##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921598_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1684894
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.610152923566705	33.0	31.0	34.0	30.0	34.0
2	31.78915765620864	33.0	31.0	34.0	30.0	34.0
3	31.85391069111766	34.0	31.0	34.0	30.0	34.0
4	35.24069110579063	37.0	35.0	37.0	33.0	37.0
5	35.07561306527295	37.0	35.0	37.0	33.0	37.0
6	35.100051397892095	37.0	35.0	37.0	33.0	37.0
7	34.98521984172298	37.0	35.0	37.0	33.0	37.0
8	35.02266195974346	37.0	35.0	37.0	33.0	37.0
9	36.7271585037397	39.0	38.0	39.0	33.0	39.0
10-11	36.68662449982017	39.0	38.0	39.0	33.0	39.0
12-13	36.61170554349413	39.0	38.0	39.0	33.0	39.0
14-15	38.018020421462715	41.0	39.0	41.0	33.0	41.0
16-17	38.02714176678177	41.0	39.0	41.0	33.0	41.0
18-19	38.045558355599816	41.0	39.0	41.0	33.0	41.0
20-21	37.98728822109878	41.0	39.0	41.0	33.0	41.0
22-23	37.98835624080803	41.0	39.0	41.0	33.0	41.0
24-25	37.94642541311204	41.0	39.0	41.0	33.0	41.0
26-27	37.86610196249734	41.0	39.0	41.0	33.0	41.0
28-29	37.791861387125834	41.0	38.0	41.0	33.0	41.0
30-31	37.66486704801608	41.0	38.0	41.0	32.5	41.0
32-33	37.534055554830154	41.0	38.0	41.0	32.0	41.0
34-35	37.39538392326164	40.5	38.0	41.0	32.0	41.0
36-37	37.30562189668905	40.0	38.0	41.0	32.0	41.0
38-39	37.21878023187215	40.0	38.0	41.0	31.0	41.0
40-41	37.088182401979	40.0	38.0	41.0	31.0	41.0
42-43	36.91581221133199	40.0	37.0	41.0	31.0	41.0
44-45	36.73481981655819	40.0	37.0	41.0	30.0	41.0
46-47	36.499183331414315	40.0	37.0	41.0	30.0	41.0
48-49	36.28692606181754	40.0	36.0	41.0	29.5	41.0
50-51	36.03227829169075	40.0	35.5	41.0	28.0	41.0
52-53	35.89797548095014	40.0	35.0	41.0	28.0	41.0
54-55	35.76125115289152	39.5	35.0	41.0	28.0	41.0
56-57	35.56121572039547	39.0	35.0	41.0	28.0	41.0
58-59	35.362090434175684	39.0	35.0	41.0	28.0	41.0
60-61	35.106139614717605	38.5	35.0	41.0	28.0	41.0
62-63	34.75933322808438	37.5	35.0	40.5	26.5	41.0
64-65	34.47110382018097	37.0	35.0	40.0	27.0	41.0
66-67	34.16685203935678	36.5	35.0	39.5	26.0	41.0
68-69	33.80580321373333	36.0	35.0	39.0	26.0	41.0
70-71	33.44002471372086	35.5	34.0	39.0	26.0	41.0
72-73	33.12809826612238	35.0	34.0	37.5	26.0	40.0
74-75	32.81414884259782	35.0	34.0	37.0	26.0	39.0
76-77	32.498653624501	35.0	34.0	36.5	25.0	39.0
78-79	32.24346724482371	35.0	34.0	36.0	25.0	38.0
80-81	31.995386356649142	35.0	34.0	36.0	24.0	37.0
82-83	31.775295656581363	35.0	34.0	35.0	24.0	37.0
84-85	31.568869317595052	35.0	33.5	35.0	23.5	36.0
86-87	31.450476409791953	35.0	33.0	35.0	23.0	36.0
88-89	31.348019816083387	35.0	33.0	35.0	23.0	36.0
90-91	31.24374263306772	35.0	33.0	35.0	22.5	35.5
92-93	31.165219295694566	35.0	33.0	35.0	20.5	35.0
94-95	31.097076433294912	35.0	33.0	35.0	20.0	35.0
96-97	31.030393009886673	35.0	33.0	35.0	20.0	35.0
98-99	30.956963464763955	35.0	33.0	35.0	19.5	35.0
100-101	30.886445675514302	35.0	33.0	35.0	19.0	35.0
102-103	30.1971156642495	34.0	31.5	35.0	18.5	35.0
104-105	30.71212996188484	35.0	33.0	35.0	18.0	35.0
106-107	30.811821989988687	35.0	33.0	35.0	18.0	35.0
108-109	30.796791074097243	35.0	33.0	35.0	18.0	35.0
110-111	30.728756823871414	35.0	33.0	35.0	18.0	35.0
112-113	30.686056214812325	35.0	33.0	35.0	17.0	35.0
114-115	30.561193463802468	35.0	33.0	35.0	10.0	35.0
116-117	30.751154375290078	35.0	33.0	35.0	12.5	35.0
118-119	30.733665738022687	35.0	33.0	35.0	4.5	35.0
120-121	30.6579387189936	35.0	33.0	35.0	2.0	35.0
122-123	30.522526639658047	35.0	33.0	35.0	2.0	35.0
124-125	29.878584646867992	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	31934.0
3	8546.0
4	4464.0
5	3623.0
6	3767.0
7	4140.0
8	4433.0
9	4733.0
10	4920.0
11	5383.0
12	6112.0
13	8147.0
14	10922.0
15	10104.0
16	5387.0
17	4830.0
18	4437.0
19	3794.0
20	3723.0
21	3614.0
22	4008.0
23	5123.0
24	6718.0
25	8619.0
26	10294.0
27	11941.0
28	14432.0
29	17379.0
30	20943.0
31	26177.0
32	34545.0
33	46661.0
34	71757.0
35	130619.0
36	303639.0
37	614805.0
38	219891.0
39	330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2354102292392	18.393514931863447	11.643677444441476	26.72739739445587
2	16.110924152290128	19.81140306363188	37.20260935162087	26.875063432457118
3	18.601482466582166	23.4114542361913	28.80461660398194	29.182446693244597
4	13.45870414751037	15.239444541220942	34.0631998749106	37.23865143635809
5	14.800584861629645	37.26967214623203	32.52874293336721	15.401000058771114
6	34.63946389531158	34.032586545948476	16.209231924195567	15.118717634544376
7	30.114133694380605	31.78403672230833	19.23895035006146	18.862879233249604
8	28.269143757652337	34.22362697327872	18.37813797779976	19.12909129126918
9	27.88804409650028	15.08009827192921	18.43572367491418	38.59613395665633
10-11	26.886681521460265	25.039433300709803	25.97831999885976	22.095565178970173
12-13	26.851050158675864	22.420299821370886	26.70564677042358	24.023003249529673
14-15	24.320882335561997	23.640703143813344	24.197774860598454	27.840639660026206
16-17	24.350457711017366	26.74007976095466	24.843767814388325	24.065694713639648
18-19	24.299032127156103	25.722030529125394	26.845995616473818	23.13294172724469
20-21	25.071178560234042	24.609972359685035	26.851711134962624	23.467137945118303
22-23	24.98114802237487	24.18926109447415	26.61314614207373	24.21644474107725
24-25	24.498919462087393	24.586852278210763	26.61328376094857	24.30094449875327
26-27	24.064225873750246	24.924784919591534	26.85090824917574	24.16008095748248
28-29	24.2503398744134	25.1357791233273	26.701026552790562	23.91285444946874
30-31	24.749518801892386	24.733018885282252	26.550413477226854	23.96704883559851
32-33	24.408430176048615	24.820067981078314	26.439433892660176	24.332067950212895
34-35	24.501506091137063	24.94505364058582	26.63131185731456	23.92212841096256
36-37	24.514255528153445	24.869345346735635	26.647109275070708	23.969289850040212
38-39	24.548663075260755	25.001046083773947	26.564948744862686	23.885342096102605
40-41	24.55927565822274	24.824938866598607	26.570208447092895	24.045577028085752
42-43	24.43792482081257	24.784488386945135	26.362809734880948	24.414777057361345
44-45	24.386177440674356	24.872938270227344	26.33820648132046	24.402677807777845
46-47	24.447848045217967	24.92083709981559	26.18729511869835	24.444019736268093
48-49	24.361688205123944	25.23930107911395	26.002601411575892	24.396409304186207
50-51	24.463257706039688	25.38698238759401	25.875656989423774	24.27410291694253
52-53	24.614133668471446	25.239223623082392	25.72792384016049	24.418718868285676
54-55	24.3086227951858	25.085299062355787	25.909577206891587	24.696500935566824
56-57	24.3723364193107	25.23061738243002	25.676524641673186	24.720521556586096
58-59	24.38311593888938	25.20884289020161	25.923968427406358	24.48407274350265
60-61	24.62625223085383	25.13085400675295	26.054087707646715	24.188806054746504
62-63	24.42376442944436	25.185718029093472	25.954081582688854	24.436435958773313
64-65	24.298449723973654	25.002945290013876	25.990544802980132	24.70806018303234
66-67	24.3759064376773	25.208070756372997	25.823891938829203	24.592130867120503
68-69	24.23750708134593	25.229355484010924	25.92290450769771	24.61023292694544
70-71	24.1944668906373	25.049446745447117	26.026066335943487	24.730020027972095
72-73	24.392277080223522	25.250867285315355	25.754590157610004	24.602265476851123
74-75	24.226796169059025	25.38063684407956	25.76910840879198	24.623458578069428
76-77	24.246639125414386	24.966458766911206	25.732780609027483	25.054121498646925
78-79	24.107679401124287	25.149408819148782	25.827761507350182	24.915150272376753
80-81	24.333014421085245	25.331741937474998	25.592737584678915	24.742506056760842
82-83	24.28613782139267	25.223046225464024	25.771035255593183	24.71978069755012
84-85	24.28347968067405	25.222963430525297	25.552308008699825	24.941248880100826
86-87	24.000827351750317	25.403526868752575	25.654136105891528	24.94150967360558
88-89	23.972764737076858	25.21060173917998	25.67760981604268	25.139023707700485
90-91	24.065934685536856	25.059566574345528	25.79988657942601	25.07461216069161
92-93	24.181550886880718	25.09528789348173	25.7220632277164	25.001097991921156
94-95	24.13580047238921	25.006922024323387	25.69277370337056	25.164503799916847
96-97	23.936963570418317	24.921789078900588	25.796012385547346	25.345234965133745
98-99	23.80817427436378	24.960538814701433	25.694001988861544	25.537284922073244
100-101	23.841351444067104	25.156330309206393	25.76081937498739	25.24149887173911
102-103	23.747819194412862	24.967586529647235	25.88121757725594	25.403376698683967
104-105	23.822952660523452	25.0811920512507	25.64627210969948	25.449583178526364
106-107	23.894410378415337	24.931604982308844	25.673051784353973	25.500932854921842
108-109	23.80500596046595	24.904301655071677	25.722355946503296	25.568336437959072
110-111	23.602032888735366	25.239199165763047	25.695370566558235	25.463397378943352
112-113	23.61305485818065	25.209746630343822	25.700698561923925	25.476499949551602
114-115	23.587507582079347	25.31263094295546	25.582084095497997	25.517777379467194
116-117	23.48280465949555	25.14487104421998	25.656486100778075	25.71583819550639
118-119	23.496535746049872	24.988923349579085	25.71020396171648	25.804336942654555
120-121	23.380185304824685	25.07363434807524	25.78364010231205	25.762540244788024
122-123	23.358202732142004	25.196595461307513	25.591047609947008	25.854154196603474
124-125	23.151084710436916	25.180854388451934	25.542295805057506	26.125765096053644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	77.0
1	55.0
2	41.0
3	72.5
4	73.0
5	79.5
6	184.5
7	523.5
8	862.5
9	946.5
10	795.0
11	593.0
12	579.5
13	656.0
14	734.5
15	822.5
16	896.5
17	929.5
18	990.5
19	1098.5
20	1191.5
21	1261.0
22	1428.5
23	1601.0
24	1781.0
25	2154.0
26	2826.5
27	3741.0
28	4806.0
29	6090.0
30	7820.5
31	9887.5
32	12342.0
33	14756.5
34	17118.5
35	19703.5
36	22794.5
37	26262.0
38	29617.0
39	33316.5
40	36798.5
41	40148.5
42	43542.5
43	47726.5
44	52537.5
45	56707.5
46	61246.0
47	65583.0
48	69562.0
49	72791.5
50	74994.5
51	76281.5
52	75269.0
53	74641.0
54	74874.5
55	72976.5
56	68630.0
57	62864.0
58	57184.0
59	51454.0
60	44479.0
61	37971.0
62	31906.5
63	24602.0
64	18060.0
65	13933.0
66	10838.0
67	8263.0
68	6556.5
69	5103.0
70	3903.0
71	3007.5
72	2367.5
73	1937.5
74	1539.5
75	1185.5
76	806.5
77	486.0
78	290.5
79	150.0
80	84.0
81	45.0
82	28.0
83	19.0
84	13.0
85	8.5
86	5.5
87	6.5
88	5.5
89	3.0
90	2.5
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024274524094690823
2	0.001839878354365319
3	0.03199014300009377
4	0.17193959976117193
5	0.02332490946017969
6	0.0708056411857363
7	0.053653226849878985
8	0.045581502456534355
9	0.03489833781828412
10-11	0.061606249413909714
12-13	0.05685817624135406
14-15	0.0593212391996173
16-17	0.05116048843428726
18-19	0.03759880443517515
20-21	0.02362166403346442
22-23	0.0034126775927743823
24-25	0.005044827745840391
26-27	0.003501703964759801
28-29	0.004777748629884135
30-31	0.002195983842306994
32-33	0.009525821802439797
34-35	0.004095213111329259
36-37	0.00646924969760709
38-39	0.0022850102142924125
40-41	0.003204949391475072
42-43	0.003917160367358421
44-45	0.0049261259165265
46-47	0.004570020428584825
48-49	0.0023443611289493582
50-51	0.0018102028970368464
52-53	9.792900918396052E-4
54-55	0.00513385411782581
56-57	8.605882625257138E-4
58-59	8.902637198541866E-4
60-61	5.935091465694578E-5
62-63	8.605882625257138E-4
64-65	2.967545732847289E-5
66-67	0.0041842394833146775
68-69	5.638336892409849E-4
70-71	2.967545732847289E-5
72-73	0.0023146856716208856
74-75	0.005104178660497337
76-77	0.0016321501530660088
78-79	2.0772820129931022E-4
80-81	0.0
82-83	0.005786714179052213
84-85	0.001928904726350738
86-87	0.0
88-89	0.0011573428358104428
90-91	8.605882625257138E-4
92-93	0.0
94-95	0.003234624848803545
96-97	0.001602474695737536
98-99	6.231846038979307E-4
100-101	0.0
102-103	0.0013947464944382257
104-105	0.0
106-107	7.418864332118222E-4
108-109	0.0025224138729201956
110-111	5.935091465694578E-5
112-113	2.374036586277831E-4
114-115	0.0
116-117	0.0019882556410076835
118-119	0.0025817647875771414
120-121	0.0028785193608618703
122-123	0.0015134483237521173
124-125	0.10516982077210793
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1684894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.68916588973535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5470893241957	47.66957334151973
2	11.792126993590578	14.313087026136898
3	3.915173101646556	7.1282576955857255
4	1.7890317437271117	4.342993771082287
5	1.016146291577623	3.083453542889687
6	0.6482470302136044	2.3604942932497033
7	0.4339207206214336	1.8434000617751383
8	0.32452073873613946	1.5755914358253624
9	0.2452838317485904	1.3397464039554123
>10	1.2312626051158304	12.813335038282306
>50	0.04465711857606317	1.8049488160933582
>100	0.012050745856140879	1.1763765039692493
>500	1.9590175786077913E-4	0.09624238010391455
>1k	2.9385263679116867E-4	0.45249968953119063
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3073	0.18238536074079437	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2663	0.15805148573144662	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1876	0.11134231589643027	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1870182931389155E-4
2	0.0	0.0	0.0	5.935091465694578E-5	1.1870182931389155E-4
3	0.0	0.0	0.0	5.935091465694578E-5	1.1870182931389155E-4
4	0.0	0.0	0.0	5.935091465694578E-5	1.1870182931389155E-4
5	0.0	0.0	0.0	5.935091465694578E-5	1.1870182931389155E-4
6	0.0	0.0	0.0	5.935091465694578E-5	1.7805274397083732E-4
7	0.0	0.0	0.0	5.935091465694578E-5	1.7805274397083732E-4
8	0.0	0.0	0.0	5.935091465694578E-5	2.374036586277831E-4
9	0.0	0.0	0.0	1.1870182931389155E-4	2.374036586277831E-4
10-11	0.0	0.0	0.0	1.1870182931389155E-4	2.374036586277831E-4
12-13	0.0	0.0	0.0	1.4837728664236443E-4	5.044827745840392E-4
14-15	0.0	0.0	0.0	1.7805274397083732E-4	8.30912805197241E-4
16-17	0.0	0.0	0.0	1.7805274397083732E-4	0.0010089655491680783
18-19	0.0	0.0	0.0	2.0772820129931022E-4	0.001068316463825024
20-21	0.0	0.0	0.0	2.9675457328472886E-4	0.0012463692077958612
22-23	0.0	0.0	0.0	3.5610548794167465E-4	0.0014244219517666986
24-25	0.0	0.0	0.0	9.199391771826595E-4	0.0015431237810805902
26-27	0.0	0.0	0.0	0.0019289047263507378	0.0016321501530660088
28-29	0.0	0.0	0.0	0.0049261259165265	0.0016618256103944817
30-31	0.0	0.0	0.0	0.013828763115068365	0.0016618256103944817
32-33	0.0	0.0	0.0	0.03317716129323269	0.001839878354365319
34-35	0.0	0.0	0.0	0.055878886149514445	0.001958580183679211
36-37	0.0	0.0	0.0	0.0900946884492437	0.001958580183679211
38-39	0.0	0.0	0.0	0.14264992337796917	0.0020179310983361566
40-41	0.0	0.0	0.0	0.19941907324733782	0.0020772820129931024
42-43	0.0	0.0	0.0	0.2575829696111447	0.0020772820129931024
44-45	0.0	0.0	0.0	0.326697109729158	0.002136632927650048
46-47	0.0	0.0	0.0	0.398422690092077	0.002225659299635467
48-49	0.0	0.0	0.0	0.4709495078028647	0.0022553347569639398
50-51	0.0	0.0	0.0	0.5443369137761782	0.0022553347569639398
52-53	0.0	0.0	0.0	0.6202764090797404	0.0024333875009347767
54-55	0.0	0.0	0.0	0.713190265975189	0.002611440244905614
56-57	0.0	0.0	0.0	0.8125733725682447	0.00267079115956256
58-59	0.0	0.0	0.0	0.9200578790119734	0.00267079115956256
60-61	0.0	0.0	0.0	1.0243671115215558	0.0027301420742195056
62-63	0.0	0.0	0.0	1.1310800560747443	0.002908194818190343
64-65	0.0	0.0	0.0	1.247111094229073	0.0029378702755188156
66-67	0.0	0.0	0.0	1.382965337878822	0.0030268966475042345
68-69	0.0	0.0	0.0	1.5273364377818428	0.0030862475621611803
70-71	0.0	0.0	0.0	1.6818268686338724	0.0033533266781174366
72-73	0.0	0.0	0.0	1.8323111127465586	0.003739107623387584
74-75	0.0	0.0	0.0	1.9958228826264444	0.003739107623387584
76-77	0.0	0.0	0.0	2.185122624924773	0.0037687830807160566
78-79	0.0	0.0	0.0	2.394839081865091	0.0037984585380445297
80-81	0.0	0.0	0.0	2.607345031794285	0.003946835824686894
82-83	0.0	0.0	0.0	2.826260880506429	0.0045106695139278796
84-85	0.0	0.0	0.0	3.055770867484839	0.0045106695139278796
86-87	0.0	0.0	0.0	3.313442863467969	0.004540344971256352
88-89	0.0	0.0	0.0	3.604677801689602	0.004570020428584825
90-91	0.0	0.0	0.0	3.9088215638491204	0.0045996958859132984
92-93	0.0	0.0	0.0	4.207861147348142	0.0046887222578987165
94-95	0.0	0.0	0.0	4.528296735580993	0.004807424087212608
96-97	0.0	0.0	0.0	4.877903298367731	0.004866775001869553
98-99	0.0	0.0	0.0	5.270479923366099	0.004866775001869553
100-101	0.0	0.0	0.0	5.6867375633125885	0.004866775001869553
102-103	0.0	0.0	0.0	6.101748834051282	0.005163529575154283
104-105	0.0	0.0	0.0	6.53474343193103	0.005193205032482755
106-107	0.0	0.0	0.0	7.0070283353136755	0.005400933233782065
108-109	0.0	0.0	0.0	7.511422083525729	0.005727363264395267
110-111	0.0	0.0	0.0	8.052316644251805	0.005875740551037631
112-113	0.0	0.0	0.0	8.599116620986246	0.0059350914656945775
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2780	0.0	57.94048	1
GTATCAA	4795	0.0	50.512695	1
TCAACGC	6210	0.0	37.745106	4
TATCAAC	6330	0.0	37.50179	2
CAACGCA	6290	0.0	37.217415	5
ATCAACG	6425	0.0	36.57596	3
AACGCAG	6575	0.0	35.797085	6
GTACATG	8635	0.0	33.300274	1
TACATGG	8975	0.0	31.6998	2
ACATGGG	9260	0.0	30.917393	3
CATGGGG	7735	0.0	30.149225	4
ACGCAGA	7790	0.0	29.98348	7
ATGGGGG	4610	0.0	29.7188	5
CGCAGAG	8300	0.0	28.140285	8
GCAGAGT	9055	0.0	25.662365	9
GAGTACT	4040	0.0	25.05035	12-13
GTATCGC	180	2.15356E-5	23.172445	6
AGAGTAC	8295	0.0	23.129168	10-11
GTACTTT	4420	0.0	22.957567	14-15
CAGAGTA	8695	0.0	22.407772	10-11
>>END_MODULE