FastQCFastQC Report
Mon 15 Aug 2016
SRR2921600_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921600_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820576
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG41300.034.1512954
GTACATG53950.034.094311
ATGGGGG28300.033.4364055
TACATGG55650.033.044732
ACATGGG56300.031.6061573
GTATCAA24400.030.7394121
TCAACGC27650.027.1196634
CTATAGC2205.138645E-927.051183
CAACGCA27900.026.663355
TGGGGGG23000.026.6480436
AACGCAG29650.025.6886436
ATCAACG29000.025.651983
GGGTTAG2601.0986696E-925.1845511
TATCAAC29700.024.847012
TTAAGAG9350.024.8234375
CCTCTAC7150.024.1379762
TAGATAG2001.8214268E-623.805045
CGGTTAA2502.0023435E-823.8050382
GGTATCA18300.023.4205061
GCGGTTA2802.590241E-923.3856541
Produced by FastQC (version 0.11.5)