FastQCFastQC Report
Mon 15 Aug 2016
SRR2921598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684894
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30730.18238536074079437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26630.15805148573144662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18760.11134231589643027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27800.057.940481
GTATCAA47950.050.5126951
TCAACGC62100.037.7451064
TATCAAC63300.037.501792
CAACGCA62900.037.2174155
ATCAACG64250.036.575963
AACGCAG65750.035.7970856
GTACATG86350.033.3002741
TACATGG89750.031.69982
ACATGGG92600.030.9173933
CATGGGG77350.030.1492254
ACGCAGA77900.029.983487
ATGGGGG46100.029.71885
CGCAGAG83000.028.1402858
GCAGAGT90550.025.6623659
GAGTACT40400.025.0503512-13
GTATCGC1802.15356E-523.1724456
AGAGTAC82950.023.12916810-11
GTACTTT44200.022.95756714-15
CAGAGTA86950.022.40777210-11
Produced by FastQC (version 0.11.5)