##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921600_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	820576
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.015535428771983	31.0	30.0	33.0	23.0	34.0
2	29.40304859025855	31.0	30.0	34.0	23.0	34.0
3	29.47945223842764	31.0	30.0	34.0	23.0	34.0
4	32.875633701205004	37.0	35.0	37.0	25.0	37.0
5	32.91162305502476	37.0	35.0	37.0	26.0	37.0
6	32.891005830051085	37.0	35.0	37.0	25.0	37.0
7	32.84111526537457	37.0	35.0	37.0	23.0	37.0
8	32.74578832429903	37.0	35.0	37.0	23.0	37.0
9	34.41065056740631	39.0	35.0	39.0	22.0	39.0
10-11	34.424864363569	39.0	35.0	39.0	23.0	39.0
12-13	34.33406960476543	39.0	35.0	39.0	21.5	39.0
14-15	35.57759366591273	40.0	37.0	41.0	21.5	41.0
16-17	35.54910148481067	40.0	37.0	41.0	20.5	41.0
18-19	35.495918111180444	40.0	37.0	41.0	19.0	41.0
20-21	35.565880552197484	40.0	37.0	41.0	21.5	41.0
22-23	35.52156229282845	40.0	37.0	41.0	22.0	41.0
24-25	35.545140852279374	40.0	36.5	41.0	23.0	41.0
26-27	35.53342651990797	40.0	36.0	41.0	24.0	41.0
28-29	35.48222163455914	40.0	36.0	41.0	24.0	41.0
30-31	35.378673639004795	40.0	36.0	41.0	23.5	41.0
32-33	35.2780169051983	40.0	36.0	41.0	23.0	41.0
34-35	35.144061610381	40.0	35.5	41.0	21.5	41.0
36-37	35.03528923585384	40.0	35.0	41.0	20.0	41.0
38-39	34.91127756600242	40.0	35.0	41.0	18.0	41.0
40-41	34.7909157713606	40.0	35.0	41.0	17.0	41.0
42-43	34.538388278477555	40.0	35.0	41.0	15.0	41.0
44-45	34.201627880903175	39.5	34.5	41.0	11.0	41.0
46-47	33.937307452326166	39.0	34.0	41.0	8.5	41.0
48-49	33.744281455952894	39.0	34.0	41.0	7.5	41.0
50-51	33.54196381566119	38.5	33.0	40.0	4.0	41.0
52-53	33.35236711773193	38.0	33.0	40.0	2.0	41.0
54-55	33.128254421284566	38.0	33.0	40.0	2.0	41.0
56-57	32.8907584428499	38.0	33.0	40.0	2.0	41.0
58-59	32.91654825390945	38.0	33.0	40.0	2.0	41.0
60-61	32.71730589244628	37.0	33.0	40.0	2.0	41.0
62-63	32.40655953866552	37.0	32.0	40.0	2.0	41.0
64-65	32.102831425730216	36.0	32.0	39.5	2.0	41.0
66-67	31.785625584954957	36.0	32.0	39.0	2.0	41.0
68-69	31.450010114846158	35.0	31.5	39.0	2.0	40.5
70-71	31.07908834769723	35.0	31.0	38.0	2.0	40.0
72-73	30.725018157976837	35.0	31.0	37.0	2.0	39.5
74-75	30.355670285263034	35.0	31.0	37.0	2.0	39.0
76-77	30.047079734235464	35.0	31.0	36.0	2.0	39.0
78-79	29.773164825683423	35.0	30.0	36.0	2.0	37.5
80-81	29.526196842218148	35.0	30.0	35.0	2.0	37.0
82-83	29.313647364777914	35.0	30.0	35.0	2.0	37.0
84-85	29.101427533829895	34.5	30.0	35.0	2.0	36.0
86-87	28.92649309753149	34.0	30.0	35.0	2.0	36.0
88-89	28.773417087509262	34.0	29.5	35.0	2.0	35.5
90-91	28.58955660414148	34.0	29.0	35.0	2.0	35.0
92-93	28.514464839332373	34.0	29.0	35.0	2.0	35.0
94-95	28.40217542311742	34.0	29.0	35.0	2.0	35.0
96-97	28.31484469445853	34.0	29.0	35.0	2.0	35.0
98-99	28.231949264906604	34.0	29.0	35.0	2.0	35.0
100-101	28.139244871894867	34.0	29.0	35.0	2.0	35.0
102-103	27.291746285536014	32.5	26.5	34.5	2.0	35.0
104-105	28.172459345630386	34.0	29.0	35.0	2.0	35.0
106-107	28.434328447334558	34.0	29.5	35.0	2.0	35.0
108-109	28.4390348974379	34.0	30.0	35.0	2.0	35.0
110-111	28.387941519128027	34.0	30.0	35.0	2.0	35.0
112-113	28.313802134110674	34.0	29.5	35.0	2.0	35.0
114-115	28.2053204090785	34.0	29.0	35.0	2.0	35.0
116-117	28.09595150235932	34.0	29.0	35.0	2.0	35.0
118-119	27.824564696018406	34.0	29.0	35.0	2.0	35.0
120-121	27.702722234137973	34.0	29.0	35.0	2.0	35.0
122-123	27.480873800842335	34.0	29.0	35.0	2.0	35.0
124-125	26.733163046445426	33.5	26.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45896.0
3	7315.0
4	3676.0
5	3059.0
6	3105.0
7	3396.0
8	3440.0
9	3555.0
10	4021.0
11	4314.0
12	4625.0
13	4793.0
14	4586.0
15	3965.0
16	3181.0
17	2835.0
18	2645.0
19	2385.0
20	2343.0
21	2342.0
22	2780.0
23	4021.0
24	6381.0
25	7987.0
26	7818.0
27	8037.0
28	9612.0
29	11464.0
30	13836.0
31	17166.0
32	22283.0
33	30512.0
34	48456.0
35	76931.0
36	148041.0
37	229303.0
38	60421.0
39	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.77557359104196	18.04992454513761	12.48503702042279	24.689464843397637
2	16.07503927593339	19.403956478975033	39.786733814598094	24.73427043049348
3	19.910151360587747	24.772971306205672	27.89354179387708	27.423335539329496
4	13.35642567588979	15.494092439248533	34.761125885768465	36.38835599909321
5	13.96327602703411	38.318351626251605	31.85064202963026	15.867730317084023
6	33.232782438031904	34.194460471056345	17.834102000812376	14.738655090099376
7	28.99524086491153	33.770837220021285	19.287605153961618	17.946316761105564
8	27.0929600269202	36.403715182357516	18.20380833014306	18.299516460579223
9	28.036329417271467	17.328025237023727	17.88459522515121	36.7510501205536
10-11	26.3619094531391	26.653982335412575	25.985951727198685	20.998156484249645
12-13	25.60143037960027	23.87360348770813	26.486276655841724	24.038689476849875
14-15	23.674957533913442	25.02844785920336	23.865849221165572	27.43074538571762
16-17	24.273384452219283	27.879033536102206	24.35353707744633	23.494044934232182
18-19	24.036862561506688	27.222165328027543	25.820007900165322	22.92096421030045
20-21	24.925459190887857	26.249579450872545	25.424754618726503	23.40020673951309
22-23	24.826340209830278	25.63167301001363	25.539326719347738	24.002660060808356
24-25	24.14109627364968	25.604891181452423	25.704362920690183	24.549649624207714
26-27	23.840988145617608	25.737896593892	25.777216128202408	24.643899132287984
28-29	24.29104386627978	26.731486674306893	25.34740679329936	23.630062666113968
30-31	25.212754481054596	26.00343381435583	25.441065957393644	23.34274574719593
32-33	24.589301637551724	25.935084272933008	25.611681978397993	23.863932111117272
34-35	24.356718105647516	26.440225425263115	25.60051936810214	23.602537100987224
36-37	24.435238692433835	26.34260875818021	25.577893519999655	23.644259029386298
38-39	24.640240855182167	26.325495788691022	25.371155885471897	23.663107470654918
40-41	24.590546669267532	25.978555057056475	25.623597971325466	23.80730030235053
42-43	24.49371134919092	26.149841572440003	25.23438076124458	24.1220663171245
44-45	23.772897523185474	26.309130732074753	25.989183971215763	23.92878777352401
46-47	23.653668825964722	26.04218279862824	25.787491673324958	24.51665670208208
48-49	23.957099113067983	26.307054498290988	25.560366475042546	24.175479913598487
50-51	24.019501479859766	26.41305884819613	25.726581889637078	23.84085778230702
52-53	24.21154959622124	26.3260703946366	25.47048605820509	23.991893950937072
54-55	23.824716978257225	25.90842741996922	26.03867075010285	24.228184851670704
56-57	24.10309200437849	26.131618018123444	25.486668275688896	24.278621701809175
58-59	23.87648478059893	26.338001302944303	25.8970235218621	23.88849039459467
60-61	24.44581505959332	26.245194382211473	25.824786626913408	23.4842039312818
62-63	24.317415971725765	26.51361816006587	25.636539051077317	23.532426817131046
64-65	24.124583813538713	25.95839445730748	25.669021831543443	24.24799989761036
66-67	24.068912511755734	25.96141011489685	25.711697951287537	24.25797942205988
68-69	23.83136559881619	25.95959054219761	26.096169926450692	24.112873932535514
70-71	23.854677971143833	26.086617264443323	26.049065722513614	24.00963904189923
72-73	23.850873835637476	26.771683776081602	25.407054555915	23.970387832365926
74-75	24.016731200251552	26.37071406198393	25.39448362772592	24.218071110038597
76-77	23.877875509710318	25.89258344440158	25.834444015813435	24.395097030074663
78-79	23.754978653120055	25.970544048319216	26.175266874091502	24.099210424469224
80-81	24.043457751980977	26.19068804247534	25.680467170224357	24.08538703531933
82-83	24.372719443823122	26.319301938781294	25.603176063964973	23.70480255343061
84-85	24.213328263142884	26.749825102805424	25.1845256812459	23.85232095280579
86-87	23.81989863950719	26.686355337027294	25.521124082502272	23.972621940963244
88-89	23.598345434189444	26.210253212928137	25.810629372504636	24.38077198037778
90-91	24.34804495762521	25.906947320691792	25.765677698048677	23.979330023634322
92-93	24.068338026555452	26.475135266563033	25.545529942066487	23.91099676481503
94-95	23.992097414840625	26.371038804813217	25.46377717732431	24.173086603021847
96-97	23.73343376522704	26.324613187902102	25.59843209237784	24.343520954493016
98-99	23.716744809027546	26.202269073784507	25.46388774800094	24.61709836918701
100-101	23.505440992486758	26.549800757966786	25.66428738141915	24.280470868127306
102-103	23.318391935057324	26.36602282158435	25.83088677076897	24.484698472589358
104-105	23.90165636642613	26.43812360532387	25.514575317690618	24.145644710559385
106-107	23.779930487174738	26.37222021819033	25.80109143910072	24.046757855534214
108-109	23.694702022270324	26.362887341796835	25.756279373622732	24.186131262310106
110-111	23.377622058039453	26.885642470866024	25.792002437352423	23.944733033742096
112-113	23.399280728971803	26.932020235134114	25.69224657030076	23.976452465593326
114-115	23.488401996673836	27.173814345410435	25.36989717410077	23.967886483814958
116-117	23.475303586488632	26.906769332767844	25.426556688165125	24.191370392578403
118-119	23.32425394427968	26.74649930285445	25.753326563506235	24.17592018935964
120-121	23.370791172445394	26.7580831303233	25.934384395180405	23.936741302050898
122-123	23.215410807405124	26.95794493319863	25.642939577146045	24.1837046822502
124-125	23.157677276766776	26.840550230607402	25.566983718738424	24.434788773887394
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	619.0
1	472.0
2	308.5
3	265.0
4	223.0
5	203.0
6	202.0
7	242.5
8	284.0
9	316.0
10	339.5
11	329.0
12	333.5
13	382.5
14	421.0
15	438.0
16	503.5
17	551.0
18	573.5
19	616.0
20	682.5
21	802.0
22	934.5
23	1131.5
24	1382.0
25	1706.0
26	2161.0
27	2670.0
28	3216.5
29	3944.0
30	4917.5
31	5852.0
32	7034.0
33	8321.5
34	9469.5
35	10699.5
36	12152.5
37	13860.5
38	15295.5
39	16792.5
40	18447.0
41	19883.5
42	21319.0
43	23329.0
44	25672.0
45	27837.5
46	29799.5
47	31844.5
48	34537.5
49	37538.5
50	39971.5
51	40104.5
52	38106.0
53	36374.5
54	35150.5
55	33174.5
56	30716.5
57	28126.0
58	25229.0
59	22190.0
60	18806.5
61	15733.5
62	12981.0
63	10124.0
64	7662.0
65	5798.0
66	4423.0
67	3473.5
68	2759.0
69	2107.0
70	1618.0
71	1235.0
72	933.0
73	754.5
74	618.0
75	474.5
76	367.0
77	262.5
78	169.5
79	115.0
80	78.5
81	56.0
82	43.5
83	44.5
84	50.0
85	38.5
86	20.5
87	15.0
88	17.5
89	18.5
90	16.0
91	12.0
92	7.5
93	9.5
94	9.0
95	8.5
96	9.0
97	5.5
98	5.5
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02656670436376399
2	0.012064696018406583
3	0.010358577389541006
4	0.012430292867449205
5	0.015964395741527904
6	0.09249600280778379
7	0.057154974066996836
8	0.045577740513980426
9	0.023641929571423
10-11	0.04923370900440666
12-13	0.011516300744842648
14-15	0.026262040322895135
16-17	0.03241625394844597
18-19	0.041434309558164024
20-21	0.026810435596459074
22-23	0.03582849120617713
24-25	0.02955241196427875
26-27	0.04545587489763288
28-29	0.043384159419724684
30-31	0.025043384159419726
32-33	0.028699352649845963
34-35	0.04247016729711812
36-37	0.009078988417891821
38-39	0.020229692313691844
40-41	0.04094684709277385
42-43	0.020656221970908238
44-45	0.015476933276137736
46-47	0.020778087587255783
48-49	0.026810435596459074
50-51	0.027785360527239407
52-53	0.024494988885855787
54-55	0.02297166868151152
56-57	0.02461685450220333
58-59	0.015416000467963969
60-61	0.022179542175252503
62-63	0.022240474983426278
64-65	0.02199674375073119
66-67	0.028211890184455794
68-69	0.021204617244472174
70-71	0.045090278048590254
72-73	0.020717154779082012
74-75	0.00889618999337051
76-77	0.016390925398744295
78-79	0.024190324844986937
80-81	0.017914245603088564
82-83	0.023093534297859066
84-85	0.011455367936668875
86-87	0.01681745505596069
88-89	0.021874878134383654
90-91	0.01937663299925906
92-93	0.008104063487111492
94-95	0.010358577389541006
96-97	0.014562941153531178
98-99	0.00792126506259018
100-101	0.011150703895800024
102-103	0.004509027804859026
104-105	0.005483952735639356
106-107	9.749249307803299E-4
108-109	0.01364894903092462
110-111	0.0016451858206918066
112-113	0.0028029091759934487
114-115	0.013588016222750849
116-117	0.006337012050072145
118-119	0.2728571150021448
120-121	0.001096790547127871
122-123	0.001096790547127871
124-125	0.30697948757945637
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	820576.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.49824575941666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.8530524277463	62.24195694102836
2	8.36102465733643	12.12319240816238
3	2.436427788849582	5.299102218332675
4	1.0861279048735848	3.149694710947419
5	0.625425644456231	2.267113103801469
6	0.39020501983243494	1.6973507654621955
7	0.2617033718083515	1.3281124755800904
8	0.18407239188539615	1.0675940403544883
9	0.1405935186797083	0.9173505122480367
>10	0.6348275184541922	7.7740257688853385
>50	0.018813929170693414	0.9207754413771739
>100	0.00722196864321737	0.9598561740994829
>500	5.038582639479912E-4	0.25387543972092075
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2186561634754124E-4	0.0
3	0.0	0.0	0.0	1.2186561634754124E-4	0.0
4	0.0	0.0	0.0	1.2186561634754124E-4	0.0
5	0.0	0.0	0.0	1.2186561634754124E-4	0.0
6	0.0	0.0	0.0	1.2186561634754124E-4	0.0
7	0.0	0.0	0.0	1.2186561634754124E-4	0.0
8	0.0	0.0	0.0	1.2186561634754124E-4	0.0
9	0.0	0.0	0.0	1.2186561634754124E-4	0.0
10-11	0.0	0.0	0.0	1.2186561634754124E-4	0.0
12-13	0.0	0.0	0.0	1.2186561634754124E-4	1.8279842452131187E-4
14-15	0.0	0.0	0.0	1.2186561634754124E-4	2.4373123269508248E-4
16-17	0.0	0.0	0.0	1.2186561634754124E-4	2.4373123269508248E-4
18-19	0.0	0.0	0.0	1.2186561634754124E-4	3.6559684904262374E-4
20-21	0.0	0.0	0.0	1.2186561634754124E-4	3.6559684904262374E-4
22-23	0.0	0.0	0.0	3.046640408688531E-4	3.6559684904262374E-4
24-25	0.0	0.0	0.0	4.8746246539016497E-4	3.6559684904262374E-4
26-27	0.0	0.0	0.0	6.702608899114769E-4	3.6559684904262374E-4
28-29	0.0	0.0	0.0	0.0015233202043442653	3.6559684904262374E-4
30-31	0.0	0.0	0.0	0.006458877666419685	3.6559684904262374E-4
32-33	1.2186561634754124E-4	0.0	0.0	0.01864543930117381	3.6559684904262374E-4
34-35	1.2186561634754124E-4	0.0	0.0	0.033634910111921386	3.6559684904262374E-4
36-37	1.2186561634754124E-4	0.0	0.0	0.05368180400109192	3.6559684904262374E-4
38-39	1.2186561634754124E-4	0.0	0.0	0.08853537027648872	3.6559684904262374E-4
40-41	1.2186561634754124E-4	0.0	0.0	0.13466150606403307	3.6559684904262374E-4
42-43	1.2186561634754124E-4	0.0	0.0	0.17012440042116758	3.6559684904262374E-4
44-45	1.2186561634754124E-4	0.0	0.0	0.21503188004523652	3.6559684904262374E-4
46-47	1.2186561634754124E-4	0.0	0.0	0.25969562843661037	3.6559684904262374E-4
48-49	1.2186561634754124E-4	0.0	0.0	0.31075732168623016	3.6559684904262374E-4
50-51	1.2186561634754124E-4	0.0	0.0	0.37473677026868935	3.6559684904262374E-4
52-53	1.2186561634754124E-4	0.0	0.0	0.44736867761182386	3.6559684904262374E-4
54-55	1.2186561634754124E-4	0.0	0.0	0.5192084584486995	3.6559684904262374E-4
56-57	1.2186561634754124E-4	0.0	0.0	0.5875750692196701	3.6559684904262374E-4
58-59	1.2186561634754124E-4	0.0	0.0	0.672332605389385	3.6559684904262374E-4
60-61	1.2186561634754124E-4	0.0	0.0	0.7655598018952541	3.6559684904262374E-4
62-63	1.2186561634754124E-4	0.0	0.0	0.8891924696798347	3.6559684904262374E-4
64-65	1.2186561634754124E-4	0.0	0.0	0.9891832078929922	3.6559684904262374E-4
66-67	1.2186561634754124E-4	0.0	0.0	1.109281772803494	3.6559684904262374E-4
68-69	1.2186561634754124E-4	0.0	0.0	1.2261508988807863	3.6559684904262374E-4
70-71	1.2186561634754124E-4	0.0	0.0	1.3557549818663963	3.6559684904262374E-4
72-73	1.2186561634754124E-4	0.0	0.0	1.4712226533556916	4.8746246539016497E-4
74-75	1.2186561634754124E-4	0.0	0.0	1.598633155247046	4.8746246539016497E-4
76-77	1.2186561634754124E-4	0.0	0.0	1.7583380454704987	4.8746246539016497E-4
78-79	1.2186561634754124E-4	0.0	0.0	1.9405880747182467	4.8746246539016497E-4
80-81	1.2186561634754124E-4	0.0	0.0	2.1026084116523025	4.8746246539016497E-4
82-83	1.2186561634754124E-4	0.0	0.0	2.266152068790703	6.093280817377062E-4
84-85	1.2186561634754124E-4	0.0	0.0	2.4480974339975825	6.093280817377062E-4
86-87	1.2186561634754124E-4	0.0	0.0	2.665383827945248	6.093280817377062E-4
88-89	1.2186561634754124E-4	0.0	0.0	2.900828198728698	6.093280817377062E-4
90-91	1.2186561634754124E-4	0.0	0.0	3.144132901766564	6.093280817377062E-4
92-93	1.2186561634754124E-4	0.0	0.0	3.355508813321374	6.093280817377062E-4
94-95	1.2186561634754124E-4	0.0	0.0	3.583824045548493	7.311936980852475E-4
96-97	1.2186561634754124E-4	0.0	0.0	3.8468709784346604	7.311936980852475E-4
98-99	1.2186561634754124E-4	0.0	0.0	4.163721580938268	7.311936980852475E-4
100-101	1.2186561634754124E-4	0.0	0.0	4.506651425340249	7.311936980852475E-4
102-103	1.2186561634754124E-4	0.0	0.0	4.869810962055922	8.530593144327887E-4
104-105	1.2186561634754124E-4	0.0	0.0	5.23516407986585	8.530593144327887E-4
106-107	1.2186561634754124E-4	0.0	0.0	5.623793530398159	8.530593144327887E-4
108-109	1.2186561634754124E-4	0.0	0.0	6.050688784463596	8.530593144327887E-4
110-111	1.2186561634754124E-4	0.0	0.0	6.5225524509612764	9.139921226065593E-4
112-113	1.2186561634754124E-4	0.0	0.0	6.943293491401162	9.749249307803299E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	4130	0.0	34.151295	4
GTACATG	5395	0.0	34.09431	1
ATGGGGG	2830	0.0	33.436405	5
TACATGG	5565	0.0	33.04473	2
ACATGGG	5630	0.0	31.606157	3
GTATCAA	2440	0.0	30.739412	1
TCAACGC	2765	0.0	27.119663	4
CTATAGC	220	5.138645E-9	27.05118	3
CAACGCA	2790	0.0	26.66335	5
TGGGGGG	2300	0.0	26.648043	6
AACGCAG	2965	0.0	25.688643	6
ATCAACG	2900	0.0	25.65198	3
GGGTTAG	260	1.0986696E-9	25.184551	1
TATCAAC	2970	0.0	24.84701	2
TTAAGAG	935	0.0	24.823437	5
CCTCTAC	715	0.0	24.137976	2
TAGATAG	200	1.8214268E-6	23.80504	5
CGGTTAA	250	2.0023435E-8	23.805038	2
GGTATCA	1830	0.0	23.420506	1
GCGGTTA	280	2.590241E-9	23.385654	1
>>END_MODULE