FastQCFastQC Report
Mon 15 Aug 2016
SRR2921599_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921599_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735476
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27850.041.2512551
GGTATCA19400.039.5815771
GTACATG73000.037.5093961
TACATGG76400.035.055942
ACATGGG78900.033.869733
CATGGGG66850.033.8317644
TCAACGC32500.033.695894
CAACGCA33700.032.318495
TATCAAC36050.031.203192
AACGCAG36200.030.2483436
ATCAACG36550.030.124983
ATGGGGG41900.029.5446765
ACGCAGA42850.025.5422387
CGCAGAG46800.023.766368
GTATTAC4101.8189894E-1221.7777211
GTATAAG7350.020.2468621
GCAGAGT54800.019.9717779
AGTACAT52850.019.707682
AGAGTAC49400.019.50652910-11
GAGTACA55800.019.4152181
Produced by FastQC (version 0.11.5)