FastQCFastQC Report
Mon 15 Aug 2016
SRR2921601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128275
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29200.051.470721
GTATCAA49350.045.5613671
TCAACGC61850.035.6657334
CAACGCA63400.034.7618565
ATCAACG66650.033.102613
AACGCAG67200.032.9835176
TATCAAC67850.032.6058122
GTACATG107700.031.8415871
TACATGG113000.029.8154222
ACATGGG116150.028.5954343
CATGGGG101450.028.032094
ACGCAGA80300.027.52657
CGCAGAG84700.026.095948
ATGGGGG54900.025.9308075
GTATATA6450.025.8905261
GTATACG1401.0395175E-425.560291
CCCTATA3950.024.1542682
GCAGAGT97050.022.5295759
AGAGTAC87050.022.4485110-11
CAGAGTA91850.020.01052510-11
Produced by FastQC (version 0.11.5)