FastQCFastQC Report
Mon 15 Aug 2016
SRR2921601_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921601_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128275
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38300.045.533811
GGTATCA24150.042.391271
GTACATG111500.037.1534771
TCAACGC47600.036.0038154
CAACGCA49050.035.059155
TACATGG117050.034.519252
TATCAAC50850.034.0537532
AACGCAG53400.032.868696
ACATGGG121300.032.7701643
CATGGGG105100.032.7256364
ATCAACG52250.032.685763
ATGGGGG62350.029.3938715
ACGCAGA62850.027.6335267
CGCAGAG67050.025.549548
ATGGGGA42600.023.4661715
GTATAAT7250.022.1661821
GCAGAGT77800.022.0192369
AGAGTAC66400.021.05204410-11
GTATATA6050.019.6761321
GAGTACA79250.019.1516421
Produced by FastQC (version 0.11.5)