FastQCFastQC Report
Mon 15 Aug 2016
SRR2921597_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921597_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571428
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG2650.062.8869553
GCGTAGC4700.046.8546072
GTATCAA29800.040.156211
TCAACGC34050.034.9591874
GGTATCA19100.034.9106251
GTACATG81400.034.0827371
GTAGCGT3700.033.7804954
CAACGCA35150.033.524375
TACATGG84150.032.8180122
ACATGGG84450.032.3490453
ATCAACG37600.031.6585183
TATCAAC37400.031.5095392
AACGCAG37650.031.4523816
CATGGGG70900.031.3120164
GGCGTAG4950.031.270951
ATGGGGG45600.028.321465
TAGCGTC4000.028.2692575
ACGCAGA46150.025.64887
CGCAGAG48350.024.3556148
GAGTACA58950.021.7133881
Produced by FastQC (version 0.11.5)