FastQCFastQC Report
Mon 15 Aug 2016
SRR2921599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735476
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.1473947205262418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21890.12613254231115845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27000.054.5505071
GTATCAA45400.050.698731
TCAACGC60100.037.589574
TATCAAC61850.036.8193742
CAACGCA61750.036.5608675
ATCAACG63250.035.8158953
AACGCAG64850.035.1059536
GTACATG85450.034.0544131
TACATGG89300.032.1104472
ACATGGG90050.031.048593
CATGGGG76800.030.5800064
ACGCAGA76750.029.5834527
CGCAGAG80500.028.3534018
ATGGGGG45650.026.489245
GCAGAGT89100.025.2154039
AGAGTAC80100.024.92381710-11
CGTATAC1451.3230622E-424.6681183
GAGTACT42550.022.2477612-13
AGTACTT46050.022.0436512-13
GTGGTAT17000.021.7474521
Produced by FastQC (version 0.11.5)