FastQCFastQC Report
Mon 15 Aug 2016
SRR2921600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820576
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29200.35584759973482044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.2830938267753383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18560.22618258394103652No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38200.041.410431
GGTATCA24950.036.3663941
CATGGGG41350.034.339164
TCAACGC46500.033.615274
CAACGCA47100.033.3893665
TATCAAC47200.033.3756562
GTACATG55500.033.2347761
ATCAACG48050.032.7832373
AACGCAG48550.032.770256
TACATGG56700.032.519462
TAAGACG750.003858551631.8190354
ATGGGGG28150.031.5306575
ACATGGG57000.031.508883
GTACTAG1403.0156898E-629.8467641
TACACCG1004.407231E-429.784835
ACGCAGA56150.028.4373877
ACCGTAG850.007110636728.0790523
CGCAGAG57600.027.8249558
TACTAGG2005.9430022E-826.8522322
GGGTTAG2705.638867E-1126.5304571
Produced by FastQC (version 0.11.5)