FastQCFastQC Report
Mon 15 Aug 2016
SRR2921598_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921598_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684894
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCG13200.045.5359732
GGTATCA21000.044.5018271
GTATCAA32400.042.8063741
GTACATG88100.036.822911
TACATGG89900.035.151352
TCAACGC39850.034.348414
ACATGGG91800.033.9700163
CATGGGG77800.033.35144
ATCAACG42250.032.6789743
CAACGCA41850.032.5627755
TATCAAC44050.031.478732
AACGCAG44650.030.9160046
ATGGGGG49650.029.8443035
GCCTCGG16750.027.7132193
ACGCAGA53700.025.368097
GTAAGTA8350.024.9453012
CCTCGGT10650.024.0284654
CGCAGAG57900.023.6292538
TCGGTCT6700.023.0895466
CGTATAC1350.002552691122.0416153
Produced by FastQC (version 0.11.5)