
38-39	25.014254228194055	25.089458729302795	25.560587865886543	24.33569917661661
40-41	25.038972478051193	24.81463489251261	25.59159666973157	24.554795959704627
42-43	24.890587609588888	24.728746697793195	25.542288082202003	24.83837761041591
44-45	24.7156485418667	24.87544074812203	25.605280639895	24.80363007011627
46-47	24.694511169122826	24.90049571439858	25.51606239042258	24.888930726056017
48-49	24.644307901052695	25.13049859928534	25.409464884303624	24.81572861535834
50-51	24.75075439323117	25.160896880765527	25.47210089774139	24.616247828261912
52-53	24.911450159954494	25.078061020723485	25.273986786777098	24.736502032544923
54-55	24.581970776196144	24.777264562397693	25.56697897759383	25.073785683812332
56-57	24.656886755568223	25.013573248983644	25.296670040788666	25.032869954659464
58-59	24.695530389138163	25.079548537466728	25.445782776098557	24.779138297296544
60-61	25.038752992410096	24.95470833309521	25.507587719932268	24.49895095456243
62-63	24.88261395615593	24.999100715494286	25.513356980313567	24.604928348036214
64-65	24.730292827811333	24.70228920381293	25.517386283603088	25.05003168477265
66-67	24.645041780060883	25.02577739825618	25.3458642698533	24.98331655182963
68-69	24.516833059178182	24.98183785143043	25.513675316253348	24.98765377313804
70-71	24.497287884314442	24.88012734421093	25.6476216249834	24.974963146491227
72-73	24.662300725490326	25.160797972123422	25.2651838603616	24.91171744202465
74-75	24.479375913401395	25.078274738364186	25.401997472481554	25.04035187575287
76-77	24.53430943255156	24.781572810239897	25.392755435171775	25.291362322036765
78-79	24.42262198317696	24.868260085100204	25.51234430317107	25.196773628551767
80-81	24.583139470775386	25.091406756367878	25.25862161704233	25.0668321558144
82-83	24.61208938358916	25.11238170545857	25.314065304604767	24.961463606347504
84-85	24.523291480701037	25.16984001688987	25.097795845038128	25.209072657370964
86-87	24.249987225241846	25.2687069524268	25.297551011626375	25.18375481070498
88-89	24.17260309893531	25.01248920011968	25.37741589540673	25.437491805538283
90-91	24.44801840650225	24.867133440753904	25.393624220525712	25.291223932218138
92-93	24.464132513238685	25.012606669230582	25.396051120547998	25.12720969698274
94-95	24.37253994914158	24.860986535024672	25.389103226995037	25.377370288838712
96-97	24.103335596958225	24.843339227722904	25.481614211081897	25.571710964236967
98-99	24.03649843860992	24.915744775839922	25.33657545754999	25.711181328000166
100-101	24.061996573675394	24.955792613231424	25.391277395132683	25.590933417960503
102-103	23.87791457799153	24.861148137900802	25.579234397676576	25.681702886431097
104-105	24.106909933920882	24.890540493293926	25.39319360885573	25.609355963929463
106-107	24.02026355803686	24.721668553194192	25.50994234184686	25.74812554692209
108-109	24.044981593854533	24.701349784000943	25.46724714662722	25.786421475517308
110-111	23.865643116840328	25.05957913881292	25.42076873813922	25.654009006207527
112-113	23.776157245699096	25.065168294557328	25.468045179764985	25.690629279978594
114-115	23.81967958217128	25.09745795759856	25.374367649471395	25.708494810758765
116-117	23.60233767927777	24.976551264147403	25.473193808592427	25.9479172479824
118-119	23.66307508698795	24.85391067556437	25.46721352809669	26.015800709350994
120-121	23.51871512492999	24.917718535870684	25.616312539795988	25.947253799403335
122-123	23.41815749087688	25.03384505737775	25.434741316855963	26.113256134889408
124-125	23.32571577406842	24.94488739057821	25.44676463491745	26.28263220043592
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	6.5
2	9.0
3	22.5
4	26.5
5	24.5
6	73.0
7	193.0
8	352.0
9	417.5
10	362.5
11	306.0
12	296.5
13	333.5
14	355.5
15	371.5
16	380.0
17	431.0
18	494.5
19	502.5
20	507.5
21	545.5
22	609.5
23	694.5
24	825.0
25	975.5
26	1267.5
27	1748.5
28	2328.0
29	3155.5
30	4386.5
31	5981.5
32	7905.0
33	9906.5
34	12103.0
35	14537.5
36	17497.0
37	20733.5
38	24016.5
39	27445.0
40	30604.5
41	33587.5
42	36817.0
43	41236.5
44	45896.5
45	50559.5
46	54844.0
47	58469.0
48	63047.0
49	66806.5
50	69026.5
51	70560.5
52	71031.5
53	70726.5
54	70206.5
55	68195.5
56	64249.5
57	59167.5
58	53917.0
59	48515.5
60	41803.5
61	35395.0
62	29258.0
63	22498.5
64	16550.0
65	12380.0
66	9428.5
67	7331.5
68	5836.0
69	4466.5
70	3394.0
71	2552.5
72	1849.5
73	1464.0
74	1162.0
75	850.0
76	612.5
77	389.0
78	219.0
79	116.0
80	68.0
81	34.5
82	20.0
83	9.5
84	6.5
85	5.5
86	5.5
87	3.0
88	1.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025011631753476753
2	0.0016136536615146294
3	0.029650886030331308
4	0.16600462042831746
5	0.020708555322771074
6	0.06548744442980203
7	0.05049391249156193
8	0.04148434621477192
9	0.031735188676454376
10-11	0.057788971752992656
12-13	0.05358674867613165
14-15	0.05442719329150385
16-17	0.04672872061469446
18-19	0.03445822923026031
20-21	0.021918795568907044
22-23	0.002286009353812391
24-25	0.0041686052922461254
26-27	0.002857511692265489
28-29	0.00410136972301635
30-31	0.0019834492922783985
32-33	0.0082027394460327
34-35	0.004202223076861013
36-37	0.006017583446064971
38-39	0.0020170670768932864
40-41	0.002723040553805937
42-43	0.0030256006153399294
44-45	0.004303076430705678
46-47	0.00406775193840146
48-49	0.0021179204307379506
50-51	0.0013447113845955242
52-53	6.723556922977621E-4
54-55	0.004134987507631237
56-57	5.042667692233216E-4
58-59	5.715023384530978E-4
60-61	2.3532449230421676E-4
62-63	9.749157538317552E-4
64-65	3.361778461488811E-5
66-67	0.00373157409225258
68-69	7.732090461424265E-4
70-71	3.361778461488811E-5
72-73	0.0025549516307314963
74-75	0.005277992184537433
76-77	0.001848978153818846
78-79	1.6808892307444053E-4
80-81	0.0
82-83	0.005210756615307656
84-85	0.0017481247999741817
86-87	0.0
88-89	0.001546418092284853
90-91	5.042667692233216E-4
92-93	0.0
94-95	0.002823893907650601
96-97	0.0012102402461359719
98-99	7.732090461424265E-4
100-101	0.0
102-103	0.0014455647384401886
104-105	0.0
106-107	5.042667692233216E-4
108-109	0.0022187737845826153
110-111	3.361778461488811E-5
112-113	3.3617784614888105E-4
114-115	0.0
116-117	0.0014119469538253005
118-119	0.0022187737845826153
120-121	0.0023532449230421674
122-123	0.0010421513230615314
124-125	0.09994567366006235
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1487308.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.15120637619751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.17768826297231	49.83140049998421
2	10.912684199082525	13.564729755508953
3	3.4903110257563728	6.5078112263670596
4	1.683129034227832	4.184339998562559
5	0.9832950769715881	3.05564876287801
6	0.6011539255615748	2.241746501486322
7	0.41912368622218393	1.8234329903683324
8	0.3083749328684327	1.5332699275161612
9	0.22442781362054143	1.2553613424800154
>10	1.1353181260218084	12.300058297080113
>50	0.050821948139167294	2.096053990802282
>100	0.01312946564593153	1.2919697413746998
>500	3.255017458321596E-4	0.14255636515563794
>1k	2.1700116388810643E-4	0.1716206004357136
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	1.3447113845955244E-4
10-11	0.0	0.0	0.0	0.0	1.3447113845955244E-4
12-13	0.0	0.0	0.0	0.0	2.0170670768932864E-4
14-15	0.0	0.0	0.0	0.0	3.0256006153399297E-4
16-17	0.0	0.0	0.0	6.723556922977622E-5	4.034134153786573E-4
18-19	0.0	0.0	0.0	1.3447113845955244E-4	4.7064898460843347E-4
20-21	0.0	6.723556922977622E-5	0.0	2.3532449230421676E-4	6.387379076828741E-4
22-23	0.0	6.723556922977622E-5	0.0	3.6979563076376914E-4	8.404446153722027E-4
24-25	0.0	6.723556922977622E-5	0.0	8.740623999870908E-4	9.412979692168669E-4
26-27	0.0	6.723556922977622E-5	0.0	0.0013447113845955244	0.0010085335384466432
28-29	0.0	6.723556922977622E-5	0.0	0.0030928361845697057	0.0010085335384466432
30-31	0.0	6.723556922977622E-5	0.0	0.010085335384466432	0.0010085335384466432
32-33	0.0	6.723556922977622E-5	0.0	0.027264023322674254	0.0010421513230615314
34-35	0.0	6.723556922977622E-5	0.0	0.04676233839930936	0.0012102402461359719
36-37	0.0	1.0085335384466432E-4	0.0	0.07876646935268283	0.0012102402461359719
38-39	0.0	1.3447113845955244E-4	0.0	0.1311429777826785	0.0012102402461359719
40-41	0.0	1.3447113845955244E-4	0.0	0.19353758602791082	0.0012102402461359719
42-43	0.0	1.3447113845955244E-4	0.0	0.25095676215013973	0.00124385803075086
44-45	0.0	1.3447113845955244E-4	0.0	0.3148305529184271	0.001311093599980636
46-47	0.0	1.3447113845955244E-4	0.0	0.3797464950097761	0.0013447113845955242
48-49	0.0	1.3447113845955244E-4	0.0	0.451554082947177	0.0014791825230550768
50-51	0.0	1.3447113845955244E-4	0.0	0.5252778846076267	0.0014791825230550768
52-53	0.0	1.3447113845955244E-4	0.0	0.6004136332219017	0.0015464180922848529
54-55	0.0	1.6808892307444055E-4	0.0	0.6855674816514131	0.0017145070153592934
56-57	0.0	2.0170670768932864E-4	0.0	0.7788568339577276	0.0017481247999741815
58-59	0.0	2.0170670768932864E-4	0.0	0.8839796464484828	0.0017481247999741815
60-61	0.0	2.0170670768932864E-4	0.0	0.9955234558006816	0.0017481247999741815
62-63	0.0	2.0170670768932864E-4	0.0	1.1114711949374305	0.0018825959384337339
64-65	3.361778461488811E-5	2.0170670768932864E-4	0.0	1.2250993069357525	0.0019834492922783985
66-67	6.723556922977622E-5	2.0170670768932864E-4	0.0	1.3557044001645928	0.0020170670768932864
68-69	6.723556922977622E-5	2.0170670768932864E-4	0.0	1.5032528568393366	0.0020170670768932864
70-71	6.723556922977622E-5	2.0170670768932864E-4	0.0	1.6643156629292655	0.0020170670768932864
72-73	6.723556922977622E-5	2.0170670768932864E-4	0.0	1.8169740228654723	0.0020170670768932864
74-75	6.723556922977622E-5	2.0170670768932864E-4	0.0	1.9694642938786049	0.0020506848615081744
76-77	6.723556922977622E-5	2.0170670768932864E-4	0.0	2.149050499291337	0.0020843026461230627
78-79	6.723556922977622E-5	2.0170670768932864E-4	0.0	2.347361810734562	0.002151538215352839
80-81	6.723556922977622E-5	2.0170670768932864E-4	0.0	2.5462110067316255	0.0022187737845826153
82-83	6.723556922977622E-5	2.0170670768932864E-4	0.0	2.752624204267038	0.0025549516307314963
84-85	6.723556922977622E-5	2.0170670768932864E-4	0.0	2.967643554663862	0.0025549516307314963
86-87	6.723556922977622E-5	2.0170670768932864E-4	0.0	3.2074055945372444	0.002723040553805937
88-89	6.723556922977622E-5	2.0170670768932864E-4	0.0	3.485727233363903	0.0028911294768803773
90-91	6.723556922977622E-5	2.3532449230421676E-4	0.0	3.780925000067236	0.0028911294768803773
92-93	6.723556922977622E-5	2.689422769191049E-4	0.0	4.063650568678445	0.0030928361845697057
94-95	6.723556922977622E-5	2.689422769191049E-4	0.0	4.351822218397265	0.0032273073230292583
96-97	6.723556922977622E-5	2.689422769191049E-4	0.0	4.674653804054036	0.0033281606768739225
98-99	6.723556922977622E-5	2.689422769191049E-4	0.0	5.035742428602549	0.0034290140307185867
100-101	6.723556922977622E-5	2.689422769191049E-4	0.0	5.420027324535335	0.0034290140307185867
102-103	6.723556922977622E-5	2.689422769191049E-4	0.0	5.815439707175649	0.003529867384563251
104-105	6.723556922977622E-5	2.689422769191049E-4	0.0	6.224668999292682	0.0035634851691781393
106-107	6.723556922977622E-5	2.689422769191049E-4	0.0	6.665734333440014	0.0035634851691781393
108-109	6.723556922977622E-5	2.689422769191049E-4	0.0	7.147712511463665	0.0035971029537930277
110-111	6.723556922977622E-5	2.689422769191049E-4	0.0	7.65688747724076	0.0037651918768674677
112-113	6.723556922977622E-5	2.689422769191049E-4	0.0	8.176450338463855	0.0038996630153270203
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1905	0.0	50.414906	1
GTATCAA	3070	0.0	44.496433	1
GTACATG	7620	0.0	35.540943	1
TCAACGC	3840	0.0	34.939632	4
CAACGCA	3865	0.0	34.672733	5
TACATGG	7830	0.0	34.353394	2
ATCAACG	4090	0.0	32.809486	3
ACATGGG	8150	0.0	32.7839	3
AACGCAG	4130	0.0	32.754036	6
TATCAAC	4170	0.0	32.32416	2
GTATAGG	460	0.0	31.123001	1
CATGGGG	7130	0.0	30.4424	4
ATGGGGG	4280	0.0	26.996845	5
ACGCAGA	5105	0.0	26.497477	7
CGCAGAG	5405	0.0	24.914827	8
GCAGAGT	6255	0.0	21.62511	9
GTATCGA	140	0.0031291142	21.281475	9
AGAGTAC	5530	0.0	20.958214	10-11
ATGGGGA	2740	0.0	20.650385	5
TGGGGGG	2980	0.0	20.597286	6
>>END_MODULE