##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921598_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1684894
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.72810337030104	31.0	31.0	34.0	25.0	34.0
2	29.693398516464537	31.0	31.0	34.0	25.0	34.0
3	29.792253993426293	31.0	31.0	34.0	25.0	34.0
4	33.31923966730251	37.0	35.0	37.0	27.0	37.0
5	33.28315609171853	37.0	35.0	37.0	27.0	37.0
6	33.36152422644985	37.0	35.0	37.0	27.0	37.0
7	33.176352340265915	37.0	35.0	37.0	26.0	37.0
8	33.18304474940263	37.0	35.0	37.0	26.0	37.0
9	34.83202207379218	39.0	37.0	39.0	27.0	39.0
10-11	34.89044355312559	39.0	37.0	39.0	27.0	39.0
12-13	34.83863406243954	39.0	37.0	39.0	27.0	39.0
14-15	36.0961567908723	40.0	38.0	41.0	27.0	41.0
16-17	36.05585989385682	40.0	38.0	41.0	26.5	41.0
18-19	36.000675710163364	40.0	37.5	41.0	26.0	41.0
20-21	36.068189749622235	40.0	38.0	41.0	26.0	41.0
22-23	35.98630982127065	40.0	37.0	41.0	25.5	41.0
24-25	35.97931323869632	40.0	37.0	41.0	26.0	41.0
26-27	35.95362645958737	40.0	37.0	41.0	26.0	41.0
28-29	35.89426219097463	40.0	37.0	41.0	26.0	41.0
30-31	35.78578207293752	40.0	37.0	41.0	25.0	41.0
32-33	35.70356829569101	40.0	37.0	41.0	25.0	41.0
34-35	35.56542162296263	40.0	36.5	41.0	24.5	41.0
36-37	35.43705479395143	40.0	36.0	41.0	24.0	41.0
38-39	35.31227335369465	40.0	36.0	41.0	23.0	41.0
40-41	35.199061780741104	40.0	35.5	41.0	22.5	41.0
42-43	34.90814436991289	40.0	35.0	41.0	20.0	41.0
44-45	34.49448511301007	39.5	35.0	41.0	17.5	41.0
46-47	34.208529141892605	39.0	34.5	41.0	15.0	41.0
48-49	33.99964982960353	39.0	34.0	40.5	13.5	41.0
50-51	33.784202151589355	38.5	34.0	40.0	9.5	41.0
52-53	33.57376428428138	38.0	33.5	40.0	8.5	41.0
54-55	33.324972965658375	38.0	33.0	40.0	7.5	41.0
56-57	33.06809300763134	38.0	33.0	40.0	6.5	41.0
58-59	33.06998096022657	37.5	33.0	40.0	2.0	41.0
60-61	32.855079607381825	37.0	33.0	40.0	2.0	41.0
62-63	32.54878674860258	36.5	33.0	40.0	2.0	41.0
64-65	32.241884059175234	36.0	32.5	39.0	2.0	41.0
66-67	31.91444179871256	35.5	32.0	39.0	2.0	41.0
68-69	31.577901636542123	35.0	32.0	38.5	2.0	40.0
70-71	31.22506074566115	35.0	32.0	37.5	2.0	40.0
72-73	30.89239441769037	35.0	31.0	37.0	2.0	39.0
74-75	30.536665808056767	35.0	31.0	36.5	2.0	39.0
76-77	30.257618876914513	35.0	31.0	36.0	2.0	38.5
78-79	29.996944021404314	35.0	31.0	35.5	2.0	37.0
80-81	29.75005430608691	35.0	31.0	35.0	2.0	37.0
82-83	29.540492458279275	35.0	30.5	35.0	2.0	36.5
84-85	29.340742503682726	34.5	30.0	35.0	2.0	36.0
86-87	29.18031163978268	34.0	30.0	35.0	2.0	36.0
88-89	29.02720171120557	34.0	30.0	35.0	2.0	35.5
90-91	28.84750821119904	34.0	30.0	35.0	2.0	35.0
92-93	28.776260702453683	34.0	30.0	35.0	2.0	35.0
94-95	28.657093561968885	34.0	29.0	35.0	2.0	35.0
96-97	28.56728553843743	34.0	29.0	35.0	2.0	35.0
98-99	28.476751356465154	34.0	29.0	35.0	2.0	35.0
100-101	28.373489074090124	34.0	29.0	35.0	2.0	35.0
102-103	27.481358767969972	32.5	27.0	34.5	2.0	35.0
104-105	28.41925990596441	34.0	29.5	35.0	2.0	35.0
106-107	28.71355883515521	34.0	30.0	35.0	2.0	35.0
108-109	28.716848953109217	34.0	30.0	35.0	2.0	35.0
110-111	28.660796465534332	34.0	30.0	35.0	2.0	35.0
112-113	28.58452549537241	34.0	30.0	35.0	2.0	35.0
114-115	28.474548547267663	34.0	30.0	35.0	2.0	35.0
116-117	28.358095227355548	34.0	30.0	35.0	2.0	35.0
118-119	28.0755614299772	34.0	29.0	35.0	2.0	35.0
120-121	27.94779493546775	34.0	29.0	35.0	2.0	35.0
122-123	27.706081213417583	34.0	29.0	35.0	2.0	35.0
124-125	26.92698947233476	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86864.0
3	13689.0
4	6896.0
5	6029.0
6	6391.0
7	6947.0
8	6803.0
9	7039.0
10	7642.0
11	8225.0
12	8382.0
13	8278.0
14	8443.0
15	7064.0
16	5966.0
17	5312.0
18	5163.0
19	4718.0
20	4699.0
21	4949.0
22	5598.0
23	7547.0
24	10763.0
25	13420.0
26	14900.0
27	16501.0
28	19737.0
29	23546.0
30	28923.0
31	36277.0
32	48012.0
33	66774.0
34	103402.0
35	171222.0
36	329097.0
37	458518.0
38	111113.0
39	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.05634632246391	18.064114749028057	12.46272657168757	25.41681235682047
2	16.503217151811576	20.233095113728098	38.00109216961869	25.262595564841632
3	20.49552958522457	23.383447801830904	28.151356022847402	27.969666590097127
4	13.819505162006823	15.19404195254622	33.96406390880821	37.02238897663875
5	14.836382372617354	37.30162546174855	32.18738369100853	15.67460847462557
6	34.309760389327245	33.73209038836485	17.127464038200834	14.830685184107065
7	29.6130454844815	31.62338699667972	19.230556810913757	19.533010707925026
8	28.340423308322464	34.40633038570889	18.54996354066463	18.703282765304014
9	27.885117152459383	15.890429338369264	18.19536472003272	38.02908878913863
10-11	26.564327359499202	25.45499998960831	26.032510539396814	21.94816211149567
12-13	26.278807276578053	22.93544326752106	26.448217761923658	24.337531693977233
14-15	24.243041305278993	24.2742389597853	23.563638911044535	27.919080823891168
16-17	24.83672897484795	27.091739317325253	24.243105213036035	23.828426494790765
18-19	24.663680458286404	26.444806900896396	25.53929581453841	23.35221682627879
20-21	25.258290605242305	25.292249456257647	25.597018270396166	23.852441668103882
22-23	25.63242945413977	24.710896602007175	25.234301850249874	24.42237209360318
24-25	25.098842799355133	25.272350168446117	25.177952660540328	24.450854371658426
26-27	24.539508646923476	25.566956134491203	25.52806353447636	24.365471684108957
28-29	24.79392429595811	25.652190822613534	25.445469392432564	24.10841548899579
30-31	25.229761923843892	25.230860198009925	25.363246759645964	24.176131118500223
32-33	24.65820803855629	25.514984761586433	25.323165089888384	24.503642109968894
34-35	24.729112243440785	25.82718107804079	25.350409126090284	24.09329755242814
36-37	25.000037097899575	25.638833250283504	25.287530980455937	24.073598671360983
38-39	24.912772793719345	25.757425999718027	25.232415425599015	24.097385780963616
40-41	24.82993237670728	25.588727590766563	25.253698677637765	24.327641354888392
42-43	24.824571531865043	25.465763658873442	25.245018086770788	24.46464672249073
44-45	24.620521602858815	25.429905349293456	25.476800337169024	24.4727727106787
46-47	24.53847457591902	25.43583523969046	25.511822382428452	24.51386780196207
48-49	24.566720128141306	25.757091363315098	25.3473882689317	24.328800239611894
50-51	24.512474293883248	25.75601511258448	25.37591485279241	24.35559574073986
52-53	24.703386195610907	25.529787829595218	25.333108930605235	24.433717044188636
54-55	24.515418770256122	25.39923058269069	25.436065968881966	24.649284678171217
56-57	24.511795305624197	25.597007947639085	25.15817360137136	24.73302314536536
58-59	24.492243673173185	25.677278036082914	25.332684313862252	24.49779397688165
60-61	24.77669275838354	25.596665848425044	25.483635711705478	24.143005681485942
62-63	24.61660889506544	25.623194204595173	25.379146539610698	24.381050360728686
64-65	24.496121776111377	25.496576501275726	25.381500165624438	24.62580155698846
66-67	24.6219424049346	25.66490494573098	25.21237864978264	24.50077399955178
68-69	24.44829124558887	25.69939208075433	25.325813074552844	24.526503599103957
70-71	24.431494636036028	25.55493735856765	25.42926259523582	24.584305410160507
72-73	24.5555337557483	25.855458571148286	25.154101716185707	24.43490595691771
74-75	24.447621334256127	25.823830610899684	25.244007000501266	24.48454105434292
76-77	24.49061182380111	25.47982659580594	25.221162767367577	24.808398813025377
78-79	24.459574902594955	25.661175889498182	25.259237227813525	24.620011980093334
80-81	24.611009651929212	25.810503533361768	25.10840203717769	24.47008477753133
82-83	24.584404015842292	25.766550300285818	25.29207110687373	24.356974576998162
84-85	24.586926481342537	25.772368533520744	25.099009430826364	24.54169555431035
86-87	24.371089529734412	25.95477804556625	25.201830723385054	24.472301701314276
88-89	24.3590261126846	25.667628570258245	25.29363190535866	24.679713411698497
90-91	24.603316416636343	25.505052154044243	25.29306411465757	24.598567314661842
92-93	24.649682209174504	25.587829929081717	25.276419849521236	24.486068012222542
94-95	24.564035580549355	25.56903087788841	25.29188699723895	24.575046544323286
96-97	24.435151286704965	25.46193309223228	25.304866194852586	24.798049426210167
98-99	24.26304655698628	25.59574756020689	25.240596068077974	24.90060981472886
100-101	24.301877676082402	25.762094549495153	25.30177943991137	24.63424833451108
102-103	24.110621380936216	25.68834919647584	25.449571113490517	24.751458309097426
104-105	24.154256332579347	25.76128355627518	25.301679290178946	24.782780820966526
106-107	24.118265188297407	25.731441718656196	25.34687301635819	24.803420076688205
108-109	24.104064668088117	25.713406352839495	25.344252622481395	24.83827635659099
110-111	23.97176876693564	26.115760163312157	25.31706423902357	24.595406830728635
112-113	23.980059993328798	26.135617685568008	25.31056103377503	24.57376128732816
114-115	23.95368943285315	26.232682434151034	25.23797694443925	24.57565118855657
116-117	23.89906683175075	26.192071690493464	25.271308489207605	24.63755298854818
118-119	23.94611865134138	25.949899380929338	25.353684515019655	24.75029745270963
120-121	23.865981431219364	26.179007176217052	25.408651463524507	24.546359929039077
122-123	23.84654077225199	26.230927767745726	25.27489387210643	24.647637587895858
124-125	23.82235075250052	26.16209402736111	25.25316306842421	24.762392151714156
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	769.0
1	644.5
2	451.5
3	327.5
4	237.5
5	199.0
6	213.0
7	259.0
8	324.0
9	362.0
10	355.5
11	330.0
12	344.5
13	402.5
14	443.5
15	472.0
16	529.5
17	614.0
18	690.0
19	773.0
20	874.5
21	980.0
22	1097.5
23	1299.5
24	1596.5
25	2019.0
26	2686.0
27	3693.5
28	4858.0
29	6218.0
30	8008.0
31	10031.0
32	12526.5
33	14922.5
34	17296.0
35	19944.0
36	23081.0
37	26660.5
38	29791.5
39	33446.0
40	37144.0
41	40465.0
42	43975.0
43	48272.5
44	52763.5
45	56596.0
46	60894.5
47	65238.5
48	69292.5
49	72614.5
50	74597.0
51	75342.5
52	74409.5
53	73763.0
54	73545.0
55	71653.5
56	67641.0
57	62025.5
58	56492.5
59	50777.0
60	44171.5
61	37909.5
62	32155.5
63	25562.0
64	19203.0
65	14878.0
66	11629.0
67	9037.0
68	7170.5
69	5663.0
70	4536.0
71	3588.5
72	2820.0
73	2314.0
74	1876.0
75	1446.0
76	1089.5
77	765.0
78	522.5
79	350.5
80	240.0
81	184.5
82	154.5
83	112.0
84	87.5
85	86.0
86	75.0
87	61.5
88	47.0
89	39.0
90	35.0
91	35.0
92	35.0
93	28.0
94	22.0
95	20.0
96	19.5
97	11.5
98	9.5
99	9.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022731400313610234
2	0.010327059150308565
3	0.008961988113198814
4	0.010742515552907186
5	0.01572799238409063
6	0.09377444515797434
7	0.05941026557160273
8	0.049023855506637216
9	0.027598175315479787
10-11	0.050774707489017114
12-13	0.013561683999112109
14-15	0.02804330717540688
16-17	0.03430482867171466
18-19	0.041961096662460666
20-21	0.029823834615115254
22-23	0.037687830807160566
24-25	0.0313076074815389
26-27	0.0456408533711913
28-29	0.044127405047439186
30-31	0.025698946046457522
32-33	0.029645781871144417
34-35	0.04483961602332254
36-37	0.009644523631753688
38-39	0.02115860107520117
40-41	0.04219850032108845
42-43	0.02228626845368314
44-45	0.016558905189287872
46-47	0.023651339490792893
48-49	0.029883185529772198
50-51	0.027894929888764516
52-53	0.02507576144255959
54-55	0.0221082157097123
56-57	0.0256099196744721
58-59	0.01789430076906915
60-61	0.02299847942956649
62-63	0.02296880397223802
64-65	0.02136632927650048
66-67	0.027390447114180476
68-69	0.022404970282997032
70-71	0.046590468005702435
72-73	0.02216756662436925
74-75	0.00934776905846896
76-77	0.016321501530660087
78-79	0.025698946046457522
80-81	0.018309757171667773
82-83	0.02466030503996097
84-85	0.012226288419330829
86-87	0.018636187202280975
88-89	0.02296880397223802
90-91	0.021663083849785207
92-93	0.008190426222658517
94-95	0.01243401662063014
96-97	0.01552026418279132
98-99	0.007122109758833493
100-101	0.01059413826626482
102-103	0.003917160367358421
104-105	0.004451318599270933
106-107	0.0011573428358104428
108-109	0.01365071037109753
110-111	0.00133539557978128
112-113	0.0023740365862778313
114-115	0.013324280340484327
116-117	0.005697687807066794
118-119	0.284587635780055
120-121	0.0013650710371097528
122-123	9.792900918396052E-4
124-125	0.32010915820223707
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1684894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.69058032199337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.42313305621587	52.67309729041485
2	10.31561776005116	13.346465985551417
3	3.315129682874842	6.433729890835181
4	1.562225878196154	4.042451946181797
5	0.8858622289734404	2.8653470838813204
6	0.5529562645435675	2.146263698760303
7	0.39044667257431775	1.768075528353676
8	0.2664131287614236	1.3787535923979546
9	0.21218439401263553	1.2353698425553097
>10	1.0287026488820636	11.415621204176444
>50	0.038247472495348565	1.642232207458693
>100	0.008805716542241857	0.9044094374423184
>500	2.7509587701725547E-4	0.1481822919907602
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.935091465694578E-5	0.0	0.0	0.0
6	0.0	5.935091465694578E-5	0.0	0.0	1.7805274397083732E-4
7	0.0	5.935091465694578E-5	0.0	0.0	2.374036586277831E-4
8	0.0	5.935091465694578E-5	0.0	0.0	2.374036586277831E-4
9	0.0	5.935091465694578E-5	0.0	5.935091465694578E-5	2.9675457328472886E-4
10-11	0.0	5.935091465694578E-5	0.0	5.935091465694578E-5	2.9675457328472886E-4
12-13	0.0	5.935091465694578E-5	0.0	5.935091465694578E-5	3.8578094527014754E-4
14-15	0.0	5.935091465694578E-5	0.0	5.935091465694578E-5	5.04482774584039E-4
16-17	0.0	5.935091465694578E-5	0.0	5.935091465694578E-5	6.528600612264035E-4
18-19	0.0	5.935091465694578E-5	0.0	8.902637198541866E-5	7.418864332118222E-4
20-21	0.0	5.935091465694578E-5	0.0	1.1870182931389155E-4	9.496146345111325E-4
22-23	0.0	5.935091465694578E-5	0.0	1.7805274397083732E-4	0.0011276673784819699
24-25	0.0	5.935091465694578E-5	0.0	7.418864332118222E-4	0.0012463692077958612
26-27	0.0	5.935091465694578E-5	0.0	0.0018102028970368462	0.0012463692077958612
28-29	0.0	5.935091465694578E-5	0.0	0.004837099544541081	0.00133539557978128
30-31	0.0	5.935091465694578E-5	0.0	0.013591359456440584	0.0013650710371097528
32-33	0.0	5.935091465694578E-5	0.0	0.0324055994026924	0.0014837728664236444
34-35	0.0	5.935091465694578E-5	0.0	0.05525570154561652	0.0015431237810805902
36-37	0.0	5.935091465694578E-5	0.0	0.08959020567465965	0.0015431237810805902
38-39	0.0	5.935091465694578E-5	0.0	0.14125517688353095	0.001602474695737536
40-41	0.0	5.935091465694578E-5	0.0	0.19707471211838845	0.001602474695737536
42-43	0.0	5.935091465694578E-5	0.0	0.2548231520795967	0.0016618256103944817
44-45	0.0	5.935091465694578E-5	0.0	0.3229580021057704	0.0017805274397083733
46-47	0.0	5.935091465694578E-5	0.0	0.3943868278954047	0.001869553811693792
48-49	0.0	5.935091465694578E-5	0.0	0.46602338188633824	0.001958580183679211
50-51	0.0	5.935091465694578E-5	0.0	0.5392030596583524	0.001958580183679211
52-53	0.0	5.935091465694578E-5	0.0	0.6148161249313013	0.002136632927650048
54-55	0.0	5.935091465694578E-5	0.0	0.7074035517961368	0.002403712043606304
56-57	0.0	8.902637198541866E-5	0.0	0.8067273074745355	0.0024333875009347767
58-59	0.0	1.1870182931389155E-4	0.0	0.9140040857169649	0.0024333875009347767
60-61	0.0	1.1870182931389155E-4	0.0	1.0173340281347076	0.0024333875009347767
62-63	0.0	1.1870182931389155E-4	0.0	1.1243734027185093	0.0027301420742195056
64-65	0.0	1.7805274397083732E-4	0.0	1.2385348870611446	0.0027598175315479787
66-67	0.0	1.7805274397083732E-4	0.0	1.3729350333017982	0.0027894929888764514
68-69	0.0	1.7805274397083732E-4	0.0	1.5151398248198404	0.0027894929888764514
70-71	0.0	1.7805274397083732E-4	0.0	1.667790377317505	0.002848843903533397
72-73	0.0	1.7805274397083732E-4	0.0	1.816405067618497	0.0031159230194896534
74-75	0.0	1.7805274397083732E-4	0.0	1.9777208536560758	0.0031752739341465988
76-77	0.0	1.7805274397083732E-4	0.0	2.163667269276287	0.0032643003061320177
78-79	0.0	1.7805274397083732E-4	0.0	2.367804740238852	0.0032643003061320177
80-81	0.0	1.7805274397083732E-4	0.0	2.5746130023609792	0.0033830021354459092
82-83	0.0	1.7805274397083732E-4	0.0	2.787712461436743	0.0035610548794167466
84-85	0.0	1.7805274397083732E-4	0.0	3.012474375242597	0.0035610548794167466
86-87	0.0	1.7805274397083732E-4	0.0	3.2651015434798865	0.0036204057940736924
88-89	0.0	1.7805274397083732E-4	0.0	3.5488285909974158	0.003679756708730638
90-91	0.0	1.7805274397083732E-4	0.0	3.8442477687023633	0.003679756708730638
92-93	0.0	1.7805274397083732E-4	0.0	4.136046540613237	0.003679756708730638
94-95	0.0	1.7805274397083732E-4	0.0	4.449894177319166	0.003917160367358422
96-97	0.0	1.7805274397083732E-4	0.0	4.792823762206999	0.004095213111329259
98-99	0.0	1.7805274397083732E-4	0.0	5.178337628361191	0.004154564025986205
100-101	0.0	1.7805274397083732E-4	0.0	5.587443483091517	0.00421391494064315
102-103	0.0	2.9675457328472886E-4	0.0	5.99548102135802	0.004451318599270934
104-105	0.0	2.9675457328472886E-4	0.0	6.423757221522541	0.004570020428584825
106-107	0.0	2.9675457328472886E-4	0.0	6.890967621702018	0.004570020428584825
108-109	0.0	2.9675457328472886E-4	0.0	7.387586400094012	0.004718397715227189
110-111	0.0	2.9675457328472886E-4	0.0	7.91812422621245	0.0047777486298841345
112-113	0.0	2.9675457328472886E-4	0.0	8.453321099131458	0.004807424087212608
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCTCG	1320	0.0	45.535973	2
GGTATCA	2100	0.0	44.501827	1
GTATCAA	3240	0.0	42.806374	1
GTACATG	8810	0.0	36.82291	1
TACATGG	8990	0.0	35.15135	2
TCAACGC	3985	0.0	34.34841	4
ACATGGG	9180	0.0	33.970016	3
CATGGGG	7780	0.0	33.3514	4
ATCAACG	4225	0.0	32.678974	3
CAACGCA	4185	0.0	32.562775	5
TATCAAC	4405	0.0	31.47873	2
AACGCAG	4465	0.0	30.916004	6
ATGGGGG	4965	0.0	29.844303	5
GCCTCGG	1675	0.0	27.713219	3
ACGCAGA	5370	0.0	25.36809	7
GTAAGTA	835	0.0	24.945301	2
CCTCGGT	1065	0.0	24.028465	4
CGCAGAG	5790	0.0	23.629253	8
TCGGTCT	670	0.0	23.089546	6
CGTATAC	135	0.0025526911	22.041615	3
>>END_MODULE