##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921601_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2128275
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.027298633870153	31.0	31.0	34.0	26.0	34.0
2	30.11252493216337	31.0	31.0	34.0	26.0	34.0
3	30.28784109196415	31.0	31.0	34.0	26.0	34.0
4	33.841999271710655	37.0	35.0	37.0	30.0	37.0
5	33.79047585485898	37.0	35.0	37.0	30.0	37.0
6	33.77454699228248	37.0	35.0	37.0	29.0	37.0
7	33.58050956760757	37.0	35.0	37.0	28.0	37.0
8	33.60971068118547	37.0	35.0	37.0	28.0	37.0
9	35.26162455509744	39.0	37.0	39.0	29.0	39.0
10-11	35.34383244646486	39.0	37.0	39.0	29.5	39.0
12-13	35.32824047644219	39.0	37.0	39.0	29.0	39.0
14-15	36.605837591476664	40.0	38.0	41.0	29.0	41.0
16-17	36.56024573892002	40.0	38.0	41.0	28.5	41.0
18-19	36.49653404752675	40.0	38.0	41.0	28.0	41.0
20-21	36.55875720947716	40.0	38.0	41.0	29.0	41.0
22-23	36.45932269091165	40.0	38.0	41.0	28.0	41.0
24-25	36.43015446782019	40.0	38.0	41.0	28.0	41.0
26-27	36.391855375832534	40.0	38.0	41.0	28.0	41.0
28-29	36.33588258096346	40.0	38.0	41.0	28.0	41.0
30-31	36.24764562850196	40.0	37.5	41.0	27.5	41.0
32-33	36.17013990203334	40.0	37.0	41.0	27.0	41.0
34-35	36.031752945460525	40.0	37.0	41.0	27.0	41.0
36-37	35.92128578308724	40.0	37.0	41.0	26.0	41.0
38-39	35.7985229822274	40.0	37.0	41.0	26.0	41.0
40-41	35.6888879491607	40.0	36.0	41.0	25.5	41.0
42-43	35.4170823789219	40.0	35.5	41.0	24.5	41.0
44-45	35.00572834807532	39.5	35.0	41.0	23.5	41.0
46-47	34.72477499383304	39.0	35.0	41.0	23.0	41.0
48-49	34.5121734738227	39.0	35.0	40.5	22.0	41.0
50-51	34.29632730732635	38.5	34.0	40.0	21.0	41.0
52-53	34.074760075648115	38.0	34.0	40.0	20.0	41.0
54-55	33.82134310650645	38.0	34.0	40.0	18.5	41.0
56-57	33.55676897957266	38.0	33.0	40.0	18.0	41.0
58-59	33.56437584428704	37.5	34.0	40.0	18.0	41.0
60-61	33.34650244916658	37.0	33.0	40.0	17.0	41.0
62-63	33.04185948714334	36.5	33.0	40.0	13.5	41.0
64-65	32.739060976612514	36.0	33.0	39.0	13.0	41.0
66-67	32.417164840070015	35.5	33.0	39.0	9.5	41.0
68-69	32.085687939763424	35.0	32.5	38.5	8.0	40.0
70-71	31.722514477687326	35.0	32.0	37.5	7.0	40.0
72-73	31.38647026347629	35.0	32.0	37.0	6.5	39.0
74-75	31.025442200843408	35.0	31.5	36.5	2.0	39.0
76-77	30.740659689184902	35.0	31.0	36.0	2.0	38.5
78-79	30.48226192573798	35.0	31.0	35.5	2.0	37.0
80-81	30.2318807484935	35.0	31.0	35.0	2.0	37.0
82-83	30.016524650244918	35.0	31.0	35.0	2.0	36.5
84-85	29.814576123856174	34.5	31.0	35.0	2.0	36.0
86-87	29.659584405210794	34.0	31.0	35.0	2.0	36.0
88-89	29.504921591429678	34.0	31.0	35.0	2.0	35.0
90-91	29.32769919300842	34.0	30.0	35.0	2.0	35.0
92-93	29.258033853707815	34.0	30.0	35.0	2.0	35.0
94-95	29.142466551549965	34.0	30.0	35.0	2.0	35.0
96-97	29.06200420528362	34.0	30.0	35.0	2.0	35.0
98-99	28.975586096721525	34.0	30.0	35.0	2.0	35.0
100-101	28.8686903713101	34.0	29.5	35.0	2.0	35.0
102-103	27.963713570849634	32.5	28.0	34.5	2.0	35.0
104-105	28.930576170842585	34.0	30.0	35.0	2.0	35.0
106-107	29.236706252716402	34.0	31.0	35.0	2.0	35.0
108-109	29.246283492499796	34.0	31.0	35.0	2.0	35.0
110-111	29.19727901704432	34.0	31.0	35.0	2.0	35.0
112-113	29.122143285054797	34.0	31.0	35.0	2.0	35.0
114-115	29.012065170149533	34.0	31.0	35.0	2.0	35.0
116-117	28.898469417720925	34.0	30.5	35.0	2.0	35.0
118-119	28.620828840257957	34.0	30.0	35.0	2.0	35.0
120-121	28.501664258613197	34.0	30.0	35.0	2.0	35.0
122-123	28.255260480905896	34.0	30.0	35.0	2.0	35.0
124-125	27.44797312377395	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91512.0
3	16260.0
4	7548.0
5	6736.0
6	6982.0
7	7627.0
8	7989.0
9	8445.0
10	8778.0
11	9015.0
12	8870.0
13	8534.0
14	8282.0
15	7718.0
16	7164.0
17	6504.0
18	6131.0
19	5917.0
20	5925.0
21	6188.0
22	6986.0
23	9138.0
24	12106.0
25	15514.0
26	18179.0
27	20980.0
28	24988.0
29	30022.0
30	37216.0
31	47120.0
32	62302.0
33	87215.0
34	135442.0
35	225904.0
36	436667.0
37	585925.0
38	130405.0
39	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.749366607937006	17.525844417009072	12.690634444779162	25.034154530274755
2	16.40414274033627	18.94096031126942	38.75177391614898	25.90312303224533
3	19.650558578099904	23.171522525917705	28.427757952936023	28.750160943046367
4	13.723993846020823	15.007325941916887	34.403027366786745	36.86565284527554
5	15.060471402496677	37.48677129328348	32.4198241686357	15.03293313558414
6	35.38562323262583	33.279141304812995	16.634350535109053	14.700884927452126
7	31.364268460894035	30.774503342080695	19.318157243744825	18.543070953280445
8	27.466024848516184	34.97816470720964	18.693831118465276	18.861979325808893
9	27.92090785272302	15.844654390138674	18.227363604490385	38.00707415264792
10-11	26.86716434995028	25.08115499940886	26.116079560692373	21.935601089948488
12-13	26.699045778748108	22.469664260358698	26.486077655358176	24.34521230553501
14-15	24.330293149199004	23.784568660755493	23.61896901944897	28.266169170596534
16-17	24.975858591627016	26.691338022724064	24.209772799324675	24.123030586324248
18-19	25.011134449387086	26.12079543801337	25.267855485070672	23.60021462752887
20-21	25.670195735976858	25.06728042330032	25.166802809844796	24.095721030878025
22-23	25.937253674350263	24.5467843060391	24.885488684237366	24.630473335373267
24-25	25.299935796866173	25.109159427755497	24.857775488315124	24.73312928706321
26-27	24.88051401244229	25.259202624496346	25.103961916786748	24.756321446274608
28-29	25.050779114494976	25.281911635644516	25.126107539376434	24.54120171048407
30-31	25.39211288245179	25.105000128070433	24.875530816682804	24.627356172794972
32-33	24.966800748325586	25.27163932041584	24.89092010160346	24.87063982965512
34-35	25.005834610131707	25.551314837913004	24.97297747029637	24.469873081658925
36-37	25.302351284332147	25.289640242266355	24.85342359347973	24.55458487992177
38-39	25.246439779073032	25.376993941074392	24.84124252033447	24.535323759518104
40-41	25.131438735330214	25.27386497334561	24.91009049941784	24.684605791906336
42-43	25.14974309278409	25.143586530946592	24.918026084083596	24.788644292185722
44-45	25.0230738721059	25.10378543084699	25.060339820333954	24.812800876713155
46-47	24.998308114845162	25.144985158407447	25.1001502018043	24.75655652494309
48-49	24.964409407666324	25.272845541294252	24.954421951910753	24.80832309912867
50-51	24.894169014680276	25.329148816671644	25.023346971003697	24.75333519764439
52-53	25.079426624368462	25.17758195276701	25.00730818940195	24.735683233462577
54-55	24.93650665340716	25.000399477014092	25.06088499667729	25.002208872901456
56-57	24.928233173099517	25.14531959972252	24.928632661003025	24.997814566174938
58-59	24.929361721325325	25.164074657934503	25.02050712938489	24.886056491355284
60-61	25.06726427894338	25.074102324418497	25.15265760284914	24.705975793788987
62-63	24.95786101996893	25.202599610251152	25.05058674990207	24.78895261987785
64-65	24.879560227405705	25.03126428981303	25.095625725211683	24.99354975756958
66-67	24.943195183675368	25.21057555398943	24.890179705082602	24.956049557252605
68-69	24.87105244235486	25.112005199729488	24.984338305754807	25.032604052160845
70-71	24.802816855063394	24.981185397518118	25.22428275350826	24.99171499391023
72-73	24.93670784944857	25.190252421362413	24.900826240429808	24.97221348875921
74-75	24.832207826871088	25.22977295485741	24.955135594120332	24.98288362415117
76-77	24.888871240079006	24.960959840446403	24.965588736884598	25.184580182589993
78-79	24.843726244050952	25.00377745291813	24.99548233080242	25.15701397222849
80-81	24.93253294753654	25.230151645491155	24.886054992021442	24.951260414950863
82-83	24.916304221904785	25.180403849900053	24.995235679368623	24.908056248826544
84-85	24.88815936632413	25.189353711511313	24.85526506230181	25.067221859862748
86-87	24.717927582497577	25.197364927386527	25.05396137338451	25.030746116731383
88-89	24.756285949672925	25.051825805138733	24.988192034875443	25.2036962103129
90-91	24.874416020986352	24.978111801580837	25.028796550899283	25.118675626533527
92-93	24.872380981917537	25.042931256186527	25.050026749068365	25.03466101282757
94-95	24.858127992011514	24.927721812681725	25.124473552820938	25.08967664248583
96-97	24.717942138928695	24.905867146559185	25.171471583450288	25.204719131061832
98-99	24.65423599234922	25.00656086426259	25.008111507203797	25.331091636184393
100-101	24.617383530440033	25.092272250423207	25.12121856464344	25.169125654493318
102-103	24.56987419564695	24.925341329503468	25.261002859008297	25.243781615841286
104-105	24.462036862788768	25.04511463852256	25.162021521109846	25.33082697757883
106-107	24.476251290371536	24.896875996998492	25.282613098099247	25.344259614530724
108-109	24.451424947620772	24.965595476107623	25.26609023865473	25.31688933761687
110-111	24.410893579191676	25.149554010359243	25.18324374854487	25.256308661904214
112-113	24.353950607161327	25.217017855502487	25.23578938020372	25.193242157132463
114-115	24.299931485155586	25.279839436542943	25.166611372336355	25.253617705965116
116-117	24.22857105266002	25.17784133813184	25.23326477649012	25.360322832718012
118-119	24.283012969782718	25.073044397189083	25.198697485831044	25.445245147197156
120-121	24.181601999945027	25.165270102292553	25.342057837429056	25.311070060333368
122-123	24.184285927739964	25.15355281173617	25.2828134558874	25.37934780463647
124-125	24.088842093455707	25.208617638762455	25.277946727829598	25.424593539952244
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	488.0
1	395.5
2	277.0
3	199.0
4	132.5
5	125.0
6	134.5
7	152.5
8	176.0
9	190.5
10	197.0
11	197.5
12	210.5
13	231.0
14	270.0
15	305.5
16	338.0
17	350.5
18	385.0
19	461.0
20	511.0
21	599.0
22	706.5
23	821.5
24	982.5
25	1216.5
26	1597.0
27	2144.5
28	2820.5
29	3879.5
30	5568.0
31	7823.0
32	10770.0
33	13978.5
34	17454.5
35	21509.5
36	26485.0
37	31521.5
38	36168.5
39	41180.0
40	45909.0
41	50318.0
42	55076.0
43	61304.5
44	67551.0
45	73669.5
46	79831.5
47	84777.0
48	90066.0
49	94565.0
50	96400.0
51	97461.0
52	97143.5
53	96310.0
54	96474.0
55	94470.5
56	89238.5
57	82145.5
58	75759.0
59	68920.0
60	59778.0
61	50951.5
62	42844.5
63	33512.5
64	24977.5
65	19152.5
66	14791.0
67	11669.5
68	9259.0
69	7172.5
70	5715.0
71	4447.0
72	3406.0
73	2698.0
74	2115.0
75	1644.0
76	1164.5
77	770.5
78	532.5
79	360.0
80	249.0
81	181.0
82	135.5
83	118.0
84	95.0
85	69.0
86	51.5
87	42.0
88	40.5
89	38.5
90	36.5
91	30.5
92	23.0
93	19.5
94	17.0
95	15.5
96	15.0
97	11.5
98	8.0
99	8.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.020955938494790383
2	0.010102077973946036
3	0.00906837697196086
4	0.010383996429032902
5	0.015364555802234203
6	0.08861636771563826
7	0.05436327542258401
8	0.04628161304342719
9	0.024855810456825365
10-11	0.047855657750995526
12-13	0.012075507159554098
14-15	0.025983484277172827
16-17	0.033195898086478486
18-19	0.04080769637382387
20-21	0.02880266882804149
22-23	0.03552172534094513
24-25	0.03068212519528726
26-27	0.043861812970598255
28-29	0.04341544208337738
30-31	0.02534916775322738
32-33	0.027792461030646885
34-35	0.04151249251154104
36-37	0.009585227472953449
38-39	0.01985175787903349
40-41	0.04031433907742186
42-43	0.021801693860050982
44-45	0.015364555802234203
46-47	0.02213059872431899
48-49	0.028473763963773477
50-51	0.027721981416875166
52-53	0.024667864820100787
54-55	0.023164299726304165
56-57	0.025889511458810535
58-59	0.01675065487307796
60-61	0.022271557951862423
62-63	0.02323477934007588
64-65	0.021049911313152668
66-67	0.02793342025819032
68-69	0.022177585133500134
70-71	0.0440732518119134
72-73	0.020861965676428094
74-75	0.009209336199504294
76-77	0.01588140630322679
78-79	0.024362453160423347
80-81	0.018066274330150005
82-83	0.022529983202358717
84-85	0.011605643067742656
86-87	0.017103052941936543
88-89	0.022271557951862423
90-91	0.019546346219356053
92-93	0.0076822779011171015
94-95	0.009984611950993176
96-97	0.01402544314057159
98-99	0.005896794352233616
100-101	0.009279815813276011
102-103	0.004205283621712419
104-105	0.004017337984987842
106-107	5.638369101737322E-4
108-109	0.013367633412035567
110-111	0.0014330854800249028
112-113	0.002419800072828934
114-115	0.012639344069727831
116-117	0.005356450646650456
118-119	0.27085315572470664
120-121	7.752757514888818E-4
122-123	7.98768956079454E-4
124-125	0.3059050169738403
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2128275.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.797089664669755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.41339429066636	50.311108268854234
2	9.91910628992088	12.259438015832606
3	3.2632856884948716	6.049846748800534
4	1.5432235633181899	3.8146689966002145
5	0.9530371752074132	2.944746188502805
6	0.5929811113338566	2.1986704143932316
7	0.4243025608476717	1.8354464378356794
8	0.29952186941609027	1.4807663856668511
9	0.22804358530719004	1.268318689981307
>10	1.2886223655152953	13.974423436931122
>50	0.06034623570787904	2.5115178281208244
>100	0.013983483977113105	1.2919572343868395
>500	1.5178028715869893E-4	0.05909135409371528
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.698640918114435E-5
7	0.0	0.0	0.0	0.0	4.698640918114435E-5
8	0.0	0.0	0.0	0.0	9.39728183622887E-5
9	0.0	0.0	0.0	0.0	9.39728183622887E-5
10-11	0.0	0.0	0.0	0.0	9.39728183622887E-5
12-13	0.0	0.0	0.0	0.0	9.39728183622887E-5
14-15	0.0	0.0	0.0	0.0	1.1746602295286087E-4
16-17	0.0	0.0	0.0	0.0	2.3493204590572178E-4
18-19	0.0	0.0	0.0	4.698640918114435E-5	2.5842525049629396E-4
20-21	0.0	0.0	0.0	7.047961377171653E-5	3.289048642680105E-4
22-23	0.0	0.0	0.0	2.114388413151496E-4	3.5239806885858265E-4
24-25	0.0	0.0	0.0	2.819184550868661E-4	3.758912734491548E-4
26-27	0.0	0.0	0.0	4.228776826302992E-4	3.758912734491548E-4
28-29	0.0	0.0	0.0	0.0016445243213400523	3.758912734491548E-4
30-31	0.0	2.3493204590572176E-5	0.0	0.007729264310298247	3.758912734491548E-4
32-33	0.0	4.698640918114435E-5	0.0	0.020556554016750654	3.758912734491548E-4
34-35	0.0	4.698640918114435E-5	0.0	0.038317416687223216	4.228776826302992E-4
36-37	0.0	4.698640918114435E-5	0.0	0.06310274753027686	4.228776826302992E-4
38-39	0.0	7.047961377171653E-5	0.0	0.10273578367457213	4.228776826302992E-4
40-41	0.0	9.39728183622887E-5	0.0	0.1467620490773044	4.228776826302992E-4
42-43	0.0	9.39728183622887E-5	0.0	0.1913286581856198	4.228776826302992E-4
44-45	0.0	9.39728183622887E-5	0.0	0.24797077445348933	4.228776826302992E-4
46-47	0.0	9.39728183622887E-5	0.0	0.31058016468736416	4.228776826302992E-4
48-49	0.0	9.39728183622887E-5	0.0	0.3711926325310404	4.6986409181144355E-4
50-51	0.0	9.39728183622887E-5	0.0	0.4328153081721112	4.6986409181144355E-4
52-53	0.0	9.39728183622887E-5	0.0	0.49930107716343053	4.933572964020157E-4
54-55	0.0	9.39728183622887E-5	0.0	0.5755130328552467	5.4034370558316E-4
56-57	0.0	9.39728183622887E-5	0.0	0.6632366587964431	5.638369101737322E-4
58-59	0.0	9.39728183622887E-5	0.0	0.7508193255100961	5.638369101737322E-4
60-61	0.0	9.39728183622887E-5	0.0	0.8417145340710199	5.638369101737322E-4
62-63	0.0	9.39728183622887E-5	0.0	0.9386005098025396	6.108233193548766E-4
64-65	0.0	1.4095922754343306E-4	0.0	1.0428163653663178	6.108233193548766E-4
66-67	0.0	1.4095922754343306E-4	0.0	1.1578860814509402	6.108233193548766E-4
68-69	0.0	1.4095922754343306E-4	0.0	1.2889311766571518	6.108233193548766E-4
70-71	0.0	1.6445243213400522E-4	0.0	1.4262959438982274	6.813029331265931E-4
72-73	0.0	1.879456367245774E-4	0.0	1.5600192644277642	7.517825468983096E-4
74-75	0.0	1.879456367245774E-4	0.0	1.7031163735889394	7.517825468983096E-4
76-77	0.0	1.879456367245774E-4	0.0	1.8703644970692226	7.517825468983096E-4
78-79	0.0	1.879456367245774E-4	0.0	2.0525073122599284	7.517825468983096E-4
80-81	0.0	1.879456367245774E-4	0.0	2.241110758713042	7.752757514888818E-4
82-83	0.0	1.879456367245774E-4	0.0	2.44714616297236	7.98768956079454E-4
84-85	0.0	1.879456367245774E-4	0.0	2.662625835477088	7.98768956079454E-4
86-87	0.0	1.879456367245774E-4	0.0	2.896124795902785	7.98768956079454E-4
88-89	0.0	1.879456367245774E-4	0.0	3.1635949301664494	7.98768956079454E-4
90-91	0.0	1.879456367245774E-4	0.0	3.4442447522054245	7.98768956079454E-4
92-93	0.0	2.5842525049629396E-4	0.0	3.732083494849115	7.98768956079454E-4
94-95	0.0	2.819184550868661E-4	0.0	4.037166249662285	8.692485698511706E-4
96-97	0.0	2.819184550868661E-4	0.0	4.355734103910445	9.867145928040314E-4
98-99	0.0	2.819184550868661E-4	0.0	4.718187264333792	9.867145928040314E-4
100-101	0.0	2.819184550868661E-4	0.0	5.112285771340639	0.0010337010019851758
102-103	0.0	2.819184550868661E-4	0.0	5.522524109901211	0.0010337010019851758
104-105	0.0	2.819184550868661E-4	0.0	5.943428363345902	0.0010337010019851758
106-107	0.0	2.819184550868661E-4	0.0	6.393017819595682	0.0010571942065757478
108-109	0.0	2.819184550868661E-4	0.0	6.868120896030823	0.00108068741116632
110-111	0.0	2.819184550868661E-4	0.0	7.38083659301547	0.001174660229528609
112-113	0.0	2.819184550868661E-4	0.0	7.905792249591806	0.0012686330478908975
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3830	0.0	45.53381	1
GGTATCA	2415	0.0	42.39127	1
GTACATG	11150	0.0	37.153477	1
TCAACGC	4760	0.0	36.003815	4
CAACGCA	4905	0.0	35.05915	5
TACATGG	11705	0.0	34.51925	2
TATCAAC	5085	0.0	34.053753	2
AACGCAG	5340	0.0	32.86869	6
ACATGGG	12130	0.0	32.770164	3
CATGGGG	10510	0.0	32.725636	4
ATCAACG	5225	0.0	32.68576	3
ATGGGGG	6235	0.0	29.393871	5
ACGCAGA	6285	0.0	27.633526	7
CGCAGAG	6705	0.0	25.54954	8
ATGGGGA	4260	0.0	23.466171	5
GTATAAT	725	0.0	22.166182	1
GCAGAGT	7780	0.0	22.019236	9
AGAGTAC	6640	0.0	21.052044	10-11
GTATATA	605	0.0	19.676132	1
GAGTACA	7925	0.0	19.151642	1
>>END_MODULE