##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921600_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	820576
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60981310689077	33.0	31.0	34.0	30.0	34.0
2	31.795487559957884	33.0	31.0	34.0	30.0	34.0
3	31.858885709550364	34.0	31.0	34.0	30.0	34.0
4	35.252800471863665	37.0	35.0	37.0	33.0	37.0
5	35.10424872284834	37.0	35.0	37.0	33.0	37.0
6	35.1299513999922	37.0	35.0	37.0	33.0	37.0
7	35.01821159770698	37.0	35.0	37.0	33.0	37.0
8	35.05416926646648	37.0	35.0	37.0	33.0	37.0
9	36.73811688374995	39.0	38.0	39.0	33.0	39.0
10-11	36.68583653433686	39.0	38.0	39.0	33.0	39.0
12-13	36.602669953788556	39.0	38.0	39.0	33.0	39.0
14-15	37.98962740806458	41.0	38.0	41.0	33.0	41.0
16-17	37.999056150801394	41.0	38.5	41.0	33.0	41.0
18-19	38.02870788324299	41.0	39.0	41.0	33.0	41.0
20-21	37.981037710096324	41.0	39.0	41.0	33.0	41.0
22-23	37.987420421752525	41.0	39.0	41.0	33.0	41.0
24-25	37.946472965331665	41.0	39.0	41.0	33.0	41.0
26-27	37.861032372382326	41.0	38.0	41.0	33.0	41.0
28-29	37.77969012011075	41.0	38.0	41.0	33.0	41.0
30-31	37.63122367702687	40.5	38.0	41.0	32.5	41.0
32-33	37.46512388858558	40.0	38.0	41.0	32.0	41.0
34-35	37.335783035331275	40.0	38.0	41.0	32.0	41.0
36-37	37.25741735073899	40.0	38.0	41.0	31.5	41.0
38-39	37.16391595659634	40.0	37.5	41.0	31.0	41.0
40-41	37.029088103966	40.0	37.0	41.0	31.0	41.0
42-43	36.85371982607339	40.0	37.0	41.0	30.5	41.0
44-45	36.6720553611122	40.0	37.0	41.0	30.0	41.0
46-47	36.398206869321065	40.0	36.5	41.0	29.5	41.0
48-49	36.14802346156846	40.0	36.0	41.0	28.5	41.0
50-51	35.88725907167648	40.0	35.5	41.0	28.0	41.0
52-53	35.77301005635066	40.0	35.0	41.0	27.5	41.0
54-55	35.65853193366611	40.0	35.0	41.0	27.0	41.0
56-57	35.4776187702297	39.0	35.0	41.0	27.0	41.0
58-59	35.294182988534885	39.0	35.0	41.0	27.0	41.0
60-61	35.04216245661584	39.0	35.0	41.0	26.0	41.0
62-63	34.68446310884062	38.0	35.0	41.0	26.0	41.0
64-65	34.39806306789377	37.0	35.0	40.0	26.0	41.0
66-67	34.09751930351363	37.0	35.0	39.5	26.0	41.0
68-69	33.721792375112116	36.0	34.5	39.0	25.5	41.0
70-71	33.34580099832313	36.0	34.0	39.0	24.5	41.0
72-73	33.01840962933355	35.0	34.0	38.0	24.5	40.0
74-75	32.68113617751433	35.0	34.0	37.0	24.0	39.0
76-77	32.34433800159888	35.0	34.0	37.0	23.0	39.0
78-79	32.072078028116835	35.0	34.0	36.0	23.0	38.5
80-81	31.81862191923722	35.0	34.0	36.0	22.0	37.0
82-83	31.585747084974457	35.0	33.5	35.5	20.5	37.0
84-85	31.363117493078033	35.0	33.0	35.0	20.0	36.5
86-87	31.22024650976875	35.0	33.0	35.0	20.0	36.0
88-89	31.121220947237063	35.0	33.0	35.0	20.0	36.0
90-91	31.00562105155403	35.0	33.0	35.0	19.0	36.0
92-93	30.918391471356706	35.0	33.0	35.0	18.5	35.0
94-95	30.847896477596223	35.0	33.0	35.0	18.0	35.0
96-97	30.77889372830792	35.0	33.0	35.0	17.5	35.0
98-99	30.700122231213196	35.0	33.0	35.0	16.0	35.0
100-101	30.629360961080998	35.0	33.0	35.0	14.0	35.0
102-103	29.94268294466326	34.0	31.5	35.0	10.5	35.0
104-105	30.452993994462425	35.0	32.5	35.0	10.0	35.0
106-107	30.546999302928675	35.0	33.0	35.0	10.0	35.0
108-109	30.52959872089849	35.0	33.0	35.0	8.5	35.0
110-111	30.47039457649261	35.0	33.0	35.0	7.0	35.0
112-113	30.440012259681005	35.0	33.0	35.0	3.5	35.0
114-115	30.312289172483716	35.0	33.0	35.0	2.0	35.0
116-117	30.488726821159773	35.0	33.0	35.0	2.0	35.0
118-119	30.478069063682096	35.0	33.0	35.0	2.0	35.0
120-121	30.400280412783218	35.0	33.0	35.0	2.0	35.0
122-123	30.270688516359243	35.0	33.0	35.0	2.0	35.0
124-125	29.63988161974028	34.5	31.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	14388.0
3	3918.0
4	2122.0
5	1625.0
6	1708.0
7	1923.0
8	2316.0
9	2368.0
10	2675.0
11	2946.0
12	3322.0
13	4432.0
14	6551.0
15	6332.0
16	3562.0
17	3215.0
18	2802.0
19	2319.0
20	2039.0
21	2147.0
22	2144.0
23	2513.0
24	3469.0
25	4696.0
26	5358.0
27	5926.0
28	7030.0
29	8331.0
30	10325.0
31	12827.0
32	16457.0
33	22317.0
34	35636.0
35	62365.0
36	137434.0
37	298453.0
38	112293.0
39	292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.51132011535864	18.45994261321944	11.575479218623157	26.453258052798763
2	15.400205227200159	19.89277975611599	38.65705364911998	26.049961367563867
3	18.551387514079938	24.64733102853047	28.827183670683006	27.974097786706587
4	13.012319095330168	15.32404152262651	34.229189859573054	37.43444952247027
5	13.853681369716739	38.05374134236336	32.2218890408521	15.870688247067802
6	33.60581167896742	34.54580709362393	16.912722376964016	14.935658850444632
7	29.604137734268598	32.75657524943334	19.20651971142181	18.43276730487625
8	28.218407831002878	35.791799099517576	17.880659866110236	18.109133203369314
9	28.59441628449594	15.8616090911529	18.207540039545833	37.336434584805325
10-11	26.95387879656248	25.73879589180159	25.938840400332904	21.36848491130303
12-13	26.58672675572724	22.873837359593033	26.902352837891225	23.637083046788504
14-15	24.162534591459544	23.949026256891617	24.82049631237818	27.06794283927066
16-17	23.93300470508195	27.09482221520477	25.318010624535926	23.654162455177357
18-19	23.752342928546394	25.974246834189234	27.687731435624706	22.58567880163967
20-21	24.544438952473186	25.162194299171308	27.524913470979335	22.768453277376175
22-23	24.161720797026383	24.6245810736701	27.63798671622692	23.575711413076593
24-25	23.602832298439434	24.544839647303284	27.818130853650363	24.034197200606922
26-27	23.369447321918226	24.73676192797514	27.817683261227227	24.076107488879412
28-29	23.734900921772713	25.79970348740492	27.389208428367468	23.0761871624549
30-31	24.577541514635236	25.152762645724717	27.427262556760436	22.842433282879615
32-33	24.146880543471173	24.944805232396934	27.65210629814498	23.25620792598691
34-35	23.887306263843737	25.376199282791568	27.566457557043055	23.17003689632164
36-37	24.06271841974249	25.196931962421598	27.640551866519523	23.099797751316384
38-39	24.18861566678752	25.253927969703525	27.363286546037298	23.194169817471657
40-41	24.170951399611724	25.001553837735468	27.548080614320412	23.279414148332396
42-43	24.032758815052237	25.078652233126252	27.177629434185313	23.710959517636198
44-45	23.447065970942145	25.39329498845571	27.61611167341328	23.543527367188865
46-47	23.431384556886588	25.233306582673247	27.184276705415517	24.151032155024645
48-49	23.680015696616575	25.6241781517234	26.812209725321395	23.88359642633863
50-51	23.830360271155456	25.716810115012567	26.842074796252568	23.610754817579405
52-53	23.984527762222807	25.831666293956673	26.439112491187487	23.74469345263303
54-55	23.590645127873888	25.281858787254656	27.110117179644988	24.01737890522647
56-57	23.82485292191075	25.464937375657698	26.65472371255259	24.055485989878957
58-59	23.705971781724234	25.593263198944634	27.07924698455642	23.621518034774713
60-61	24.193873689867658	25.563704985098873	27.01305364344902	23.22936768158445
62-63	24.09897778124421	25.76178939466321	26.883756129900267	23.255476694192314
64-65	23.843190636820964	25.17079466131108	26.92870617712436	24.057308524743597
66-67	23.85859398326722	25.415303243575917	26.799017726023557	23.92708504713331
68-69	23.555978566197037	25.27026845874773	27.09064275817021	24.083110216885032
70-71	23.60457776001248	25.420375443590842	27.079697675778963	23.895349120617713
72-73	23.68219168065614	26.05119080625063	26.42453453901768	23.842082974075552
74-75	23.74589521111518	25.8121677855498	26.387645628982536	24.05429137435248
76-77	23.553824761352292	25.325417610634677	26.686498627158738	24.434259000854293
78-79	23.461123883328085	25.354919023196555	27.04032357817602	24.143633515299342
80-81	23.669471200717545	25.59994442927895	26.584618609367077	24.145965760636432
82-83	24.045889907608384	25.69744640347873	26.43282565271916	23.823838036193724
84-85	23.903080763795924	26.0317502874563	26.150388850534302	23.914780098213477
86-87	23.418976426315176	26.125733631010412	26.430153940646573	24.025136002027843
88-89	23.22383377824954	25.61766779413978	26.67717972888242	24.48131869872826
90-91	23.930923457897457	25.320814362734108	26.62917637538876	24.119085803979683
92-93	23.6765394064657	25.82015559801895	26.429239948523964	24.07406504699138
94-95	23.577930331839614	25.66781671251007	26.264430922299393	24.489822033350922
96-97	23.336889832413945	25.57145181329659	26.46796987679808	24.62368847749138
98-99	23.392064793905707	25.474151535942653	26.209797811523927	24.923985858627713
100-101	23.212292340989745	25.80199762118317	26.35874068946691	24.626969348360177
102-103	23.11570496200486	25.65182182287703	26.358895176558118	24.873578038559994
104-105	23.680682837421518	25.70730803728113	26.143099481339938	24.468909643957414
106-107	23.599571029893855	25.51561719292687	26.34284704534653	24.54196473183275
108-109	23.494295625701888	25.522619155705073	26.299715619133156	24.68336959945988
110-111	23.235076909577927	25.99820369643688	26.364288900208876	24.402430493776308
112-113	23.20785401917074	25.94648742617206	26.279790902338423	24.565867652318772
114-115	23.24294154350115	26.094109503568223	26.0679083960535	24.59504055687712
116-117	23.232999048207496	25.853414205228887	26.151321602277477	24.76226514428614
118-119	23.089288119027266	25.63720863346176	26.479386120188682	24.794117127322295
120-121	23.02953931537425	25.712372364650314	26.568504924437924	24.68958339553751
122-123	22.831106710335895	25.799801965115392	26.345707974712468	25.02338334983624
124-125	22.73500551109875	25.773886968493105	26.182202353394707	25.308905167013435
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	24.5
2	45.0
3	117.0
4	109.5
5	95.0
6	229.0
7	611.0
8	994.5
9	1119.0
10	990.0
11	818.0
12	801.0
13	845.0
14	905.5
15	970.0
16	1001.5
17	1006.5
18	1073.5
19	1097.0
20	1078.0
21	1144.0
22	1254.0
23	1435.5
24	1666.5
25	1911.5
26	2255.0
27	2746.5
28	3284.5
29	3963.5
30	4918.0
31	5875.5
32	6908.0
33	8026.5
34	9262.0
35	10526.5
36	11999.0
37	13652.0
38	15025.5
39	16464.0
40	18074.5
41	19592.0
42	21048.0
43	22903.0
44	25210.0
45	27781.0
46	29762.5
47	31554.0
48	34277.0
49	37170.5
50	40077.0
51	40837.0
52	38624.0
53	36793.0
54	35691.0
55	33759.0
56	30995.0
57	27945.0
58	25135.5
59	21994.5
60	18512.0
61	15482.5
62	12664.5
63	9634.0
64	6976.5
65	5266.0
66	4046.5
67	3128.5
68	2385.5
69	1771.5
70	1346.5
71	1000.0
72	765.5
73	632.0
74	466.0
75	342.5
76	263.0
77	165.5
78	95.5
79	54.0
80	30.5
81	12.5
82	9.0
83	8.5
84	5.5
85	4.5
86	3.5
87	2.5
88	3.0
89	3.0
90	1.0
91	0.5
92	2.0
93	1.5
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.021692079709862342
2	0.002681043559645907
3	0.03168506025036072
4	0.1657372382326561
5	0.02461685450220333
6	0.06970713255079358
7	0.0508179620169247
8	0.042531100105291895
9	0.03192879148305581
10-11	0.06196866591272472
12-13	0.05605818351986897
14-15	0.05697217564247553
16-17	0.04862438092266896
18-19	0.035158230316265644
20-21	0.02272793744881644
22-23	0.0031685060250360724
24-25	0.004996490270249191
26-27	0.0031685060250360724
28-29	0.004569960613032797
30-31	0.002681043559645907
32-33	0.00883525718519674
34-35	0.004082498147642632
36-37	0.006276079241898374
38-39	0.002254513902429513
40-41	0.003290371641383613
42-43	0.004082498147642632
44-45	0.004935557462075421
46-47	0.0043262293803377145
48-49	0.0020717154779082013
50-51	0.0016451858206918066
52-53	0.0011577233553016417
54-55	0.004996490270249191
56-57	0.0010358577389541007
58-59	0.0010358577389541007
60-61	6.093280817377062E-5
62-63	9.749249307803299E-4
64-65	0.0
66-67	0.0037778341067737785
68-69	3.6559684904262374E-4
70-71	0.0
72-73	0.002254513902429513
74-75	0.004935557462075421
76-77	0.0018279842452131186
78-79	1.8279842452131187E-4
80-81	0.0
82-83	0.005727683968334438
84-85	0.0020107826697344303
86-87	0.0
88-89	0.0011577233553016417
90-91	9.749249307803299E-4
92-93	0.0
94-95	0.0035341028740786956
96-97	0.0015233202043442656
98-99	6.093280817377061E-4
100-101	0.0
102-103	0.001279588971649183
104-105	0.0
106-107	7.311936980852475E-4
108-109	0.002620110751472137
110-111	2.4373123269508248E-4
112-113	3.0466404086885307E-4
114-115	0.0
116-117	0.0023154467106032833
118-119	0.00298570760051476
120-121	0.0031075732168623013
122-123	0.0016451858206918066
124-125	0.10559655656514448
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	820576.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.46538671745172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.45281177170433	57.13629030608443
2	9.70854135388699	13.2939807651249
3	2.8615108672744456	5.877433443724067
4	1.3250977516649574	3.628933200246684
5	0.7493335655402922	2.565170617254153
6	0.4528983896825289	1.860471803599529
7	0.29090426154831023	1.3941810935261847
8	0.2260255176333771	1.237993957822509
9	0.1603339806827923	0.9879595192256228
>10	0.7368672684016825	8.49331410020164
>50	0.023368235535704322	1.0868196351718546
>100	0.011236859382607649	1.3203269739504027
>500	5.350885309991332E-4	0.25021734507600374
>1k	5.350885309991332E-4	0.8669072389919718
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2920	0.35584759973482044	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2323	0.2830938267753383	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1856	0.22618258394103652	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	2.4373123269508248E-4	1.2186561634754124E-4
24-25	0.0	0.0	0.0	4.8746246539016497E-4	2.4373123269508248E-4
26-27	0.0	0.0	0.0	6.702608899114769E-4	2.4373123269508248E-4
28-29	0.0	0.0	0.0	0.0014014545879967243	2.4373123269508248E-4
30-31	0.0	0.0	0.0	0.006337012050072144	3.046640408688531E-4
32-33	0.0	0.0	0.0	0.01834077526030496	3.6559684904262374E-4
34-35	0.0	0.0	0.0	0.033208380454704985	3.6559684904262374E-4
36-37	0.0	0.0	0.0	0.053438072768396835	3.6559684904262374E-4
38-39	0.0	0.0	0.0	0.08762137815388216	3.6559684904262374E-4
40-41	0.0	0.0	0.0	0.13283352181881994	3.6559684904262374E-4
42-43	0.0	0.0	0.0	0.16780895371056428	3.6559684904262374E-4
44-45	0.0	0.0	0.0	0.212777366142807	4.2652965721639435E-4
46-47	0.0	0.0	0.0	0.2583551066567874	4.8746246539016497E-4
48-49	0.0	0.0	0.0	0.3091121358655383	6.093280817377062E-4
50-51	0.0	0.0	0.0	0.37296971883165	6.093280817377062E-4
52-53	0.0	0.0	0.0	0.44450483562765664	6.093280817377062E-4
54-55	0.0	0.0	0.0	0.5161618180400109	6.093280817377062E-4
56-57	0.0	0.0	0.0	0.5849549584681979	6.093280817377062E-4
58-59	0.0	0.0	0.0	0.66885943532348	6.093280817377062E-4
60-61	0.0	0.0	0.0	0.7622084974456966	6.093280817377062E-4
62-63	0.0	0.0	0.0	0.8849271731076707	8.530593144327887E-4
64-65	0.0	0.0	0.0	0.9854663065943923	8.530593144327887E-4
66-67	0.0	0.0	0.0	1.106417930819327	8.530593144327887E-4
68-69	0.0	0.0	0.0	1.2226777288148813	8.530593144327887E-4
70-71	0.0	0.0	0.0	1.3514896852942324	9.749249307803299E-4
72-73	0.0	0.0	0.0	1.467383886440744	0.001096790547127871
74-75	0.0	0.0	0.0	1.5954646492220097	0.001096790547127871
76-77	0.0	0.0	0.0	1.7567537924579808	0.001096790547127871
78-79	0.0	0.0	0.0	1.939978746636509	0.001096790547127871
80-81	0.0	0.0	0.0	2.1043145302811683	0.0011577233553016417
82-83	0.0	0.0	0.0	2.2671269937214835	0.0012186561634754125
84-85	0.0	0.0	0.0	2.4492551573528836	0.0012186561634754125
86-87	0.0	0.0	0.0	2.667821140272199	0.0012186561634754125
88-89	0.0	0.0	0.0	2.9057637561907734	0.0012186561634754125
90-91	0.0	0.0	0.0	3.1546133447724527	0.0012186561634754125
92-93	0.0	0.0	0.0	3.3688530983114298	0.0012186561634754125
94-95	0.0	0.0	0.0	3.599422844440978	0.0012186561634754125
96-97	0.0	0.0	0.0	3.8642977615723586	0.0013405217798229537
98-99	0.0	0.0	0.0	4.1842559372928285	0.001462387396170495
100-101	0.0	0.0	0.0	4.5304152205280195	0.001462387396170495
102-103	0.0	0.0	0.0	4.894062219709083	0.001462387396170495
104-105	0.0	0.0	0.0	5.259598135943532	0.001462387396170495
106-107	0.0	0.0	0.0	5.6514570253090515	0.001462387396170495
108-109	0.0	0.0	0.0	6.081216121358656	0.0015233202043442656
110-111	0.0	0.0	0.0	6.556248293881371	0.0017061186288655774
112-113	0.0	0.0	0.0	6.980828101236205	0.0017061186288655774
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3820	0.0	41.41043	1
GGTATCA	2495	0.0	36.366394	1
CATGGGG	4135	0.0	34.33916	4
TCAACGC	4650	0.0	33.61527	4
CAACGCA	4710	0.0	33.389366	5
TATCAAC	4720	0.0	33.375656	2
GTACATG	5550	0.0	33.234776	1
ATCAACG	4805	0.0	32.783237	3
AACGCAG	4855	0.0	32.77025	6
TACATGG	5670	0.0	32.51946	2
TAAGACG	75	0.0038585516	31.819035	4
ATGGGGG	2815	0.0	31.530657	5
ACATGGG	5700	0.0	31.50888	3
GTACTAG	140	3.0156898E-6	29.846764	1
TACACCG	100	4.407231E-4	29.78483	5
ACGCAGA	5615	0.0	28.437387	7
ACCGTAG	85	0.0071106367	28.079052	3
CGCAGAG	5760	0.0	27.824955	8
TACTAGG	200	5.9430022E-8	26.852232	2
GGGTTAG	270	5.638867E-11	26.530457	1
>>END_MODULE